The androgenic gland and monosex culture of freshwater prawn Macrobrachium rosenbergii (De Man): a biotechnological perspective

Males of the freshwater prawn Macrobrachium rosenbergii (De Man) grow faster and reach a larger size at harvest than females of the species. It is thus obvious that culture of monosex all‐male populations would be economically advantageous. Sexual differentiation in crustaceans is regulated by the androgenic gland (AG), which plays a pivotal role in the regulation of male differentiation and in the inhibition of female differentiation. In M. rosenbergii, AG removal from immature males resulted in sex reversal, with complete female differentiation. Similarly, AG implantations into immature females lead to the development of the male reproductive system. Sex‐reversed M. rosenbergii animals were capable of mating with normal specimens to produce offspring. Early attempts in Israel and more recently, attempts in other countries to establish all‐male populations through manual segregation showed that for the production of monosex prawn populations to be economically feasible, intervention via the AG is probably required. However, a suitable biotechnology is still to be developed, and an androgenic hormone has yet to be identified in decapods. Three lines of aquacultural and biotechnological research and development are proposed for the future: (1) Establishment of monosex cultures through manual segregation, together with the application of selective harvesting and claw ablation, as well as examination of different monosex culture strategies under a variety of economic conditions. (2) Microsurgical intervention in the AG, leading to the development of functional neo‐females, which would subsequently be mated with normal males to produce all‐male progeny. (3) Elucidation of AG bioactive products to enable biochemical or molecular manipulation of sex differentiation.     

Contribution of nitrogen fixed by Azospirillum to the n nutrition of spring wheat in Israel

Acetylene reduction activity (ARA) was measured in cores containing roots of various Israeli wild and cultivated wheat lines colonized by Azospirillum. The inoculated plants were grown under greenhouse or field conditions. Although, no measurable ARA was detected during earlier stages of wheat development, 50–600 nmol C₂H₄ g^?1 dry root h^?1 was measured during heading and flowering stages. By using N yield balance and ¹⁵N dilution techniques, it was found that Triticum aestivum cv. Miriam inoculated with Azospirillum accumulated 20% more N (¹⁴N and ¹⁵N) at the booting stage than did the uninoculated control. This difference in N content became less apparent in grains. No significant ¹⁵N dilution could be found and the contribution of atmospheric N₂ to the N content of grains of inoculated plants was negligible. It was concluded that the potential contribution of biological N₂ fixation to spring wheat cultivation in Israel is very low.     

Effect of seawater concentration on the productivity and nutritional value of annual Salicornia and perennial Sarcocornia halophytes as leafy vegetable crops

The halophyte Salicornia was recently introduced as a fresh vegetable crop that thrives in extreme salt conditions. Two annual Salicornia and two perennial Sarcocornia ecotypes were investigated for yield production and nutritional value in response to different seawater concentrations in the irrigation solution. A harvest schedule based on a three-week cycle gave better productivity than a two-week or a four-week cycle. Total yield declined with increasing percentage of seawater above 50% in the irrigation water, however annual plants had always ca 2–3-fold higher fresh biomass in comparison to their perennial counterparts. Increased percentages of seawater in the irrigation solution had the following effects on ion concentrations in the shoots: no change in Ca²⁺ and Mg²⁺, a slight increase in K⁺, and marked elevations in Na⁺ and Cl⁻. Importantly, total polyphenol, β-carotene and ureides, all known for their antioxidant capacities, rose with increasing seawater percentage, findings that indicated improved nutritional values for Salicornia and Sarcocornia irrigated with high concentrations of seawater. Impressively, both the annual Salicornia and the perennial Sarcocornia ecotypes exhibited high total shoot lipid contents of up to 2.41 and 2.06 mg g⁻¹ fresh weight, respectively, which included an omega-3 fraction of 47.6 and 41.2% of the total fatty acid content. Moreover, the high fatty acid content of the annual Salicornia ecotype was not significantly affected by increasing seawater concentrations. In this study, we thus demonstrated the feasibility of cultivating Salicornia and Sarcocornia by applying a multiple harvest system and 100% percentages of seawater in the irrigation water generating economic yields with high nutritional value. The findings also showed that Salicornia and Sarcocornia leafy vegetables may attract additional interest as an alternative source of omega-3 polyunsaturated fatty acids for human consumption, even when the crop irrigated solely with seawater.     

贝莱斯芽胞杆菌B006对不同水肥条件下娃娃菜生长及软腐病防效的影响

贝莱斯芽胞杆菌B006是一株高效生防菌株,但其在不同水肥条件下的防病促生效果尚不明确.本研究通过盆栽试验测定了不同土壤含水量和施肥条件下B006菌剂对娃娃菜生长及对软腐病防效的影响,结果表明:在土壤含水量为14％、施肥量为3.57 g/kg土的条件下,播种时沟施5 g B006菌剂(菌含量为1×107 CFU/g),出...     

Plant growth parameter estimation from sparse 3D reconstruction based on highly-textured feature points

Crop canopy spatial parameters are indicative of plant phenological growth stage and physiological condition, and their estimation is therefore of great interest for modeling and precision agriculture practices. Rapid increases in computing power have made stereovision models an attractive alternative to common single-image-based 2D methods, by allowing detailed estimation of the plant’s growth parameters regardless of imaging conditions. Models that have been proposed thus far are still limited in their application because of sensitivity to outdoor illumination conditions and the inherent difficulty in modeling complex plant shapes using only radiometric information. Assuming that not all of the plant-related pixels are essential for growth estimation, this study proposes a 3D reconstruction model that focuses on selected salient features on the plant surface, which are sufficient for obtaining growth characteristics. In addition, by introducing a hue-invariant model, the proposed algorithm shows robustness to diverse outdoor illumination conditions. The algorithm was tested under greenhouse and field conditions on corn, cotton, sunflower, tomato and black nightshade plants, from young seedlings to fully developed plant growth stages, and accurately estimated height (error ~4.5 %) and leaf cover area (error ~5 %). Furthermore, a strong correlation (r² ~0.92) was found between the plant’s estimated volume and measured biomass, yielding an accurate biomass estimator in the validation tests (error ~4.5 %). This estimation ability remained stable while applying the model on plants with varying densities (overlapping leaves) and imaging setups where the standard 2D based analyses failed, thus showing the 3D modeling contribution to robust growth estimation models.     

