排序方式： 共有88条查询结果，搜索用时 21 毫秒
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Samy METWALLY Rania HAMADA Alsagher O. ALI Hassan Y.A.H. MAHMOUD Nabil M. BAKER Adel E. A. MOHAMED Satoshi WADA Yasunobu MATSUMOTO Yoko AIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1676
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, the most common neoplastic disease of cattle worldwide and a serious problem for the cattle industry. Previous studies have shown the molecular prevalence of BLV and the coexistence of BLV genotype-1 and -4 in Egyptian dairy cattle; however, the molecular characteristics of BLV in Egyptian beef cattle are unknown. Therefore, we collected blood samples of 168 beef cattle from slaughterhouses in three governorates in Egypt. Based on BLV-CoCoMo-qPCR-2 targeting long terminal repeats and nested PCR targeting the env-gp51 gene, the BLV provirus infection rates were found to be 47/168 (28.0%) and 42/168 (25.0%), respectively. Phylogenetic analysis based on 501 bp of the BLV env-gp51 gene from 42 BLV isolates revealed that at least six distinctive strains (b, e, f, g, x, and z) were prevalent in cattle across the examined regions. Furthermore, phylogenetic analysis of the 420 bp sequence of the BLV env-gp51 region of the six strains against 11 known genotypes showed that the strains b, e, f, and g were clustered into genotype-1, and strains x and z were clustered into genotype-4. Our results also indicated that strains b and x exist in both dairy and beef cattle in Egypt. The present study is the first to detect and genotype BLV among beef cattle in Egypt. 相似文献
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Mitsuyo MATSUMOTO Takao MUKAI Satoru FURUKAWA Hitoshi OHORI 《Animal Science Journal》2005,76(5):507-512
Epigallocatechin gallate (EGCg) is the main active component of tea polyphenol and shows several biological activities, such as antimicrobial, antitumor‐promoting, anti‐inflammatory and anti‐oxidative activities. In the present study, the inhibitory effect of EGCg on bovine coronavirus (BCV) propagation in Madin‐Darby bovine kidney (MDBK) cells was investigated. EGCg at concentrations of less than 10 µg/mL did not show any cytotoxicity to MDBK cells. BCV propagation was significantly inhibited by pretreatment of the virus with EGCg (0.5–10 µg/mL) before virus inoculation in dose‐dependent, incubation time‐dependent and temperature‐dependent manners. The antiviral effect of pretreating MDBK cells with EGCg on BCV propagation was much weaker than that of pretreating BCV with EGCg. The hemagglutination activity of BCV was also reduced by EGCg in a dose‐dependent manner. These results demonstrate that EGCg possesses a distinct anti‐BCV activity and strongly suggest that EGCg interferes with the adsorption of BCV to MDBK cells by the interaction of EGCg with BCV particles. EGCg may therefore be a useful candidate for controlling BCV infection more effectively. 相似文献
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Makoto OTSUKA Aiko ISHIDA Yumi NAKAYAMA Mamoru SAITO Makoto YAMAZAKI Hitoshi MURAKAMI Yutaka NAKAMURA Mitsuto MATSUMOTO Katsuhiro MAMOTO Ryozo TAKADA 《Animal Science Journal》2004,75(3):225-229
Nondigestible oligosaccharides are not digested in the small intestine, but are fermented by bacteria colonizing in the large intestine. Physiological effects of non‐digestible oligosaccharides have been considered to be conferred by the fermentation of bacteria colonizing in the large intestine. Because cellooligosaccharide is a non‐digestible oligosaccharide, various physiological effects are expected. However, physiological functions of cellooligosaccharide are not well understood. This experiment was conducted to clarify the effect of dietary supplementation with cellooligosaccharide on the growth performance in weanling pigs. The result showed that average daily gain was significantly higher (P < 0.05) in pigs fed a diet supplemented with cellooligosaccharide than in pigs without cellooligosaccharide. There was a tendency to increasing average daily feed intake in pigs with cellooligosaccharide, though the significant difference was not detected (P = 0.18). Feed efficiency and nutrient digestibility of feces and ileum were not changed by feeding cellooligosaccharide. In addition, blood urea nitrogen was significantly higher (P < 0.05) in pigs fed the diet supplemented with cellooligosaccharide than in pigs without cellooligosaccharide. The concentrations of acetic and iso‐valeric acids in the cecum of pigs fed the diet with cellooligosaccharide tended to be higher (P < 0.10) than those without cellooligosaccharide. The results obtained in this study demonstrated that dietary supplementation with cellooligosaccharide improves growth performance in weanling pigs. 相似文献
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Ai GOTO Ryuichi KAMBAYASHI Hiroko IZUMI-NAKASEKO Yoshinori TAKEI Shinichi KAWAI Akio MATSUMOTO Keith G. LURIE Atsushi SUGIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):877
Motion of mitral valve during cardiac massage was examined using beagle dogs with ventricular fibrillation (n=4). Active compression-decompression cardiac massage (ACD-CM) exhibited greater peak aortic pressure than standard cardiac massage (S-CM), reverse of which was true for peak pulmonary capillary wedge pressure in each animal. Accordingly, peak aortic pressure was greater than peak pulmonary capillary wedge pressure with ACD-CM, whereas its reverse was true with S-CM. Transesophageal echocardiography revealed that mitral valve was incompletely closed with S-CM with showing regurgitation. The valve was more effectively closed during ACD-CM. These results indicate that effective closure of mitral valve during cardiac massage may increase forward blood flow, supporting “cardiac pump theory” rather than “thoracic pump theory” as a principle in dogs. 相似文献
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Yukari UETAKE Hitoshi NAKAMURA Masao ARAKAWA Ikuko OKABE Naoyuki MATSUMOTO 《Journal of General Plant Pathology》2001,67(3):175-181
A root box method with carrots was developed to estimate virulence of the violet root rot fungus, Helicobasidium mompa, to facilitate short-term screening of many isolates during a year. The root box consisted of two transparent acrylic plates
and a plastic bag of vermiculite in which two taproots of carrot were growing and inoculated with the fungus growing on fragments
of mulberry twigs. The boxes were kept in a greenhouse at 25°C, and the surface of carrots was observed weekly up to 14 weeks.
