Mapping QTL controlling soybean seed sucrose and oligosaccharides in a single family of soybean nested association mapping (SoyNAM) population

The meal value of Soybean for monogastric animals is determined partly by sucrose, raffinose and stachyose. Of these, sucrose is desirable, while raffinose and stachyose are indigestible, causing flatulence and abdominal discomfort. The objective of this study was to identify quantitative trait loci (QTL) controlling seed sucrose, raffinose, and stachyose in a set of 140 SoyNAM (Nested Association Mapping) recombinant inbred lines (RILs), developed from the cross between lines IA3023 and LD02‐4485. A total of 3,038 SNP markers from the Illumina SoyNAM BeadChip SNP were used to map the QTLs for sucrose and the RFOs, raffinose, and stachyose. Significant genotypic differences (p < .001) among RILs were observed for sucrose, raffinose and stachyose contents across years. A 3038 Illumina SoyNAM BeadChip SNPs identified three QTLs for sucrose, one on chromosome 1, explaining 10% variance and two on chromosome 3 each explaining 22%. Raffinose QTL was detected on chromosome 6, explaining 6% variance. The mapped QTLs were novel and spanned regions harbouring candidate genes with roles in plant growth including seed development.     

Effect of root-exudates of Neem and Persian lilac on plant parasitic nematodes

Root-exudates of Neem/Margosa and Bakain/Persian lilac brought about considerable mortality ofHoplolaimus indicus, Helicotylenchus indicus, Tylenchus filiformis, Tylenchorhynchus brassicae, Rotylenchulus reniformis andMeloidogyne incognita, however, to varying extent. The root-exudate of Neem was more toxic to nematodes than that of Bakain. The root-exudates also inhibited larval hatch ofM. incognita.     

孟加拉吉大港地区乡村家庭竹手工业

对孟加拉吉大港地区家庭竹子手工业非农就业的潜力以及对其产生的社会经济效益进行了调查。本地区有40个竹企业,涉及到Hathazcori upazila的4个村庄。80%手工业者的年龄在30~50岁。他们的大多数是未受教育和土地贫瘠的家庭成员。50%的企业是由夫妻或3~5个工人组成的。竹产品主要是满足当地市场的需求。产品是根据季节的变化而决定的。5个产品中,竹书架提供了企业月净收入的41%,平均月净利润约2373.48塔卡,这为促进乡村经济发展起到重要的作用。作者对竹产业发展的状况进行了验证,并提出了建议。     

A general framework for the quantification and valuation of ecosystem services of tree-based intercropping systems

This study provides the first complete framework for the valuation of ecosystem services of agroforestry and uses a tree-based intercropping (TBI) system in southern Québec, Canada, as a case study. Ten ecosystem services were estimated, all of which were of interest and directly applicable to most agricultural systems worldwide: nutrient mineralization, water quality, soil quality, pollination, biological control, air quality, windbreak, timber provisioning, agriculture provisioning, and climate regulation. A mix of mathematical models for the quantification and economic valuation of various ecosystem services were used. The results revealed a total annual margin of $2,645 ha^?1 y^?1 (averaged over 40 years). The economic value of combined non-market services was $1,634 ha^?1 y^?1, which was higher than the value of marketable products (i.e. timber and agricultural products). An analysis of the present value suggested that agricultural products ranked highest among the ecosystem services taken singularly, followed by water quality, air quality, climate regulation, and soil quality maintenance. Total economic value of all ecosystem services for the rotation period was $54,782 ha^?1, only one third of which was contributed by agricultural products. Although the total value of the ecosystem services provided by TBI was high, farmers only benefited from agricultural and timber products. Thus, government incentives are needed to interest farmers in adopting practices that benefit society as a whole.     

Efficacy of nine insecticides against shoot and fruit borer, <Emphasis Type="Italic">Leucinodes orbonalis</Emphasis> Guenee (Lepidoptera: Pyralidae) in eggplant

