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991.
Ergot, caused by Claviceps africana , has emerged as a serious threat to sorghum hybrid seed production worldwide. In the absence of gene-for-gene-based qualitative resistance in commercial cultivars, varieties with high pollen production that can escape ergot infection are preferred. Recent demonstration of differences in ergot susceptibility among male-sterile lines has indicated the presence of partial resistance. Using chitin-specific fluorescin-isothiocyanate-conjugated wheat germ agglutin and callose-specific aniline blue, this study investigated the process of sorghum ovary colonization by C. africana . Conidia germinated within 24 h after inoculation (a.i.); the pathogen was established in the ovary by 79 h a.i., and at least half of the ovary was converted into sphacelial tissue by 120 h a.i. Changes in fungal cell wall chitin content and strategic callose deposition in the host tissue were associated with penetration and invasion of the ovary. The rate of ovary colonization differed in three male-sterile lines that also differed in ergot susceptibility. This work demonstrates a possible histological basis for partial resistance in male-sterile sorghum lines that could lay the foundation for variety improvement through further breeding and selection. 相似文献
992.
Single-copy restriction fragment length polymorphism (RFLP) markers were used to determine the genetic structure of the global population of Mycosphaerella musicola , the cause of Sigatoka (yellow Sigatoka) disease of banana. The isolates of M. musicola examined were grouped into four geographic populations representing Africa, Latin America and the Caribbean, Australia and Indonesia. Moderate levels of genetic diversity were observed for most of the populations ( H = 0·22–0·44). The greatest genetic diversity was found in the Indonesian population ( H = 0·44). Genotypic diversity was close to 50% in all populations. Population differentiation tests showed that the geographic populations of Africa, Latin America and the Caribbean, Australia and Indonesia were genetically different populations. Using F ST tests, very high levels of genetic differentiation were detected between all the population pairs ( F ST > 0·40), with the exception of the Africa and Latin America-Caribbean population pair. These two populations differed by only 3% ( F ST = 0·03), and were significantly different ( P < 0·05) from all other population pairs. The high level of genetic diversity detected in Indonesia in comparison to the other populations provides some support for the theory that M. musicola originated in South-east Asia and that M. musicola populations in other regions were founded by isolates from the South-east Asian region. The results also suggest the migration of M. musicola between Africa and the Latin America-Caribbean region. 相似文献
993.
ABSTRACT Snap bean plants within seven-row segments that ranged from 65 to 147 m were sampled, using a cyclic sampling plan. In the cyclic sampling plan, only 6 of every 31 plants were sampled, but sampled plants were spaced such that pairs of plants that were 1, 2, 3, 4,..., 1,525 plants apart could be identified within each sample. Every leaflet on every sampled plant was assessed for bacterial brown spot, and the proportion of disease leaflets per plant was determined. Arcsine square-root-transformed disease incidence values were analyzed for spatial patterns by autocorrelation and spectral analyses. Disease patterns were detected at several different scales within a single snap bean row, at distances that ranged from 20 to 100 m. Approximately 23 to 53% of the disease variability in the samples could be described by sine and cosine curves, indicating a substantial component of regularity in the disease patterns. Possible origins for these regular patterns, including cultural practices and seed infestation, are discussed. 相似文献
994.
