全文获取类型
收费全文 | 1145篇 |
免费 | 66篇 |
国内免费 | 10篇 |
专业分类
林业 | 77篇 |
农学 | 81篇 |
基础科学 | 27篇 |
420篇 | |
综合类 | 102篇 |
农作物 | 108篇 |
水产渔业 | 66篇 |
畜牧兽医 | 202篇 |
园艺 | 29篇 |
植物保护 | 109篇 |
出版年
2023年 | 16篇 |
2022年 | 50篇 |
2021年 | 73篇 |
2020年 | 73篇 |
2019年 | 94篇 |
2018年 | 115篇 |
2017年 | 105篇 |
2016年 | 80篇 |
2015年 | 40篇 |
2014年 | 50篇 |
2013年 | 134篇 |
2012年 | 61篇 |
2011年 | 74篇 |
2010年 | 47篇 |
2009年 | 31篇 |
2008年 | 39篇 |
2007年 | 28篇 |
2006年 | 25篇 |
2005年 | 11篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 1篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有1221条查询结果,搜索用时 293 毫秒
101.
Muhammad Youssef Arif Saeed Alhammadi Jorge Humberto Ramírez-Prado Lorenzo Felipe Sánchez-Teyer Rosa María Escobedo-GraciaMedrano 《Genetic Resources and Crop Evolution》2018,65(2):577-590
Here, two Punica species, viz., P. protopunica Balf. fil., reported as native to Socotra, and P. granatum L., were compared for the first time. Analysis of one P. protopunica and eleven P. granatum accessions was performed using three molecular markers, i.e., sequence related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), and intron targeted amplified polymorphism (ITAP), along with analysis of pgWD40 sequences, a gene involved in anthocyanin biosynthesis. All markers revealed the relationship between the two species and placed them at 33% similarity. SRAP, TRAP, and ITAP generated a total of 299, 260, and 160 bands, respectively. Of these, 78, 74, and 41 bands were specific for P. protopunica, and 92, 85, and 57 bands, respectively, were shared between both species. Sequence analysis of pgWD40~870 bp amplicons exhibited 100% identity among P. granatum accessions and 98% identity to that of P. protopunica. Phylogenetic analysis of WD40 sequences from monocot and dicot species, including both Punica species confirmed the relation between P. protopunica and P. granatum, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. Furthermore, the genetic diversity among and within P. granatum accessions from Egypt (3), Mexico (5), and Yemen (3) was assessed. Molecular marker-based relationships among region-bulked accessions was approximately the same (~90% similarity), whereas the degree of genetic variation was altered within each region. Specific bands (alleles) for accessions of each region along with those shared among them were identified. Thus, these bands could be used for pomegranate genotyping and breeding programs. 相似文献
102.
Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers 下载免费PDF全文
Matthew S. Rodda Shimna Sudheesh Muhammad Javid Dianne Noy Annathurai Gnanasambandam Anthony T. Slater Garry M. Rosewarne Sukhjiwan Kaur 《Plant Breeding》2018,137(4):492-501
This study describes the identification of a quantitative trait locus (QTL) in the recombinant inbred line population of ILL2024 × ILL6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes. 相似文献
103.
Muhammad Amir Maqbool Muhammad Aslam Abdurahman Beshir Muhammad Sarwar Khan 《Plant Breeding》2018,137(4):451-469
Vitamin A deficiency is widely prevailing in children and women of developing countries. Deficiency of vitamin A causes night blindness, growth retardation, xerophthalmia and increases the susceptibility against epidemic diseases. Among different interventions of overcoming malnutrition, biofortification is the most acceptable and preferred intervention among researchers, growers and consumers. Maize is grown and consumed in those regions where vitamin A deficiency is most prevalent; thus, targeting this crop for provitamin A biofortification is the most appropriate solution. Different breeding strategies including diversity analysis, introduction and stability analysis of exotic germplasm, hybridization, heterosis breeding, mutagenesis and marker‐assisted selection are practised for exploring maize germplasm and development of provitamin A‐enriched cultivars. Genome‐wide association selection and development of transgenic maize genotypes are also being practised, whereas RNA interference and genome editing tools could also be used as potential strategies for provitamin A biofortification of maize genotypes. The use of these breeding strategies for provitamin A biofortification of maize is comprehensively reviewed to provide a working outline for maize breeders. 相似文献
104.
