The aim of the present study was to determine the chemical composition of Sambucus ebulus and Actinidia deliciosa ethanolic extracts as well as their in vitro antifungal activity on the mycelial growth of the water mold, Saprolegnia parasitica. The preliminary minimum inhibition concentration (MIC) and minimum lethal concentration (MLC) were determined by the HeMP method and finally, concentrations of each extract ranging from 1 to 10% were prepared by an agar dilution method to assess the in vitro antifungal activity, quantitatively. Both herbal extracts inhibited growth of Saprolegnia hyphae in vitro. Complete in vitro growth inhibition was found at a concentration of ≥5% for S. ebulus, whereas it was not observed even at 10% concentration of A. deliciosa. Based on gas chromatography coupled with mass spectrometry (GC/MS) analysis, the main constituents of S. ebulus include monophthalate (66.17%), fatty acids (26.47%), phytol (4.25%), and acetic acid (2.11%). Using colorimetric assays, A. deliciosa contained phenolic contents at 162 mg gallic acid (GAE)/g DW and flavonoid contents at 2.31 mg quercetin (QE)/g DW. In conclusion, the results of this study indicated that S. ebulus and A. deliciosa showed some antifungal activities against S. parasitica with formerly exhibiting stronger activity (p < 0.05).
Based on biochemical assays and electrophoretical methods, the inhibitory effects of three plant meals (soybean meal, wheat meal, winged bean meal) on digestive alkaline proteases of discus were investigated. Casein assays revealed that increasing levels of soybean meal caused a linear inhibitory effect on activity of protease. SDS-PAGE images revealed that trypsin and chymotrypsin were the inhibited enzymes. Soybean showed the lowest inhibition rate followed by wheat meal and raw winged bean. There was a quadratic relationship between wheat meal levels and its inhibition of protease acitivity. The highest inhibitory effect was obtained with the winged bean meal with inhibition of caseinolytic activities ranging from 3.6–98.6%. Results from this study showed the potential of both soybean meal and wheat meal as ingredients for practical diet for discus, while demonstrating the need for further improvement in processing method for winged bean meal. 相似文献
In order to use rice straw as forage in livestock feeding, the effects of steam-treated rice straw (at 15.5 kgf/cm2 for 120 s) feeding on growth performance, plasma volatile fatty acid profile, and nutrient digestibility of goats were determined. Twenty male goats (18.69?±?0.34 kg) were used in an 84-day trial. The goats were divided into four groups of five goats each to receive steam-treated (STRS) or untreated (UTRS) rice straw diet under closed house (CH) and open house (OH) systems. The results revealed that the goats fed with STRS had significantly higher dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility; similarly, the average daily weight gain and feed conversion ratio were higher for STRS groups under both CH and OH systems than those for UTRS. The plasma protein and insulin in STRS and cholesterol in UTRS groups was higher (P?0.05) at 60 days but found not different (P?>?0.05) at 30 days. The plasma amylase, lipase, T3, T4 and glucagon at 30 and 60 days were not different (P?>?0.05) among the groups. The plasma acetate, propionate, butyrate, and total volatile fatty acid were higher (P?0.05) in STRS groups at 30 and 60 days. The housing conditions had no effects (P?>?0.05) on these parameters. It could be concluded that steam treatment of rice straw at 15.5 kgf/cm2 for 120 s increased apparent nutrient digestibility, hence increased the growth and feed efficiency of growing goats. 相似文献
Studies of ocular development in the dromedary camel (Camelus dromedarius) have not been reported previously. The aim of the present investigation was therefore to document the major landmarks and the time course in the prenatal development of the eye tunics in dromedary camel and its accommodation with the surrounding hard environment of the desert. Serial histological sections of dromedary camel embryos and foetuses were used. Age estimation was made on the basis of gestational size, crown vertebral‐rump length (CVRL), which ranged 1.2–110 cm. The eye of the dromedary camel developed in a similar manner to that of the human and domestic animals eyes; the principal differences were in the time of occurrence of certain developmental events, pigmented peripheral cornea near the limbus, a remarkably thickened Descemet's membrane and pigmentation in the corneo‐scleral junction, which represent an adaptive modification in relation to a severe environment. 相似文献
Zagros forests are mainly covered byQuercus brantii L. coppices and oak sprout clumps occupy the forest area like patches. We investigated post-fire herbaceous diversity in the first growing season after fire. For this purpose neighboring burned and unburned areas were selected with the same plant species and ecological conditions. The data were collected from areas subjected to different fire severities. Overall 6 treatments were considered with respect to fire severity and the mi-crosites of inside and outside of oak sprout clumps including: unburned inside and outside of sprout clumps (Ni and No), inside of sprout clumps that burned with high fire severity (H), inside of sprout clumps that burned with moderate fire severity (M), outside of sprout clumps that burned with low fire severity (OH and OM). Different herbaceous com-position was observed in the unburned inside and outside of oak sprout clumps. The species diversity and richness were increased in treatments burned with low and moderate fire severity. However, in treatment burned with high fire severity (H), herbaceous cover was reduced, even-ness was increased, and richness and diversity were not significantly changed. We concluded that besides the microsites conditions in forest, fire severity is an inseparable part of the ecological effect of fire on her-baceous composition. 相似文献
