Estimating the outcrossing rate of Cyperus difformis using resistance to ALS-inhibiting herbicides and molecular markers

Cyperus difformis (smallflower umbrella sedge) is an economically important weed of rice in California where its control has recently been complicated by the evolution of herbicide resistance. Knowledge of the mating system of this weed is needed to elucidate the dynamics of resistance evolution and to design mitigation strategies that delay its occurrence. The aim of this study was to estimate the outcrossing rate of C. difformis using molecular and phenotypic markers. Outcrossing rates were estimated in natural field populations using sequence-related amplified polymorphism (SRAP) molecular markers and in glasshouse and field experiments using resistance to the acetolactate synthase-inhibiting herbicide bensulfuron-methyl as a phenotypic marker. Using SRAP markers, the multilocus ( t _m) and average single-locus ( t _s) outcrossing rates varied from 0.014 to 0.025 and from 0.008 to 0.012, respectively, among natural weed populations in rice fields. Using resistance to bensulfuron-methyl as a genetic marker, the average C. difformis outcrossing rate estimated was 0.009 in the glasshouse and 0.0084 in the field. These results indicate that C. difformis is a highly self-fertilising species. Therefore, the primary mechanism by which genes for herbicide resistance can be transferred among C. difformis populations in different rice fields is probably seed dispersal. Weed management should emphasise prevention of seed production and dispersal to preclude the further spread and evolution of resistance in C. difformis .     

Immunological detection of cardiac glycosides in plants

Summary Australian native and introduced plants were examined, using digoxin immunoassays, to detect the presence of cross-reacting cardiac glycosides. These compounds were found in 27 species from 20 genera. The assay technique can also be used on serum samples to confirm cardiac glycoside ingestion.     

A Markov model of land-use change dynamics in the Niagara Region,Ontario, Canada

Regional Niagara is the site of an intense three-way land-use conflict among urban, agricultural and natural uses. Large scale spatial and temporal land-use data were used to investigate the dynamics of land-use change in this area. A first order Markov chain was used as a stochastic model to make quantitative comparisons of the land-use changes between discrete time periods extending from 1935 to 1981. The Markov model allowed for two main conclusions about the historic dynamics of land-use change in the Regional Municipality of Niagara.

1. The urbanization of agricultural land was the predominant land-use change.
2. A continuing ‘exchange’ of land area occurs between wooded and agricultural land-use categories that has little effect on the net amount of wooded land but which could undermine the long-term ecological value of remaining natural areas in Niagara.

     

Tidal Currents and Eddy Statistics From Iceberg Trajectories off Labrador

Extensive data on the trajectories of icebergs located off the coast of Labrador has yielded information on tidal currents, the effect of wind on iceberg motion, and the properties of the low-frequency eddies. Statistical properties of the data were shown to be related to theoretical ideas on two-dimensional turbulence and to the connection between Eulerian and Lagrangian velocity statistics. The results have led to an estimate of the cross-shelf mixing rate and a statistical prediction scheme for iceberg trajectories.     

Transmissible spongiform encephalopathies in ruminants.

Transmissible spongiform encephalopathies (TSEs) have been observed in North American sheep, cervidae, and cattle. The causative agent of TSEs seems to be proteins that induce a conformational change in normal host proteins. Common clinical signs ofTSEs include chronic weight loss and deteriorating neurologic function. Mechanisms of TSE transmission vary among host species.The diagnostic approach for TSEs of ruminants is presented,and the history and justification of regulatory programs to control and eradicate TSEs are outlined.