口蹄疫加强免疫在实际工作中的重要性

应用口蹄疫液相阻断ELISA方法对新疆精河县规模场母牛血清进行了检测,加强免疫组的免疫保护率高于一次免疫组的免疫保护率,加强免疫在防疫工作中消除隐患,减少了防疫漏洞。     

采食水平对东北民猪代谢热量的影响

以哈白猪为对照，研究２０℃条件下，低（Ｌ，７５０ｇ／ｄ），中（Ｍ１０５０ｇ／ｄ）高（Ｈ，１３００ｇ／ｄ）三种采食水平（饲料ＭＥ含量为１２．３７ＫＪ／ｋｇＣＰ：１６％）对体重２０～２５ｋｇ的民猪产热量的影响，研究结果表明，随采食水平提高，产热量有逐渐增加的趋势，但产不显著（Ｐ〉０．０５），三种采食水平下，民猪和哈白猪的产热量分别为３３．５，３４．５，３５．９和３１．９，３３．８，３４．９ＫＪ／ｋｇ＾     

基于高密度SNP芯片技术对中国西门塔尔牛SCD1基因与肉质性状的相关性分析

试验借助高密度SNP芯片技术在整个基因组范围内对223头中国西门塔尔牛群体进行基因分型,在SCD1基因上发现7个SNPs位点,对各SNP位点与西门塔尔牛肉质性状进行相关性分析。结果表明,SCD1基因的7个SNPs位点均与大理石花纹等级显著相关(P<0.05或P<0.01);A10050C、A13655G、C14790T和A15565G4个位点对剪切力影响显著(P<0.05);位点A13655G对肌内脂肪含量影响显著(P<0.05);位点A10050C、C14790T、A15565G不同基因型个体间脂肪颜色差异显著(P<0.05)。单体型分析结果显示,7个SNPs位点共构成6种单体型和14种单体型组合。单体型组合H3H6与高肌内脂肪含量极显著相关(P<0.01);单体型组合H6H6与优质大理石花纹极显著相关(P<0.01);单体型组合H4H6与高剪切力值显著相关(P<0.05);各单体型组合对肉色和脂肪颜色影响不显著。     

猪圆环病毒2型Rep蛋白ELISA抗体检测方法的建立

为建立一种快速检测猪圆环病毒2型(PCV2)抗体的方法,本研究通过基因合成PCV2的Rep编码序列,将其克隆至pET-28a(+)并在E.coli中表达,表达的重组Rep蛋白经Ni2+亲和纯化.采用纯化的目的蛋白作为包被抗原,建立检测PCV2 Rep蛋白抗体的间接ELISA方法.结果表明最佳抗原包被浓度为200 ng/孔,血清和二抗最佳反应时间分别为1h和30 min.该方法具有良好的特异性和敏感性,与韩国金诺公司试剂盒的符合率为81.4％,表明建立的ELISA方法可以用于PCV2的流行病学检测.     

宠物兔肠道寄生虫感染情况调查

为了解宠物兔肠道寄生虫感染情况,采用卢戈氏碘液染色法和饱和蔗糖溶液漂浮法对1个宠物市场和2个宠物兔场的305份兔新鲜粪便样品进行检查。结果检出肠道寄生虫5种,总感染率为71.2%,其中球虫感染率最高,为47.2%,隐孢子虫、贾第虫、圆线虫和栓尾线虫感染率分别为2.0%、3.9%、29.5%和1.0%;不同采样点和不同年龄段宠物兔肠道寄生虫的感染率统计学差异均极显著(P<0.01),寄生虫感染随年龄增长呈递减趋势。数据表明宠物兔肠道寄生虫感染率较高,且存在人兽共患机会性原虫,应加强其寄生虫病的防治。     

Effect of different fibre sources on performance,carcass characteristics and gastrointestinal tract development of growing Greylag geese

1. The effects of different fibre sources on the growth performance, carcass characteristics and gastrointestinal tract development were studied in growing Greylag geese (Anser anser).

2. Four experimental diets were formulated with corn (maize) straw silage (CSS), steam-exploded corn (maize) straw, steam-exploded wheat straw, and steam-exploded rice straw as fibre sources. A total of 224 male Greylag geese at 28 d of age were randomly assigned to one of the 4 experimental diets.

