Analysis of fiber content in flax stems by near-infrared spectroscopy

The conventional means of measuring the fiber content of flax is time-consuming and laborious, and the results obtained vary with the analysis technique used. The plant tissues must first be "retted", a process by which the fibers are separated from the rest of the stem, either by indigenous organisms in the soil when the stems are left in the field or by water (anerobic bacteria) or enzymatic retting. The fiber content is then determined by mechanical or manual separation. In this study, fiber content of flax stems was measured rapidly and objectively by near-infrared spectroscopy (NIRS) using whole pieces of stem in a large cell, in reflectance mode. Compared to the conventional method, the standard error of performance of the NIRS method was between 0.96 and 1.45% (dry matter basis), depending on the model and data processing used. NIRS calibrations were generated by hand separation of fiber from water-retted specimens. The water retting procedure takes several days to complete and requires considerable trained labor to complete the hand separation step. The NIRS procedure was conducted on pieces of stem to simulate measurement in the field.     

Ion leaching from a sugar maple forest in response to acidic deposition and nitrification

Year-to-year variation in acidic deposition within a mature sugar maple-dominated forest and in leaching of ions from the associated podzolic soil were examined at the Turkey Lakes Watershed between 1981 and 1986. Below-canopy inputs to the soil of SO₄ ^2? and NO₃ ^? in throughfall averaged 640 and 295 eq. ha^?1 yr^?1; the corresponding ranges were 493–917 and 261–443 eq. ha^?1 yr^?1. The contribution of atmospheric deposition to SO₄ ^2? NO₃ ^? and Ca²⁺ leaching decreased over the six years. During the study period, the mean annual volume-weighted NO₃ ^? concentration decreased in throughfall and forest-floor percolate and increased in the mineral-soil solution collected below the effective rooting zone. A substantial shift in the balance between SO₄ ^2? and NO₃ ^?leaching from the mineral soil was observed; leaching of SO₄ ^2?decreased and NO₃ ^? leaching increased with time. Leaching of Ca²⁺ and Mg²⁺ from the soil was increased as a result of excess NO₃ ^? production in the soil. The calculated output of NO₃ ^? from the soil, which averaged 1505 eq. ha^?1 yr^?1, considerably exceeded the atmospheric deposition of NO₃ ^?, whereas SO₄ ^2? outputs were only moderately greater than inputs.     

Determination of Heavy Metal Concentrations and Metal Fingerprints of Sewage Sludge from Eastern Cape Province,South Africa by Inductively Coupled Plasma – Mass Spectrometry (ICP-MS) and Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS)

Sewage sludge from Schornville, Zwelitsha and Dimbaza Sewage Treatment Plants (STPs) in the Eastern Cape province, South Africa were analyzed for the heavy metal contents by inductively coupled plasma-mass spectrometry (ICP) while metal fingerprints of the sludge were determined by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Levels of Cd ranged from 1.1 mg kg^-1 at Zwelitsha STP to1.9 mg kg^-1 at Shornville STP while the levels of Zn varied between 1600 mg kg^-1 also at Zwelitsha STP and 4100 mg kg^-1 at Dimbaza STP. The levelsof Ni also ranged from 37 mg kg^-1 again at Zwelitsha STP to 86 mg kg^-1 at Dimbaza STP while the levels of Cr varied between 70 mg kg^-1 at Zwelitsha STP) and 120 mg kg^-1 at Dimbaza STP). The levels of Cu varied between 245 mg kg^-1 at Zwelitsha STP and 441 mg kg^-1 at Dimbaza STP while the levels of Pb ranged from 69 mg kg^-1 at Zwelitsha STP and 365 mg kg^-1 at Dimbaza STP. The fingerprint studies showed that metals like Si, K, Cu, Pt, Ni and Ag probably havenatural bedrock sources from the study area while others like Zn, Pb and Cd may have anthropogenic sources in addition to natural sources. Generally, sludge from Zwelitsha STP has the lowest concentration of heavy metals and has the greatest potential for agricultural use whilesludge from Dimbaza STP has the highest concentration of heavy metals with the least potential for agricultural use.     

Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8

Mouse L-cells transfected with bovine CD8 and two Theileria parva-infected cloned T cell lines expressing bovine CD8 were used to screen the panel of ten monoclonal antibodies (mAbs) submitted to the workshop. Eight of the ten mAbs reacted with the transfectant and both the cloned T cell lines. However, two mAbs CC58 and BAT82A did not recognise the transfectant and only reacted with one of the T cell lines. Further biochemical studies indicated that the eight mAbs react with both homo- and heterodimeric forms of bovine CD8 whilst the two mAbs CC58 and BAT82A react with only heterodimeric forms. These data suggest that bovine DC8 is encoded by two genes as is the case in mouse and man.     

