双孢蘑菇mtDNA粗提物的酶切分析

发展了一种粗提双孢蘑菇mtDNA的方法，获得As2796亲代及子代系列菌株的mtDNA粗提物，用HaeⅢ、CfoI、MspⅠ、TaqⅠ等内切酶进行酶切，并对酶切结果进行了分析和讨论。     

Long-Range Transport of Sulfur from Northeast Asia to Chengshantu, Shandong Peninsula: Measurement and Simulation

Observations of air pollutants were conducted at Chengshantu in January 1996 to clarify the extent of trans-boundary pollution from the Asian continent. A nested air quality prediction modeling system (NAQPMS), which included parameters on emission, transport, diffusion, deposition and transformation of sulfur oxides, was performed to compile sulfur concentrations over the observation site. The model calculation reproduced the observed variations of sulfur oxides and sulfate well, although the model calculations could not reproduce the extensively low sulfate concentrations. Using nesting improves the ability of the modeling system to capture peak episodes seen in the observations. Calculation of the origins of deposited sulfur in each country with the NAQPMS shows that the long-range transport of sulfur is very serious. For Japan, 49% of sulfur deposition was from other countries during the campaign period.     

鸡腿蘑栽培菌株的SRAP分析

对21个鸡腿蘑栽培菌株的基因组DNA进行SRAP分析,9对引物共获得23条明显的多态性扩增条带,利用23条扩增条带对菌株进行聚类分析,获得亲缘关系树状图.结果显示:不同菌株间存在着丰富的遗传多样性,扩增指纹能有效分辨不同菌株的基因型.在60%的相似值上,21个菌株可以分为5大类,而在100%相似值上,除2个菌株Co.0071和Co.0072未能区分外,其余19个菌株均可单独分开.     

中国野生蘑菇属90个菌株遗传多样性的DNA指纹分析

对90个中国野生蘑菇属菌株(其中44株经同工酶初步鉴定为双孢蘑菇菌株)的总DNA进行SRAP和ISSR分析,获得了18条SRAP和12条ISSR标记条带并进行聚类分析,构建了亲缘关系树状图.结果显示这些菌株大体上可分为野生双孢蘑菇和野生蘑菇属其它菌株两大类群,其中来自川藏高原的41个野生双孢蘑菇按照采集地的不同聚为4个群,地域性差异比较明显;部分来自新疆、西藏的白色野生双孢蘑菇菌株新疆野生、AgX04和AgX042具有独特带型,与其它双孢蘑菇菌株的遗传相似值仅为10%～13%.     

铝阳极氧化膜的界面性能与常温封闭

采用电渗法研究了几种电解质溶液对铝阳极氧化多孔膜界面性能的影响．结果表明，含有Ｆ－，ＳｉＯ２－３，ＰＯ３－４，Ｓ２Ｏ２－３和酒石酸根等的电解质溶液，使多孔膜的等电点ｐＨＩＥＰ由原来的９．２０分别移至３．４０，３．３０，４．３０，３．９０和３．８０，膜的带电性能发生了相应的变化．多孔膜带电性能的变化有利于常温封闭液中的金属离子进入多孔膜内，同时Ｆ－，Ｓ２Ｏ２－３还可通过与膜内Ｈ＋，Ｈ２Ｏ等的相互作用，使膜孔内ｐＨ升高，从而加速金属离子在膜内的水解沉积，促进常温封闭的进行．     

茯苓栽培菌株的RAPD分析

笔者应用RAPD分子标记技术对14个茯苓栽培菌株进行遗传多样性分析,以期探究茯苓栽培菌株间的亲缘关系,为茯苓菌株的鉴别及育种提供DNA依据。研究中应用9个RAPD引物对茯苓栽培菌株进行RAPD分析,筛选出4个RAPD引物,扩增出21条比较明显的条带。利用21条扩增带对14个菌株进行聚类分析,获得14个茯苓栽培菌株亲缘关系树状图。结果显示在53%的相似值时,14个茯苓栽培菌株可分为2大类群,而在93%相似值时可分为13个类群。     

Long-Term Soil Acidification Model (LTSAM) Development and Application for Analyzing Soil Responses to Acidic Deposition

A long-term soil acidification model (LTSAM) which can describe calcareous and non-calcareous soil responses to acidic deposition is developed based on the conservation of alkalinity and Ulrich buffer ranges. The model which considers nine major ions of armospheric deposition, has focused on certain soil processes (weathering of carbonates, silicates, and aluminum (Al) oxides or hydroxides, cation exchange, anion retention, and CO2 solubility). After comparing the model design and simulation results with the SMART, the paper describes several numerical experiments on the sensitivity of calcareous drab soil in Beijing and red earth (nearly dystric cambisol) in Wenzhou of Zhejiang Province in Southern China to acidic deposition scenarios. The modelling results indicate that increase or decrease in atmospheric deposition of base cations and not only changes in deposition of sulfate (S) and H+ must be considered in assessment of critical loads both for red earth and calcareous drab soil.     

印度鹰嘴豆资源遗传多样性分析及优异资源筛选#br#

为了解并有效利用印度鹰嘴豆种质资源农艺性状的多样性,通过遗传多样性分析、聚类分析、主成分分析和综合D值评价等方法对181份印度鹰嘴豆资源的12个农艺性状的多样性水平进行分析,筛选可用于评价鹰嘴豆资源的农艺性状及综合表现良好的种质。结果表明：12个表型性状的变异系数范围为7.47%（播种至开花期天数）～85.26%（秕荚数）,百粒重、分枝数、有效分枝数、实荚数、见花至开花期天数、产量、单株产量和秕荚数的变异系数均大30%,具有很好的改良潜力;Shannon多样性指数范围为1.750 9（单株产量）～2.053 5（单荚粒数）,平均值为1.930 2,单荚粒数、株高、有效枝数和播种至见花期天数的多样性指数均大于2.000 0,12个农艺性状的遗传多样性指数均大于1.700 0,说明各性状的数量指标分布较为均匀;在欧式距离为3.41处,181份印度鹰嘴豆资源被分为9个类群,各类群资源数量不同,第Ⅰ～Ⅷ类群均具有一定的性状优势;筛选到4个可用于综合评价印度鹰嘴豆资源的主要表型性状,分别为实荚数、播种至开花期天数、株高、分枝数;利用综合D值评价筛选到31份综合表现较好的资源,为我国鹰嘴豆种质创新和新品种选育提供理论基础和优异的基础材料、亲本来源。     

外引鹰嘴豆云南适应性评价

评价9份引进抗旱鹰嘴豆材料在云南省不同生态区域适应性,筛选适宜各生态区域种植的鹰嘴豆品种进行推广种植。选取玉溪、曲靖、丽江、保山、文山5个不同生态区域,对鹰嘴豆产量、株高、有效枝、百粒重、单株荚数、单株粒数等6个性状进行评价。结果表明,文山地区各品种表现良好,产量最高。曲靖地区次之,各品种均表现良好,增产比率达100%,产量中等。丽江、保山地区个别品种表现良好,品种间差异显著,玉溪地区产量最低。Y-11-47、Y-11-39丰产性、稳产性及适应性均表现良好,Y-Ebony、Y-11-37产量较高,稳定性及适应性稍差。文山地区是鹰嘴豆的最佳种植区,曲靖次之。丽江、保山地区适宜个别品种推广种植。Y-11-47、Y-11-39适宜广泛推广种植。Y-Ebony适宜在丽江、文山、保山地区及相似生境区种植,Y-11-37适宜在曲靖、保山、文山地区及相似生境地区种植。