Anti-diabetic potential of ursolic acid stearoyl glucoside: a new triterpenic gycosidic ester from Lantana camara

A new stearoyl glucoside of ursolic acid, urs-12-en-3β-ol-28-oic acid 3β-d-glucopyranosyl-4′-octadecanoate and other compounds were isolated from the leaves of Lantana camara L. The structure of this new glycoside was elucidated and established by standard spectroscopic methods. In streptozotocin-induced diabetic rats it showed significant reduction in blood glucose level.     

Effects of intensification of pastoral farming on greenhouse gas emissions in New Zealand

Abstract

AIMS: The aims of this cross-sectional study were to investigate the herd and cow-level prevalence of bovine digital dermatitis (BDD) in dairy farms in the northern Taranaki region of New Zealand, and to identify whether there was any spatial clustering of herds with the disease.

METHODS: A survey of 224 dairy farms in the northern Taranaki region of New Zealand was undertaken from September 2014 to February 2015. Following training in robust criteria to confirm BDD visually, a technician inspected the rear feet of every milking cow on the farms during milking. The identity of cows with lesions and the feet involved were recorded. The proportion of cows affected among the inspected population (cow-level prevalence), the proportion of a herd affected (farm-level prevalence), and proportion of farms with ≥1 cow with lesions, were calculated. A bivariate K function analysis was then used to assess whether farms with ≥1 cow with lesions were clustered, after accounting for the distribution of the farms involved in the study.

RESULTS: Bovine digital dermatitis lesions were observed on 143/224 (63.8 (95% CI=57.5–70.1)%) farms. Within-farm prevalence was 0% on 81 (36.2%) farms, between >0 and <3% on 120 (53.5%) farms, with a maximum prevalence of 12.7% on one farm. Overall, cow-level prevalence was 707/60,455 (1.2 (95% CI=0.9–3.0)%), and on affected farms was 707/41,116 (1.7 (95% CI=1.4–2.1)%). In affected cows, 268/707 (37.9%) had a lesion on left foot only, 262/707 (37.1%) on the right foot only and 177/707 (25.0%) on both feet. The K function analysis showed no evidence of clustering of farms with BDD.

CONCLUSIONS: Bovine digital dermatitis was widespread among the survey farms, but there was no evidence that there was any clustering of herds with BDD. The cow-level prevalence on affected farms was much lower than reported elsewhere.

CLINICAL RELEVANCE: Although the prevalence at the cow level was low, if these data are representative of other regions of New Zealand, BDD could easily become a major problem on dairy farms in New Zealand, as has been observed in other countries.     

Synaptonemal complex investigations on lamas (Lama glama) with differing fertility recordings

The present study involved three male lamas ( Lama glama ). Mitotic preparations from fibroblast culture and an electron microscopic observation on the synaptonemal complexes (Scs) were reported. Special attention was given to the morphology and behaviour of the chromosomes at the zygotene and pachytene substages of prophase I in primary spermatocytes from lamas. Analysis of mitotic preparations show diploid and triploid cells, with a relatively high frequency of polyploidy. Analyses of synaptonemal complexes in primary spermatocytes were carried out on 89 cells. Pairing abnormalities were only recorded in an average of 63% of the cells of the animal Tabasco. The other two animals were normal. The photographed cells give an upper limit estimate of the existing abnormalities, as there was a deliberate tendency towards selecting abnormal cells for photography. The presence of degenerating primary spermatocytes in SC preparations as well as in testicular sections, and the absence of spermatozoa in ejaculates confirm the chromosomally derived male sterility of one animal.     

Surgical resection of a dysgerminoma in a mare

A mare was referred for further evaluation of a mass found in the left caudal abdomen during a routine postpartum reproductive palpation. The mare was clinically normal with no history of health problems. Ultrasonographic examination of the mass confirmed its presence, but the origin of the mass could not be accurately determined. Routine haematology and serum biochemistry results were within normal limits. The mare was initially treated conservatively with antibiotics, but the mass continued to increase in size, so it was surgically excised. The mass involved the left ovary. The mare showed transient abdominal pain after surgery, but developed no other complications and was in foal 7 months later. On histology, the mass was diagnosed as a dysgerminoma, a rare ovarian tumour of germ cell origin.     