Maximizing First-Year Growth of Mixed-Sex Blue Tilapia Oreochromis aureus in Polyculture with Catfish Ictalurus spp.

Mixed-sex blue tilapia Oreochromis aureus fry were produced from indoor spawns in March and April. Fry were stocked in May at an average weight of 0.5 g at 0, 250, 750, or 2,500/ha into ponds which had been stocked previously with three catfish strains at a combined density of 10,000 fingerlings/ha. After a 167-d culture period, from 15 May until 4 November, tilapia stocked at 250 or 750/ha grew to average harvest size approaching 0.45 kg. No significant difference in average size was observed between males and females except at the 2,500 tilapia/ha stocking density. Sixty percent of the tilapia polyculture ponds yielded no reproduction at harvest and maximum reproduction observed was 13.5 kg/ha. Lack of reproduction and the undeveloped state of sampled female ovaries indicated that most females had yet to attain sexual maturity. Catfish production and catfish feed conversion was not significantly different between tilapia polyculture and catfish monoculture ponds ( P > 0.05). First seine hauls yielded over 88% of the total catfish harvest, but only an average of 3.9% of the marketable tilapia. Tilapia had no measurable impact on the incidence of catfish off-flavor at any of three sample intervals. Overall average levels of TAN and nitrites did not differ significantly between treatments; however, mean chlorophyll a concentration was significantly higher ( P > 0.05) in the 2,500 tilapia/ha treatment than at lower tilapia densities.     

Induction of immunity against infection with Theileria parva (East Coast fever) in cattle using plasma membranes from parasitized lymphoblasts

Protection against challenge with Theileria parva was conferred on three of four calves given three or four inocula of plasma membranes prepared from 6 to 12 X 10(8) autologous parasitized lymphoblasts from cultured cell lines. In contrast, calves remained susceptible to infection following immunization with membranes prepared from allogeneic parasitized lymphoblasts. Similarly, calves vaccinated with either gamma-irradiated autologous or allogeneic infected cells also died of East Coast fever after challenge. The results raise the possibility of vaccination against T. parva using subcellular material from infected lymphoblasts.     

Spread of pseudorabies virus among breeding swine in quarantined herds.

In theory, pseudorabies virus (PRV) may be eliminated from any size of breeding herd by phased test and removal if replacement gilts are not infected with PRV, culling decisions are partially based on PRV status, and the cull rate is higher than the incidence rate of PRV. Annual cull rates are commonly at least 50%, but little information exists on the incidence of PRV within enzootically infected swine herds. The purpose of this study was to develop a method by which spread of PRV could be detected among breeding swine within enzootically infected herds and to determine the incidence of PRV infection in these herds. Data were collected from 17 herds that were quarantined for PRV and ranged in size from 120 to 1,100 sows. At each herd, within the first 5 days of introduction, a group of approximately 30 replacement gilts was identified, vaccinated with a glycoprotein X-deleted PRV vaccine, and blood sample was collected. The owner of 1 herd had a nonvaccinated breeding herd and elected to leave incoming gilts nonvaccinated. After vaccination, blood samples were collected every 1 to 2 months for an average of 13.6 months. Serum samples from vaccinated gilts were tested for antiglycoprotein X antibodies by a specific differential ELISA. Samples from nonvaccinated gilts were evaluated by serum neutralization test. Product-limit method was used to estimate the probability of not becoming infected with PRV. Spread was detected in 7 of 8 herds that had more than 400 sows and in 2 of 9 herds that had less than 400 sows.(ABSTRACT TRUNCATED AT 250 WORDS)     

Hypersomatotropism and insulin-resistant diabetes mellitus in a cat

In this report, we described the clinical, radiographic, and echocardiographic findings in a cat with hypersomatotropism and insulin-resistant diabetes mellitus. Growth hormone determinations were made because of persistent hyperglycemia despite insulin requirements exceeding 2.2 U/kg of body weight, and the acromegalic features of the cat. Also, the results of a therapeutic trial in which a long-acting analogue of somatostatin was used are discussed.     

The Microanatomy of the Coyote's (Canis latrans) Respiratory System

A Light, scanning and transmission electron microscopic study of the coyote respiratory system was carried out, utilizing ten coyotes of both sexes. The animals ranges in age from 8 to 24 months and were in good health. Comparison of the respiratory tissues of the coyote with other Canid species are made.     

Primary Ciliary Dyskinesia in the Dog

Electron microscopy was used to diagnose primary ciliary dyskinesia in a litter of English pointer dogs and in a golden retriever dog. A technique of membrane solubilization, fixation, and negative staining with glutaraldehyde tannic acid identified abnormally constructed central and B microtubules in respiratory cilia from dogs with primary ciliary dyskinesia. Shortened outer dynein arms commonly associated with primary ciliary dyskinesia actually represents the absence of a specific subset of the three most peripheral components of the whole outer dynein arm structure.