Seed Invigoration by Hydration–Dehydration for Improvement of Seed Quality in Safflower (Carthamus tinctorius L.)

Three midstorage hydration-dehydration treatments viz., soaking, moisture equilibration and moist-sand conditioning followed by drying, each at varying durations for preservation of vigour and viability during storage in safflower var. K.1, were evaluated for their efficacy. Soaking seeds for 3 h followed by dehydration proved to be the best. Maintenance of membrane integrity and counteraction of lipid peroxidation may be the possible reasons for such advantage as evidenced by lower electrical conductance and peroxide values in the best treatment.     

Neuroprotective Effects of the Cultivated Chondrus crispus in a C. elegans Model of Parkinson’s Disease

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in the elderly people, currently with no cure. Its mechanisms are not well understood, thus studies targeting cause-directed therapy or prevention are needed. This study uses the transgenic Caenorhabditis elegans PD model. We demonstrated that dietary supplementation of the worms with an extract from the cultivated red seaweed Chondrus crispus decreased the accumulation of α-synulein and protected the worms from the neuronal toxin-, 6-OHDA, induced dopaminergic neurodegeneration. These effects were associated with a corrected slowness of movement. We also showed that the enhancement of oxidative stress tolerance and an up-regulation of the stress response genes, sod-3 and skn-1, may have served as the molecular mechanism for the C. crispus-extract-mediated protection against PD pathology. Altogether, apart from its potential as a functional food, the tested red seaweed, C. crispus, might find promising pharmaceutical applications for the development of potential novel anti-neurodegenerative drugs for humans.     

Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1)

Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%–70% dry matter) as compared to aqueous extraction (20%–25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC₅₀ of 77.6–126.8 μg/mL for CC and 36.5–41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID₅₀/cells without cytotoxity (1–200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID₅₀/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.     

Preliminary evaluation of anti-pyretic and anti-ulcerogenic activities of Sida cordifolia methanolic extract

The anti-pyretic and anti-ulcerogenic properties of methanolic extract of Sida cordifolia aerial parts (MESC) were investigated in rats. Oral dose of 500 mg/kg MESC significantly reduced pyrexia induced by TAB vaccine. MESC exhibited significant anti-ulcerogenic effect against aspirin and ethanol induced damage. Both these properties were comparable to the reference drugs.     

Human–wildlife conflict and attitude of local people towards conservation of wildlife in Chebera Churchura National Park,Ethiopia

Human–wildlife conflict is a serious challenge undermining the integrity of protected areas in developing countries. Developing effective human–wildlife conflict mitigation strategies requires an understanding of the conflict patterns, species involved and attitudes of local people living along protected area boundaries. We hypothesised that (1) there was a high level of human–wildlife conflict and (2) the local people would have less favourable attitudes towards problematic wild animals. We assessed patterns of human–wildlife conflict and attitudes of local people along the boundary of Chebera Churchura National Park, Ethiopia from 2012 to 2014. A total of 354 households were selected randomly for interview. A questionnaire survey, focus group discussions and direct field observations were carried out in the selected villages. The major types of human?wildlife conflict in the area include crop raiding, livestock predation, increased risk of livestock diseases and direct threats to human life. A majority of the respondents (68.1%) faced crop damage and domestic animal loss, 12.3% reported threat to humans and 0.3% reported that the wildlife might cause diseases. Close proximity of the villages to the park and seasons influenced livestock predation intensity with highest predation in the wet season (56.0%). To mitigate these problems, the local people utilised various traditional methods, including guarding. Most respondents had positive attitudes towards the conservation of wildlife. However, as the frequency of conflicts increased in the last five years, the attitudes of local people might change. Active measures are to be implemented to mitigate the problem and safeguard the future of the wildlife around the park. The park has enormous potential to benefit more local people by implementing a participatory management approach to conservation.     

Treatment of stephanofilariosis ('earsore') with ivermectin.

Thirty-four adult male buffaloes with clinical signs of 'earsore' were used to determine the efficacy of ivermectin against Stephanofilaria zaheeri. On the basis of parasite counts in skin scrapings, ivermectin was effective as a microfilaricide. Adult parasite counts were reduced, but removal of adults was incomplete. Clinical improvement was recorded in more than half of the treated buffaloes, but constant reinfection meant that meaningful interpretations were not possible.     

Assessment of impact of aquaculture on Kolleru Lake (India) using remote sensing and Geographical Information System

Aquaculture is one of the fastest growing food industries and the rapid growth of aquaculture worldwide has resulted in growing concerns about its impact on important ecosystems. Kolleru Lake, India's largest fresh water body, and Ramsar site have undergone tremendous changes due to the development of aquaculture. To assess the impact of aquaculture on Kolleru, satellite data were found appropriate because of the synoptic‐detailed overview and accuracy. Satellite data of IRS 1D, LISS III from 2004 and Survey of India topographic maps from 1967 were processed using image processing techniques in erdas imagine and analysed in Geographical Information System (GIS) such as arc gis 9.1. Land use map prepared from the satellite data was verified in the field using Global Positioning System to check the land and water use classes and its areal extent. It was estimated from the topographic maps of 1967 that the total lake boundary area was 180.38 km², in which 70.70 km² had water throughout the year and 100.97 km² had water during the rainy season. The digital image processing of 2004 satellite data revealed that Kolleru was no longer a lake and the lake area of 62.65 km² (34.73%) only remained in a degraded state, extensively colonized by macrophytes. The total loss of lake area was 109.02 km² between 1967 and 2004, in which aquaculture was developed in 99.74 km², which represented 55.3% of the 1967 lake area. The maximum conversion to aquaculture occurred from the lake liable to be inundated during the 1967 rainy season. The area under agriculture was 16.62 km² in 2004, the increase in lake area for agriculture between 1967 and 2004 being 8.22 km² (4.55% of lake). If human induced degradation is allowed to continue, the lake will very soon disappear. Now, the Government of Andhra Pradesh, India, has initiated schemes and laws to restore the lake to its pre‐development state. Lessons learnt from the Kolleru Lake cautioned that there is a need for regular monitoring of important water resources throughout the world to protect the biodiversity of the earth.     

Analysis of Seroreactivity against Cell Culture–Derived Bartonella spp. Antigens in Dogs

Background

Little is known about the specificity of Bartonella spp. immunofluorescent antibody (IFA) assays in dogs. Bacteremia in sick dogs most often has been associated with Bartonella henselae (Bh), Bartonella vinsonii subspecies berkhoffii (Bvb), and Bartonella koehlerae (Bk). Clarification of the diagnostic utility of IFA serology when testing against these organisms is needed.

Objective

To evaluate the specificity of Bartonella IFA assays utilizing 6 cell culture–grown antigen preparations.

Animals

Archived sera from SPF dogs (n = 29) and from dogs experimentally infected with Bvb (n = 10) and Bh (n = 3).

Methods

Antibodies (Abs) to Bvb genotypes I, II, and III, Bh serotype I, strains H‐1 and SA2, and to Bk were determined by IFA testing.

Results

Serum from naïve SPF dogs shown to be negative for Bartonella bacteremia did not react with any of the 6 Bartonella antigens by IFA testing. Dogs experimentally infected with Bvb genotype I developed Abs against homologous antigens, with no cross‐reactivity to heterologous Bvb genotypes, Bh H‐1, SA2 strains, or to Bk. Dogs experimentally infected with Bh serotype I developed Abs against Bh H‐1, but not to Bh SA2 strain with no cross‐reactive Abs to Bvb genotypes I–III or to Bk.

Conclusions and Clinical Importance

Bartonella spp. Ab responses during acute experimental infections are species and type specific.