Interaction of Rhizobium and Pseudomonas with Wheat (Triticum Aestivum L.) in Potted Soil with or Without P2O5

The effect of inoculation of Rhizobium or Pseudomonas, or both, in the presence and absence of phosphorus pentoxide (P₂O₅) fertilizer on wheat yield was tested. The experiment was conducted outdoors in potted soil during two consecutive years under natural conditions. Rhizobial strain (Thal 8-chickpea nodulating bacteria) and Pseudomonas strain (54RB), both indigenous P solubilizers were applied in broth culture at seedling stage. Fertilizer in the form of P₂O₅ was an additional treatment applied at sowing time. Results revealed that inoculation of Pseudomonas strain in presence of phosphorus (P) fertilizer increased yield up to 41.8% when it was compared with only P fertilizer applied. Co-inoculation of Rhizobium and Pseudomonas strains with the P₂O₅ treatment however resulted in increased grains yield by 10% over the P₂O₅ treatment alone. It is inferred that inoculation with Rhizobium and Pseudomonas combined with P₂O₅ is not only environmentally beneficial but also economically sound and productive, with wheat yield increases of 10% to 42%.     

Analysis of groundwater behaviour in a farmer controlled subsurface tile drainage system in Mardan, Pakistan

Extensive subsurface drainage system was installed in districtMardan in the North West Frontier Provinceof Pakistan in 1987 to control increasingwater logging and salinity problems due tocanal irrigation. Several recentlycompleted fields studies have indicatedthat subsurface drainage system hasenormously lowered watertable in certainareas due to extensive drainage network. Therefore, a study of controlled subsurfacedrainage technique was initiated in MardanScarp area to observe the temporal andspatial variations in water table depths ofthis specific case under various modes ofcanal irrigation and monsoon rains. Twoartificially drained areas, consisting of40 ha and 160 ha respectively, werecontrolled and selected for extensivemonitoring. A total of 98 observationswells (7.6 cm dia. and 4.1 m depth) wereinstalled in between lateral drains toobserve water table fluctuation. Theresults of this study are very interesting.Each of the two areas monitored in thestudy behaved differently. It was observedthat in one of the areas design water tabledepth at 1.1 m was maintained with properfunctioning of the controlled techniqueapplied to the subsurface drainage system. The results from this area showed that 25to 55% of the time throughout the yearachieved this objective whereas in thesecond area desired water table could notbe maintained and water table depth in thisarea remained between 2.0 to 2.7 m causingunnecessary water stress to plants. Alsoit was observed that watertable in theformer area is mostly controlled by thefunctional behavior of the irrigationcanal. In addition, the proper functioningof controlled techniques in subsurfacedrainage system supplemented veryefficiently to retain the groundwater levelto the optimal limits in dry season and tothe design ones in the others for timelyneeds of the crops. Also rainfalls havesignificant impact on the spatial andtemporal behaviors of water table depths inboth the areas during the monsoon season.     

Grafting-induced seed gossypol levels by demethylation of (+)-delta-cadinene synthase genes in upland cotton

Two-year-grafting experiments within and between Gossypium barbadense and Gossypium hirsutum accessions were conducted to study the source, transfer, and alteration of gossypol and methylation of (+)-δ-cadinene synthase (CDN) genes along with whole chromosomes of A01, A04, A11, D01, D05, and D11, harbouring the cotton CDNs, using the whole genome bisulfite sequencing (WGBS) method. The results revealed that although a glandless line used in this study had a low level of gossypol in the seeds, it did not contain any pigment glands, indicating that expression pathways of gland and gossypol were under genetic control of different loci in cotton. Grafts with glandless rootstocks produced significant amounts of total gossypol in the seeds, clearly demonstrating that the source of gossypol biosynthesis was not specific to the root. Heterografts consisting of scion GN, a glandless line, and scion TM-1 grafted on Pima 3-79 rootstocks caused increased total seed gossypol levels whereas homograft TT had decreased total seed gossypol levels. Alteration of total gossypol levels was also associated with gland density, and DNA demethylation levels of (+)-δ-cadinene synthase genes.     

Effects of dietary betaine on growth performance,fat deposition and serum lipids in broilers subjected to chronic heat stress

We evaluated the effects of supplementing betaine on growth performance, fat deposition and lipid metabolism status in broilers kept under chronic heat stress. Five hundred and forty chicks were randomly divided into six groups and the two normal temperature groups were held at normal ambient temperature and fed the basal diet (CONT) and basal diet plus 0.1% betaine, respectively. Heat stressed (HS) broilers were held at 32 ± 1°C from days 22 to 42 and fed the basal diet containing variable levels of betaine. Broilers were examined at days 28, 35 and 42 for body weight, feed consumption, fat deposition and serum lipids. The CONT and betaine‐supplemented groups showed higher (P < 0.01 or P < 0.05) feed consumption, body weight gain, and lower feed : gain ratio compared with the HS‐CONT group. Meanwhile, heat stress increased abdominal, intermuscular and subcutaneous fat deposition, whereas the supplemental betaine significantly decreased those compared with the HS‐CONT group. Additionally, betaine supplementation significantly decreased triglyceride, free fatty acids, low‐density lipoprotein cholesterol and high‐density lipoprotein cholesterol compared with HS‐CONT. Chronic HS reduces broiler production performance. However, betaine can reverse these negative effects partially and thus improve carcass composition by changing lipid metabolism.     

