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排序方式: 共有452条查询结果,搜索用时 15 毫秒
91.
Miyawaki S Washizu M Maeda S Shibata S Watanabe K Yamazoe K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(9):1191-1193
Portal vein aneurysm (PVA) is a rare abnormal dilatation of the portal vein, which has not been reported in dogs. We describe the findings of ultrasound and computed tomography in a case of PVA in a young male toy poodle, with the final diagnosis established by explorative surgical observation. The dog had an aneurysmal fusiform dilatation in the extrahepatic portal vein with portal hypertension and multiple portsystemic shunts. This is the first report of canine PVA. 相似文献
92.
Chowdhury EH Kuribara H Hino A Sultana P Mikami O Shimada N Guruge KS Saito M Nakajima Y 《Journal of animal science》2003,81(10):2546-2551
Genetically modified corn has been approved as an animal feed in several countries, but information about the fate of genetically modified DNA and protein in vivo is insufficient. Genetically modified corn Bt11 is developed by inserting a recombinant DNA sequence encoding insecticidal Cry1Ab protein from Bacillus thuringiensis subsp. kurstaki. We examined the presence of corn intrinsic and recombinant cry1Ab gene by PCR, and the Cry1Ab protein by immunological tests in the gastrointestinal contents of five genetically modified corn Bt11-fed and five nongenetically modified corn-fed pigs. Fragments of corn zein (242 bp), invertase (226 bp) and of ribulose-1,5-bisphosphate carboxylase/ oxygenase genes (1,028 bp) were detected in the gastrointestinal contents of both Bt11 and nongenetically modified corn-fed pigs. Fragments of recombinant cry1Ab gene (110 bp and 437 bp) were detected in the gastrointestinal contents of the Bt11-fed pigs but not in the control pigs. Neither corn intrinsic nor cry1Ab gene fragments were detected in the peripheral blood by PCR. The gastrointestinal contents were positive for Cry1Ab protein by ELISA, immunochromatography, and immunoblot; however, these methods did not work for blood and precluded conclusions about any potential absorption of the protein. These results suggest that ingested corn DNA and Cry1Ab protein were not totally degraded in the gastrointestinal tract, as shown by their presence in a form detectable by PCR or immunological tests. 相似文献
93.
Mikami O Muneta Y Mori Y Yokomizo Y Nakajima Y 《Veterinary immunology and immunopathology》2002,90(3-4):203-207
Inflammatory cytokine mRNA expression in the lymphatic organs of neonatal, 1-month-old and adult pigs was compared. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18 and tumor necrosis factor (TNF)- in the spleen, thymus, tonsil and popliteal and mesenteric lymph nodes was investigated by semi-quantitative RT-PCR. Stronger IL-1β mRNA expression was observed in the 1-day-old and 1-month-old piglets than in the adult pigs. In thymus, tonsil and mesenteric lymph node, IL-1β mRNA expression in 1-day-old piglets was stronger than in 1-month-old pigs. The expression of IL-6 mRNA in the 1-day-old and 1-month-old tonsil tended to be stronger than in the adult pigs. IL-18 and TNF- mRNA expression was constant in all the samples examined. The expression of IL-1β and IL-6 mRNA may reflect an inflammatory reaction against the exo- and endogenous foreign bodies occurring in the lymphatic organs, especially in the tonsil, of neonatal piglets. 相似文献
94.
Takaaki Nishioka Koji Kitamura Toshio Fujita Minoru Nakajima 《Pesticide biochemistry and physiology》1976,6(4):320-337
The kinetic constants of a variety of substituted phenyl N-methyl- and N,N-dimethylcarbamates, which inhibit bovine erythrocyte acetylcholinesterase, were determined by various experimental procedures. A procedure in the presence of a chromogenic substrate was developed, based on the suggestion of Hart and O'Brien, and was compared with the conventional Main method. The dissociation equilibrium constant, Kd, and the carbamylation rate constant, k2, were shown to apparently depend on the inhibitor concentration range used for the determination in both procedures. Assuming that the binding of further inhibitor molecules to the reversible complex and the carbamylated enzyme is significant under conditions with high inhibitor concentrations, the concentration dependence of the kinetic constants was nicely delineated. It is indicated that reliable constants are obtainable with a rather low inhibitor concentration range, whose product by ki is of the order of 0.2–1.0 min?1. 相似文献
95.
Keiji Tanaka Norio Kurihara Minoru Nakajima 《Pesticide biochemistry and physiology》1976,6(4):392-399
In lindane-treated house flies, a cis-dehydrogenated metabolite, , was identified by gas-liquid chromatography and mass spectrometry. The in vitro metabolism study showed that in the presence of NADPH the microsomal fraction of house flies converted lindane to three hexane-soluble metabolites. This conversion was inhibited by piperonyl butoxide, SKF-525A, and carbon monoxide. These metabolites were identified as , (PCCHE) by gas-liquid chromatography. They, as well as lindane, were excellent substrates for the reaction with the postmicrosomal fraction in the presence of glutathione. While the reaction with lindane-d6 showed a significant deuterium isotope effect (6.82), that of did not (1.18). Enzymatic conjugation with glutathione probably occurs at the stage of PCCHE. 相似文献
96.