Shoot and fruit borer, Leucinodes orbonalis Guenee, is a serious pest of eggplant (Solanum melongena L.). Management practices of this obnoxious pest are limited to frequent spray of chemical insecticides. Due to increasing levels of resistance of L. orbonalis to different insecticides there is an urgent need to test new chemicals. In this study, nine insecticides such as azadirachtin 0.03EC, abamectin 1.8EC, flubendiamide 24WG, chlorpyriphos 20EC, cartap 50SP, carbosulfan 20EC, thiodicarb 75WP, cypermethrin 10EC, and lambdacyhalothrin 2.5EC belonging to different chemical groups were tested against eggplant shoot and fruit borer in laboratory and field. In laboratory trial, carbosulfan and flubendiamide showed the highest toxicity against fourth instar larvae of L. orbonalis after 24 and 48 h of exposure, respectively. In field trials, they reduced more than 80% shoot and fruit infestation in winter, and 80% shoot and 70% fruit infestation in summer over control. Carbosulfan protected the highest amount of healthy fruit yield in both cropping seasons. Flubendiamide also showed the similar efficacy. Cartap and thiodicarb were moderately effective in both the seasons. Efficacy of cypermethrin and abamectin was moderate in winter but low in summer. Lambdacyhalothrin and chlorpyriphos although reduced shoot and fruit infestation of eggplant and protected higher yield as compared to control, their effectiveness was not satisfactory. The performance of azadirachtin against the pest both in the laboratory and field trials was the poorest while that of carbosulfan and flubendiamide was the best. Thus, it is suggested that carbosulfan and flubendiamide may be used for the control of L. orbonalis in eggplant.     

Impacts of initial stand density and thinning regimes on energy wood production and management-related CO<Subscript>2</Subscript> emissions in boreal ecosystems

An ecosystem model (Sima) was utilised to investigate the impact of forest management (by changing both the initial stand density and basal area thinning thresholds from current recommendations) on energy wood production (at energy wood thinning and final felling) and management-related carbon dioxide (CO₂) emissions for the energy wood production in Finnish boreal conditions (62°39′ N, 29°37′ E). The simultaneous effects of energy wood, timber and C stocks in the forest ecosystem (live and dead biomass) were also assessed. The analyses were carried out at stand level during a rotation period of 80 years for Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) growing in different fertility sites. Generally, the results showed that decreased basal area thinning thresholds, compared with current thinning, reduced energy wood (logging residues) and timber production, as well as carbon stocks in the forest ecosystem. Conversely, increased thinning thresholds increased energy wood production (ca. 1–27%) at both energy wood thinning and final felling and reduced CO₂ emissions (ca. 2–6%) related to the production chain (e.g. management operations), depending on the thinning threshold levels, initial stand density, species and site. Increased thinning thresholds also enhanced timber production and carbon stocks in the forest ecosystem. Additionally, increased initial stand density enhanced energy wood production for energy wood thinning for both species, but this reduced energy wood production at final felling for Scots pine and Norway spruce. This study concluded that increases in both initial stand density and thinning thresholds, compared with the current level, could be useful in energy wood, timber and carbon stocks enhancement, as well as reducing management-related CO₂ emissions for energy wood production. Only 2.4–3.3% of input of the produced energy (energy wood) was required during the whole production chain, depending on the management regime, species and sites. However, a comprehensive substitution analysis of wood-based energy, in respect to environmental benefits, would also require the inclusion of CO₂ emissions related to ecosystem processes (e.g. decomposition).     

Marker‐assisted introgression of rare allele of β‐carotene hydroxylase (crtRB1) gene into elite quality protein maize inbred for combining high lysine,tryptophan and provitamin A in maize

Vitamin A deficiency in humans is a widespread health problem. Quality protein maize (QPM) is a popular food rich in lysine and tryptophan, but poor in provitamin A (proA). Here, we report the improvement of an elite QPM inbred, HKI1128Q for proA using marker‐assisted introgression of crtRB1‐favourable allele. HKI1128 was one of the parental lines of three popular hybrids in India and was converted to QPM in our earlier programme. Severe segregation distortion for crtRB1 was observed in BC₁F₁, BC₂F₁ and BC₂F₂. Background selection by 100 SSRs revealed mean recovery of 91.07% recurrent parent genome varying from 88.78% to 93.88%. Across years, introgressed progenies possessed higher mean β‐carotene (BC: 9.22 µg/g), β‐cryptoxanthin (BCX: 3.05 µg/g) and provitamin A (proA: 10.75 µg/g) compared to HKI1128Q (BC: 2.26 µg/g, BCX: 2.26 µg/g and proA: 3.38 µg/g). High concentration of essential amino acids, viz. lysine (mean: 0.303%) and tryptophan (0.080%) in endosperm, was also retained. Multi‐year evaluation showed that introgressed progenies possessed similar grain yield (1,759–1,879 kg/ha) with HKI1128Q (1,778 kg/ha). Introgressed progenies with higher lysine, tryptophan and proA hold immense potential as donors and parents in developing biofortified hybrids.     

