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Érica Camila G. Praxedes Gabriela L. Lima Andréia M. da Silva Lívia B. Campos Carla Michelle P. de Souza Samara Sandy J. Moreira Moacir F. de Oliveira Alexandre R. Silva 《Reproduction in domestic animals》2020,55(2):154-161
In contributing to the conservation of wild rodents, the aim of this study was to evaluate the use of distinct cryoprotectants, separately or in combination, for solid surface vitrification (SSV) of red-rumped agouti ovarian tissue. Ovarian cortex from nine females was recovered and fragmented. Fresh fragments (control) were used to analyse the pre-antral follicle (PF) morphology using a histologic procedure, viability using the Trypan blue test, cell proliferation by counting the argyrophilic nucleolar organizing regions (Ag-NORs technique) and DNA integrity using the TUNEL assay. The remaining fragments were vitrified using SSV method with 3 M or 6 M ethylene glycol (EG) or dimethyl sulfoxide (DMSO), or in combination (3 M EG/3 M DMSO), and further evaluated as reported for the fresh samples. All cryoprotectants were effective at preserving PFs morphology compared to the control group (80.7 ± 5.21%), except 6 M EG and 3 M DMSO that provoked a significant (p < .05) decrease on the values of morphologically normal primary (60.0 ± 19.0%) and primordial (44 ± 4.5%) follicles, respectively. Regarding viability, all cryoprotectants provided values similar to that verified for the control group (79.0%), but a significant decrease (p < .05) was observed with EG/DMSO combination (59%). Using Ag-NORs technique, the highest (p < .05) cell proliferative capacity was detected when using EG at each tested concentration. The TUNEL proved the preservation of DNA integrity regardless of the cryoprotectant. In summary, we suggest the use of 3 M EG for the solid surface vitrification of red-rumped agouti ovarian tissue. 相似文献
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Lina Bernaola Molly Darlington Kadie Britt Patricia Prade Morgan Roth Adrian Pekarcik Michelle Boone Dylan Ricke Anh Tran Joanie King Kelly Carruthers Morgan Thompson John J Ternest Sarah E Anderson Scott W Gula Kayleigh C Hauri Jacob R Pecenka Sajjan Grover Heena Puri Surabhi Gupta Vakil 《Journal of insect science (Online)》2021,21(2)
The 2020 Student Debates of the Entomological Society of America (ESA) were live-streamed during the Virtual Annual Meeting to debate current, prominent entomological issues of interest to members. The Student Debates Subcommittee of the National ESA Student Affairs Committee coordinated the student efforts throughout the year and hosted the live event. This year, four unbiased introductory speakers provided background for each debate topic while four multi-university teams were each assigned a debate topic under the theme ‘Technological Advances to Address Current Issues in Entomology’. The two debate topics selected were as follows: 1) What is the best taxonomic approach to identify and classify insects? and 2) What is the best current technology to address the locust swarms worldwide? Unbiased introduction speakers and debate teams began preparing approximately six months before the live event. During the live event, teams shared their critical thinking and practiced communication skills by defending their positions on either taxonomical identification and classification of insects or managing the damaging outbreaks of locusts in crops. 相似文献
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Michelle T. Hulin Andrea Vadillo Dieguez Francesca Cossu Samantha Lynn Karen Russell Helen C. Neale Robert W. Jackson Dawn L. Arnold John W. Mansfield Richard J. Harrison 《Plant pathology》2022,71(4):949-965
Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental Prunus species and sweet cherry × ornamental cherry hybrids with the canker pathogens, Pseudomonas syringae pvs syringae, morsprunorum races 1 and 2, and avii. Several Prunus accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 P. syringae strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of P. syringae. Progeny resulting from a cross of a resistant ornamental species Prunus incisa with susceptible sweet cherry (P. avium) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm. 相似文献
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Kateřina Pánková Zbyněk Milec James Simmonds Michelle Leverington-Waite Lesley Fish John W. Snape 《Euphytica》2008,164(3):779-787
The single chromosome substitution lines of chromosome 3B of the Czech alternative wheat variety Česká Přesívka (CP 3B) into
two spring varieties Zlatka and Sandra, revealed clear differences in flowering time compared to the recipient varieties.
To map this gene(s), recombinant substitution lines for chromosome 3B were produced from crosses of the substitution lines
with their recipient parents and genetic maps developed using SSR markers. Two populations were mapped, Sandra//Sandra 3B/Sandra
(CP 3B) and Zlatka//Zlatka/Zlatka (CP 3B). Combining the genotype data with phenotype data on flowering time in five independent
experiments under natural long day or controlled short day conditions revealed a single flowering time QTL. This gene had
an additive effect of 1–6 days, depending on environment and genetic background, and was mapped in both populations to a position
in the region of marker Xbarc164 near the centromere on the long arm of 3B. Comparisons of the genetic maps with other 3B maps developed by the authors indicated
that the QTL may be homologous to a QTL segregating in UK germplasm. 相似文献