Occurrence of two distinct Theileria lineages in sika deer (Cervus nippon) of Iwate Prefecture, Japan

In the present study, we tried to detect protozoan blood parasites from the liver or blood of 141 Japanese sika deer (Cervus nippon) in Iwate Prefecture of Japan by polymerase chain reaction. Approximately 500-bp amplicons were obtained in 76 (53.9%) of 141 samples by amplification for V4 hyper-variable regions of the 18S rRNA gene, and the amplicons were considered to be DNA of Theileria species. The complete nucleotide sequences (1701-bp) of the 18S rRNA gene were determined in 25 samples and were divided into 8 genotypes that were phylogenetically separated into two distinct lineages showing a monophyletic relation. The two lineages of Theileria were detected in different rates (12 and 88%) from sika deer in Iwate Prefecture.     

新疆冬小麦地方品种高分子量麦谷蛋白亚基的地理分布及其新发现亚基的特性

[目的]和方法]为了揭示新疆小麦地方品种高分子量谷蛋白亚基( HMW - GS)的遗传多样性,对新疆北部地区(北疆)、东部地区(东疆)和南部地区(南疆)小麦地方品种HMW - GS的分布进行了研究.[结果]研究表明:新疆小麦地方品种HMW - GS等位变异的分布与其地理来源具有密切关系,除Glu - B1位点外,Glu -A1和Glu - D1位点等位变异在北部地区、东部地区和南部地区的分布频率存在显著差异.在Glu - A1位点,Glu - A1c编码的亚基出现的频率最高,其次是Glu - A1b编码的2*亚基;但在北部地区,几乎所有品种都含有Glu -A1c编码的亚基,仅有1个品种含有Glu-A1b编码的2*亚基.在Glu - B1位点,新疆大多数小麦地方品种含有Glu -B1b编码的7+8亚基.在Glu - D1位点,新发现的等位基因Glu -D1bp(t)编码的2.6亚基在东疆和南疆出现的频率较高,但在北疆出现的频率最低,分别为91;(东疆),61;(南疆),19;(北疆);等位基因Glu - D1bp(t)在南疆冬小麦地方品种中普遍存在.然而,北疆是以等位基因Glu - D1a编码的2+12亚基为主,其频率为83;.关于Glu - D1bp(t)的起源,推测可能是在南疆地区自然突变产生,然而由于南疆和东疆荒漠化造成的地理阻碍使其向北疆传播的概率较低,同时抑制了该基因向东亚地区的迁移.[结论]基于DNA序列的氨基酸序列比对,发现Glu -D1bp(t)编码的2.6亚基与Glu - D1al编码的2.2*亚基非常相似.     

Anthracnose of <Emphasis Type="Italic">Enkianthus campanulatus</Emphasis> and <Emphasis Type="Italic">Rhynchosia acuminatifolia</Emphasis> caused by <Emphasis Type="Italic">Colletotrichum gloeosporioides</Emphasis> (new occurrence)

In 2003–2004, anthracnoses of Enkianthus campanulatus and Rhynchosia acuminatifolia were found for the first time in Kanagawa Prefecture and Tokyo in Japan. These pathogens were identified as Colletotrichum gloeosporioides based on their pathogenicity, morphology and ribosomal DNA spacer sequences. Results were presented at the annual meeting of The Phytopathological Society of Japan in 2004.