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421.
Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions 总被引:1,自引:0,他引:1
Denis Verwilghen Valeria Busoni Monika Gangl Thierry Franck Jean-Philippe Lejeune Laurent Vanderheyden Johann Detilleux Sigrid Grulke Michelle Deberg Yves Henrotin Didier Serteyn 《Research in veterinary science》2009,87(2):319-328
Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers of cartilage degradation and synovial inflammation. A specific radiological scoring and classification system was therefore developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochondrosis, degenerative joint disease and distal interphalangeal joint effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO2 were used as blood markers. The combination of the blood parameters did not seem to correlate with the used scoring system. Coll2-1NO2 levels however tended to increase with poorer radiological class and this could therefore potentially be a useful predictor of the osteoarticular status in the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with distal interphalangeal joint effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels. 相似文献
422.
Monika Martiniaková Radoslav Omelka Birgit Grosskopf Hana Chovancová Peter Massányi Peter Chrenek 《Acta veterinaria Scandinavica》2009,51(1):52
Background
Nickel (Ni) and zinc (Zn) are trace elements present at low concentrations in agroecosystems. Nickel, however, may have toxic effects on living organisms and is often considered as a contaminant. This study reports the effect of peroral administrated Ni or a combination of Ni and Zn on femoral bone structure in rabbits.Methods
One month-old female rabbits were divided into three groups of five animals each. Group 1 rabbits were fed a granular feed mixture with addition of 35 g NiCl2 per 100 kg of mixture for 90 days. In group 2, animals were fed a mixture containing 35 g NiCl2 and 30 g ZnCl2 per 100 kg of mixture. Group 3 without administration of additional Ni or Zn served as control. After the 90-day experimental period, femoral length, femoral weight and histological structure of the femur were analyzed and compared.Results
The results did not indicate a statistically significant difference in either femoral length or weight between the two experimental groups and the control group. Also, differences in qualitative histological characteristics of the femora among rabbits from the three groups were absent, except for a fewer number of secondary osteons found in the animals of groups 1 and 2. However, values for vascular canal parameters of primary osteons were significantly lower in group 1 than in the control one. Peroral administration of a combination of Ni and Zn (group 2) led to a significant decreased size of the secondary osteons.Conclusions
The study indicates that dietary supplementation of Ni (35 g NiCl2 per 100 kg of feed mixture) and Ni-Zn combination (35 g NiCl2 and 30 g ZnCl2 per 100 kg of the mixture) affects the microstructure of compact bone tissue in young rabbits. 相似文献423.
424.
Ekaterina Samoilova Václav Tejnecký Jan Kopecky Ondřej Drábek Adam Stovicek Petra Vokurková Daria Rapoport Karel Němeček Andrea Buresova-Faitova Rostislav Chotěborský Tereza Patrmanová Monika Hromasová Marketa Sagova-Mareckova 《European Journal of Soil Science》2023,74(3):e13369
Microbial communities were studied in redoximorphic microsites of highly heterogeneous Gleysol at a mm scale using 16S and 18S amplicon sequencing to demonstrate if the composition of soil microbes reflects the differences in ferric and ferrous micro-sites. In both explored gley horizons with redoximorphic features (Bg2 and Cg), ferric mottles were significantly enriched with total P and Fe and depleted of O, Si, Al, K and Ca compared with the adjacent ferrous groundmass (SEM–EDS). Ferric mottles were determined as Fe oxide coatings and hypocoatings. In Bg2, both prokaryotic and micro-eukaryotic communities differed significantly between mottles and groundmass in composition of operational taxonomic units (OTUs) and in proportions of phyla, reflecting heterogeneities in the soil properties there. Mottles in Bg2 were characterized by increased proportion of Proteobacteria, decreased proportion of Acidobacteriota among prokaryotes and by dominance of a single proteobacterial OTU from Anaplasmataceae compared to all other samples. The composition of micro-eukaryotes showed an opposite trend, as micro-eukaryotes of Bg2 groundmass were unique among the other horizons, while micro-eukaryotes of Bg2 mottles had similar composition to neighbouring horizons. Microbial communities of adjacent samples were not more similar to each other than communities of randomly selected ones in Bg2 horizon. That suggests that at mm scale, the sample distance does not represent the driving factor of microbial community composition and that the adjacent samples differ rather due to physicochemical factors. The spatial organization of microbial communities revealed in Bg2 has not reappeared in similarly organized Cg horizon, probably due to other overriding factors. The differences revealed between Bg2 and Cg horizons, including granulometric composition, content of crystalline Fe, exchangeable Al, and organic carbon, as well as exposition to groundwater, were discussed as possible reasons of the distinct organization in Cg. The similarity of pro−/eukaryotic communities of adjacent and non-adjacent couples suggests no distance decay pattern at a mm scale. The agreement between patchiness in soil properties and microbial communities was revealed for the first time and confirms the importance of microscale patterns in soil. 相似文献
425.
426.
