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971.
Ellen Kandeler Arvin R. Mosier Jack A. Morgan Daniel G. Milchunas Jennifer Y. King Sabine Rudolph Dagmar Tscherko 《Soil biology & biochemistry》2008,40(1):162-171
Using open-top chambers (OTC) on the shortgrass steppe in northern Colorado, changes of microbial community composition were followed over the latter 3 years of a 5-year study of elevated atmospheric CO2 as well as during 12 months after CO2 amendment ended. The experiment was composed of nine experimental plots: three chambered plots maintained at ambient CO2 levels of 360±20 μmol mol?1 (ambient treatment), three chambered plots maintained at 720±20 μmol mol?1 CO2 (elevated treatment) and three unchambered plots. The abundance of fungal phospholipid fatty acids (PLFAs) shifted in the shortgrass steppe under the influence of elevation of CO2 over the period of 3 years. Whereas the content of the fungal signature molecule (18:2ω6) was similar in soils of the ambient and elevated treatments in the third year of the experiment, CO2 treatment increased the content of 18:2ω6 by around 60% during the two subsequent years. The shift of microbial community composition towards a more fungal dominated community was likely due to slowly changing substrate quality; plant community forage quality declined under elevated CO2 because of a decline of N in all tested species as well as shift in species composition towards greater abundance of the low forage quality species (Stipa comata). In the year after which CO2 enrichment had ceased, abundances of fungal and bacterial PLFAs in the post-CO2 treatment plots shifted slowly back towards the control plots. Therefore, quantity and quality of available substrates had not changed sufficiently to shift the microbial community permanently to a fungal dominated community. We conclude from PLFA composition of soil microorganisms during the CO2 elevation experiment and during the subsequent year after cessation of CO2 treatment that a shift towards a fungal dominated system under higher CO2 concentrations may slow down C cycling in soils and therefore enhance C sequestration in the shortgrass steppe in future CO2-enriched atmospheres. 相似文献
972.
Morgan RA Dudley ME Wunderlich JR Hughes MS Yang JC Sherry RM Royal RE Topalian SL Kammula US Restifo NP Zheng Z Nahvi A de Vries CR Rogers-Freezer LJ Mavroukakis SA Rosenberg SA 《Science (New York, N.Y.)》2006,314(5796):126-129
Through the adoptive transfer of lymphocytes after host immunodepletion, it is possible to mediate objective cancer regression in human patients with metastatic melanoma. However, the generation of tumor-specific T cells in this mode of immunotherapy is often limiting. Here we report the ability to specifically confer tumor recognition by autologous lymphocytes from peripheral blood by using a retrovirus that encodes a T cell receptor. Adoptive transfer of these transduced cells in 15 patients resulted in durable engraftment at levels exceeding 10% of peripheral blood lymphocytes for at least 2 months after the infusion. We observed high sustained levels of circulating, engineered cells at 1 year after infusion in two patients who both demonstrated objective regression of metastatic melanoma lesions. This study suggests the therapeutic potential of genetically engineered cells for the biologic therapy of cancer. 相似文献
973.
First detection of Edwardsiella ictaluri (Proteobacteria: Enterobacteriaceae) in wild Australian catfish
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E Kelly P A J Martin S Gibson‐Kueh D L Morgan B C Ebner J Donaldson N Buller D A Crook S Brooks A M Davis M P Hammer L Foyle S Hair A J Lymbery 《Journal of fish diseases》2018,41(2):199-208
The bacterium Edwardsiella ictaluri is considered to be one of the most significant pathogens of farmed catfish in the United States of America and has also caused mortalities in farmed and wild fishes in many other parts of the world. E. ictaluri is not believed to be present in wild fish populations in Australia, although it has previously been detected in imported ornamental fishes held in quarantine facilities. In an attempt to confirm freedom from the bacterium in Australian native fishes, we undertook a risk‐based survey of wild catfishes from 15 sites across northern Australia. E. ictaluri was detected by selective culturing, followed by DNA testing, in Wet Tropics tandan (Tandanus tropicanus) from the Tully River, at a prevalence of 0.40 (95% CI 0.21–0.61). The bacterium was not found in fishes sampled from any of the other 14 sites. This is the first report of E. ictaluri in wild fishes in Australia. 相似文献
974.
Ammonium and nitrate transport during saturated and unsaturated water flow through sandy soils
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Davie M. Kadyampakeni Peter Nkedi-Kizza Jorge A. Leiva Augustine Muwamba Evelyn Fletcher Kelly T. Morgan 《植物养料与土壤学杂志》2018,181(2):198-210
Citrus production in Florida accounts for ≈ 60% of national production in USA. The sandy soil characteristic (> 95% sand) makes water and nutrient management extremely difficult, raising concerns about environmental sustainability as a result of nutrient inputs in citrus producing regions where sandy soils dominate. Thus, laboratory column and field experiments were conducted to better understand the leaching patterns of and ions in Florida's sandy soils. The soil columns were first saturated from the bottom with two pore volumes of simulated Florida rain followed by pumping a pulse of fertilizer mixture at a steady Darcy flux of 14 cm h?1. Nitrate and Cl? appeared earlier in the effluent than in the A and Bh horizons, due to cation exchange of . Essentially identical breakthrough curves (BTCs) for and were observed in the E‐horizon, due to very low sorption of . The convective and dispersive equilibrium (CDE) model simulations were in good agreement with measured breakthrough curves (BTCs) for , , and Cl?. However, the sorption coefficient (KD) values used in the CDE model to simulate the BTCs for were about 10 times less than the batch isotherm KD values. This was attributed to differences in pH, cation composition, and ionic strength between batch (static) and dynamic (leaching) systems. The field experiment showed that under unsaturated flow, improved short‐pulse fertigation systems (drip and microsprinkler) limited and transport beyond the root zone (top 30 cm), which might have promoted nutrient and water uptake in citrus. The column study revealed that under extreme weather events such as hurricanes or storm surge in Florida, saturated soil conditions can trigger N mobility below the root zone to surficial or groundwater aquifers. In the field experiment, the use of judicious, minimal and split applications and accurate placement of N‐fertilizers reduced leaching of N especially during heavy storms in the summer rainy months of Florida. The field experiment demonstrated that it is possible to manage inorganic N forms for optimal residence time for uptake and minimal leaching concerns. 相似文献
975.
