全文获取类型
收费全文 | 446篇 |
免费 | 35篇 |
专业分类
林业 | 26篇 |
农学 | 7篇 |
基础科学 | 3篇 |
70篇 | |
综合类 | 36篇 |
农作物 | 18篇 |
水产渔业 | 38篇 |
畜牧兽医 | 251篇 |
园艺 | 8篇 |
植物保护 | 24篇 |
出版年
2024年 | 2篇 |
2023年 | 6篇 |
2022年 | 12篇 |
2021年 | 17篇 |
2020年 | 16篇 |
2019年 | 22篇 |
2018年 | 25篇 |
2017年 | 16篇 |
2016年 | 13篇 |
2015年 | 12篇 |
2014年 | 20篇 |
2013年 | 26篇 |
2012年 | 32篇 |
2011年 | 32篇 |
2010年 | 20篇 |
2009年 | 15篇 |
2008年 | 26篇 |
2007年 | 31篇 |
2006年 | 20篇 |
2005年 | 26篇 |
2004年 | 24篇 |
2003年 | 25篇 |
2002年 | 10篇 |
2001年 | 11篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1988年 | 1篇 |
1984年 | 2篇 |
1981年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有481条查询结果,搜索用时 15 毫秒
41.
David J. Burke Shun Zhu Michele P. Pablico-Lansigan Charlotte R. Hewins Anna Cristina S. Samia 《Biology and Fertility of Soils》2014,50(7):1169-1173
We examined the effect of TiO2 nanoparticles (NPs) on the growth of maize and soybean plants and associated soil microbial communities. Plants were grown in a greenhouse, and low levels of undoped or nitrogen-doped TiO2 NPs were applied. Plant growth and nutrient content were determined, and effects of NPs on composition of soil microbial communities were examined using terminal restriction fragment length polymorphism analysis (TRFLP) of rDNA. We found no significant effects of TiO2 NPs on plant growth, nutrient content, or the composition of bacterial communities within the rhizosphere. However, arbuscular mycorrhizal fungal communities were affected by application of undoped and nitrogen-doped TiO2 NPs. This observation may be partially attributed to the small but significant TiO2 NP uptake levels in the root tissues of both plants. Our results suggest that even low concentrations of TiO2 NPs may influence some important groups of soil microbes, such as mycorrhizal fungi, but changes in the composition of microbial communities may not affect plant growth under conditions of adequate moisture and nutrients. 相似文献
42.
Michele R. Derrick Jarvis L. Moyers Keith A. Yarborough Mike Warren 《Water, air, and soil pollution》1984,21(1-4):171-181
Aerosol chemistry, precipitation and visibility parameters are currently being measured at Big Bend National Park in Texas. This is part of a large-scale air resource evaluation program which the National Park Service is sponsoring in several southwestern national parks and monuments to determine the potential impact of local and distant pollutant sources on the environmental quality within these areas. Analysis of aerosol samples collected at six sites in the Southwest indicates that soil-derived components, organic materials and the acid-base ions of sulfate, nitrate, and ammonium are the major constituents of suspended airborne particulate matter in the remote areas of the arid region. Comparison of particulate matter chemistry and precipitation chemistry data at Big Bend National Park shows consistent features which indicate that the airborne alkaline soil material and NH3 largely neutralize the atmospheric acidic species of H2SO4 and HNO3. Given the similarity of the particulate matter composition and loading at the other monitoring sites, it is suggested that the trace chemical composition of precipitation will be similar in many remote regions of the Southwest. 相似文献
43.
Arienzo Michele Adamo Paola Rosaria Bianco Maria Violante Pietro 《Water, air, and soil pollution》2001,131(1-4):349-366
A study was conducted to determine the contaminantlevel of the Sarno River basin, a small creek located insouthwestern Italy. Six sampling site locations were selected tocoincide with two previous studies conducted in 1975 and 1985 toprovide a basis for comparison. For each location, twelve sampleswere collected over the period October 1997–September 1998.Results indicated a degradation in river water quality, especiallynear the mouth. This was reflected by a dissolved oxygenconcentration of about 0.8 mg L-1 near the urban areas ofScafati, Pompei and Castellamare of Stabia. At the same locations,BOD5 and COD values were two to five times higher than theallowable regulatory maximum levels, respectively. About 4 kmfrom the headwaters, the average oxygen concentration was 4.7 mgL-1, BOD5 values were close to the standard limit (37 vs 40 mg L-1), and COD values were above the standard limits(252 vs 160 mg L-1). Highest concentrations were detectedduring peak tomato production. During this time, settleable solidswere also elevated, two to six times the regulatory standard.Coliform bacteria densities were found in excess of the standardlimits at all sites. Near the head of the river, mean fecalcoliform (22,571 MPN mL-1) and streptococci densities (14,214 MPN mL-1) surpassed the regulatory level of 120 and 20 MPN mL-1, respectively, reflecting the input from the urbansettings of Sarno and agricultural fields. The heavy metaldissolved concentrations were low at all sampling sites. Most ofthe analyzed contaminants increased when comparend with 1985data. BOD5 increased 5 to 10 times at sites near the urbansettings of Scafati, Pompei and Castellamare di Stabia, whereasCOD increases were twofold at all sites. A decline of chromiumlevels was observed due to the recent effective treatment ofwaste water from the tanning plants. 相似文献
44.
