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101.
Tokateloff N Carmalt J Manning S 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(5):519-523
A horse was initially diagnosed with hemarthrosis and desmitis of the long medial collateral ligament of the right tarsus and later developed prominent enthesiophytosis at the site of insertion of the ligament's deep portion. Hemarthrosis due to intra- or peri-articular pathology can cause recurrent lameness, even without evident external trauma. 相似文献
102.
Bourgès-Abella N Geffré A Concordet D Braun JP Trumel C 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(3):303-315
Background: The laser‐based Sysmex XT‐2000iV hematology analyzer is increasingly used in veterinary clinical pathology laboratories, and instrument‐specific reference intervals for dogs are not available. Objective: The purpose of this study was to establish canine hematologic reference intervals according to International Federation of Clinical Chemistry and Clinical and Laboratory Standards Institute guidelines using the Sysmex XT‐2000iV hematology analyzer. Methods: Blood samples from 132 healthy purebred dogs from France, selected to represent the most prevalent canine breeds in France, were analyzed. Blood smears were scored for platelet (PLT) aggregates. Reference intervals were established using the nonparametric method. PLT and RBC counts obtained by impedance and optical methods were compared. Effects of sex and age on reference intervals were determined. Results: The correlation between impedance (I) and optical (O) measurements of RBC and PLT counts was excellent (Pearson r=.99 and .98, respectively); however, there were significant differences between the 2 methods (Student's paired t‐test, P<.0001). Differences between sexes were not significant except for HCT, PLT‐I, and PLT‐O. WBC, lymphocyte, and neutrophil counts decreased significantly with age (ANOVA, P<.05). Median eosinophil counts were higher in Brittany Spaniels (1.87 × 109/L), Rottweilers (1.41 × 109/L), and German Shepherd dogs (1.38 × 109/L) than in the overall population (0.9 × 109/L). PLT aggregates were responsible for lower PLT counts by the impedance, but not the optical, method. Conclusion: Reference intervals for hematologic analytes and indices were determined under controlled preanalytical and analytical conditions for a well‐characterized population of dogs according to international recommendations. 相似文献
103.
Hordeaux J Deniaud J Bemelmans I Bertrand L Moreau S Amiaud J Wyers M Cherel Y Colle MA 《Veterinary pathology》2011,48(3):616-626
Mucopolysaccharidosis (MPS) types I and VII are inborn errors of metabolism caused by mutation of enzymes involved in glycosaminoglycan catabolism, which leads to intralysosomal accumulation of glycosaminoglycans. In children, severe forms of MPS I and VII are characterized by somatic and neurologic manifestations, including a poorly understood hearing loss. The purpose of this study is to describe the age-related histopathologic changes of the ear in spontaneous canine models of MPS I and VII. Pathologic changes in the ear were assessed in MPS I and VII dogs ranging from 1.6 to 9.3 months of age. Paraffin-embedded sections of the whole ear and Epon-embedded semithin sections of the cochlea were examined. The following lesions were blindly scored in the middle and inner ear: inflammation, cells vacuolization, thickening of osseous and membranous structures, perivascular vacuolated macrophages infiltration, and bone resorption. All dogs had lysosomal storage within cells of tympanic membrane, ossicles, tympanic bone and mucosa, cochlear bone, spiral ligament, limbus, and stria vascularis. The MPS I dogs mainly had progressive cochlear lesions. The MPS VII dogs had severe and early middle ear lesions, including chronic otitis media and bone resorption. The MPS I dog only partially recapitulates the pathology seen in humans; specifically, the dog model lacks inflammatory middle ear disease. In contrast, the MPS VII dog has severe inflammatory middle ear disease similar to that reported in the human. In conclusion, the canine MPS VII model appears to be a good model to study MPS VII-related deafness. 相似文献
104.