The aim of this study was to evaluate the effects of dietary soluble non-starch polysaccharide (sNSP) content and xylanase supplementation on production performance, egg quality parameters, and nutrient digestibility in Hy-line Brown layers from 25 to 32 wk of age. A total of 144 Hy-line Brown laying hens (25 wk of age) were randomly allocated to 1 of 4 wheat-based dietary treatments in a 2 × 2 factorial experimental design, with 36 replicates of individual hens per treatment. The diets were formulated to contain either a high or low sNSP level (at 13.3 or 10.8 g/kg) and were supplemented with either 0 or 12,000 BXU/kg exogenous xylanase. Birds were fed these treatment diets for an 8-wk period, and hen production performance, including daily egg production, average egg weight, daily egg mass, feed conversion ratio and proportion of dirty and abnormal eggs were measured at bird age 25 to 28 wk and 29 to 32 wk. An interaction between sNSP content of the diet and xylanase supplementation was observed on daily egg production from 25 to 28 wk of age (P = 0.018); birds fed the high sNSP diet without xylanase had lower egg production than those fed any other treatment. An interaction between the 2 dietary factors was also observed on hen weight gain at 29 to 32 wk of age (P = 0.014), with birds fed the low sNSP diet with 12,000 BXU/kg xylanase presenting greater weight gain compared to those fed the high sNSP diet with 12,000 BXU/kg xylanase. Feed intake at 29 to 32 wk of age was reduced by xylanase supplementation (P = 0.047). Xylanase supplementation also increased yolk colour score at both 28 and 32 wk of age, and decreased yolk weight at 32 wk of age (P = 0.014, 0.037 and 0.013, respectively). Birds fed the low sNSP diet presented lower protein digestibility (P = 0.024) than those fed the high sNSP diet. Additionally, birds fed high sNSP presented higher shell reflectivity at both 28 and 32 wk of age (P = 0.05 and 0.036, respectively). The influence of duration of feeding the treatment diets on egg quality was also determined. It was observed that egg weight, yolk weight and yolk colour score consistently increased over time, regardless of experimental treatment effects. In contrast, Haugh Unit and albumen height significantly decreased throughout the study period in all treatments, although this was less pronounced in hens fed the treatment with high sNSP and no supplemental xylanase. A reduction in shell breaking strength over time was observed only in hens fed the treatments without xylanase addition, and shell thickness was improved over time only in birds fed the low sNSP diet with xylanase. The impacts of the dietary treatments were largely inconsistent in this study, so a solid conclusion cannot be drawn. However, these findings do indicate that dietary NSP level influences layer production performance, and thus should be considered when formulating laying hen diets. It also proved that further research is warranted into how to optimize the benefits of xylanase application in laying hens. 相似文献
976.
Sosthene Musigwa Natalie Morgan Robert A. Swick Pierre Cozannet Sarbast K. Kheravii Shu-Biao Wu 《动物营养(英文)》2021,7(2):496
This study evaluated the effect of multi-carbohydrase (MC) on energy and nitrogen (N) balance and gene expression in broilers fed diets with different crude protein (CP) contents. The study employed a 2 × 2 factorial arrangement of treatments. The factors were presence or absence of MC, and standard (SCP) or low (LCP) dietary CP concentration. A 3-phase feeding program was used, including starter (0 to 7 d), grower (8 to 17 d) and finisher (18 to 28 d) phases. The study was undertaken in closed calorimetry chambers. Each of the 4 dietary treatments was replicated 8 times in total across 2 runs, with 2 birds per replicate (n = 64). Data for energy partitioning and N balance were collected from d 25 to 28. On d 28, birds were euthanized to collect muscle and intestinal tissue samples for gene expression. The results showed that the MC increased apparent metabolizable energy (AME, P < 0.01) and net energy (NE, P < 0.05), and reduced the feed conversion ratio (FCR, P < 0.01) in all diets. The proportion of energy retained as fat per total energy retention (REf/RE) was positively correlated with feed AME and NE (r = 0.541, P < 0.01 and r = 0.665, P < 0.001, respectively), suggesting that feed energy augmented with increased fat gain. Muscle ATP synthase subunit alpha (ATP5A1W) gene expression had a positive correlation with REf/RE and feed NE (r = 0.587, P < 0.001 and r = 0.430, P < 0.05, respectively). Similarly, muscle peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1A) expression was negatively correlated with weight gain and positively correlated with FCR (r = −0.451, P < 0.05 and r = 0.359, P < 0.05, respectively). These correlations show that over-expressions of muscle genes related to energy production reduce bird performance. This study demonstrated that MC increase dietary energy utilization, regardless of dietary CP concentration. However, the energy released by the enzymes increases feed energy-to-CP ratio, meaning there is excess energy that is then deposited as body fat. This suggests that supplemental MC in broiler feeds is beneficial if diets are formulated to contain marginal energy levels. 相似文献