The effects of adjuvants on the performance of a dispersible concentrate formulation (DCI) of a novel powdery mildew fungicide, 1-(4-chlorobenzyl)-4-phenylpiperidine (I) were investigated. The method involved assessment, under glasshouse conditions, of the therapeutic (curative) control of infections of powdery mildew (Erysiphe graminis DC f.sp. hordei Marchal) on barley (Hordeum vulgare, L.) eight to nine days after spray application to plants that had been inoculated one day prior to spraying. The results from the first trial showed that marked improvements (∽five-fold) in the performance of DCI could be obtained by the spray tank addition of some types of surfactant adjuvants and a series of further trials investigating a wide range of adjuvants was conducted. Nonylphenol, alkylamine and alcohol ethoxylates varying in mean ethylene oxide content between 5 and 20 moles were highly effective. There were indications that optimum performance enhancements were obtained with these surfactants containing between 5 and 10 moles ethylene oxide. Lower enhancements, sometimes only marginal, were obtained from trisiloxane, phosphate ester, propylene oxide, alkylamine-propylene oxide and castor oil ethoxylates and also alkyl polyglucoside biosurfactants. Negligible adjuvant enhancements were observed with emulsifiable paraffinic/naphthenic and rape seed oils, though slightly better enhancements were seen with an emulsifiable transmethylated rape seed oil and, interestingly, larger enhancements with an emulsifiable lipophilic alcohol. A final trial identified the alcohol ethoxylate, ‘Dobanol’ 91-6, as the most effective adjuvant and that its optimum application rate under glasshouse conditions was 250 g ha-1. This information will be used to guide the design of field trials. © 1997 SCI 相似文献
995.
Spatial genetic structure and dispersal of the cacao pathogen Moniliophthora perniciosa in the Brazilian Amazon 下载免费PDF全文
A. S. Artero J. Q. Silva P. S. B. Albuquerque E. A. Bressan G. A. Leal Jr A. M. Sebbenn G. W. Griffith A. Figueira 《Plant pathology》2017,66(6):912-923
Moniliophthora perniciosa is the causal agent of witches’ broom in Theobroma cacao (cacao). Three biotypes of M. perniciosa are recognized, differing in host specificity, with two causing symptoms on cacao or Solanaceae species (C‐ and S‐biotypes), and the third found growing endophytically on lianas (L‐biotype). The objectives of this study were to clarify the genetic relationship between the three biotypes, and to identify those regions in the Brazilian Amazon with the greatest genetic diversity for the C‐biotype. Phylogenetic reconstruction based on the rRNA ITS regions showed that the C‐ and S‐biotypes formed a well‐supported clade separated from the L‐biotype. Analysis of 131 isolates genotyped at 11 microsatellite loci found that S‐ and especially L‐biotypes showed a higher genetic diversity. A significant spatial genetic structure was detected for the C‐biotype populations in Amazonia for up to 137 km, suggesting ‘isolation by distance’ mode of dispersal. However, in regions containing extensive cacao plantings, C‐biotype populations were essentially ‘clonal’, as evidenced by high frequency of repeated multilocus genotypes. Among the Amazonian C‐biotype populations, Acre and West Amazon displayed the largest genotypic diversity and might be part of the centre of diversity of the fungus. The pathogen dispersal may have followed the direction of river flow downstream from Acre, Rondônia and West Amazon eastward to the rest of the Amazon valley, where cacao is not endemic. The Bahia population exhibited the lowest genotypic diversity, but high allele richness, suggesting multiple invasions, with origin assigned to Rondônia and West Amazon, possibly through isolates from the Lower Amazon population. 相似文献
996.
S. P. Dendy B. Tong H. M. Alexander P. A. Fay L. Murray Y. Xing K. A. Garrett 《Plant pathology》2017,66(8):1308-1317
Tallgrass prairie species have evolved with regular exposure to fire. However, burning has been used as a management tool for reducing plant disease in agricultural systems, posing the question of how plant pathogens of tallgrass prairie would be affected by burning. The rust fungus Puccinia dioicae, infecting Erigeron strigosus (Asteraceae), was studied for 8 years in long‐term experiments to evaluate the effects of burning in native tallgrass prairie. This experiment also allowed evaluation of the effects of nutrient additions, although E. strigosus was rare in the plots with added nutrients in most years. Burning reduced rust severity in most years, but effects from additions of nutrients were rarely observed. There was high interannual variation in rust severity within a location, suggesting that weather may be the most important of these three abiotic factors in determining infection. An analysis of weather variables associated with disease severity found that solar radiation in the month prior to sampling was associated with severity in unburned plots; temperature approximately 2 months prior to sampling was also associated with severity in burned plots. High interannual variation also suggests that the effects of this pathogen on its host would be sporadic and difficult to study in short‐term experiments. 相似文献
997.
