Assessment of genetic variability in the endangered <Emphasis Type="Italic">Citrus macroptera</Emphasis> Mont. (“hatkora”) from Mizoram,north-east India

Assessment of genetic variability in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In view of this, 39 “hatkora” (Citrus macroptera Mont.) genotypes collected from different locations of Mizoram, India were evaluated for physico-chemical characteristics during 2013–2015. The study reveals that there was significant variation among the collections in these particular traits. Individual fruit weight ranged from 277.78 to 617.69 g; fruit diameter 9.32–12.52 cm; fruit length 7.74–10.19 cm, fruit volume 238.33–583.33 cc; pulp weight 153.75–320.94 g; pulp:peel ratio 1.50–3.24 and seed number 9.33–23.6. Similarly, the chemical parameters also varied significantly among different germplasms. The juice content varied from 13.45 to 32.53 %, ascorbic acid 34.81–73.64 mg/100 mL, TSS 6.15–9.10 %, acidity 5.03–8.75 %, total sugars 5.16–7.97 % and sugar:acid ratio 0.75–1.52. From the analysis of genetic parameters, it could be concluded that characters like fruit weight, fruit length, fruit diameter, fruit volume, pulp weight, pulp–peel ratio, juice, Total soluble solids (TSS), acidity, ascorbic acid, total sugars, and sugar–acid ratio could be used as selection criteria for development of effective and productive plant types in “hatkora”. Wide range of variation in physico-chemical parameters of “hatkora” fruits indicated the great scope of individual plant selection based on these characters for future genetic improvement programme.     

Physiological response of cauliflower in relation to manganese-boron interaction

Summary

Cauliflower (Brassica oleracea L. var. botrytis) was grown in refined sand at low (0.0011 mg l^?1), normal (0.55 mg l^?1) and excess (5.5 mg l^?1) Mn, each at three levels of B, deficient (0.0033 mg l^?1), normal (0.33 mg H) and excess (3.3 mg l^?1). In Mn deficient cauliflowers a deficiency of B accentuated visible symptoms of Mn deficiency and aggravated the Mn deficiency effects i.e. a decrease in dry matter, leaf Mn, sugars, starch, chlorophyll, Hill reaction and specific activity of aldolase and an increase in the concentrations of proline and inorganic phosphorus. In cauliflowers exhibiting B toxicity symptoms under conditions of excess B, excess Mn increased leaf Mn, sugars, nucleic acids, protein P, nucleic acid P, acid-labile P and leaf B. Excess Mn decreased the concentrations of DNA, protein nitrogen, chlorophyll and activities of peroxidase, aldolase and leaf B, leaf Mn in boron deficient cauliflowers.     

Genetics of hyperpigmentation associated with the Fibromelanosis gene (Fm) and analysis of growth and meat quality traits in crosses of native Indian Kadaknath chickens and non-indigenous breeds

1. The study investigated the extent of hyperpigmentation (a trait fixed in native Indian Kadaknath chickens), bodyweight, carcase quality and leanness at 12 weeks of age in F(1) and back-crosses of Kadaknath with White Leghorn, White Plymouth Rock and Aseel Peela chickens. 2. The objective of the study was to determine if hyperpigmentation was affected by the major gene Fibromelanosis (Fm) and to evaluate the effects of different proportions of Kadaknath genes on growth and carcase quality. 3. The pigmentation pattern of skin indicated that Fm behaved as the primary locus affecting dermal-hyperpigmentation and that the sex-linked Id locus produced an epistatic effect. 4. The results suggested that variable allelic forms of Id were acting in different crosses, which resulted in variation in melanosis of the host. However, no conclusive pattern for shank pigmentation could be explained through genotyping of the Id and Fm loci. 5. Analysis of quantitative traits indicated the positive impact of a Kadaknath genomic proportion of 50% or more on meat texture and carcase leanness. Improvement in leanness occurred in White Rock crosses but not in White Leghorn and Aseel Peela crosses. 6. Thigh-meat texture was influenced more by enhanced Kadaknath genomic proportions than the breast-meat. It was concluded that introgression of Kadaknath genomic proportion beyond 50% in a cross with meat-type chickens, irrespective of the impact Fm, brought improvement in meat quality whereas no such advantage was obtained for growth traits. 7. The beneficial impact of the Kadaknath genome on meat quality calls for further studies to identify causative genes for their selective use to improve meat quality in Kadaknath crossbred chickens.     

Developmental aberrations in seeds of boron deficient sunflower and recovery

Sunflower (Helianthus annuus L.) cv. Modern grown in refined sand at deficient (0.033 mg L^‐1) boron (B) developed visible symptoms of low B accompanied by marked depression in growth, dry matter, tissue B, flower head size, and seed weight. The B deficient seeds showed a marked decrease in non‐reducing sugars and contents of oil and starch whereas in leaves reducing sugars accumulated. Except for slight increase in leaf B and flower size, resumption of sufficient B (0.33 mg L^‐1) to B deficient plants from the day of anthesis could not appreciably alter the growth, dry matter, head size and seed weight of deficient plants. Apart from this, non‐reducing sugar content in seeds increased on resupplying B to deficient plants. A significant decrease in non‐reducing sugars and starch content in B sufficient seeds by withdrawing B from the day of anthesis indicate a specific role of B in production and deposition of reserve in the seeds of sunflower.     