Takashi Kobayashi Eiji Kanda Shigeo Naito Toshihiko Nakajima Ichiro Arakawa Kazutoshi Nemoto Masanao Honma Hiroyuki Toujyou Kiyoshi Ishiguro Katsuki Kitada Yoichi Torigoe 《Journal of General Plant Pathology》2003,69(1):17-22
Rice reflectance was measured to determine the spectral regions most sensitive to leaf blast infection with a multispectral
radiometer. As disease severity increased, reflectance also increased in the 400–500 nm (blue), 570–700 nm (red), and 900–2000 nm
regions but decreased in the 500–570 nm and 700–900 nm regions. The increased reflectance in the blue and red regions may
be attributed to decreased chlorophyll and carotenoid contents in response to the blast infection. The maximum and minimum
reflectance differences occurred at 680 nm and 760 nm for the nondiseased and diseased rice, respectively. The spectral location
of maximum sensitivity was 675 nm regardless of disease severity. Rice reflectance ratios were evaluated as indicators of
leaf blast severity. Two ratios, R550/R675 (reflectance at 550 nm divided by reflectance at 675 nm), and R570/R675 quantified
the significant disease severity. These wavelengths were selected based on the sensitivity minima and maxima. The ratios of
nondiseased rice plants varied depending on growth stage. The variation in ratios must be considered when they are used to
estimate leaf blast severity.
Received: April 2, 2002 / Accepted: August 12, 2002 相似文献
97.
Moin?Uddin?Ahmad Yuri?Tashiro Shingo?Matsukawa Hiroo?OgawaEmail author 《Fisheries Science》2005,71(3):655-661
ABSTRACT: Horse mackerel and tilapia surimi were subjected to six different heat and pressure treatments in order to compare gelation characteristics of easy- and difficult-setting gels, that is, temperature dependence, by observing rheological properties and microscopic molecular mobility. The stress–relaxation and proton spin–spin relaxation time (1 H T 2 ) of water were measured for all treated gels. Horse mackerel gel demonstrated higher elasticity, large distribution of the stress–relaxation process, and smaller water 1 H T 2 than tilapia in both heat and pressure treatments. The water 1 H T 2 was steeply increased in the pressure treatment at around 294 MPa for both fishes. In contrast, the 1 H T 2 rarely changed in the heat treatment in spite of the considerable change in rheological properties. From the experimental results, it is considered that the gelation of horse mackerel (easy setting) surimi is induced by highly unfolding and re-aggregation of protein, which contributes to the formation of a strong network structure compared with tilapia in both heat and pressure treatments, and that pressure treatments hardly improve the gel strength of tilapia (difficult setting) surimi. The water 1 H T 2 measurement was used effectively in order to study gelation characteristics of easy- and difficult-setting fish through observing its molecular dynamics. 相似文献
98.
Takashi Kitagawa Aida Sartimbul Hideaki Nakata Shingo Kimura Harumi Yamada Akira Nitta 《Fisheries Science》2006,72(6):1166-1176
ABSTRACT: Immature Pacific bluefin tuna Thunnus orientalis , tagged with archival tags, were released near Tsushima Island in the East China Sea (ECS) during the winters of 1995, 1996 and 1997. Geolocations were estimated using the archival tags from recovered fish. These data, together with sea surface temperature (SST) data from satellite remote sensing, are used to describe the habitat used by these bluefin in the ECS from January to June for 3 years (1996, 1997, 1998), and to asses the effect of water temperature on fish distribution and movement. The results indicate that their geolocations ranged from the area north-east of Tsushima Island to the offshore area in the south-west. However, the area of highest density differed among years, being furthest south in 1996 and furthest north in 1998. The differences were probably caused by changes in SST associated with La Niña (1996) and El Niño (1998) events. Another densely populated area was identified in offshore waters of latitude 28–30 °N in 1996 (only), on the cold side of the Kuroshio front. These fish may have been prevented from moving northwards by an intrusion of Kuroshio water of approximately 25°C into the region immediately to the north-east. 相似文献
99.
100.
Nakajima Y Hoshi F Higuchi S Kawamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):343-345
An enzyme-linked immunosorbent assay was developed for the determination of canine beta2-microglobulin (beta2-m) in plasma and urine. The detectable sensitivity for pure canine beta2-m was 0.05 microg/l and the analytical range was 0.1 to 50 microg/l. The mean analytical recovery when pure canine beta2-m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1% in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of beta2-m in plasma and serum (n=17). The concentration of beta2-m in the plasma of normal dogs was 1.82 +/- 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 +/- 9.2 microg/l, 0.22 +/- 0.12 microg/kg of body weight or 14.2 +/- 9.4 microg/g of urine creatinine. The beta2-m creatinine index of random urine samples was 23.5 +/- 16.6 microg/g (n=26). There was a close correlation between the beta2-m creatinine index of 24 hr urine samples and that of random urine samples (r=0.872). 相似文献