Srgio H. G. SILVA David C. WEINDORF Wilson M. FARIA Leandro C. PINTO Michele D. MENEZES Luiz R. G. GUILHERME Nilton CURI 《土壤圈》2021,31(4):615-626
Portable X-ray fluorescence (pXRF) spectrometry and magnetic susceptibility (MS) via magnetometer have been increasingly used with terrain variables for digital soil mapping. However, this methodology is still emerging in many countries with tropical soils. The objective of this study was to use proximal soil sensor data associated with terrain variables at varying spatial resolutions to predict soil classes using the Random Forest (RF) algorithm. The study was conducted on a 316-ha area featuring highly variable soil classes and complex soil-landscape relationships in Minas Gerais State, Brazil. The overall accuracy and Kappa index were evaluated using soils that were classified at 118 sites, with 90 being used for modeling and 28 for validation. Digital elevation models (DEMs) were created at 5-, 10-, 20-, and 30-m resolutions using contour lines from two sources. The resulting DEMs were processed to generate 12 terrain variables. Total Fe, Ti, and SiO2 contents were obtained using pXRF, with MS determined via a magnetometer. Soil class prediction was performed using the RF algorithm. The quality of the soil maps improved when using only the five most important covariates and combining proximal sensor data with terrain variables at different spatial resolutions. The finest spatial resolution did not always provide the most accurate maps. The high soil complexity in the area prevented highly accurate predictions. The most important variables influencing the soil mapping were MS, Fe, and Ti. Proximal sensor data associated with terrain information were successfully used to map Brazilian soils at variable spatial resolutions. 相似文献
45.
Bertoli A Giovannini A Ruffoni B Guardo AD Spinelli G Mazzetti M Pistelli L 《Journal of agricultural and food chemistry》2008,56(13):5078-5082
A wild strain of Agrobacterium rhizogenes was used to regenerate twelve in vitro plant lines from different hairy roots of H. perforatum (St. John's Wort). The production of the main bioactive constituents was observed even though their yields varied in the different plant lines. Two lines were selected for the hyperoside production (4.9-4.6 mg/gdw) while nine were characterized by significant yields of chlorogenic acid (ranged from 0.47 to 1.09 mg/gdw). Furthermore, one out of twelve lines showed a 10-fold higher hypericin content (0.25 mg/gdw) than that reported for the in vitro shoots in the literature. Morphological and phytochemical features were determined in order to select H. perforatum genotypes enriched in valuable bioactive compounds. 相似文献
46.
Gómez-Caravaca AM Cerretani L Bendini A Segura-Carretero A Fernández-Gutiérrez A Del Carlo M Compagnone D Cichelli A 《Journal of agricultural and food chemistry》2008,56(12):4577-4583
The phenolic fraction of virgin olive oil influences both its quality and oxidative stability. One of the principal threats of the quality of olive fruit is the olive fly ( Bactrocera oleae) as it alters the chemical composition. The attack of this olive pest has been studied in order to evaluate its influence on the quality of virgin olive oil (free acidity, peroxide value, fatty acid composition, water content, oxidative stability, phenols, and antioxidant power of phenolic fraction). The study was performed using several virgin olive oils obtained from olives with different degrees of fly infestation. They were acquired in different Italian industrial mills from the Abruzzo region. Qualitative and quantitative analyses of phenolic profiles were performed by capillary electrophoresis-diode array detection, and electrochemical evaluation of the antioxidant power of the phenolic fraction was also carried out. These analyses demonstrated that the degree of fly attack was positively correlated with free acidity ( r = 0.77, p < 0.05) and oxidized products ( r = 0.58, p < 0.05), and negatively related to the oxidative stability index ( r = -0.54, p < 0.05) and phenolic content ( r = -0.50, p < 0.05), mainly with secoiridoid compounds. However, it has been confirmed that the phenolic fraction of olive oil depends on several parameters and that a clear correlation does not exist between the percentages of fly attack and phenolic content. 相似文献
47.
Francesco Carimi Francesco Mercati Roberto De Michele Maria Carola Fiore Paolo Riccardi Francesco Sunseri 《Genetic Resources and Crop Evolution》2011,58(7):967-975
The Sicilian grape cultivar ‘Nero d’Avola’ is among the oldest and most cultivated in the island, taking part in the production
of several red wines exported worldwide, including DOC wines (Etna Rosso and Cerasuolo di Vittoria). Due to the ancient origin
and repeated clonally propagation of the cultivar, phenotypic variability has been observed. Clone identification in this
important cultivar has so far relied on phenotypic and chemical traits analyses, often affected by environmental conditions.