The virulence of each isolate was determined based on the number of weeks after inoculation required for the fungus to develop
infection cushions on the surface of carrots. Results were compared with those from the conventional inoculation method using
apple stocks. Two-year-old 456 apple stocks were planted with or without fungal inoculum in 30-cm diam. plastic pots containing
commercial soil and placed outdoors in April 1999. Symptoms on plant tops were observed weekly, and the first stocks were
killed 14 weeks after inoculation. At the end of trial 1 (6 months) and trial 2 (14 months), apple stocks were dug up to rate
disease index (DI) based on hyphal growth and infection cushion formation on the stem base. There was variability in disease
severity among replicates as well as isolate variability ; however, the results were similar in both trails. The level of
virulence estimated by both methods was almost parallel for a total of 23 isolates from five plant species, except for two
isolates from sweet potato that formed no obvious infection cushion on apple roots but on carrot were the most virulent.
Received 11 December 2000/ Accepted in revised form 23 March 2001 相似文献
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Masaki MAEZAWA Nao AKIYAMA Michihito TAGAWA Ken-ichi WATANABE Kotaro MATSUMOTO Hidefumi FURUOKA Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):819
A 2-year, 3-month-old Holstein cow presented with anorexia and enlarged superficial lymph nodes. Fine needle aspiration cytology of the superficial lymph nodes revealed large blast cells. Hematological examination revealed anemia, neutropenia, and blast cells in peripheral blood. Blast cells were the predominant cell type in bone marrow aspirates. Of the non-erythroid cells, 26%, 58%, and 18% were positive for myeloperoxidase, α-naphthyl acetate esterase, and naphthol AS-D chloroacetate esterase, respectively. Pathological examination revealed the proliferation of neoplastic cells, which were positive for monocytic markers, in the affected lymph nodes. The cow was diagnosed with acute myelomonocytic leukemia based on these findings. This report highlights the importance of performing bone marrow aspiration cytology and cytochemical staining when diagnosing bovine myeloid leukemia. 相似文献
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Naoyuki MATSUMOTO Akira KAWAKAMI Satoshi IZUTSU 《Journal of General Plant Pathology》2000,66(2):103-108
The distribution pattern and frequency of isolates of a snow mold fungus, Typhula ishikariensis biotype A belonging to a predominant MCG (mycelial compatibility group, referred to as super MCG), were surveyed throughout
its habitat from northern Honshu to eastern Hokkaido. About 38 and 14% of isolates examined belonged to super MCG in eastern
and central Hokkaido, respectively ; however, super MCG was never found in southern Hokkaido or northern Honshu. These findings
imply that T. ishikariensis biotype A consists of two populations in Japan, i.e., one that is distributed in Honshu and southern Hokkaido and lacks super MCG isolates, and the other that includes super
MCG isolates and exists in central and eastern Hokkaido. The difference in distribution pattern of the two populations is
discussed in terms of geological history during the Pleistocene (2 million to 10 thousand years ago). The tendency of global
warming, which alleviates freezing damage, was considered to be responsible for the outbreak of this fungus in eastern Hokkaido.
Received 6 December 1999/ Accepted in revised form 9 December 1999 相似文献
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Naoya MATSUMOTO Nanako SAWAYAMA Megumi ITOH Takahito TOYOTOME Kazutaka YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):368
Captive penguins with respiratory diseases exhibit advanced pathological conditions upon the appearance of clinical signs. Therefore, the successful treatment of respiratory diseases remains difficult after the onset of clinical signs, leading to high mortality rates. In this study, we measured air sac volume using computed tomography (CT) to evaluate the respiratory condition of penguins. In a regular quarterly health checkup, blood samples were collected from 45 penguins housed at an aquarium in Hokkaido, Japan. A total of 12 penguins with abnormal blood parameters underwent CT. The air sac volumes were calculated in three-dimensional CT, and the scatter plots of the air sac volumes and body weights were analyzed. No correlation was found between the air sac volume and body weight in both the gentoo and king penguins. Two gentoo penguins with infiltration and one king penguin with multiple nodules on CT were tentatively diagnosed with aspergillosis and treated with oral administration of itraconazole. Follow-up CT examination was performed until the outcome: healed or died. The mean air sac volumes of the two gentoo penguins, which recovered after treatment, increased from 273.9 and 329.0 cm3 before healing to 449.0 and 424.6 cm3 after healing, respectively. Meanwhile, the air sac volume of the king penguin, which subsequently died, decreased from 1,556.9 to 920.6 cm3 despite treatment. Changes of the air sac volume in the same individual could be useful for evaluating the respiratory condition of penguins. 相似文献