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排序方式: 共有134条查询结果,搜索用时 15 毫秒
91.
It is shown that lignosulphonic acids, at a concentration of 13 % in conventional feed given to growing pigs, lead to a marked reduction in the weight gain.When given 3 % and 6 % lignosulphonic acids in the feed, no effects on the weight gain were observed as compared to the controls.The weight gain reduction caused by lignosulphonic acids at high levels in the feed is discussed in relation to the inhibitory effects of these acids on a number of biological systems which have been studied previously. 相似文献
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Jensen MØ Jogini V Borhani DW Leffler AE Dror RO Shaw DE 《Science (New York, N.Y.)》2012,336(6078):229-233
The mechanism of ion channel voltage gating-how channels open and close in response to voltage changes-has been debated since Hodgkin and Huxley's seminal discovery that the crux of nerve conduction is ion flow across cellular membranes. Using all-atom molecular dynamics simulations, we show how a voltage-gated potassium channel (KV) switches between activated and deactivated states. On deactivation, pore hydrophobic collapse rapidly halts ion flow. Subsequent voltage-sensing domain (VSD) relaxation, including inward, 15-angstrom S4-helix motion, completes the transition. On activation, outward S4 motion tightens the VSD-pore linker, perturbing linker-S6-helix packing. Fluctuations allow water, then potassium ions, to reenter the pore; linker-S6 repacking stabilizes the open pore. We propose a mechanistic model for the sodium/potassium/calcium voltage-gated ion channel superfamily that reconciles apparently conflicting experimental data. 相似文献
94.
Control of the selectivity of the aquaporin water channel family by global orientational tuning 总被引:1,自引:0,他引:1
Tajkhorshid E Nollert P Jensen MØ Miercke LJ O'Connell J Stroud RM Schulten K 《Science (New York, N.Y.)》2002,296(5567):525-530
Aquaporins are transmembrane channels found in cell membranes of all life forms. We examine their apparently paradoxical property, facilitation of efficient permeation of water while excluding protons, which is of critical importance to preserving the electrochemical potential across the cell membrane. We have determined the structure of the Escherichia coli aquaglyceroporin GlpF with bound water, in native (2.7 angstroms) and in W48F/F200T mutant (2.1 angstroms) forms, and carried out 12-nanosecond molecular dynamics simulations that define the spatial and temporal probability distribution and orientation of a single file of seven to nine water molecules inside the channel. Two conserved asparagines force a central water molecule to serve strictly as a hydrogen bond donor to its neighboring water molecules. Assisted by the electrostatic potential generated by two half-membrane spanning loops, this dictates opposite orientations of water molecules in the two halves of the channel, and thus prevents the formation of a "proton wire," while permitting rapid water diffusion. Both simulations and observations revealed a more regular distribution of channel water and an increased water permeability for the W48F/F200T mutant. 相似文献
95.
Parducci L Jørgensen T Tollefsrud MM Elverland E Alm T Fontana SL Bennett KD Haile J Matetovici I Suyama Y Edwards ME Andersen K Rasmussen M Boessenkool S Coissac E Brochmann C Taberlet P Houmark-Nielsen M Larsen NK Orlando L Gilbert MT Kjær KH Alsos IG Willerslev E 《Science (New York, N.Y.)》2012,335(6072):1083-1086
It is commonly believed that trees were absent in Scandinavia during the last glaciation and first recolonized the Scandinavian Peninsula with the retreat of its ice sheet some 9000 years ago. Here, we show the presence of a rare mitochondrial DNA haplotype of spruce that appears unique to Scandinavia and with its highest frequency to the west-an area believed to sustain ice-free refugia during most of the last ice age. We further show the survival of DNA from this haplotype in lake sediments and pollen of Tr?ndelag in central Norway dating back ~10,300 years and chloroplast DNA of pine and spruce in lake sediments adjacent to the ice-free And?ya refugium in northwestern Norway as early as ~22,000 and 17,700 years ago, respectively. Our findings imply that conifer trees survived in ice-free refugia of Scandinavia during the last glaciation, challenging current views on survival and spread of trees as a response to climate changes. 相似文献
96.
