Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.     

Identification,characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA(dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus(HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region(UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame(ORF) that encodes a deduced 4 867 amino acids(aa) polyprotein with three domains: RdRP, Hel and UGT(UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization(FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.     

优质国审棉品种——ZHM19

概述了ZHM19的选育过程、特征特性、产量、纤维品质和抗病性等特点及主要栽培技术措施。     

基于茎秆生物力学特性的油菜抗倒调控机制研究

为了应用生物力学特性指标对茎秆抗倒性进行评价，进而指导油菜抗倒栽培，以华杂62和金油杂158为试验材料，基于不同栽培因素下油菜茎秆的弹性模量、弯曲强度、剪切强度及抗倒性，分析了栽培因素对油菜茎秆力学特性指标的影响及其与抗倒性的相关性。结果表明：油菜抗倒性与茎秆的弹性模量、弯曲强度、剪切强度呈显著负相关；随着播期推迟，油菜茎秆的弹性模量、弯曲强度及剪切强度分别减小15.31%、29.16%和13.88%，倒伏指数增大32.18%；随着播种密度增大，油菜茎秆的弹性模量、弯曲强度及剪切强度均呈先增大后减小趋势，而倒伏指数先减小后增大；随着施氮量增加，油菜茎秆的弹性模量、弯曲强度及剪切强度均逐渐减小，而倒伏指数逐渐增大，与施氮量120 kg·hm^-2处理相比，在施氮量为360 kg·hm^-2下，油菜茎秆的弹性模量、弯曲强度及剪切强度分别减小39.43%、19.40%和16.63%，倒伏指数增大16.36%。因此，播期提前、适当增加播种密度、控制氮肥投入，有助于提高油菜茎秆的力学特性强度，从而增强油菜的抗倒伏能力。研究结果可为油菜抗倒栽培调控提供理论依据。     

广西田园综合体的建设现状和发展建议

文章通过对田园综合体的概念进行介绍，找出广西田园综合体发展情况和存在的问题，最后 提出解决广西田园综合体存在问题的对策和建议，从而促进广西农业的快速发展，带动当地经济稳步 提升。     

不同种植方式对甜菜产质量的影响

试验结果表明,在同等条件下,由于栽培方式不同,密度不同,块根产量、产糖量差异较大,以纸筒大垄双行为最好,甜菜块根产量、产糖量比对照小垄直播分别提高36.7%、39.8%,每公顷纯增效益1602元。其次是大垄双行覆膜,块根产量、产糖量比对照小垄直播分别提高31.5%、33.1%。     

导入AGPase基因的转基因可育水稻及其经济性状的研究

以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。     

古籍咨询工作中的新视角

本文讨论古籍咨询的服务创新问题,提出了内容咨询这一新概念。认为根据个人自身条件,有些咨询人员不妨可以对古籍的内容进行研究,这有利于与读者进行深入的交流,可以提高咨询水平的深度。     

农地租赁均衡价格影响因素分析

在一定的经济技术条件下,农地资源的合理配置和有效利用,取决于与之相关的一系列农地制度实施机制.文章基于产权理论、地租理论、土地供给与需求理论,以农地租赁均衡价格为切入点,对农地租赁的利益来源、均衡价格形成与演变影响因素进行了分析与探讨.结果认为,影响农地租赁均衡价格的因素主要是市场机制、政府机制以及习俗元制度.