Prevalence and infection pattern of Trypanosoma evansi in camels in mid-eastern Sudan

The antigen detection enzyme immunoassay (AgELISA) in conjunction with parasitological examination of blood were used to study the enzootic situation of cameline trypanosomiasis in mid-Eastern Sudan. A one year survey showed that the infection is endemic among pastoral camels with a prevalence of 5.4% based on parasitological examination and 31.3% based on AgELISA. The infection rate was higher during the dry period (November to May) than the wet season. Young camels had a much lower infection rate as detected by parasitological techniques, but not with AgELISA. A lower prevalence of infection was detected by buffy coat technique (BCT) in herds of camels raised by nomads compared with those kept by agropastoralists and in camels located in the southern districts of mid-Eastern Sudan.     

Pathogenicity of peste des petits ruminants virus isolated from Egyptian goats in Egypt

2 Egyptian goats and Boscat rabbits were experimentally inoculated with peste des petits ruminants (PPR) local Egyptian strain (PPR, Egypt 87). The inoculated animals contracted the disease with minor clinical manifestations, accompanied by rise of neutralizing antibodies to PPR virus. Virus was isolated from ocular and nasal secretions, buffy coat, spleen, and liver. No contact infection was observed between inoculated and healthy goats.     

A sequential broncho-alveolar washing in non-anaesthetized normal bovines: Method and preliminary results

The method of lung lavage under fiberoptic control allowed collection of alveolar cells in non-anaesthetized adult cows.The median section of the diaphragmatic lobe was lavaged with five consecutive aliquots of 30 ml each. Every one was analysed separately. A mean of 25.6% of instilled fluid was recovered and this is lower than amounts obtained on isolated lungs or in anaesthetized calves (about 50%).The cellular formula of 30 samples showed 83.5% of macrophages, 6.0% of lymphocytes, 9.4% of polymorphonuclear cells, 0.5% of monocytes. Cellular viability and total cell count were similar to previously published data. All results were found to be independent of the washing sequence. This simple and well tolerated technique appeared to be a useful tool for the study of defence mechanisms of deep lung.     

Biochemical and molecular effects of a commercial diuretic with herbal extract on experimentally induced urolithiasis in chickens

Veterinary Research Communications - This study evaluated the diuretic, antioxidant, anti-inflammatory, and immunological effects of a commercial diuretic (CD) (composed of ammonium chloride,...     

Early heat acclimation during incubation improves Japanese quail performance under summer conditions

Effects of exposing quail eggs to high temperature on the heat tolerance ability and productivity of birds were investigated. Four groups of 600 fertile eggs were randomly selected; the first group was incubated under 37.5 °C and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C (Control). The second group was exposed to 39.1 °C for 2 h/day during 4–14 days of embryogenesis and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C. The third group was incubated under 37.5 °C and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The fourth group was exposed to 39?±?1 °C for 2 h during 4–14 days of embryogenesis and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The temperature applied changed (P?<?0.01) embryo weight and incubation period. Birds exposed to high temperature during brooding had superior growth performance, dressed carcass, body temperature and health traits. Birds subjected to 39?±?1 °C during brooding exhibited decreased feed consumption and body weight gain. Finally, this work suggests that thermal acclimation during embryogenesis might offer a practical method for easing heat stress.

     

Chemical composition,antioxidant and antibacterial activities of extracts from <Emphasis Type="Italic">Schinus molle</Emphasis> wood branch growing in Egypt

In the present work, for the first time, the chemical components of essential oils (EOs) and extracts from wood branch (WB) resulted from the tree pruning wastes of Schinus molle L. grown in Egypt were evaluated for their antioxidant and antibacterial activities. EOs, methanol (ME), dichloromethane (DCME) and water (WE) extracts as antioxidant and antibacterial activities were measured. Total phenolic and flavonoid contents as well as analysis of extracts by gas chromatography–mass spectrometry (GC–MS) were reported. The major components in EOs were α-elemol, β-pinene, and α-phellandrene, in ME were 6-(4-chlorophenyl)-3-cyano-4-(N-benzylpiperazino)-2H-pyran-2-one, and 2-naphthalene methanol, decahydro-α,α,4a-trimethyl-8-methylene, in DCME were 12-methyl-E,E-3,13-octadecadien-1-ol, and 1,2-benzenedicarboxylic acid, dioctyl ester, and in WE were β-eudesmol, and (Z,Z,Z)-9,12,15-octadecatrienoic acid, 2,3-dihydroxypropyl ester. The highest total antioxidant activity was found with EOs (90 ± 1.23 %) and WE (86.30 ± 1.40 %). The lowest IC₅₀ values of 13.11 ± 3.00, and 12.66 ± 2.15 μg/mL were found with WE and EOs, respectively. EOs and WE were observed to have good antibacterial activity against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Sarcina lutea, Pseudomonas aeruginosa, and Micrococcus luteus. In conclusion, the Schinus molle L. WB EOs and extracts might, indeed, be used as a potential source for pharmaceutical or food industries.     

Über die molluscizide Wirkung vonBacillus thuringiensis-Präparaten zur biologischen Bekämpfung vonBiomphalaria alexandrina-Schnecken,den Zwischenwirten des Bilharziose-SaugwurmsSchistosoma mansoni in Saudi Arabia

The comparative molluscicidal activity of different preparations ofBacillus thuringiensis as Thuricide, SAN 401, SAN 402 and SAN 415, onB. alexandrina snails was studied. The results showed that different preparations ofBac. thuring. have a remarkable molluscicidal activity onB. alexandrina snails. However, the obtained results proved thatBac. thuring. with its preparation SAN 415 was the most effective in killing the snails comparing with the other preparations at the same experimental condition. Prolonged exposure ofB. alexandrina to subletal concentration (250 ppm) of SAN 415 delayed its ovipositary activity. Increasing the dose to 500 ppm resulted in a complete loss of egg production. Comparatively exposure of snail groups to the same doses of Thuricide, SAN 401 and SAN 402 resulted only in decreasing its egg laying capacity. These results proved the high potentially effectiveness ofBac. thuring. in its preparation SAN 415 as a biological control agent forB. alexandrina snails. The wayes by which this biological agent acts on the reproductive system of the snails were discussed.     

Bacteria wilt of Casuarina equisetifolia in India

Pseudomonas solanacearum (Smith) was consistently isolated from the wilted plants of Casuarina equisetifolia. The isolate gave positive reaction to oxidase, catalase and gas production from nitrate and negative reaction for arginine dihydrolase and acid was produced from various carbohydrates. Wilt produced on tomato, egg plant and capsicum indicated that the pathogen belongs to Race I and Biovar III of P. solanacearum which is a new record from India.     

Modeling the process of absorption and desorption of water above and below the fiber saturation point

Summary The process of absorption of water along the tangential direction of the wood is studied by immersing the sample in water. The transport of water is then obtained below the fiber saturation point at the beginning of the absorption and above this fiber saturation point during the process. The potential which drives the transport of the bound-water and free-water through the wood has been considered by testing a diffusional transport model. The transient diffusion with a constant diffusivity has been found to describe not only the process of absorption but also the process of desorption with diffusion of water through the solid and evaporation from the surface. Analytical solutions have been successfully used to describe the stage of absorption during a time of four hours at the end of which an equilibrium of absorption is attained, as well as the following stage of desorption. A model based on a numerical method with finite differences has been found to describe the process of absorption and desorption in various cases, and especially when the equilibrium of absorption has not been attained.This work was carried out with the help and support of the M. R. T. and the French CTB (Wood Technical Center)