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961.
Rafiq Ahmad Mahmood-ul- Hassan Ghanan B. Akhtar Sadia Saeed Sabaz A. Khan Muhammad Kausar Nawaz Shah Nadeem Khan 《Plant Breeding》2020,139(5):988-995
Onion is one of the major vegetable crops in terms of production as well as consumption. In the current research, available onion genetic stock was evaluated to identify male-sterile lines and produce high-yielding F1 hybrids for future breeding programmes. A mitochondrial DNA-based marker was mapped and correlated with phenotypic traits to isolate male-sterile plants. Based on the floral and pollen structure, nine putative male-sterile lines were identified. On the other hand, for nuclear marker identification at Ms locus, two sets of primers were used, one for Ms dominant allele and another for sterile and maintainer plants. Results revealed that 70% of open pollinated varieties (OPVs) possess plants with sterile cytoplasm coupled with genetic sterility at Ms locus, called sterile “A” line. Approximately 20% of plants in some genotypes were identified with normal (N) cytoplasm having recessive fertility gene at Ms locus, called maintainer “B” line. Based on the present findings, “A”, “B” and “R” (restorer line), future F1 hybrid seed production systems in onion is discussed. 相似文献
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Muhammad I Illijas Masaru Terasaki Ryo Nakamura Noriaki Iijima Akihiko Hara Nobuhiro Fusetani Yutaka Itabashi 《Fisheries Science》2008,74(3):670-676
ABSTRACT: A glycerolipid acyl-hydrolase was purified 19-fold with a yield of 11% from the prostaglandin-producing red alga Gracilaria vermiculophylla by ammonium sulfate precipitation, anion-exchange chromatoraphy and gel filtration chromatography. Sodium dodecylsulfate– polyacrylamide gel electrophoresis of the final preparation showed a single band corresponding to a molecular mass of 20 kDa, but Superdex 200 fast protein liquid chromatography exhibited a molecular mass of 40 kDa. Accordingly, it was suggested that the purified enzyme was a homodimer of a 20 kDa subunit. The optimal temperature and pH were 37°C and 7–8, respectively. The purified enzyme catalyzed hydrolysis of the acyl groups of both glycoglycerolipids and phospholipids, especially monogalactosyldiacylglycerol and phosphatidylcholine. These results suggest that the enzyme hydrolyze the membrane lipids of the alga to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis. 相似文献
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Aliza D Ismail IS Kuah MK Shu-Chien AC Tengku Muhammad TS 《Fish physiology and biochemistry》2008,34(2):129-138
Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining
the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible
gene in swordtail fish, Xiphophorus helleri. Using ACP-based RT-PCR coupled with RLM-RACE, we cloned Wap65, a mammalian homologue of hemopexin gene. The gene exhibits
high identity at amino acid levels with the Wap65 gene of other fish species (42–68%) and mammalian hemopexin gene (35–37%).
In addition, ten cysteine and two histidine residues are conserved in the swordtail fish Wap65 gene. These cysteine residues
are vital for structural integrity, and histidine residues provide high binding affinity towards heme. As revealed by RT-PCR,
the gene was upregulated in swordtail fish that were exposed to copper in a dose- and time-dependent manner. Therefore, the
identification of Wap65, a mammalian homologue of hemopexin, as a new copper-inducible gene will provide greater insight into
the role of this gene in copper metabolism. 相似文献
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Muhammad A. I. Dalia A. M. Loh T. C. Akit H. Samsudin Anjas A. 《Veterinary research communications》2022,46(2):431-445
Veterinary Research Communications - This study compares the effects of sodium selenite, selenium yeast, and enriched bacterial organic selenium protein on antioxidant enzyme activity, serum... 相似文献