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The concept of foetal programming(FP) originated from human epidemiological studies, where foetal life nutrition was linked to health and disease status later in life. Since the proposal of this phenomenon, it has been evaluated in various animal models to gain further insights into the mechanisms underlying the foetal origins of health and disease in humans. In FP research, the sheep has been quite extensively used as a model for humans. In this paper we will review findings mainly from our Copenhagen sheep model, on the implications of late gestation malnutrition for growth, development, and metabolic and endocrine functions later in life, and discuss how these implications may depend on the diet fed to the animal in early postnatal life. Our results have indicated that negative implications of foetal malnutrition, both as a result of overnutrition and, particularly, late gestation undernutrition, can impair a wide range of endocrine functions regulating growth and presumably also reproductive traits. These implications are not readily observable early in postnatal life, but are increasingly manifested as the animal approaches adulthood. No intervention or cure is known that can reverse this programming in postnatal life. Our findings suggest that close to normal growth and slaughter results can be obtained at least until puberty in animals which have undergone adverse programming in foetal life, but manifestation of programming effects becomes increasingly evident in adult animals.Due to the risk of transfer of the adverse programming effects to future generations, it is therefore recommended that animals that are suspected to have undergone adverse FP are not used for reproduction. Unfortunately, no reliable biomarkers have as yet been identified that allow accurate identification of adversely programmed offspring at birth,except for very low or high birth weights, and, in pigs, characteristic changes in head shape(dolphin head). Future efforts should be therefore dedicated to identify reliable biomarkers and evaluate their effectiveness for alleviation/reversal of the adverse programming in postnatal life. Our sheep studies have shown that the adverse impacts of an extreme, high-fat diet in early postnatal life, but not prenatal undernutrition, can be largely reversed by dietary correction later in life. Thus, birth(at term) appears to be a critical set point for permanent programming in animals born precocial,such as sheep. Appropriate attention to the nutrition of the late pregnant dam should therefore be a priority in animal production systems. 相似文献
13.
Flemming Ekelund Stephane Saj Mette Vestergrd Joanne Bertaux Juha Mikola 《Soil biology & biochemistry》2009,41(11):2336-2342
Protozoa stimulate plant growth, but we do not completely understand the underlying mechanisms, and different hypotheses seek to explain this phenomenon. To test these hypotheses, we grew the grass Yorkshire fog (Holcus lanatus) in pots with soil, which contained either (1) no organisms but bacteria – or (2) bacteria and protozoa. Half of the pots received a glucose treatment so as to mimic an additional root exudation. We measured plant growth and plant nitrogen uptake, along with various microbial pools and processes that support plant growth. Protozoan presence significantly enhanced soil nitrogen mineralization, plant nitrogen uptake from organic nitrogen sources, plant nitrogen content, and plant growth. By contrast, we found no evidence that glucose addition, mimicking root exudation, increased soil nitrogen availability and plant nitrogen uptake. Moreover, although protozoan presence affected bacterial community structure, it did not affect the proportion of IAA-producing bacteria in the community or plant root morphology. These results refute the “soil microbial loop” hypotheses, which suggest that protozoan stimulation of plant growth results from complex interactions between plant roots, bacteria and protozoa. Our experiment thus favours the simple explanation that increased nitrogen availability is the key factor behind the positive protozoan effect on plant growth. To exploit natural resources in an efficient and environmentally friendly way, we need to understand in detail the functioning of ecosystems. This study stresses that to achieve this, it is still urgent, besides investigating intricate food-web and signal compound interactions, also to focus on the basic stoichiometric and energetic aspects of organisms. 相似文献
14.
Susanne Brandl Tobias Mette Wolfgang Falk Patrick Vallet Thomas Rötzer Hans Pretzsch 《Annals of Forest Science》2018,75(2):56
Key message
Static site indices determined from stands’ top height are derived from different forest inventory sources with height and age information and thus enable comparisons and modeling of a species’ productivity encompassing large environmental gradients.Context
Estimating forest site productivity under changing climate requires models that cover a wide range of site conditions. To exploit different inventory sources, we need harmonized measures and procedures for the productive potential. Static site indices (SI) appear to be a good choice.Aims
We propose a method to derive static site indices for different inventory designs and apply it to six tree species of the German and French National Forest Inventory (NFI). For Norway spruce and European beech, the climate dependency of SI is modeled in order to estimate trends in productivity due to climate change.Methods
Height and age measures are determined from the top diameters of a species at a given site. The SI is determined for a reference age of 100 years.Results
The top height proves as a stable height measure that can be derived harmoniously from German and French NFI. The boundaries of the age-height frame are well described by the Chapman-Richards function. For spruce and beech, generalized additive models of the SI against simple climate variables lead to stable and plausible model behavior.Conclusion
The introduced methodology permits a harmonized quantification of forest site productivity by static site indices. Predicting productivity in dependence on climate illustrates the benefits of combined datasets.15.