Asma Saleem Iftikhar Fareed Qaisar Mahmood A. Egrinya Eneji Muhammad Shahzad 《Compost science & utilization》2018,26(2):114-127
Poultry litter (PL) is a significant source of nutrients, but a suitable amount of carbon needs to be added as a bulking agent during its composting to a stable nutrient source. Here, we characterized the transformation of phosphorus and other plant nutrients during aerobic composting of PL with sugarcane (SW) and cabbage waste (CW) for 120 d. Periodic samples were collected during composting and analyzed for total C, P (and its fractions), K, Ca, Mg, Cu, Fe, Zn, Mn, EC and pH. At the initial stage of composting (20 d), the P fractions varied in the order: water soluble P > NaHCO3-P (readily plant-available) > HCl-P (Ca+Mg-bound) > residual-P > NaOH-P (Fe+Al-bound). After 120 d, the order was HCl-P > residual-P > water-P > NaHCO3-P > NaOH-P. These variations suggested a transformation of labile Pi to more recalcitrant forms during composting. Water soluble P was the dominant fraction during the initial composting period. This declined progressively with time of composting, while the HCl-P increased, confirming the transformation of the more vulnerable water soluble P to the more recalcitrant HCl-extractable P. This indicated that the composting could be a useful way of managing manure for P stabilization and reducing its losses in runoff water following land application. The total C varied directly with the ratio of sugarcane and cabbage wastes in the manure but was inversely related to the duration of composting. Extractable K, Ca, Mg, and Na increased whereas trace elements concentrations decreased with time of composting. The higher availability of basic plant nutrients and reduced availability of heavy metals in the mature compost are valuable attributes for safe use in sustainable agricultural production. 相似文献
105.
Chuan-qi XIA Aziz-Ur-Rahman Muhammad Wen-jing NIU Tao-qi SHAO Qing-hua QIU Huawei SU Bing-hai CAO 《农业科学学报》2018,17(2):415-427
Twenty-eight male, weaned Chinese Holstein calves((156.8±33.4) kg) were used to investigate the effects of dietary forage to concentrate ratio(F:C) and forage length on nutrient digestibility, plasma metabolites, ruminal fermentation, and fecal microflora. Animals were randomly allocated to four treatments in a 2×2 factorial arrangement: whole-length forage(WL) with low F:C(50:50); WL with high F:C(65:35); short-length forage(SL) with high F:C(65:35); and SL with low F:C(50:50). Chinese wildrye was used as the only forage source in this trial. The grass in the SL treatments was chopped using a chaff cutter to achieve small particle size(~50% particles 19 mm). Dry matter intake(DMI) and organic matter(OM) intake was increased by increasing both F:C(P0.01) and forage length(FL)(P0.05), while acid detergent fiber(ADF) and neutral detergent fiber(NDF) intakes were only increased by increasing the F:C(P0.01). The digestibility of NDF was increased as the FL increased(P0.01), and it was also affected by interaction between F:C and FL(P0.05). Cholesterol(CHO)(P0.01), leptin(LP)(P0.05), and growth hormone(GH)(P0.01) concentrations in plasma were increased as dietary F:C increased. A significant increase in plasma triglyceride(TG)(P0.01), insulin(INS)(P0.05), and GH(P0.01) levels was observed with decreasing dietary FL. Ruminal p H values of calves fed with low F:C diets were significantly lower than those in high F:C treatment(P0.05). Increasing the F:C enhanced ruminal acetic acid(P0.05) and acetic acid/propionic acid(P0.01). Fecal Lactobacillus content was significantly higher, while Escherichia coli and Salmonella contents were significantly lower in WL and high F:C groups(P0.05). Lower fecal scores(higher diarrhea rate) were observed in calves fed with SL hay compared to WL hay(P0.05). Denatured gradient gel electrophoresis(DGGE) bands and richness index(S) were significantly affected by the interaction between F:C and FL(P0.05), under high F:C, band numbers and richness index from WL group were higher than that from SL group(P0.05), whereas there were no differences between WL andSL groups under low F:C(P0.05). Microflora similarity was 50–73% among the different treatments. It is concluded that the WL with high F:C(65:35) diet is suitable for weaned calves. 相似文献
106.
地下LoRa无线传感器网络的传输测试系统研究 总被引:1,自引:1,他引:0
【目的】以智能手机为核心,研制成本低、使用简单的面向LoRa地下无线数据传输的测试系统。【方法】该测试系统由安装了测试用的App智能手机和与之匹配的地下测试节点组成。利用该系统进行场景测试,测定影响土壤中LoRa无线数据传输效果的因素并讨论对应的数据传输方法。【结果】测试结果表明,该系统运行可靠、使用方便灵活,LoRa低功耗广域网技术亦可以较为可靠地满足土壤介质中的无线传感器网络数据传输需求。【结论】本系统可为未来地下LoRa无线传感器网络的工程应用提供相应的测试手段。 相似文献
107.