3. The birds fed on the CSS diets had higher average daily feed intakes than those fed on the steam-exploded straws. However, the 4 treatments had similar average daily gain, which contributed to significant differences in feed conversion ratios. The different fibre sources had no significant effects on the carcass characteristics.

4. The CSS-fed birds had larger gizzards and lower relative length of the caeca than the other three groups. However, the relative weights and lengths of the other gut segments, the relative weights of major organs and the pH values of the gastrointestinal contents were similar between the 4 treatments.

5. It was concluded that straw fibres with different physico-chemical properties exerted an effect on daily feed intake and gastrointestinal development, especially for the gizzard. The pretreatment of straw had a large effect on utilisation efficiency and animal performance. Steam explosion is a promising straw pretreatment for inclusion in diets for geese.

     

3种不同方法对肉牛胴体性状预测能力的比较研究

本研究为了寻求一种对肉牛胴体性状预测准确性较高的方法,运用DPS数据处理系统和SAS软件比较偏最小二乘回归、GM(1,N)灰色系统和BP神经网络3种常用的预测模型对肉牛胴体性状的预测能力。选择肉牛7个宰前生长性状(体高、体长、胸围、腹围、管围、宰前活体质量、平均日增体质量),对2个重要的胴体性状(胴体质量和净肉质量)进行预测。结果表明:偏最小二乘回归方法在肉牛胴体性状预测方面准确性最高;GM(1,N)灰色系统和BP神经网络预测准确度偏低。本研究还将3种预测结果相结合,取其均值,大大提高了预测的准确性。这一研究将为肉牛生产实践提供一定的科学参考。     

猪精子质量的流式细胞检测分析

本试验将流式细胞术应用于猪精子分析,同时检测精子功能的多个特性,以保证精子具备多种特性且功能完整,顺利完成授精过程。分别采用SYBR-14/PI（Propidium Iodide）、YO-PRO-1/PI、PNA/PI及Mito Tracker/YO-PRO-1 4种染色方法对荣昌猪精子的质膜完整性、细胞凋亡、顶体完整性及线粒体功能进行了检测和分析;以SYBR-14⁺/PI^-表示质膜完整的活精子,YO-PRO-1^-/PI^-表示活精子,PNA^-/PI^-表示未发生顶体反应的活精子,Mito Tracker⁺/YO-PRO-1^-表示线粒体活性高的活精子,以上述检测指标的综合结果判定精子功能的完整性。本试验通过流式细胞术对荣昌猪精子进行质量检测和分析,结果表明,该方法可为临床诊断种猪不孕症提供理论依据,对有效治疗不孕症有重要意义。     

方正智绘Mirage GIS在资源调查上的二次开发

论述了方正智绘Mirage GIS在森林资源调查中的二次开发,详细介绍了方正智绘MirageGIS进行二次开发的对象模型,方法,并在Visual Basic开发环境下进行具体实例的开发应用。     

Cloning,Expression of Clostridium perfringens α-toxin Gene and Preparation of its Antisera

One pair of primers had been designed and synthesized based on the α-toxin gene of Clostridium perfringens.The complete α-toxin gene fragment was amplified by polymerase chain reaction (PCR), and then was cloned into pGEM-T Easy vector to construct pGEM-T-α.Digested with EcoRⅠ and Hind Ⅲ, a fragment of 1125 bp was cloned into the expression plasmid vector pET-28a(+).The recombinant plasmid was transformed into the BL21(DE3)plys and induced by 1.0 mmol/L IPTG at 37 ℃.The expression product was found to be 46.1 ku as expected one identified by SDS-PAGE, and confirmed by Western blotting with Clostridium perfringens type A antisera, indicating similar reactivity with native α-toxin.Recombinant α-toxin protein was simultaneously found in culture supernatant, postsonic supertanant and inclusion bodies, most protein was expressed in inclusion bodies, which indicated recombinant α-toxin protein was expressed in the extracellular, periplasm and cytoplasm.Recombinant α-toxin protein in postsonic supertanant could not make mice die, indicating its non-toxicity.Toxin-antitoxin neutralization test showed that antisera of recombinant α-toxin protein were specific to α-toxin.Upon immunization of rabbit with the recombinant α-toxin protein, antisera with high antibody titer neutralizing 100 MLD toxin per 1 mL were prepared.