Assessing geopedological soil mapping approach by statistical and geostatistical methods: A case study in the Borujen region,Central Iran

Geopedology, using geomorphologic and supervised classification bases, has been proposed as a soil surveying method. It undertakes a systematic approach to map soilscapes. This approach tries to reduce the budget of accomplishing soil surveys as well as increasing the purity of mapping units. But to what extent it has been succeeded is the question. The main objective of this research was to validate the geopedological mapping methodology by statistical and geostatistical methods in the Borujen region, Central Iran. After a primary interpretation of the study area on air photos (1:20 000 scale), 94 pedons were excavated on a 125 m square sampling grid in the sample area for geostatistical studies. To achieve the statistical aims, a geomorphic unit which encompassed the maximum surface of the sample area (and also the study area) was selected and the biggest delineation of this unit with 19 pedons was considered. The credibility of generalizing the results of the geopedological approach for the studied unit was tested by comparison with 15 pedons in a similar unit outside the sample area, named the validation area. Properties of the soils, including percentage of clay, rock fragments (2–75 mm) and total carbonates in the soil family control section, percentage of organic matter in the A horizon and its thickness, were selected for statistical and geostatistical analyses. Univariate and multivariate statistical analyses obtained from the units in the sample and the validation areas, showed that the means of soil variables were similar. A high difference between A horizon thickness variances in the sample area and the validation area affected the significance of the variation test in the univariate and multivariate results. Our study demonstrated that, when comparing the same factors from sample and validation areas, the spatial distribution and spatial dependency level of soil attributes were different. Therefore, although the geopedological approach tries to distinguish more homogeneous soil mapping units, it is still not able to fully define and represent the real variability of soil properties and show the chaotic behavior or nature of the soils. We recommend further investigations on different techniques of stratifying the landscape in order to better analyze and understand the soil-forming processes and the nature of soil variability and to improve sampling and mapping approaches. We suggest that the additional determination of the phases of landforms and phases of soil series will improve mapping in the future.     

Growth performance,blood biochemical parameters,immune response,and antioxidant defense of Asian seabass (Lates calcarifer) fingerlings exposed to monovalent and bivalent vaccines against Streptococcus iniae and Vibrio harveyi

The present study investigated the effects of monovalent and bivalent vaccines against Streptococcus iniae and Vibrio harveyi on growth performance, blood biochemical parameters, immune system, and antioxidant defense of Asian seabass (Lates calcarifer) fingerlings. Fish were exposed to seven experimental groups containing phosphate buffer saline (control), S. iniae vaccine by injection, S. iniae vaccine by immersion method, V. harveyi vaccine by injection, V. harveyi vaccine by immersion method, S. iniae/V. harveyi vaccines by immersion method, and S. iniae/V. harveyi vaccines by injection in triplicate for 60 days. Vaccination resulted in a significant alteration of the final weight (FW), feed conversion ratio (FCR), daily weight gain (DWG), relative growth rate (RGR), glucose, lysozyme, and complement levels at 60 days post-vaccination. Also, serum glutathione activity significantly increased in group SVIM (S. iniae/V. harveyi vaccines by immersion method) compared to the control group at 60 days post-vaccination. The level of MDA in serum was significantly decreased in SIN (S. iniae vaccine by injection) and SVIM groups compared to the control group 60 days post-vaccination where the value of this parameter was significantly higher in VIM (V. harveyi vaccine by immersion method) and SVIN (S. iniae/V. harveyi vaccines by injection) groups. The results of this study suggest that exposure to monovalent and bivalent vaccines against S. iniae and V. harveyi could alter the growth performance, nonspecific immune response, and antioxidant defense of Asian seabass fingerlings.

     

QTL analysis of resistance to Fusarium head blight in wheat using a 'Wangshuibai'-derived population

Fusarium head blight (FHB) is a devastating disease that reduces the yield, quality and economic value of wheat. For quantitative trait loci (QTL) analysis of resistance to FHB, F₃ plants and F_3:5 lines, derived from a ‘Wangshuibai’ (resistant)/‘Seri82’(susceptible) cross, were spray inoculated during 2001 and 2002, respectively. Artificial inoculation was carried out under field conditions. Of 420 markers, 258 amplified fragment length polymorphism and 39 simple sequence repeat (SSR) markers were mapped and yielded 44 linkage groups covering a total genetic distance of 2554 cM. QTL analysis was based on the constructed linkage map and area under the disease progress curve. The analyses revealed a QTL in the map interval Xgwm533‐Xs18/m12 on chromosome 3BS accounting for up to 17% of the phenotypic variation. In addition, a QTL was detected in the map interval Xgwm539‐Xs15/m24 on chromosome 2DL explaining up to 11% of the phenotypic variation. The QTL alleles originated from ‘Wangshuibai’ and were tagged with SSR markers. Using these SSR markers would facilitate marker‐assisted selection to improve FHB resistance in wheat.     