Analysis of genetic diversity and population structure of confectionery sunflower (<Emphasis Type="Italic">Helianthus annuus</Emphasis> L.) native to Iran

Genetic diversity within and among 50 populations of confectionery sunflower (Helianthus annuus L.) collected from different geographical areas of Iran was evaluated by using microsatellite and retrotransposon markers. The number of alleles (Na) in SSR loci ranged from 2 to 3 with an average of 2.1. The polymorphic bands in retrotransposon markers ranged from 7 in locus CR-UR1 to 15 in locus CR-816 with a mean value of 11.33. Herarchical clustering of individuals (50 × 5 = 250) by neighbor joining method in DARwin5 software subdivided them into three groups. Using Bayesian method in the software pakage of Structure, the studied individuals were subdivided into two sub-populations. Principal coordinate analysis revelaed that the two first components explaine 7.86 and 6.16% of the total variance, respectively. Analysis of molecular variance revealed a higher level of genetic variation within (70%) than between (30%) populations. High molecular variation among individuals within population possibly is due to high allogamy nature of the sunflower plant. Low genetic variation observed between populations could be considered as a consequence of genetic equilibrium that has occurred over the long period of cultivation of confectionery sunflower in this area as well as seed exchange among regions. The traditional assumption that selecting genotypes of different geographical origin will maximize the diversity available to a breeding project does not hold in confectionery sunflower.     

Maize yield response to deficit irrigation during low-sensitive growth stages and nitrogen rate under semi-arid climatic conditions

Knowledge of crop production in suboptimal environmental conditions not only helps to sustain crop production but also aids in the design of low-input systems. The objective of this study was to evaluate the effects of water stress imposed at low-sensitive growth stages (vegetative, reproductive, and both vegetative and reproductive) and level of nitrogen (N) supply (100 and 200 kg ha⁻¹) on the physiological and agronomic characteristics of two hybrids of maize (Zea mays L.). A two-site field experiment was carried out using a randomized complete block design with three replications and a split-factorial arrangement. A water deficit (WD) was induced by withholding irrigation at different stages of crop development. The results showed that proline content increased and the relative water content, leaf greenness, 100-kernel weight and grain yield decreased under conditions of WD. The highest IWUE was obtained when maize endured WD at vegetative stage at two sites. The limited irrigation imposed on maize during reproductive stage resulted in more yield reduction than that during vegetative stage, compared with fully irrigated treatment. The 100-kernel weight was the most sensitive yield component to determine the yield variation in maize plant when the WD treatments were imposed in low-sensitive growth stages. The results of the statistical regression analysis showed liner relationships between RGR during a period bracketing the V₈ or R₃ stages and 100-kernel weight in all the WD treatments. The increase of N supply improved yield and IWUE when maize plant endured once irrigation shortage at vegetative stage. But, the performance of high N fertilizer reduced and eliminated when water deficit imposed once at reproductive stage and twice at vegetative and reproductive stages, respectively. Furthermore, the response of T.C647 hybrid to increase of N supply was stronger than S.C647 hybrid.     

Copper,Chromium, and Arsenic Adsorption and Equilibrium Modeling in An Iron-Oxide-Coated Sand,Background Electrolyte System

The adsorption of copper, chromate, and arsenate (CCA)onto iron-oxide-coated sand (IOCS) was examined insingle-metal and mixed-metal systems. Copper andarsenate were strongly adsorbed or formed inner-spheresurface complexes with the IOCS surface while chromatewas weakly adsorbed or formed an outer-sphere surfacecomplex with the IOCS surface. Copper adsorption byIOCS slightly increased in the presence of arsenatebut was not affected by the presence of chromate. Arsenate adsorption was not affected by the presenceof copper and/or chromate. Chromate adsorptionincreased in the presence of copper by the combinationof electrostatic effects and possiblesurface-copper-chromate ternary complex formation. The presence of arsenate significantly decreasedchromate adsorption due to competition for adsorptionsites and electrostatic effects. Using inner-spheresurface complexation constants for copper and arsenateand outer-sphere surface complexation constants forchromate, the triple-layer model (TLM) was successfulin describing adsorption of copper, chromate, andarsenate in single-solute systems. The modelindicated that each metal uses a different number ofadsorption sites. For CCA metals-IOCS systems, theequilibrium constants determined from single-solutesystems were not able to predict adsorption frommulti-solute systems. The TLM does not currentlyaccount for the heterogeneity of oxide surface sitesand the formation of ternary complexes and/or solidphases that do not exist in single-solute systems.