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31.
A physical map of the 1-gigabase bread wheat chromosome 3B 总被引:2,自引:0,他引:2
Paux E Sourdille P Salse J Saintenac C Choulet F Leroy P Korol A Michalak M Kianian S Spielmeyer W Lagudah E Somers D Kilian A Alaux M Vautrin S Bergès H Eversole K Appels R Safar J Simkova H Dolezel J Bernard M Feuillet C 《Science (New York, N.Y.)》2008,322(5898):101-104
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach. 相似文献
32.
Cruz-Migoni A Hautbergue GM Artymiuk PJ Baker PJ Bokori-Brown M Chang CT Dickman MJ Essex-Lopresti A Harding SV Mahadi NM Marshall LE Mobbs GW Mohamed R Nathan S Ngugi SA Ong C Ooi WF Partridge LJ Phillips HL Raih MF Ruzheinikov S Sarkar-Tyson M Sedelnikova SE Smither SJ Tan P Titball RW Wilson SA Rice DW 《Science (New York, N.Y.)》2011,334(6057):821-824
The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1. 相似文献
33.
A GIS-based method for the reconstruction of the late eighteenth century forest vegetation in the Prignitz region (NE Germany) 总被引:1,自引:0,他引:1
Our goal was to reconstruct the late eighteenth century forest vegetation of the Prignitz region (NE Germany) at a scale of
1:50,000. We also wanted to relate the historical forest vegetation to the actual and potential natural vegetation. For these
purposes, we selected 15 woody species and transferred relevant data found in historical records from various sources together
with the recent localities of (very) old individuals belonging to these woody species into ArcView GIS. Following multi-step
data processing including the generation of a point density layer using a moving window with kernel estimation and derivation
of vegetation units applying Boolean algebra rules together with information on site conditions, we derived 17 forest communities
corresponding to the potential natural vegetation. We were able to reconstruct the historical forest vegetation for 90% of
the forest area ca. 1780. Only two of the 17 forest communities covered large parts of the forested area. The oak forest with
Agrostis capillaris covered about 44% of the total forest area, and alder forests on fenland made up about 37%. Oak-hornbeam forests with Stellaria holostea comprised slightly less than 6% of the forest area, while all other forest communities comprised less than 1%. The historical
forest vegetation is more similar to the potential forest vegetation and quite different from the actual forest vegetation
because coniferous tree species currently cover approximately two-thirds of the actual forest area. The most beneficial result
of this study is the map of high-resolution historical vegetation units that may serve as the basis for various further studies,
e.g., modelling long-term changes in biodiversity at the landscape scale. 相似文献
34.
Fallovo C Schreiner M Schwarz D Colla G Krumbein A 《Journal of agricultural and food chemistry》2011,59(8):4198-4207
The effect of three different nitrogen (N) supply forms differing in their ammonium-to-nitrate (NH(4):NO(3)) ratio (100% NH(4), 50% NH(4) + 50% NO(3), 100% NO(3)) under three different levels of daily photosynthetic active radiation (PAR) (low, 5.0; medium, 6.8; high, 9.0 mol m(-2) day(-1)) on a range of desirable health-promoting phytochemicals in Brassica rapa subsp. nipposinica var. chinoleifera and Brassica juncea was determined. The 100% NH(4) supply under medium PAR levels led to the highest concentration of glucosinolates based on a low nitrogen/sulfur ratio as well as high levels of carotenoids in the leaves of both Brassica species. However, the 100% NH(4) supply under low and medium PAR levels resulted in low concentrations of flavonoids based on high N concentration in the leaves. Thus, the data provided here have strong implications for crop management strategies aimed at optimizing both the concentration and composition of a range of phytochemicals. 相似文献
35.
Monika Go?mann Alexandra Scholz Frank Hennig Susanne von Bargen Carmen B��ttner 《Gesunde Pflanzen》2011,63(4):175-182
Fusarium oxysporum and Fusarium proliferatum are important causal agents of crown and root rot of asparagus. In order to detect differences in pathogenicity and aggressiveness, two F. proliferatum and five F. oxysporum single spore isolates from asparagus spears from plantings in Austria and Germany, 55 pure cultures of F. oxysporum from asparagus roots from a planting in Hesse, Germany, and a single F. oxysporum isolate from an asparagus shoot collected in Austria were evaluated in a 28-day quick test on Hoagland??s agar in glass culture tubes. Plantlets were inoculated with spore suspensions from each respective isolate after 14 days of growth under sterile, controlled conditions in a growth chamber. A severity scale was used to assess symptoms on roots two weeks after inoculation. The effects of the single-spore isolates on root and shoot fresh weights of the plantlets were also determined. The pathogenicity of the majority of the F. proliferatum and F. oxysporum isolates included in this study was confirmed. Inoculation with pure and single-spore cultures resulted in elevated disease severity in comparison to non-inoculated controls. In particular, the two F. proliferatum isolates were found to be more aggressive than the F. oxysporum isolates. Moreover, all single spore isolates caused a reduction in fresh weight of roots and shoots in comparison to the controls. With respect to differences among asparagus cultivars, ??Ramos??, was found to be more susceptible than ??Ravel??. Overall, the quick test method was found to be capable of evaluating the pathogenicity and aggressiveness of the tested F. oxysporum and F. proliferatum isolates towards asparagus within 28 days. 相似文献
36.