Morten Tryland Christian Lydersen Kit M. Kovacs Stein Istre Thoresen 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(4):501-506
Background: Information regarding health and disease is limited for walruses, a keystone species in arctic marine ecosystems. Serum chemistry analysis is a useful clinical tool for the health assessment of walruses, but only a few captive Pacific walruses have been evaluated. Objectives: The aim of this study was to determine serum chemistry reference values for free‐ranging male Atlantic walruses (Odobenus rosmarus rosmarus) on Svalbard and to assess potential differences in animals with low and high tissue levels of organic pollutants. Methods: Blood samples were collected from 17 wild, adult, male Atlantic walruses chemically immobilized with etorphine at eastern Svalbard (Norway). Serum was obtained for routine biochemical analysis as well as nonesterified free fatty acids (NEFA) and cortisol tests. Serum protein concentration was also measured by agarose gel electrophoresis. Results: Reference values (ranges) included alanine aminotransferase (12–51 U/L), aspartate aminotransferase (54–137 U/L), alkaline phosphatase (42–243 U/L), creatine kinase (32–506 U/L), lactate dehydrogenase (480–1322 U/L), amylase (0–23 U/L), lipase (68–298 U/L), total protein (68–91 g/L), albumin (25.3–34.8 g/L), creatinine (84–137 μmol/L), urea (8.2–19.9 mmol/L), bilirubin (0–4 μmol/L), cholesterol (4.4–7.3 mmol/L), NEFA (0.1–0.4 mmol/L), triglycerides (0.6–2.2 mmol/L), calcium (2.0–2.7 mmol/L), phosphorus (1.7–2.8 mmol/L), sodium (147–162 mmol/L), potassium (4.7–7.4 mmol/L), chloride (102–115 mmol/L), and cortisol (<28–214 nmol/L). Walruses exposed to high levels of organic pollutants (n=6) had significantly lower (P=.022) phosphorus concentration than those with low levels of pollutants (n=6). Conclusions: The clinical chemistry reference values determined in this study can serve as baseline data for future health‐related studies of walruses in a changing Arctic and may also be helpful for health evaluations of walruses in captivity. Impacts of the exposure of marine mammals to organic pollutants should be further investigated. 相似文献
97.
Erik Larn y Sebastian Dantz Morten Eikenes Holger Militz 《Wood material science & engineering》2006,1(2):59-68
Chitosan is a biopolymer derived from chitin in crustacean shells. Over the past decade it has been studied as an environmentally benign wood-protecting agent. It is assumed to act as a fungi-stat against a wide range of fungi and even as a fungicide at higher concentrations. This study investigated the properties of wood treated with modified chitosan of different molecular weights. Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) samples were impregnated with two chitosan solutions differing in their average molecular weights. The chitosan solutions were depolymerized by nitrous acid to one solution of high molecular weight and one solution of low molecular weight with a concentration of 5% (w/v). The results show changes in sorption properties, antifungal properties, fire-retardant properties and mechanical properties of modified chitosan-treated wood. Heat-modified, chitosan-treated wood showed similar properties to chitosan-treated wood, except for brownish coloration, enhanced hydrophobation, and slightly reduced antifungal and fire-retardant properties. The modulus of rupture and hardness showed little or no change. The modulus of elasticity of the heat-modified, chitosan-treated wood increased by 27% compared with untreated wood. 相似文献
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Wasana Suebsong Supawadee Poompuang Prapansak Srisapoome Skorn Koonawootrittriron Amorn Luengnaruemitchai Harry Johansen Morten Rye 《Journal of fish diseases》2019,42(11):1553-1562
The potential of selection to improve resistance to streptococcosis was evaluated in a commercial population of Nile tilapia in Thailand. The base generation (G0) consisted of offspring from 98 sires and 149 dams using a partly nested design. At 60 days post‐hatch, 30 fish from each family were injected intraperitoneally with a Streptococcosis agalactiae solution (1 × 109 CFU/ml) and evaluated for 14 days. Disease resistance was recorded as the number of days from challenge until death (DD) and as a binary (BIN) trait (dead/alive) on day 14. Three models were used for genetic analyses: Cox frailty model for DD; animal model for DD; and animal model for BIN. Age at challenge was fitted as a covariate and contemporary group as fixed or random effect, depending on the model. Fish from the 18 most resistant families were selected to produce the first generation (G1). Heritability estimates for G0 were 0.22, 0.14 ± 0.02 and 0.11 ± 0.02 for the Cox, linear DD and linear BIN models, respectively. Selection response indicated that the risk of death decreased to 54%, survival time increased to 3.4 days and survival rate increased to 21%. These results suggest that genetic improvement is possible for this population. 相似文献