Assessment of genetic variability in the endangered <Emphasis Type="Italic">Citrus macroptera</Emphasis> Mont. (“hatkora”) from Mizoram,north-east India

Assessment of genetic variability in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In view of this, 39 “hatkora” (Citrus macroptera Mont.) genotypes collected from different locations of Mizoram, India were evaluated for physico-chemical characteristics during 2013–2015. The study reveals that there was significant variation among the collections in these particular traits. Individual fruit weight ranged from 277.78 to 617.69 g; fruit diameter 9.32–12.52 cm; fruit length 7.74–10.19 cm, fruit volume 238.33–583.33 cc; pulp weight 153.75–320.94 g; pulp:peel ratio 1.50–3.24 and seed number 9.33–23.6. Similarly, the chemical parameters also varied significantly among different germplasms. The juice content varied from 13.45 to 32.53 %, ascorbic acid 34.81–73.64 mg/100 mL, TSS 6.15–9.10 %, acidity 5.03–8.75 %, total sugars 5.16–7.97 % and sugar:acid ratio 0.75–1.52. From the analysis of genetic parameters, it could be concluded that characters like fruit weight, fruit length, fruit diameter, fruit volume, pulp weight, pulp–peel ratio, juice, Total soluble solids (TSS), acidity, ascorbic acid, total sugars, and sugar–acid ratio could be used as selection criteria for development of effective and productive plant types in “hatkora”. Wide range of variation in physico-chemical parameters of “hatkora” fruits indicated the great scope of individual plant selection based on these characters for future genetic improvement programme.     

Physiological response of cauliflower in relation to manganese-boron interaction

Summary

Cauliflower (Brassica oleracea L. var. botrytis) was grown in refined sand at low (0.0011 mg l^?1), normal (0.55 mg l^?1) and excess (5.5 mg l^?1) Mn, each at three levels of B, deficient (0.0033 mg l^?1), normal (0.33 mg H) and excess (3.3 mg l^?1). In Mn deficient cauliflowers a deficiency of B accentuated visible symptoms of Mn deficiency and aggravated the Mn deficiency effects i.e. a decrease in dry matter, leaf Mn, sugars, starch, chlorophyll, Hill reaction and specific activity of aldolase and an increase in the concentrations of proline and inorganic phosphorus. In cauliflowers exhibiting B toxicity symptoms under conditions of excess B, excess Mn increased leaf Mn, sugars, nucleic acids, protein P, nucleic acid P, acid-labile P and leaf B. Excess Mn decreased the concentrations of DNA, protein nitrogen, chlorophyll and activities of peroxidase, aldolase and leaf B, leaf Mn in boron deficient cauliflowers.     

Genetics of hyperpigmentation associated with the Fibromelanosis gene (Fm) and analysis of growth and meat quality traits in crosses of native Indian Kadaknath chickens and non-indigenous breeds

1. The study investigated the extent of hyperpigmentation (a trait fixed in native Indian Kadaknath chickens), bodyweight, carcase quality and leanness at 12 weeks of age in F(1) and back-crosses of Kadaknath with White Leghorn, White Plymouth Rock and Aseel Peela chickens. 2. The objective of the study was to determine if hyperpigmentation was affected by the major gene Fibromelanosis (Fm) and to evaluate the effects of different proportions of Kadaknath genes on growth and carcase quality. 3. The pigmentation pattern of skin indicated that Fm behaved as the primary locus affecting dermal-hyperpigmentation and that the sex-linked Id locus produced an epistatic effect. 4. The results suggested that variable allelic forms of Id were acting in different crosses, which resulted in variation in melanosis of the host. However, no conclusive pattern for shank pigmentation could be explained through genotyping of the Id and Fm loci. 5. Analysis of quantitative traits indicated the positive impact of a Kadaknath genomic proportion of 50% or more on meat texture and carcase leanness. Improvement in leanness occurred in White Rock crosses but not in White Leghorn and Aseel Peela crosses. 6. Thigh-meat texture was influenced more by enhanced Kadaknath genomic proportions than the breast-meat. It was concluded that introgression of Kadaknath genomic proportion beyond 50% in a cross with meat-type chickens, irrespective of the impact Fm, brought improvement in meat quality whereas no such advantage was obtained for growth traits. 7. The beneficial impact of the Kadaknath genome on meat quality calls for further studies to identify causative genes for their selective use to improve meat quality in Kadaknath crossbred chickens.     

Developmental aberrations in seeds of boron deficient sunflower and recovery

Sunflower (Helianthus annuus L.) cv. Modern grown in refined sand at deficient (0.033 mg L^‐1) boron (B) developed visible symptoms of low B accompanied by marked depression in growth, dry matter, tissue B, flower head size, and seed weight. The B deficient seeds showed a marked decrease in non‐reducing sugars and contents of oil and starch whereas in leaves reducing sugars accumulated. Except for slight increase in leaf B and flower size, resumption of sufficient B (0.33 mg L^‐1) to B deficient plants from the day of anthesis could not appreciably alter the growth, dry matter, head size and seed weight of deficient plants. Apart from this, non‐reducing sugar content in seeds increased on resupplying B to deficient plants. A significant decrease in non‐reducing sugars and starch content in B sufficient seeds by withdrawing B from the day of anthesis indicate a specific role of B in production and deposition of reserve in the seeds of sunflower.