Kristina Bachteler Monika Riedel Nikolaus Merkt Wolfgang Schies Jutta Fröhlin Jens Wünsche 《Journal of plant nutrition》2015,38(6):839-853
Berry shrivel and bunch stem necrosis are the economically most important physiological disorders of grapevine. The nutritional status of the plant is considered as one of the main elicitors for the occurrence of these disorders. For foliar fertilization trials, two vineyards that had previously been affected by both diseases were selected in Southwest Germany, one with the variety ‘Zweigelt’ and one with Pinot Blanc. By the use of potassium, magnesium, and calcium foliar fertilizers, a rapid increase of different nutrients in the plants should be caused and the effects on the diseases observed. In 2010, no significant differences in the fertilization treatments were found for both varieties. In ‘Pinot Blanc’, significantly more berries were affected by berry shrivel in 2011 in plots treated with calcium fertilizer compared to those treated with potassium and magnesium. Plots with magnesium fertilizer showed significantly less berries affected by berry shrivel than the untreated control. 相似文献
37.
Monika Vilkienė Dalia Ambrazaitienė Danutė Karčauskienė Zenonas Dabkevičius 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(8):641-646
ABSTRACTMineralization is the main organic matter conversion process, which leads not only to preservation of organic matter in the soil but also to its sequestration. Soil organic matter has equal value as mineral part if we want to improve soil quality or increase the yield. Because of intensive farming, irresponsible use of mineral fertilizers and natural factors, soil organic matter is decreasing. To counteract this process, different soil-friendly management practices and techniques, such as shallow tillage, no-tillage or direct drilling and application of additional organic matter are used. The objective of the present study was to assess the changes in the intensity of soil organic matter mineralization as influenced by primary soil tillage of different intensity in combination with organic matter incorporation. Long-term studies showed that land management practices differentiated the soil into two layers: upper (0–10?cm) layer containing more moisture and nutrients and lower (10–20?cm) layer comprising less moisture and nutrients. The conditions of aeration in the arable soil layer did not change under the effect of ploughing. In this soil, the rate of mineralization was lower than that in the ploughless tillage treatment. The most active mineralization of soil organic matter in the ploughless tillage treatment occurred in the autumn period, when high level of rainfall promoted the loss of nutrients from the topsoil layer. 相似文献
38.
Dissolved concentrations of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in the influents and effluents of two municipal sewage treatment plants (STPs) were monitored over 16- (Umeå, Sweden) and 22- (?iauliai, Lithuania) day sampling periods. Sampling was performed using a passive sampling technique (semipermeable membrane devices; SPMDs) for sequestration of the dissolved (readily bioavailable) fraction of persistent organic pollutants (POPs). Removal efficiencies for individual low molecular weight (LMW) PAH compounds varied from 84% to levels at which the compounds were not detected in effluents from Umeå. The corresponding efficiencies of the ?iauliai plant were 33–95%. Measurements revealed that dissolved concentrations of most of the PCBs and some of the high molecular weight (HMW) PAHs increased during the conventional wastewater treatment at both plants. The release of dissolved PAHs and PCBs in the effluents from municipal wastewater treatment plants might increase the environmental contamination by readily bioavailable pollutants in the recipient waters; the rivers Umeälven and Kulpè. SPMDs were found to be suitable sampling tools for long-term (weeks-month) integrative monitoring of trace concentrations of the dissolved fraction of hydrophobic pollutants in the wastewater process, since the sampling and clean-up steps were easy to perform. 相似文献
39.
Granvogl M Christlbauer M Schieberle P 《Journal of agricultural and food chemistry》2004,52(10):2797-2802
A stable isotope dilution assay was developed for the quantitation of the potent onion odorant 3-mercapto-2-methylpentan-1-ol (1) using mass chromatography and synthesized [(2)H(2)]-3-mercapto-2-methylpentan-1-ol as the internal standard. Application of the newly developed method on onions from different origins revealed amounts between 8 and 32 microg/kg in raw onions, whereas 34-246 microg was found in sliced, stored (50 min), and then cooked onions. In extracts prepared by simultaneous steam distillation-extraction the highest concentrations of 1 were formed, amounting to >1200 microg/kg. The much higher content of 3-mercapto-2-methylpentan-1-ol in cooked onions suggested its formation from specific, yet unkown, precursors enzymatically formed during cutting of raw onions. 1 was for the first time identified and also quantified in other Allium species such as chives, scallions, and leek, whereas surprisingly garlic and bear's garlic did not contain the aroma compound. 相似文献
40.