Mapping of QTL controlling tocopherol content in winter oilseed rape

Tocopherols are natural antioxidants in vegetable oils and are important dietary nutrients. Enhanced tocopherol content has become an important objective in oilseed rape breeding. A segregating DH population was tested for 2 years at two locations in replicated field trials. Genotypic differences occurred for α‐, γ‐ and total tocopherol content as well as α/γ‐tocopherol ratio, but highly significant genotype x environment interactions resulted in low heritabilities. Using a mixed‐model composite interval mapping approach between one and five QTL with additive and/or additive x environment interaction effects could be mapped for α‐, γ‐ and total tocopherol content and α/β‐tocopherol ratio. In addition, one to six locus pairs with epistatic interaction effects were identified, indicating a strong contribution of epistasis to trait variation. In total, the additive and epistatic effects explained between 28% (α‐tocopherol content) and 73% (total tocopherol content) of the genotypic variance in the population, with individual QTL and locus pairs contributing between 7.5 and 29.2% of variance. Considering the low heritabilities of the tocopherol traits, the results of this study indicate that marker‐assisted selection may be an efficient strategy in a breeding program for enhanced tocopherol content in rapeseed.     

Studies on Plant Population and Stand Establishment Techniques for Increasing Productivity of Rice in Dera Ismail Khan, Pakistan

Rice production in Pakistan is constraint by many factors pertaining to prevalent planting techniques. A research on the feasibility of new planting techniques (direct seeding on flat, transplanting on flat, direct seeding on ridges, transplanting on ridges and parachute planting) in transplanted and direct wet-seeded rice was undertaken at Dera Ismail Khan region of Pakistan’s North West Frontier Province during 2002 and 2003. Among the planting techniques, the best performance for the yield formation and economic evaluation was noted for transplanting on flat during both years. Chinese parachute planting technology also showed very promising results in most of the parameters. Direct seeding on ridges could not excel transplanting on flat and parachute planting during both cropping seasons. The findings concluded the feasibility of parachute planting technology along with traditional rice transplanting on flat over all other planting techniques being practiced in the area.     

Anti-inflammatory activities of Taxusabietane A isolated from Taxus wallichiana Zucc

Current study was conducted to identify constituents of Taxus wallichiana Zucc. that might be responsible for its folk use in anti-inflammatory conditions. Taxusabietane A was isolated from the bark extract of Taxus wallichiana Zucc. Taxusabietane A was analyzed for in-vitro and in-vivo anti-inflammatory activities using Lipoxygenase (LOX) inhibition assay and carrageenan-induced paw edema model. Taxusabietane A revealed considerable LOX inhibitory activity with the IC₅₀ value being 57 ± 0.31. Standard compound Baicalein showed the IC₅₀ value being 22.1 ± 0.03 μM. Taxusabietane A also showed significant (5 and 10 mg/kg) anti-inflammatory activity induced by carrageenan. However, this study highlighted the potential of Taxusabietane A to be further explored as a new lead compound for management of conditions associated with inflammation.     

Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs

In this study, we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed by SDS-PAGE. Fourteen bands ranging from 25 to 240 kDa in size were observed for cats, and 17 bands ranging from 25 to 289 kDa were observed for dogs. The densities of the bands differed among control, diabetic, and insulin-treated animals. In conclusion, we found that serum protein profiles and IGF-I concentrations were altered in both diabetic and insulin-treated animals. When judiciously interpreted in the light of other clinical and laboratory data, the techniques used in our study provide a valuable modality for measuring the severity of diabetes mellitus in dogs and cats.     

Marine Derived Hamacanthins as Lead for the Development of Novel PDGFRβ Protein Kinase Inhibitors

In this study, we report on pyrazin-2(1H)-ones as lead for the development of potent adenosine triphosphate (ATP) competitive protein kinase inhibitors with implications as anti-cancer drugs. Initially, we identified the pyrazin-2(1H)-one scaffold from hamacanthins (deep sea marine sponge alkaloids) by Molecular Modeling studies as core binding motif in the ATP pocket of receptor tyrosine kinases (RTK), which are validated drug targets for the treatment of various neoplastic diseases. Structure-based design studies on a human RTK member PDGFR (platelet-derived growth factor receptor) suggested a straight forward lead optimization strategy. Accordingly, we focused on a Medicinal Chemistry project to develop pyrazin-2(1H)-ones as optimized PDGFR binders. In order to reveal Structure-Activity-Relationships (SAR), we established a flexible synthetic route via microwave mediated ring closure to asymmetric 3,5-substituted pyrazin-2(1H)-ones and produced a set of novel compounds. Herein, we identified highly potent PDGFR binders with IC₅₀ values in an enzymatic assay below µM range, and possessing significant activity against PDGFR dependent cancer cells. Thus, marine hamacanthin-derived pyrazin-2(1H)-ones showing interesting properties as lead for their further development towards potent PDGFR-inhibitors.