Spatial organization of multiple plant species that appears as a non-random distribution of vegetative patches is one of the mostly observed spatial patterns in arid ecosystems. Yet understanding of ecological processes allowing this spatial pattern to emerge through interspecific interactions is still lacking. With a proposed conceptual model involving interspecific trade-offs between species competitive ability and colonization ability, we have argued that within patch abundance dynamics regulated by the mechanisms of competition are strongly influenced by the between patches colonization dynamics that are maintained via this trade-offs and it holds a positive, intraspecific occupancy-abundance relationship, in which increased patch occupancy increases species density within inhabiting patches. In a constant environment, while local abundance dynamics approach toward a stable equilibrium point, a fixed spatial arrangement of species can be retained through this coupled dynamics. However, in fluctuating environments where existence of such stable equilibriums is highly uncertain, it may involve continuous transitions from one community state to another as species re-organized themselves over space through the rapid changes in local species abundances. While some of the inhabiting patches are destroyed exogenously or endogenously, or species responses to increasing environmental fluctuations vary increasingly with time, discontinuous transitions into an abrupt, irreversible state of the community dynamics may occur, as with this effect the inherent positive relationship between occupancy and abundance of species is no longer maintained. 相似文献
998.
Between 2005 and 2007 a total of 1364 oilseed rape (OSR) fields in 12 federal states of Germany was surveyed. Investigations took place in late autumn and included the identification and counting of all weeds in unsprayed parts of rape fields. Results on the impact of tillage intensity and crop sowing date on the level of total weed infestation, weed species number and weed flora composition in OSR fields are shown and those species identified which are sensitive to the analysed factors. Neither tillage intensity nor crop sowing date affect the level of weed infestation in OSR fields. In contrast, species richness was enhanced by non-inversion tillage as well as by an early crop sowing. While there were statistically proofed differences in the weed species composition of ploughed and unploughed OSR fields due to canonical correspondence analysis, the crop sowing date only had minor effects on species composition. Nevertheless, a multitude of single weed species was affected by tillage intensity and sowing date showing significantly differing relative frequencies and/or densities in fields with inversion and non-inversion tillage as well as between early and late sown crop. Among others Centaurea cyanus, Anchusa arvensis, A. spica-venti and Elymus repens were more abundant and partly reached higher densities after ploughing, whereas non-inversion tillage mainly favoured Galium aparine, Geranium spp. and Sisymbrium spp.. Lots of weed species tended to have higher populations when OSR was sown before August 15th, species significantly increased by early sowing date were e. g. Centaurea cyanus, Convolvulus arvensis, Euphorbia spp. and Anchusa arvensis. However, only V. arvensis reached significantly higher densities when OSR was sown after September 1st. 相似文献
999.
P. D. Roberts S. Adkins K. Pernezny J. B. Jones 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(9):913-920
Volume Contents
Volume Contents 相似文献1000.
Mohamed Béchir Allagui Philippe Lepoivre 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(9):887-894
Isolates of Phytophthora from pepper, produced in Tunisia, were characterised according to molecular and pathogenicity criteria. Polymerase chain reaction amplification of the ITS1 region in the ribosomal DNA resulted in different sized fragments. The pepper isolates and P. nicotianae yielded a fragment of 310bp that distinguished it from P. capsici with a fragment of 270bp. The ribosomal RNA gene amplicons of both internal transcribed spacers and the 5.8 S of the pepper Phytophthora and P. nicotianae were digested with 8 endonucleases. The patterns generated, with the 2 enzymes that cut, were identical for both taxa. This molecular analysis corroborated the morphological and biological characteristics and suggests strongly that the isolates of Phytophthora from pepper belong to the species P. nicotianae. Inoculation of pepper, tomato, eggplant and tobacco plants with the isolates of P. nicotianae from pepper showed they were highly pathogenic on pepper but not on tobacco, while their pathogenicity was weak on tomato and eggplant and was associated with atypical symptoms not observed in the field. These pathogenicity tests suggest that pepper isolates of P. nicotianae are particularly adapted to their host and may thus constitute a forma specialis of P. nicotianae. 相似文献