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21.
Yojiro YANAGAWA Yukiko MATSUURA Masatsugu SUZUKI Shin-ichi SAGA Hideto OKUYAMA Daisuke FUKUI Gen BANDO Masashi NAGANO Seiji KATAGIRI Yoshiyuki TAKAHASHI Toshio TSUBOTA 《The Journal of reproduction and development》2015,61(1):61-66
Generally, sika deer conceive a single fetus, but approximately 80% of pregnant females have two corpora lutea (CLs). The function of the accessory CL (ACL) is unknown; moreover, the process of ACL formation is unclear, and understanding this is necessary to know its role. To elucidate the process of ACL formation, the ovarian dynamics of six adult Hokkaido sika deer females were examined ultrasonographically together with peripheral estradiol-17β and progesterone concentrations. ACLs formed in three females that conceived at the first estrus of the breeding season, but not in those females that conceived at the second estrus. After copulation, postconception ovulation of the dominant follicle of the first wave is induced by an increase in estradiol-17β, which leads to formation of an ACL. A relatively low concentration of progesterone after the first estrus of the breeding season is considered to be responsible for the increase in estradiol-17β after copulation. 相似文献
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Misa HOSOE Nao YOSHIDA Yutaka HASHIYADA Hidetoshi TERAMOTO Toru TAKAHASHI Sueo NIIMURA 《The Journal of reproduction and development》2014,60(4):268-273
Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in
vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum
replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the
rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%,
0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of
Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes
and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin
and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured
with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater
in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and
blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and
CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in
those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine
oocytes. 相似文献
27.
Weiping HUANG Masashi NAGANO Sung-Sik KANG Yojiro YANAGAWA Yoshiyuki TAKAHASHI 《The Journal of reproduction and development》2014,60(1):9-13
The objective of this study was to clarify the effects of prematurational culture
(pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic
maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95
μm in diameter) derived from early antral follicles (0.5–1 mm in diameter) were cultured
for 12 days for in vitro growth (IVG). IVG oocytes with a normal
appearance were subjected to examinations of diameter and chromatin structure in the
germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were
examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about
115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM
were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2,
the fertilizability and developmental competence of IVG oocytes were examined. Regardless
of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but
were lower than that of in vivo-grown oocytes (88.0%). Cleavage and
blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher
than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on
cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in
vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with
pre-IVM was lower than that of in vivo-grown oocytes. In conclusion,
pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes,
probably due to promotion of their chromatin transition and synchronization of meiotic
progression. 相似文献
28.
Noritaka ADACHI Daisuke YAMAGUCHI Akiyuki WATANABE Narumi MIURA Seiji SUNAGA Hitoshi OISHI Michiko HASHIMOTO Takatsugu OISHI Masaki IWAMOTO Hirofumi HANADA Masanori KUBO Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105
The objective of this study was to examine the health and meat production of cloned sows
and their progenies in order to demonstrate the application of somatic cell cloning to the
pig industry. This study compared the growth, reproductive performance, carcass
characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies.
We measured their body weight, growth rate and feed conversion and performed a
pathological analysis of their anatomy to detect abnormalities. Three of the five cloned
pigs were used for a growth test. Cloned pigs grew normally and had characteristics
similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a
Landrace boar and used for a progeny test. F1 progenies had characteristics similar to
those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White
boar and used for the progeny test. F2 progenies grew normally. There were no biological
differences in growth, carcass characteristics and amino acid composition among cloned
sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies
showed normal reproductive performance. No specific abnormalities were observed by
pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs
had a normal karyotype. These results demonstrate that cloned female pigs and their
progenies have similar growth, reproductive performance and carcass quality
characteristics and that somatic cell cloning could be a useful technique for conserving
superior pig breeds in conventional meat production. 相似文献
29.
Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160