Vanden Bussche J Kiebooms JA De Clercq N Deceuninck Y Le Bizec B De Brabander HF Vanhaecke L 《Journal of agricultural and food chemistry》2011,59(10):5786-5792
In recent years, questions have been raised on the possible semi-endogenous status of the alleged xenobiotic thyreostatic drug thiouracil; thiouracil has been detected in the urine of various animals (livestock and domesticated) at concentrations between 1 and 10 μg L(-1) and also in human urine. Although several studies suggest Brassicaceae-derived feed as potential origin, no traces of thiouracil have been detected in feed so far. Therefore, the aim of this study was to elucidate the origin of thiouracil in the urine of livestock and humans. To this purpose various Brassicaceae feed and food sources (e.g., rapeseed, rapeseed coarse meal, cabbage, cauliflower, broccoli) were investigated for the presence of thiouracil. In addition, the impact of the Brassicaceae-related β-thioglucosidase enzyme was evaluated. This myrosinase enzyme appeared to be crucial, because without its catalyzed hydrolysis no thiouracil could be detected in the various Brassicaceae-derived samples. Therefore, a sample pretreatment with incorporated enzymatic hydrolysis was developed after ensuring the quality performance of the extracted myrosinase mixture with a single-point glucose assay. Upon enzymatic hydrolysis and LC-MS(2) analysis, thiouracil was successfully detected in samples of traditional rapeseed, rapeseed-'00' variety coarse meal (values of erucic acid <2% and glucosinolates <25 μmol g(-1)), and rapeseed cake at 1.5, 1.6, and 0.4 μg kg(-1), respectively. As for the food samples, broccoli and cauliflower displayed thiouracil concentrations of 6.0 and <1.0 μg kg(-1), respectively. To the best of the authors' knowledge this study is the first to report the presence of naturally occurring thiouracil in feed and food samples. Future research should investigate the pathway of thiouracil formation and identify its possible precursors. 相似文献
105.
106.
B. Dave Oomah Nathalie Tiger Mark Olson Parthiba Balasubramanian 《Plant foods for human nutrition (Dordrecht, Netherlands)》2006,61(2):86-92
Eight lupin (Lupinus angustifolius L.) genotypes grown at four locations in south central Alberta in 2004 were evaluated for variability in phenolic constituents and antioxidant activity measured by a photochemiluminescence assay. Genotype was the main source of variation for content of phenolic compounds and antioxidant activities. Phenolic compounds in genotypes varied minimally from 11.9 to 14.7 mg catechin equivalent and 4.15 to 4.95 mg rutin equivalent g−1 lupin for total phenolic and flavonoid contents, respectively. Lupin genotypes exhibited weak antioxidant activity based on water–soluble substances (ACW) of 0.54 to 1.07 μmole Trolox equivalent antioxidant capacities (TEAC)/g with lag time ranging from 70 to 153 s and an antioxidant index of 6.7 to 14.5 and 1.9 to 3.3 μmole TEAC/g based on measurements of lipid-soluble substances (ACL). Antioxidant activity of lupin genotypes was not related to phenolic contents of seeds. 相似文献
107.
Abed C Legrave N Dufies M Robert G Guérineau V Vacelet J Auberger P Amade P Mehiri M 《Marine drugs》2011,9(7):1210-1219
Chemical investigation of the Mediterranean sponge Sarcotragus spinosulus led to the isolation of a new hydroxylated nonaprenylhydroquinone, along with two known metabolites, hepta- and octaprenylhydroquinones. The structure of the new metabolite was assigned by extensive 1D and 2D NMR analyses and MS studies. The antileukemic effect of the three compounds towards the chronic myelogenous leukemia (CML) cells line K562 was also evaluated. 相似文献
108.
109.
Guylaine Blanc Alain Charcosset Jean-Baptiste Veyrieras André Gallais Laurence Moreau 《Euphytica》2008,161(1-2):71-84
Many studies already investigated marker-assisted selection (MAS) efficiency but mainly in biparental populations. Connected
multiparental populations address a broader diversity and confer a gain of power for QTL detection which must be beneficial
for MAS. Our objective was to compare multiparental connected designs to biparental populations taken separately for MAS and
phenotypic selection. We first detected QTL for flowering time and grain yield in an experimental maize design involving four
parental inbred lines crossed to produce six different biparental populations and confirmed the advantage of multiparental
connected designs over biparental populations for QTL detection. Based on these results we performed stochastic simulations
to evaluate the expected efficiency of four generations of MAS and phenotypic selection. Different parameters were considered:
trait heritability, genetic architecture and whether QTL were assumed to be known or have to be detected. Genetic gains were
higher in the multiparental design than on average over the biparental populations considered separately, especially when
favourable alleles were equally distributed among parental lines. When QTL detection was included in the simulation process,
we found that type I error risk considered for declaring QTL as significant should be adapted to the design. Type I error
risks leading to the best response were higher for the biparental populations than for the multiparental design. Besides addressing
a broader diversity, multiparental designs increase the power of QTL detection, which reinforces their superiority over biparental
designs for MAS. Application of MAS to multiparental designs therefore appears promising to accelerate genetic gain in plant
breeding programs. 相似文献
110.
Anne-Sophie Sergent Nathalie Bréda Léopoldo Sanchez Jean-Charles Bastein Philippe Rozenberg 《Annals of Forest Science》2014,71(6):709-720