Effect of nitrogen and potassium fertilizer on growth,yield and chemical composition of sweet fennel

Abstract

In this study, the effect of different levels of nitrogen (N) and potassium (K) fertilizers on vegetative fresh bulb yield, quality and biochemical attributes of sweet fennel were investigated in spring 2017 and 2018. Treatments consisted of 0, 50, 100, or 150?kg ha^?1 of N and K in a randomized complete block design with three replications. Results showed that, the bulb fresh yield increased with application of N and K in both years, while the higher level of K had significant adverse effect in first year. Results also showed that sweet fennel bulb treated by N and K exhibited higher vitamin C, total phenols and flavonoid contents, and antioxidant capacity, especially in first year. In both years, alone or combined application of N and K increased total soluble solids (TSS) content as compared to control, and this effect was more pronounced in 2017 season. Macronutrient contents of bulb including magnesium (Mg), phosphorus (P), K, N, and calcium (Ca) were significantly enhanced by NK fertilizer, however an alone application of K decreased Ca and Mg nutrient contents. Therefore, to improve fresh bulb yield, quality and antioxidant capacity in sweet fennel, 150?kg ha^?1 K and 100?kg ha^?1 N is recommended.     

A new laser scanning system for measuring action potential propagation in the heart

A rapid laser scanning system was developed to map the spread of excitation in amphibian and mammalian hearts stained with fluorescent dye. Isochronic maps of conduction were constructed by timing the upstroke of the optical action potential; 128 sites could be scanned in 4 milliseconds. The accuracy of this technique was verified by recording simultaneously from 16 unipolar electrodes placed in different areas of the heart. Conducted action potentials in normal frog heart propagated at 0.1 meter per second. Propagation of action potentials was also monitored in ischemic cat heart, in which both driven and arrhythmic action potential upstrokes could be tracked. The results suggest that this system is capable of scanning the normal and abnormal spread of electrical activity in the heart.     

Foliar Application of Silicon,Sulfur, and Flowering Fruit Set Biostimulant on Canola

This experiment was conducted to study the effects of foliar application of silicon, sulfur, and flowering fruit set biostimulant on canola in the farmer’s condition in Darzian, 12?km from Marivan city in the northwest of Iran. The experimental layout was designed as a split-split plot in a randomized complete-block design, with three replications during two consecutive growing seasons, 2017–18 and 2018–19. The main factor included silicon application at two levels: control (0: non-application) and silicon application at 2?kg ha^?1. Sub-factor was sulfur spraying at three levels (0, 1, and 2?L ha^?1) and sub-sub-factor was Tecamin flower (Agri Tecno Fertilizantes, Valencia, Spain) spraying as a flowering fruit set biostimulant at three levels (0, 1, and 2?L ha^?1). The results of the combined analysis showed that the number of grains per pod and biomass were increased significantly at the 1% level by silicon application. Sulfur application improved 1000-grain weight, grain yield, and biomass. This increase was 7.42% for grain yield. Tecamin also significantly increased all traits, including fertile and infertile pod numbers, grain number per pod, 1000-grain weight, grain yield, and biomass. As the most important economic traits, Tecamin increased grain yield by 14.12% compared to controls. Among the treatments, the effect of Tecamin on increasing grain yield was superior. In this experiment, some traits were significantly affected by interaction effects of treatments.

     

Identification of microsatellite markers linked with yield components under drought stress at terminal growth stages in durum wheat

Grain yield and yield components are the main important traits involved in durum wheat (Triticum turgidum L.) improvement programs. The purpose of this research was to identify quantitative trait loci (QTL) associated with yield components such as 1000 grain weight (TGW), grain weight per spike (GWS), number of grains per spike (GNS), spike number per m² (SN), spike weight (SW), spike harvest index (SHI) and harvest index (HI) using microsatellite markers. Populations of F₃ and F₄ lines derived from 151 F₂ individuals developed from a cross between Oste-Gata, a drought tolerant, and Massara-1, a drought susceptible durum wheat genotypes, were used. The populations were evaluated under four environmental conditions including two irrigation regimes of drought stress at terminal growth stages and normal field conditions in two growing seasons. Two hundred microsatellite markers reported for A and B genomes of bread wheat were used for parental polymorphism analysis and 30 polymorphic markers were applied to genotype 151 F_2:3 families. QTL analysis was performed using genome-wide single marker regression analysis (SMA) and composite interval mapping (CIM). The results of SMA revealed that about 20% of the phenotypic variation of harvest index and TGW could be explained by Xcfd22-7B and Xcfa2114-6A markers in different environmental conditions. Similarly, Xgwm181-3B, Xwmc405-7B and Xgwm148-3B and marker Xwmc166-7B were found to be associated with SHI and GWS, respectively. A total of 20 minor and major QTL were detected; five for TGW, two for GWS, two for GNS, three for SN, five for HI, two for SHI and one for SW. The mapped QTL associated with ten markers. Moreover, some of these QTL were prominent and stable under drought stress and non drought stress environments and explained up to 49.5% of the phenotypic variation.     