Saqib Bashir Muhammad Shaaban Qaiser Hussain Sajid Mehmood Jun Zhu Qingling Fu Omar Aziz Hongqing Hu 《Journal of Soils and Sediments》2018,18(9):2948-2959
Purpose
Soil contamination with heavy metals, such as Cd and Pb, has caused severe health and environmental risks all over the world. Possible eco-friendly solutions for Cd and Pb immobilization were required to reduce its mobility through various cost-effective amendments.Materials and methods
A laboratory incubation study was conducted to assess the efficiency of biochar (BC), zeolite (ZE), and rock phosphate (RP) as passivators for the stabilization of Cd and Pb in paddy soil as well as soil microbial biomass. Various extraction techniques were carried out: a sequential extraction procedure, the European Community Bureau of Reference (BCR), toxicity characteristic leaching procedure (TCLP) test, and single extraction with CaCl2. The impact of passivators on soil pH, dissolved organic carbon (DOC), and microbial biomass (carbon, nitrogen, and phosphorus) was examined in the metal contaminated soil.Results and discussion
The results showed that the exchangeable portion of Cd in soil was significantly reduced by 34.8, 21.6, and 18.8% with ZE, RP, and BC at a 3% application rate, respectively. A similar tendency of reduction in Pb soluble portion was observed by ZE (9.6%), RP (20%), and BC (21.4%) at a 3% application rate. Moreover, the TCLP leachate of Cd and Pb was apparently reduced by 17 and 30.3% with BC at a 3% application dose, respectively, when compared to the control. Soil pH, nutrients, and microbial biomass C, N, and P were significantly increased with the addition of BC, RP, and ZE passivators.Conclusions
The results showed that the incorporation of BC, ZE, and RP significantly reduced the Cd and Pb mobility in paddy soil as well as enhanced soil nutrients and microbial biomass. Overall, among all the amendments, rice straw derived-BC performed better for Cd and Pb immobilization in paddy soil.108.
Rosmawati Effendi Abustam Abu Bakar Tawali Muhammad Irfan Said Dwi Kesuma Sari 《Fisheries Science》2018,84(6):1081-1089
Snakehead fish (Channa striata) skin is a fishery by-product that has the potential for further processing because it contains a high amount of organic matter. This study investigates the effect of body weight on the chemical composition and collagen content of snakehead fish skin. This study used fresh snakehead fish of either gender. Their body weights were divided into three groups: small, medium, and large size. The characteristics of snakehead fish skin included proximate composition, amino acid content, collagen content, microstructure, and minerals. Snakehead fish skin from fish of different body weights indicated that the moisture content and ash tended to decrease, the protein content was relatively stable, and the lipid content tended to increase with increasing body weight. Glycine and proline comprised the highest percentages of amino acids at all levels of body weight, and the presence of hydroxyproline showed that snakehead fish skin was the source of collagen. There was no significant difference in body weight observed on collagen protein content of the skin. This study on the microstructure and mineral content of snakehead fish skin can be used as supporting information to promote the potential utility and economic value of the skin. 相似文献
109.
Rana M. Sabir Tariq Khalid P. Akhtar Amjad Hameed Najeeb Ullah Muhammad Y. Saleem Imran ul Haq 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):911-922
Shoe-string disease caused by Cucumber mosaic virus (CMV) is one of the major threats to tomato production worldwide. The alteration in some biochemical parameters in leaves of the susceptible tomato genotype (Nagina) associated with CMV infection and the effect of exogenous application of salicylic acid (SA) and benzothiadiazole (BTH) were studied in this paper. Results showed that exogenous treatment with SA and BTH not only led to plants which gave significantly more yield than diseased controls (DC), but also delayed symptom expression and reduced disease severity. Analysis of biochemical parameters indicated that exogenous application of elicitors and viral infection, significantly affected the activity of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Compared to the DC plants, minimum disease severity and maximum number of fruit were recorded after a single dose of SA + BTH. Maximum plant height was recorded after weekly application of SA and maximum fruit yield per plant was gained with single dose of SA. Moreover, the activity of POD was significantly elicited many-fold after weekly application of SA + BTH, while higher amount of SOD was recorded with single dose of SA. The activity of CAT was also significantly accelerated after weekly application of SA + BTH while increased level of APX was noticed with single dose of BTH. In conclusion, the combined application of SA and BTH played an important role in induction of defense mechanism against CMV infection and can be useful in tomato disease management programs. 相似文献
110.
Bioefficacy of dietary chromium picolinate and chromium yeast on growth performance and blood biochemical in Red Tilapia,Oreochromis niloticus (Linnaeus) 下载免费PDF全文