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131.
132.
A survey of ovine schistosomiasis was carried out in a Schistosoma bovis enzootic area in the central Sudan. Three hundred Desert sheep, representing different age groups, were screened for infection, using Pitchford's faecal egg counting technique. No infection was detected in animals under one year old, but from the age of 18 months onward, the prevalence increased progressively from around 20 per cent to 60 per cent in six-year-old sheep, and this was accompanied by an increase in infection intensity, as determined by faecal egg counts. These findings, which suggest that these sheep did not develop an effective resistance to schistosomiasis, contrasted with those previously recorded in cattle from the same locality, studied in the same year. 相似文献
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Imada T Abdul Rahman MA Kashiwazaki Y Tanimura N Syed Hassan S Jamaluddin A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):81-83
Eight clones of monoclonal antibodies (Mabs) to Nipah virus (NV) were produced against formalin-inactivated NV antigens. They reacted positive by indirect immunofluorescent antibody test, and one of them also demonstrated virus neutralizing activity. They were classified into six different types based on their biological properties. These Mabs will be useful for immunodiagnosis of NV infections in animals and further research studies involving the genomes and proteins of NV. 相似文献
136.
Naeem AbbasSarfraz Ali Shad Muhammad Razaq 《Pesticide biochemistry and physiology》2012,103(3):181-188
Imidacloprid has been used as a key insecticide for controlling sucking insect pests of cotton, whereas Spodoptera litura also has been indirectly exposed to this insecticide in Pakistan. To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field collected population was selected with imidacloprid in the laboratory. Thereafter, fitness cost, realized heritability and cross resistance of imidacloprid resistance in S. litura were investigated. After 14 generations of selection with imidacloprid, S. litura developed a 137.48-fold resistance to the insecticide. Bioassay revealed that this strain showed cross-resistance to acetamiprid (RR 8.52) and a little to lamdacyhalothrin (1.92) but negative cross-resistance was found to methomyl (−0.19). The resistant strain had a relative fitness of 0.38, with substantially lower rates of larval survival, larval duration, male pupal duration, development time, emergence rate of healthy adults, fecundity, hatchability, and prolonged larval and pupal duration. Mean relative growth rate of the larvae, intrinsic rate of population increase, and biotic potential was lower for the selected populations. The estimated realized heritability (h2) of imidacloprid resistance was 0.15 in the resistant strain of S. litura. Development of the resistance may cost significant fitness for the resistant population. This study provided valuable information for further understanding the impact of imidacloprid resistance on physiological parameters of S. litura and for facilitating the development of resistance management strategies. 相似文献
137.
Shahid Hussain Muhammad Aamer Maqsood Zed Rengel Muhammad Khawar Khan 《Euphytica》2012,186(1):153-163
Estimating variation in grain mineral concentration and bioavailability in relation to grain yield and the year of cultivar
release is important for breeding wheat with increased content of bioavailable minerals. The grain yield and yield components,
grain phytate concentration, and concentration and bioavailability of minerals (zinc Zn, iron Fe and calcium Ca) in wheat
grains were estimated in 40 wheat cultivars released in Punjab (Pakistan) during the last five decades. Mean grain Zn and
Ca concentrations in current-cultivars were significantly lower (≥14%) than in obsolete cultivars released during the Green
Revolution (1965–1976). Much of this variation was related to increased grain weight in current-cultivars. There was a positive
correlation among minerals (r = 0.39 or higher, n = 40) and minerals with phytate in wheat grains (r = 0.38 or higher, n = 40). The tested cultivars varied widely in grain yield and grain phytate-to-mineral molar ratios (phytate:mineral). Compared
to obsolete cultivars, the current-cultivars had a higher phytate:mineral ratio in grains, indicating poor bioavailability
of minerals to humans. The study revealed a non-significant relationship between grain yield and phytate:mineral ratios in
grains. Therefore, breeding for lower phytate:mineral ratios in wheat grains can ensure increased mineral bioavailability
without significant reduction in the yield potential. Future breeding should be focused on developing new genotypes suitable
for mineral biofortification and with increased mineral bioavailability in grains. 相似文献
138.
Molecular markers have been used for identification and mapping of genes and QTLs for numerous agriculturally important traits
in tomato, including resistance/tolerance to biotic and abiotic stresses and fruit- and flower-related characteristics. However,
the extent to which markers have been utilized in tomato breeding programs has not been clearly determined. A review of the
literature indicated that the utility of most markers for use in tomato breeding programs have not been verified. Many markers
are not validated across tomato genotypes or are not polymorphic within tomato breeding populations. In this study, we examined
the utility of available markers for several major disease resistance traits in tomato by testing them in a number of breeding
lines and commercial cultivars with known resistance/susceptibility responses. While several markers were validated, others
needed PCR optimization for successful amplifications or were not informative in the genotypes used. Specifically, of the
37 markers examined 19 (~51%) were informative, including markers for resistance to Fusarium wilt, late blight, bacterial
wilt, tomato mosaic virus, tomato spotted wilt virus, and root knot nematodes. It appears that many of the available markers
may need to be further refined or examined for trait association and presence of polymorphism in breeding lines and populations.
However, with recent advances in tomato sequencing, it is becoming increasingly possible to develop more informative markers
to accelerate the use of MAS in tomato breeding. 相似文献
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140.
Mahr-un-Nisa Shahzad MA Phillips CJ Sarwar M 《Tropical animal health and production》2012,44(5):947-952
Urea is a common ingredient of the diets of intensively fed lambs, but is increasingly required for industrial processes.
Maize steep liquor (MSL) is a by-product of maize grain degradation to produce starch that may be a suitable replacement.
Fifty growing lambs were fed on equinitrogenous diets in which between 0% and 80% of the urea was replaced by MSL; their growth
and metabolism were recorded over 70 days. Increasing replacement of urea by MSL increased feed intake and nutrient digestibilities,
leading to increased growth rates, more efficient feed conversion, and increased nitrogen retention. Concentrations of triiodothyroxin,
thyroxin, glucose, and methionine were increased by replacement of urea by liquor, and plasma urea was reduced. This study
suggests that MSL is a suitable replacement for up to 80% of urea in the diet of rapidly growing lambs. 相似文献