Genetic markers, such as microsatellites, are particularly useful for cultivar identification, parentage testing, pedigree
reconstruction and population structure studies. In the present paper, microsatellites were used to analyze the intra-varietal
genetic diversity among 118 plants of ‘Nero d’Avola’, collected in 30 vineyards displaced in different areas of Sicily. Out
of 22 microsatellites, 11 showed polymorphism among samples and 15 different phylogenetic groups were identified. Results
show that ‘Nero d’Avola’ actually comprises different genetic profiles, although most of clones share a common origin. 相似文献
48.
Soil chemical and biochemical properties of a salt-marsh alluvial Spanish area after long-term reclamation 总被引:2,自引:0,他引:2
Vito Armando Laudicina Maria Dolores Hurtado Luigi Badalucco Antonio Delgado Eristanna Palazzolo Michele Panno 《Biology and Fertility of Soils》2009,45(7):691-700
Marisma, one of the largest salt-marsh alluvial areas in SW Spain, has been reclaimed since 1970 by artificial drainage and
amendment with phosphogypsum (PG) so as to reduce Na+ saturation. Within the reclaimed area, two 250- × 20-m plots were treated as follows: (1) amendment with 25 Mg/ha of PG every
2 to 3 years between 1979 and 2003 (plot PY); (2) like PY but PG treatment stopped after 1997 (plot DR). A contiguous virgin
Marisma salt-marsh plot (MV), neither drained nor amended, was the control. In MV, soil microbial biomass C, most enzyme activities
and total organic C content were much greater than in PY and DR soils, despite the salinity stress. The decrease in soil organic
matter content in PY and DR soils was likely due to cotton-cropping practices, which favoured the organic C mineralisation
and nutrients removal by crops. Microbial activity of MV soil, probably stimulated by the rhizodepositions of the natural
vegetation, did not suffer from the osmotic effect due to the raising of soil solution ionic strength. Microbial quotient
could be ranked as MV > PY > DR, whereas the metabolic quotient had an opposite trend. Thus, both quotients suggested that
the interruption of PG amendment was not favouring microbial activity. Principal component analysis clearly identified the
chemical and biochemical soil properties mostly affected by the reclamation and also indicated the longer PG amendment in
PY plot. Stepwise discriminant analysis identified two physiologically different types of soil microflora, one less active
present in the MV virgin soil and another more active present in the reclaimed PY and DR soils. 相似文献
49.
Longobardi F Solfrizzo M Compagnone D Del Carlo M Visconti A 《Journal of agricultural and food chemistry》2005,53(24):9389-9394
Two methods for the determination of dichlorvos in durum wheat by electrochemical assay and gas chromatography, respectively, have been developed. Dichlorvos, an organophosphorus anticholinesterase pesticide, was extracted from wheat with hexane, and the filtered extract was directly analyzed by gas chromatography with nitrogen-phosphorus flame detection (NPD). Recoveries of dichlorvos from milled wheat spiked at 0.25-1.5 microg/g ranged from 96.5 to 100.9%, and the limit of detection was 0.02 microg/g. The electrochemical assay was based on the detection of choline, the acetylcholinesterase product, via a choline oxidase biosensor. An aliquot of the filtered hexane extract was partitioned with phosphate buffer solution, and the organic layer was evaporated prior to electrochemical analysis. A limit of detection of 0.05 microg/g of dichlorvos was obtained with mean recoveries of 97-103% at spiking levels of 0.25-1.5 microg/g. A good correlation (r = 0.9919) was found between the results obtained with the electrochemical and those obtained with the gas chromatographic methods. The electrochemical method was peer-validated in two laboratories that analyzed 10 blind samples (5 duplicates), including a blank and 4 spiked samples with dichlorvos at levels of 0.25, 0.60, 1.00, and 1.50 microg/g. Within-laboratory repeatability (RSDr) and between-laboratory reproducibility (RSDR) ranged from 5.5 to 7.8% and from 9.9 to 17.6%, respectively. 相似文献
50.
Stability and reliability of yield and quality for 15 durum wheat genotypes (old and modern) were evaluated in a 5‐year experiment (southern Italy) in organic farming. Genotypes were grown at two N levels (0 and 80 kg/ha), with the aim of evaluating ‘genotype × environment’ (GE) interactions and their role on genotype selection in N‐limited environments. Several approaches to stability were used, within the frame of mixed models and additive main effects and multiplicative interaction analysis, and their validity in stressful conditions and organic farming was discussed. Especially for protein and gluten content, results indicate high environmental variability and the presence of crossover ‘N × environment’ interactions, which supports the need for specific breeding programmes in N‐deficient environments. The average response was strongly affected by N availability (on average, yield was 2.95 and 3.42 t/ha, protein content was 11.6% and 12.85%, gluten content was 8.55% and 9.92%, respectively, at 0 and 80 kg N/ha), and few genotypes gave high yield and quality at both fertilization levels. Only ‘Gargano’ and ‘Fortore’ showed a good inter‐year stability at both N levels. The old cultivars gave minimal responsiveness to increased N input, but gave good results in limiting conditions, indicating that they may play a role in organic farming. 相似文献