Paleosols of the southern coastal plain of Israel

Paleosols of the S coastal plain of Israel were studied in a characteristic sequence situated in the Ruhama badlands area. At the upper part of the sequence, there is a Loessial Arid Brown Soil (Calciorthids), characteristic of the mildly arid climate of the area. The soil has two calcic horizons and four clayey layers alternating with four calcareous layers which are beneath them. Physical, chemical, and magnetic‐susceptibility data and micromorphological evidence indicate that each clayey layer together with the calcareous layer beneath it forms a single pedogenic unit. These units are similar to modern Grumusolic soils (Xeric Paleargids or Xererthic Calciargids) that occur in the semiarid belt of the S coastal plain and develop on eolian‐dust parent material. The calcareous layers are in fact calcic horizons formed by leaching of the carbonates from the clayey layers and accumulated in the form of in situ carbonate nodules. The leaching of the carbonates is not complete; they were never completely leached in the past. This feature together with a typical brown color is also characteristic of the modern soils developed in the semiarid water regime of the area. The four superimposed paleosols represent four cycles. It is suggested that they were formed in two phases. During a dry environment, a short phase of rapid eolian‐dust accumulation prevailed, followed by a stable phase of soil development in a somewhat wetter climate. Dating by optically stimulated luminescence and previous dating by ¹⁴C in the area suggest that the upper two paleosol cycles belong to the Last Glacial period whereas the other two cycles are of an earlier age. The magnetic‐susceptibility values decrease with age and react different from temperate areas. Below the four cycles, two totally leached paleosols developed on sandy parent material occur. Both paleosols have a reversed magnetic polarity and are hence older than 780 ky BP. The upper one is a Brown Mediterranean soil, and the lower one is a Red Mediterranean soil. Thin‐section evidence suggests that they formed on terrestrial sand dunes.     

Intensification of redclaw crayfish Cherax quadricarinatus culture: I. Hatchery and nursery system

Intensification of an indoor hatchery and nursery system for the Australian redclaw crayfish Cherax quadricarinatus (von Martens) (Decapoda: Parastacidae) was obtained by increasing the surface area available for the crayfish juveniles and by synchronizing the age of the hatchlings held in each tank. The former improvement was facilitated by distributing an artificial seaweed-like material throughout almost the entire volume of small (275 l) hatching tanks. As the number of egg-bearing females was increased from 3 to 8 per hatching tank, the number of juveniles per liter also increased to as many as 6.5 juveniles/l, without reaching an apparent upper limit. The hatchlings were kept in the tanks for 75 days from the day females were found to be gravid and then harvested and graded according to size. The average juvenile weight at harvest was 0.34±0.04 g. The weight distribution of the juvenile males was not significantly different from that of the juvenile females on the day of harvest, and in both the distribution was positively skewed.     

Application of secondary-treated effluents for cultivation of sunflower (Helianthus annuus L.) and celosia (Celosia argentea L.) as cut flowers

In this study we have examined the effects of irrigation with municipal secondary-treated effluents on growth of sunflower (Helianthus annuus L.) and celosia (Celosia argentea var cristata L.) plants for cut flower production. The applied treated effluents contained higher levels of Na (∼X6), Cl (∼X4.5), N–NH₄ (30–50 mg/l), B, HCO₃, P, K, and Fe than the potable water. The effect of the two types of irrigation water on the chemical composition of soil and leaves, flower yield and postharvest performance was examined. Our results demonstrate higher accumulation of Cl, Na, B, and NO₃ in soils irrigated with effluents as compared to potable water only in the 40–60 cm soil layer. Phosphorus accumulated to higher levels in the upper soil layer (0–20 below ground), as well as in the lower soil layer, irrigated with effluents. SAR levels as well, were higher under irrigation with the treated effluents in the soil until 40 cm below ground indicating higher Na absorption in comparison to Ca and Mg. In both sunflower and celosia, boron accumulated to higher levels in leaves of plants irrigated with effluents. In addition, under irrigation with effluents, celosia accumulated higher levels of N and Mn and sunflower higher levels of P and lower levels of Fe. Despite these differences in mineral contents, the quality of the irrigation water did not affect cut flower yield. In both species spike length, receptacle size, number of flowers and flower weight were not affected. However, flowers’ quality, examined during vase life, was compromised by irrigation with the effluents. Celosia leaves appeared more yellow and sunflower petal edges became browner. Nevertheless, leaf yellowing in celosia occurred mainly on the lower leaves of the stem, which can be removed to maintain high commercial value. The reduction in sunflower quality as well, will most likely not pose any commercial problem since these negative symptoms appeared at a late stage of vase life. Overall, our results demonstrate that secondary-treated municipal effluents can be used for production of sunflower and celosia for cut flowers, in the northern-eastern part of the ‘Negev’ desert in Israel. Due to the high SAR values of the water, and in order to avoid damage to the soil structure, dilution of this water should be considered for commercial growth.