Temperature (high and low) is one of the most limiting environmental factors for growth and production of fruits in different parts of the world. For temperate-zone fruit species, a certain total heat requirements are needed in order to obtain ripe fruits. The aim of this study was to determine heat requirements of three commercial pomegranate cultivars (‘Suruc’, ‘Katirbasi’ and ‘Hicaznar’) grown in Southern Anatolia region. The cultivars showed a narrower range of heat requirements for flowering (growing degree hours, GDH), i.e. ‘Suruc’ (25,000), ‘Katirbasi’ (25,270), ‘Hicaznar’ (28,000) and a wide range of heat requirements for fruit ripening, i.e. ‘Suruc’ (73,670), ‘Katirbasi’ (74,105) and ‘Hicaznar’ (88,052) GDH. Under the Sanliurfa province conditions, two years average of effective heat summation requirement from bud swelling to 50 % flowering stage for ‘Suruc’, ‘Katirbasi’ and ‘Hicaznar’ cultivars are calculated as 643, 655 and 718 growing degree days (GDD) and from bud swelling to harvest are 2734, 2802 and 3289 GDD. It can be concluded that the effective heat summation of Sanliurfa province is enough for commercial growing for all pomegranate cultivars. 相似文献
The accumulation of inbreeding and the loss of genetic diversity is a potential problem in Holstein dairy cattle. The goal of this study was to estimate inbreeding levels and other measures of genetic diversity, using pedigree information from Iranian Holstein cattle. Edited pedigree included 1 048 572 animals. The average number of discrete generation equivalents and pedigree completeness index reached 13.4 and 90%, respectively. The rate of inbreeding was 0.3% per year. Effective number of founders, founder genomes, non‐founders and ancestors of animals born between 2003 and 2011 were 503, 15.6, 16.1 and 25.7, respectively. It was proven that the unequal founder contributions as well as bottlenecks and genetic drift were important reasons for the loss of genetic diversity in the population. The top 10 ancestors with the highest marginal genetic contributions to animals born between 2003 and 2011 and with the highest contributions to inbreeding were 48.20% and 63.94%, respectively. Analyses revealed that the most important cause of genetic diversity loss was genetic drift accumulated over non‐founder generations, which occurred due to small effective population size. Therefore, it seems that managing selection and mating decisions are controlling future co‐ancestry and inbreeding, which would lead to better handling of the effective population size. 相似文献
The temporal dynamics of appetite (weight of food consumed per day) were analysed for fish fed to satiation after a 1 or 2-week period of feed deprivation. Three species were compared: two omnivores (minnow, Phoxinus phoxinus and gibel carp, Carassius auratus gibelio) and a carnivore (three-spined stickleback, Gasterosteus aculeatus). All species showed compensatory changes in appetite and growth during the re-feeding period but in the stickleback, there was a lag of a week before the compensatory response was detected. The temporal dynamics of appetite differed between the three species but not within a species. Appetite of the minnow declined towards control levels from the onset of re-feeding. In gibel carp, appetite increased to a peak and then declined to control levels. In the stickleback, appetite was initially below control levels, increased to a maximum, then declined towards control levels. The differences between the species might have been artefacts of the experimental protocols used but could also reflect underlying differences in the control of appetite in these species of fish. 相似文献
Low level of iron in staple food crops is one reason for the predominance of iron-deficiency anemia in developing countries. Most of the iron in rice grains accumulates in the outer aleurone layer and embryo, which are removed during milling, and the edible endosperm contains very low amounts of iron. In an effort to increase iron nutrition, we report here the transgene introgression of a high-iron trait into a high-yielding indica rice cultivar. The ferritin gene from soybean (soyfer1) was introduced into rice plants through interbreeding between soybean ferritin-overexpressing transgenic IR68144 and the high-yielding cultivar Swarna. The stable integration of the soyfer1 gene was confirmed in the BC2F4 generation, and the hybrid seeds showed 2.6-fold soybean ferritin gene expression over the recurrent parent Swarna. The hybrid milled seeds revealed a 2.54-fold increase in iron and 1.54-fold increase in zinc compared to Swarna. Agronomic data and an SSR marker analysis of the hybrid rice plants were taken into account for NIL character identification. 相似文献