Monika Frielinghaus Heinz Petelkau Heike Schäfer Klaus Seidel Lothar Müller 《Archives of Agronomy and Soil Science》2013,59(1-2):19-35
The efficiency and acceptance for erosion and compaction control management is not high and therefore not a guarantee for sustainable land use and soil functionality. The best method for increasing acceptance is a regional soil indicator system combined with an environmental indicator system (McRae et al. 2000). Like the concept of “critical load inputs”; for chemical pollutants, this system would make it possible to quantify the soil state and soil condition for decisions concerning the soil carrying or load capacity. The next step is the assessment of the land use pressure on soil in terms of the soil load capacity and the driving forces for land use. These results may determine the response level required: In a balanced situation, Best Management Practices may help ensure sustainability is maintained, slightly disproportional results suggest additional special agricultural management techniques may be needed, while significant differences may indicate the need for additional land use adjustments or changes in technical management. The indicator system is ideal for application in north‐eastern Germany for all moraine areas and the areas at risk to water and wind erosion and soil compaction. 相似文献
427.
Christie JM Arvai AS Baxter KJ Heilmann M Pratt AJ O'Hara A Kelly SM Hothorn M Smith BO Hitomi K Jenkins GI Getzoff ED 《Science (New York, N.Y.)》2012,335(6075):1492-1496
The recently identified plant photoreceptor UVR8 (UV RESISTANCE LOCUS 8) triggers regulatory changes in gene expression in response to ultraviolet-B (UV-B) light through an unknown mechanism. Here, crystallographic and solution structures of the UVR8 homodimer, together with mutagenesis and far-UV circular dichroism spectroscopy, reveal its mechanisms for UV-B perception and signal transduction. β-propeller subunits form a remarkable, tryptophan-dominated, dimer interface stitched together by a complex salt-bridge network. Salt-bridging arginines flank the excitonically coupled cross-dimer tryptophan "pyramid" responsible for UV-B sensing. Photoreception reversibly disrupts salt bridges, triggering dimer dissociation and signal initiation. Mutation of a single tryptophan to phenylalanine retunes the photoreceptor to detect UV-C wavelengths. Our analyses establish how UVR8 functions as a photoreceptor without a prosthetic chromophore to promote plant development and survival in sunlight. 相似文献
428.
Yager JA Gross TL Shearer D Rothstein E Power H Sinke JD Kraus H Gram D Cowper E Foster A Welle M 《Veterinary dermatology》2012,23(2):136-44, e30
A rare congenital dermatosis, characterized by progressive hypotrichosis with variable scaling and crusting, occurred in 10 short-haired kittens in North America and Europe. Lesions appeared at between 4 and 12 weeks of age, commencing on the head and becoming generalized. The tail was spared in two kittens. Generalized scaling was mild to moderate, often with prominent follicular casts. Periocular, perioral, pinnal and ear canal crusting was occasionally severe. The skin was thick and wrinkled in two kittens. Histologically, the main lesion was abnormal sebaceous gland morphology. Instead of regular differentiation from basal cells to mature sebocytes, the glands were composed of a haphazard collection of undifferentiated basaloid cells, some partly vacuolated and a few containing eosinophilic globules. Mitotic figures and apoptotic cells were present in an irregularly thickened follicular isthmus. Lymphocytic mural folliculitis and mild sebaceous adenitis were rare. Orthokeratotic hyperkeratosis and follicular casts were present. Hair follicles were of normal density and were mostly in anagen, but some contained malacic hair shafts. Perforating folliculitis, leading to dermal trichogranuloma formation, occurred occasionally. Further biopsy samples taken at 2 years and at 3 and 4 years, respectively, from two kittens revealed similar but often more severe sebaceous gland lesions. Hair follicles were smaller, with many in telogen. The young age of onset suggests a genetic defect interfering with sebaceous and, possibly, follicular development. These lesions are discussed with reference to studies of mouse mutants in which genetic defects in sebaceous differentiation cause a similar phenotype of hyperkeratosis and progressive alopecia. 相似文献
429.
430.
The present study was designed to see the effects of Angiotensin-II (Ang-II) on buffalo sperm capacitation, acrosome reaction (AR), and its relation to nitric oxide (NO()) production. The extent of capacitation or AR was determined by dual staining while the NO() production was determined by spectrophotometry. The results thus obtained revealed that Ang-II induced capacitation in a concentration and time dependent manner and 200 nM Ang-II was found to be optimal for capacitation as it was comparable to heparin treatment (50.7±2.45% vs. 51.66±2.33%). In capacitated cells the extent of AR induced by Ang-II was significantly higher than the untreated control (48.13±2.31% vs. 22.16±2.11%) and comparable to lysophosphatidyl Choline (LPC) treatment (51.56±1.94%). The NO() production during Ang-II induced capacitation and AR was gradual and time dependent. These levels were significantly higher when compared to control (3.65±0.53 nmoles/10(8)cells vs. 9.12±0.30 nmoles/10(8)cells). All the actions of Ang-II were inhibited in the presence of Losartan but not PD123319, indicating the role of AT1 receptors in these actions. Further the NO() production was also significantly inhibited in the presence of neomycin and trifluoperazine pointing towards the role of phosphoinositide pathway in this process. In conclusion, Ang-II has a concentration and time dependent effect on buffalo sperm capacitation and AR, mediated via the AT1 receptors. Its effect on NO() production may be indirect involving the phosphoinositide pathway. 相似文献