Prevalence and risk factors for medical events following exercise at Australian Greyhound race meetings

Aim

A prospective, observational study to determine the prevalence of post‐exercise conditions at Australian Greyhound race meetings and to assess association with race performance and other environmental, race‐ and dog‐related factors was undertaken.

Methods

A total of 4020 starters were observed (2813 Greyhounds, 1009 trainers, 536 races, 52 race meets, 48 race dates and 11 race tracks) following a race. The presence of diaphragmatic flutter (DF), ataxia, seizure, collapse or sudden death was recorded. Risk factors were screened by univariable logistic regression prior to multivariable backward stepwise model building.

Results

In this study, 962 starters (n = 768 dogs) had DF (23.9%), 16 starters were ataxic (0.4%) and there were no observed cases of collapse, seizure or sudden death. Race track location, increasing race distance, race grade based on increasing 1st place prize value, lower (earlier) race number at the meeting, age, a previous observation of DF at the last start, females, colour (white) and better finishing position were all associated with an increased risk of a Greyhound being observed with DF. However, when logistic regression assessing the random effect of dog was performed, the presence of previous DF was not significant. In this cohort, DF was common following strenuous exercise in Greyhounds and on its own does not appear to result in reduced performance or distress to the animal.

Conclusion

The incidence of ataxia was low and collapse, seizure and sudden death were not observed. However, even though uncommon, ataxia has welfare concerns for racing Greyhounds that warrants further investigation.     

Prospective determination of the specificity of a commercial snake venom detection kit in urine samples from dogs and cats

Objective To determine the specificity of a snake venom detection kit in urine samples from dogs and cats presenting to a referral centre for diseases unrelated to snake envenomation. Design Urine was collected from 50 dog and 25 cats presented for investigation and treatment of diseases unrelated to snake envenomation. Urine was collected as a voided sample, by cystocentesis or by catheterisation, and routine urinanalysis was performed. Snake venom testing was performed within 2 h of collection using a commercially available snake venom detection kit, which was observed continuously during the 10-min colour reaction phase for evidence of a visible colour indicating a positive test. Results No false-positive reactions occurred in any sample analysed. Conclusion The snake venom detection kit appears to have 100% specificity for using urine as a test sample.     

Present and future feasibility of short-rotation energy farms in Ontario

The economic feasibility of energy farming based on Populus × euramericana (clone I45/51) in eastern Ontario has been evaluated for two potential energy end-uses: direct combustion and methanol production. Optimal management regime and the time when energy farming should begin to be economically most desirable have been determined. Three-year rotations with 16–35 thousand stumps/ha planted and coppiced for 5–6 rotations could be an attractive enterprise in about 15 years. Farming for methanol production could be feasible only about 35 years from now.     

Underutilized and unexploited fruits of Indo-Burma hot spot,Meghalaya, north-east India: ethno-medicinal evaluation,socio-economic importance and conservation strategies

An attempt has been made during 2012–2014 at to enumerate the ethno-medicinal evaluation and socio-economic importance of underutilized and unexploited fruits among the Garo tribe of west Garo hills district, Meghalaya, north-east India. The study was based on extensive field surveys, sample collection and interviews with the traditional healers as well as old aged men and women and survey in rural markets regarding their market demand, price and season of availability. Altogether 66 underutilized and unexploited fruits belonging to 34 families have been enumerated in this paper. The documented fruits were mostly used by the rural people to cure a number of ailments. In addition, these fruits also have socio-economic importance in the rural life of the tribal people like use as constructional timbers, firewood, charcoal, fodder, making dye, yielding oil and a number of value added products. Although, these fruits play an important role in the social and cultural life of Garo people, but these valuable species have been vanishing at very faster rate due to lack of proper documentation. Therefore, the claimed therapeutic values of these species are to be critically studied to establish their safety and effectiveness and both in situ and ex situ conservation measures should be immediately undertaken to safeguard the valuable indigenous rural resources of the world.     

Growth Parameters,Leaf Characteristics and Nutrient Status of Banana as Influenced by Organics,Biofertilizers and Bioagents

Nutrients play an important role in improving productivity and quality of banana. It has been observed that the nutrient applied alone was less effective in improving banana production as compared to nutrients applied in combination with organic manures and biofertilizers. Keeping in view the conservation of soil health and quality production on a long-term basis, a field experiment was conducted during 2009–2011 to find out the influence of Integrated Nutrient Management of banana on leaf characteristics, growth and nutrient status of tissue cultured banana cv. ‘Grand Naine’. The study indicates that combination of inorganic fertilizers with organic manures, biofertilizers and bioagents significantly increased growth parameters, leaf characteristics, and leaf nutrient status of banana. The leaf characteristics in terms of functional leaves, total number of leaves, phyllochron, leaf area, and leaf area index were significantly influenced by the combination of inorganic fertilizers with different biofertilizers and organic manures. Similarly, leaf nutrient status like nitrogen, phosphorus pentoxide_, (P₂O₅), potassium dioxide (K₂O), and leaf relative water content were also influenced greatly by different nutrients. Treatment involving combination of 100% recommended dose of fertilizer (RDF) + arbuscular mycorrhizal fungi (AMF) + Azospirillum+ Trichoderma harzianum showed overall superiority in most of the parameters.