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91.
酰基甘油酯酶(MAGL)是将酰基甘油分解为甘油和游离脂肪酸的丝氨酸水解酶家族成员之一,在酯代谢中起着关键酶的作用,是研制抗鸡球虫药物的重要靶标。本研究利用生物信息学技术预测拼接了柔嫩艾美耳球虫MAGL基因序列,以第二代裂殖子总RNA为模板,通过RTPCR技术获得Etmagl基因。将Etmagl与pCold-43a载体连接,构建pCold-43a-Etmagl重组载体,并在大肠杆菌BL21中获得可溶性蛋白,经亲和层析获得纯化的重组蛋白。结果显示,扩增的Etmagl序列ORF长1 752 bp,编码584个氨基酸,与预测序列相似度为99%,与弓形虫MAGL相似度好(55%);IPTG诱导后融合蛋白高效表达,大小约为114 ku,经免疫印迹鉴定为目的蛋白。本研究成功利用大肠埃希菌原核表达体系重组表达并纯化了柔嫩艾美耳球虫酰基甘油脂肪酶,为建立以MAGL为靶标的抗球虫药物筛选模型奠定了基础。 相似文献
92.
Minna Blomqvist Maiju Kosunen Mike Starr Tuula Kantola Markus Holopainen Päivi Lyytikäinen-Saarenmaa 《European Journal of Forest Research》2018,137(5):675-691
New measures for effective monitoring and controlling of bark beetle infestations are needed as a response to intensified outbreaks caused by the climate change. Various environmental factors affect tree health and susceptibility, as well as stand predisposition to bark beetles. European spruce bark beetle Ips typographus L. abundance and outbreak frequency in Finland has significantly increased during the last decade. The ability to identify sites under a high risk of infestation would facilitate adaptation to this new situation and help target limited forest health management resources. Accordingly, our goal was to investigate the importance of various stand, soil and topographic characteristics in the assessing predisposition of Norway spruce dominated urban forest in southern Finland to I. typographus infestations. Information on the environmental factors was assessed in the field in 2014 and derived from a digital elevation model. Ips typographus infestation intensity was classified into three infestation index classes based on tree-wise symptoms of resin flow, discoloration and defoliation. Cumulative logit link models were utilized for investigating stand-level infestation probability. The best explanatory factors were aspect, slope, site type and soil texture. Models with the highest cumulative probabilities for severe infestation were linked with eastern aspect, moderate steep slope and rich site type fertility (0.72) and eastern aspect, shallow soil and rich site type fertility (0.71). Higher soil C/N ratios with east aspect and rich site type fertility was associated with an increased risk of severe infestation in a third model. The lowest risk was associated with southern and southwestern aspects, fine soil texture, moderate site fertility and gentle slopes. 相似文献
93.
Roh-Johnson M Shemer G Higgins CD McClellan JH Werts AD Tulu US Gao L Betzig E Kiehart DP Goldstein B 《Science (New York, N.Y.)》2012,335(6073):1232-1235
Apical constriction changes cell shapes, driving critical morphogenetic events, including gastrulation in diverse organisms and neural tube closure in vertebrates. Apical constriction is thought to be triggered by contraction of apical actomyosin networks. We found that apical actomyosin contractions began before cell shape changes in both Caenorhabitis elegans and Drosophila. In C. elegans, actomyosin networks were initially dynamic, contracting and generating cortical tension without substantial shrinking of apical surfaces. Apical cell-cell contact zones and actomyosin only later moved increasingly in concert, with no detectable change in actomyosin dynamics or cortical tension. Thus, apical constriction appears to be triggered not by a change in cortical tension, but by dynamic linking of apical cell-cell contact zones to an already contractile apical cortex. 相似文献
94.
In order to study decay, and to improve the management and protection of old urban trees, a total of 256 felled urban trees were examined during 2001–2003: 95 Tilia spp., 74 Betula spp., and 87 Acer spp. Most of the trees (73%) were located in the main parks and along the main streets in the downtown area of Helsinki City, Finland. The mean age of the trees was over 60 years, and the majority (64%) were old park trees. Poor condition and increasing risk of failure were the main reasons for felling in 82% of the cases. Thirty three percent of these trees were degenerated or dead, but the amenity value of 14% of the risk trees was still high. The latter were old, big trees which posed a potential hazard, but had a vital and balanced crown.Some characteristic profiles for potential failure were identified for each of the tree species studied: Ganoderma lipsiense in the butts and hollows in the stems of Tilia spp., weak fork formations together with Rigidoporus populinus on Acer spp., and degeneration together with decay in the stem on Betula spp.Decay fungi most commonly identified were R. populinus, G. lipsiense, Inonotus obliquus and Piptoporus betulinus. In addition, Kretzschmaria deusta was very common in three of the parks, and on every one of the tree species investigated. 相似文献
95.
96.
In this study we introduce and test a new simple approach for estimating annual stand-level gross primary production (GPP), net primary production (NPP) and stem biomass growth based on carbon acquisition and allocation, by combining existing summary models. The focus is on the variation of GPP and NPP across different parts of Finland caused by climate. 相似文献
97.
98.
不同播期对紫花苜蓿生长性状及草产量的影响 总被引:1,自引:0,他引:1
2009年在内蒙古鄂尔多斯赛乌素嘎查,采用随机区组法进行了不同播期对紫花苜蓿生长性状及草产量影响的田间试验研究。连续2年对出苗时间、出苗率、越冬率、株高、物候期、草产量、鲜干比、茎叶比的测定结果表明,在春播与秋播区间内,出苗时间与播种日期存在显著的正相关关系,生育时期随播期的提前而相应提前;秋播越早,根颈越粗,入土越深,当年越冬性越强,翌年草产量也越高;不同播期对鲜干比、茎叶比影响甚微。综合评价认为,该地种植紫花苜蓿,宜选择秋季播种,阈值为9月中旬。 相似文献
99.
本试验进行了三批鸡的免疫研究。第一批试验检测不同类型新城疫疫苗在不同母源抗体水平(高和低)的鸡体上的免疫效果。结果显示,在高母源抗体时免疫,V4组和油乳剂灭活苗级抗体水平表现较低,而在低母源抗体时免疫,几种疫苗的抗体水平上升得很快,特别Lasota-克隆30组最明显,而油乳剂灭活苗组的抗体水平保持最长。根据此结果而制定了不同的免疫程序,进行了第二、第三批免疫试验,并分别在45天龄及70天龄时进行强 相似文献