Genotype x environment interaction for durum wheat grain yield and selection for drought tolerance in irrigated and droughted environments in Iran

Durum wheat is grown in the Mediterranean region under stressful and variable environmental conditions. In a 4-year-long experiment, 14 genotypes [including 11 durum breeding lines, two durum (Zardak) and bread (Sardari) wheat landraces, and one durum (Saji) newly released variety] were evaluated under rainfed and irrigated conditions in Iran. Several selection indices [i.e. stress tolerance index (STI), drought tolerance efficiency (DTE), and irrigation efficiency (IE)] were used to characterize genotypic differences in response to drought. The GGE biplot methodology was applied to analyze a three-way genotype-environment-trait data. Combined ANOVA showed that the year effect was a predominant source of variation. The genotypes differed significantly (P < 0.01) in grain yield in the both rainfed and irrigated conditions. Graphic analysis of the relationship among the selection indices indicated that they are not correlated in ranking of genotypes. The two wheat landraces and the durum-improved variety with high DTE had minimum yield reduction under drought-stressed environments. According to STI, which combines yield potential and drought tolerance, the “Saji” cultivar followed by some breeding lines (G11, G8, and G4) performed better than the two landraces and were found to be stable and high-yielding genotypes in drought-prone rainfed environments. The breeding lines G8, G6, G4, and G9 were the efficient genotypes responding to irrigation utilization. In conclusion, the identification of the durum genotypes (G12, G11, and G4) with high yield and stability performance under unpredictable environments and high tolerance to drought stress conditions can help breeding programs and eventually contribute to increasing and sustainability of durum production in the unpredictable conditions of Iran.     

Trend analysis of reference evapotranspiration in the western half of Iran

Reference evapotranspiration (ET_o) is an important element of the hydrological cycle, and changes in ET_o are of great significance for agricultural water use planning, irrigation system design and management. In this study, annual, seasonal and monthly trends in the Penman-Monteith ET_o at 20 meteorological stations during 1966-2005 in the western half of Iran were examined using the Mann-Kendall test, the Sen's slope estimator and the linear regression. Annual analysis of the ET_o series indicated a positive trend in 70% of the stations according to the Mann-Kendall test and the Sen's slope estimator and in 75% of the stations according to the linear regression. The magnitude of significant positive trends in annual ET_o varied from (+)11.28 to (+)2.30 mm/year. On the seasonal scale, stronger increasing trends were identified in ET_o data in winter and summer compared with those in autumn and spring. Meanwhile, the highest numbers of stations with significant trends were found in the monthly ET_o series in February, while the lowest numbers of stations with significant trends were observed in November. Analysis of the impact of climatic variables on the significant increasing trend in ET_o showed that the increasing trend was mainly caused by a significant increase in air temperature during the study period.     

Inactivation and block of calcium channels by photo-released Ca2+ in dorsal root ganglion neurons

Calcium channels are inactivated by voltage and intracellular calcium. To study the kinetics and the mechanism of calcium-induced inactivation of calcium channels, a "caged" calcium compound, dimethoxy-nitrophen was used to photo-release about 50 microM calcium ion within 0.2 millisecond in dorsal root ganglion neurons. When divalent cations were the charge carriers, intracellular photo-release of calcium inactivated the calcium channel with an invariant rate [time constant (tau) approximately equal to 7 milliseconds]. When the monovalent cation sodium was the charge carrier, photorelease of calcium inside or outside of the cell blocked the channel rapidly (tau approximately equal to 0.4 millisecond), but the block was greater from the external side. Thus the kinetics of calcium-induced calcium channel inactivation depends on the valency of the permeant cation. The data imply that calcium channels exist in either of two conformational states, the calcium- and sodium-permeant forms, or, alternatively, calcium-induced inactivation occurs at a site closely associated with the internal permeating site.