Bilateral aural hyalohyphomycosis in sable antelope (Hippotragus niger).

Three juvenile sable antelope (Hippotragus niger) were diagnosed with bilateral aural hyalohyphomycosis based on histopathology. All three animals were suspected to be immunodeficient based on low IgG levels determined using the zinc sulfate turbidity test. The serum and hepatic copper levels of one animal were below the bovine reference range. Clinical signs in the three animals included bilateral ventral deviation of the pinnae with multifocal subcutaneous aural tumefaction and poor body condition. Numerous septate, nonpigmented fungal hyphae were found within the auricular cartilage, dermis, and subcutaneous granulomas. No significant fungal agents were isolated by culture, and no signs of systemic fungal dissemination were identified except for a concurrent fungal rhinitis in one animal.     

Changes in the competence of Quercus robur ‘Fastigiata’ to grow in vitro as affected by seedling rootstocks and differential pruning

Three physiologically distinct forms of the same genotype of Quercus robur ‘Fastigiata’, all derived from adult budwood, were identified following the growth of severely-pruned hedge plants grafted onto juvenile seedling rootstocks. Only explants from the form displaying the juvenile-like characteristic of over-winter leaf retention were capable of establishing sustainable cultures in vitro, although growth was not as rapid as those from a two year old seedling source. Pruning to release correlative inhibition of buds low on the scion framework produced vigorous shoot growth, but explants from these failed to show sustained growth in vitro. Withholding light from stockplants reduced excessive phenolic oxidation in explants taken from flushing unripe shoots, however, despite a beneficial effect on culture initiation, later growth was dependent upon the juvenile- or adult-like physiology of the original explant. A new medium was devised for this subject, which in addition to promoting growth generally, also stimulated the flushing of shoot apices. The relevance to in vitro culture of expression of juvenile habit in the stockplant is discussed.     

Preliminary evaluation of diagnostic tests using horses experimentally infected with trypanosoma evansi

Seven surra negative horses were intravenously inoculated with 3 x 10(6)Trypanosoma evansi parasites derived from a camel. One horse was maintained as an uninfected negative control. Three antigen and three antibody detection tests were evaluated for diagnosis of infection in horses. The microhaematocrit centrifugation test (MHCT) was the most sensitive, first detecting parasites between one and three days (x 2.4) post infection (p.i.). The antigen (ag)-ELISA detected antigen between three and ten days (x 6.6) p.i. The latex agglutination test (LAT) first gave positive results on day 3 (x 3.0) p.i. Following the treatment of horses with trypanocidal drugs, the MCHT and the mouse inoculation test (MIT) became negative. Antigen levels using LAT declined and reached pre-infection levels in five out of six horses during the period of observation (92-279 days). Antigen levels using the ag-ELISA declined as well but did not reach pre-infection levels in any of the six horses.Three antibody detection techniques, ab-ELISA, card agglutination test (CATT), and immunofluorescent antibody test (IFAT) detected antibodies in the blood of all seven infected horses but not in the uninfected control. However, the ab-ELISA did not discriminate clearly between sera from infected and uninfected horses because unacceptably high ELISA background readings were detected in 15% of the surra negative horses shipped to the UAE from the UK. The ELISA antibody increased above pre-infection levels in the six horses experimentally infected, but not in one horse. In this horse the ELISA antibody level exceeded the cut-off level only after the reoccurrence of the T. evansi infection. The IFAT detected antibodies 15.7 days p.i. in all infected horses.     

Egg production responses of hens given a complete mash or unground grain together with concentrate pellets

Thirty‐three light hybrids aged 11 months were caged individually and fed for S weeks according to three regimes, viz. A, a conventional mash ad libitum; B, a mixture of whole wheat, barley and kibbled maize ad libitum in the morning, with concentrate pellets ad libitum in the afternoon; or C, ad libitum the same grain mixture together with concentrate pellets and oystershell grit in the ratio 70 : 23 : 7 so that the nutritive value of the complete diet was similar to that of the conventional mash. The concentrate pellets used in the two regimes differed. Those used in regime B contained ground limestone while those used in regime C did not.

Egg production was similar on the three regimes (74.8, 74.7 and 74.5% respectively) . Food intake was lowest on regime C and highest on regime B, although the intakes of metabolisable energy were very similar on the three regimes. Compared with the results obtained with the conventional mash diet (regime A), the conversions of food and dietary protein to eggs were lower on regime B but higher on regime C.     

INVESTIGATION OF EQUINE INFECTIOUS ANAEMIA IN QUEENSLAND USING GEL DIFFUSION

An antigen for the gel diffusion test for equine infectious anaemia (EIA) was prepared from the spleen of a horse experimentally infected with the CQ strain of the virus. The antigen produced a single, distinct line of precipitation when tested against a range of known positive serums, and did not react with pre-inoculation and known negative serums. Extracts prepared from uninfected spleens displayed no reaction when similarly tested. Serum from 34 of 451 Queensland horses contained detectable levels of antibody to EIA virus. The positive serums were from horses in widely separated areas of the State.     

Prevalence and risk factors for anti-Toxoplasma gondii antibodies in goats of the Seridó Oriental microregion, Rio Grande do Norte state, Northeast region of Brazil

The prevalence and risk factors for anti-Toxoplasma gondii antibodies were investigated in goats of the Seridó Oriental microregion, Rio Grande do Norte state, Northeast region of Brazil. Three hundred and sixty-six blood samples from goats collected by jugular venopuncture were used. For the serologic diagnosis of Toxoplasma gondii infection, the indirect fluorescent-antibody test (IFAT) with cut-off value 1:64 was carried out. The prevalence of anti-T. gondii antibodies was 30.6% [95% CI=25.9-35.6%] with titers ranging from 1:64 to 1:16,384. The multivariate logistic regression analysis showed that the risk factors associated to anti-T. gondii antibodies were presence of cats in the herd, extensive/semi-intensive management systems and lack of mineral supplementation.