Blandine Marie Ivette Nacoulma Anne Mette Lykke Salifou Traoré Brice Sinsin Adjima Thiombiano 《Agroforestry Systems》2017,91(3):565-576
In sub-Saharan Africa, extraction for daily livelihood needs often results in uncontrolled exploitation of bark and leaves of valuable medicinal and fodder trees. However, overharvesting of bark and foliage can reduce fruit production and threaten reproduction. This study evaluates the impact of combined bark and foliage harvesting on the performance of fruit production of Afzelia africana in Burkina Faso. We compared fruit and seed production at different harvesting intensities. Data on fruit yields were collected by stratified random sampling of 91 trees with no, low, severe, and very severe harvesting intensities. The fruit production varied with harvesting intensity, tree size and number of branches. Fruit and seed quantity and quality decreased with increasing harvesting intensity. However, no significant difference was detected between trees without and trees under low harvesting. Trees of all size classes under very severe harvesting intensity had no fruits. Under low harvesting impact, large trees had twice as many fruits as the control, whereas fruits were reduced by half to 95 % for the small trees. High harvesting intensity is an unsustainable practice that should be completely prohibited in order to ensure longterm persistence of Afzelia africana. Low harvesting intensity should be allowed, but only on large reproductive individuals. 相似文献
16.
BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only partial and indicated possible target‐site resistance. The nAChR α2 subunit is important in neonicotinoid toxicity to insects, and gene expression of the Mdα2 subunit was investigated in field populations and laboratory strains of neonicotinoid‐resistant and insecticide‐susceptible houseflies, Musca domestica L. The genomic sequence covering exon III–VII of Mdα2 was analysed for mutations. RESULTS: Gene expression profiling of Mdα2 revealed notable differences between neonicotinoid‐resistant and insecticide‐susceptible houseflies. On average, the neonicotinoid‐resistant field population 766b and the imidacloprid selected strain 791imi had 60% lower copy numbers of Mdα2 compared with the susceptible reference strain. Sequencing of exon III–VII of the Mdα2, encoding acetylcholine binding‐site regions and three out of four transmembrane domains, did not reveal any mutations explaining the increased neonicotinoid tolerance in the strains examined. CONCLUSION: Previous discoveries and the results of this study suggest that the neonicotinoid resistance mechanism in Danish houseflies involves both cytochrome P450 monooxygenase‐mediated detoxification and reduced expression of the nAChR subunit α2. Copyright © 2010 Society of Chemical Industry 相似文献
17.
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are
difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional
analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis
with arbuscular mycorrhizal fungi (AMF). 相似文献
18.
Andrzej Pacak Katrin Geisler Bodil Jørgensen Maria Barciszewska-Pacak Lena Nilsson Tom Hamborg Nielsen Elisabeth Johansen Mette Grønlund Iver Jakobsen Merete Albrechtsen 《Plant methods》2010,6(1):26
Background
Gene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited to genes expressed in leaves of barley and wheat. However since many important aspects of plant biology are based on root-expressed genes we wanted to explore the potential of BSMV for silencing genes in root tissues. Furthermore, the newly completed genome sequence of the emerging cereal model species Brachypodium distachyon as well as the increasing amount of EST sequence information available for oat (Avena species) have created a need for tools to study gene function in these species. 相似文献19.
20.
Paratuberculosis was diagnosed in a goat herd that participated in a sanitation program against Mycobacterium avium subsp. paratuberculosis. The aim of this study was to characterise the development of gamma interferon (IFN-γ) and antibody responses as well as the occurrence of faecal shedding. Faecal culture appeared surprisingly sensitive as about 18% and 40% of the goats were positive at 9 and 15-17 months of age, respectively, and shedding was often seen prior to peripheral immune responses. Peripheral IFN-γ responses were not related to protection as clinical and high shedding goats often had high responses. An IFN-γ response usually preceded a humoral response. However, positive antibody titers could sometimes be seen simultaneously with, and even prior to, IFN-γ responses. In conclusion, faecal culture appeared as sensitive as IFN-γ testing. Furthermore, the antibody ELISA and the IFN-γ assay may perform equally well in an infected herd if surveillance is conducted annually. 相似文献