Persistent, widespread papilloma formation on the penis of a horse: a novel presentation of equine papillomavirus type 2 infection

A 9-year-old gelding presented with approximately 100 papillomas that covered about 75% of the distal penis. Biopsy was performed, and histology showed evidence of viral cytopathic change and koilocytosis. Polymerase chain reaction using DNA extracted from biopsied tissue amplified equine papillomavirus type 2 (EcPV-2) DNA sequences. Sixteen months later, the horse was re-examined and the appearance of the papillomas was unchanged. Equine papillomavirus type 2 DNA sequences were again amplified from both biopsied tissue and swabs of the penis. Papillomavirus was localized to the lesions by immunohistochemistry and in situ hybridization. An examination 2 years after the initial presentation revealed no detectable change in the appearance of the penis. The large number of papillomas and their failure to regress over an extended period support a clinical classification of papillomatosis. To the authors' knowledge, this is the first report of papillomatosis of the equine penis. This novel clinical manifestation suggests that persistent EcPV-2 infection is possible in horses. As there is evidence that EcPV-2 may promote development of equine penile squamous cell carcinoma, understanding the natural history of EcPV-2 infections may be important in preventing equine penile neoplasia.     

Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories

This document is the consensus of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization of Immunohistochemistry on a set of guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. Immunohistochemistry is a powerful ancillary methodology frequently used in many veterinary laboratories for both diagnostic and research purposes. However, neither standardization nor validation of IHC tests has been completely achieved in veterinary medicine. This document addresses both issues. Topics covered include antibody selection, fixation, antigen retrieval, antibody incubation, antibody dilutions, tissue and reagent controls, buffers, and detection systems. The validation of an IHC test is addressed for both infectious diseases and neoplastic processes. In addition, storage and handling of IHC reagents, interpretation, quality control and assurance, and troubleshooting are also discussed. Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories.     

Estimation of stem volume using laser scanning-based canopy height metrics

The aim of this study was to test different stem volume predictorsthat are capable of utilizing laser scanning-based canopy heightmetrics as independent variables. The three laser scanning-basedmethods compared were (1) a direct prediction model for thestem volume at plot level, (2) a volume prediction system basedon the modelled percentiles of the basal area diameter distribution,and (3) a parameter prediction method used to determinate Weibull-basedbasal area diameter distributions for the plot-level stem volumeprediction. The predicted volumes were also compared with field-measuredvolumes obtained with the Finnish conventional inventory bycompartments. The best results were obtained with the firstmethod, i.e. the model that predicts plot-level stem volumesdirectly, which is logical. Furthermore, the simulated reductionof point density of laser data had no effect on the accuracyof stem volume predictions. The percentile-based modelling ofdiameter distributions was applied, in particular, to the determinationof non-homogenous stand structure; using this method, it iseven possible to fit multimodal distributions. In terms of theaccuracy of the predicted plot-level stem volumes, the volumeprediction method based on modelled percentiles of basal areadiameter distributions was the second best, whereas the volumeprediction method based on the parameter prediction of the Weibull-basedbasal area diameter distributions resulted in slightly worseresults. However, the accuracies of the three laser-based volumeprediction methods tested were superior to the published resultsof spectral value-based remote sensing studies implemented usingdata collected from Finland. Furthermore, the accuracy of plot-levelstem volume estimates calculated from field assessments wasconsiderably weaker than the accuracy of the three volume predictionmethods that utilized measures obtained with laser scanning.     

Production strategies in the Swedish softwood sawmilling industry

The strategic production decisions to improve profits for sawmills can be described in three main dimensions. More value-added production increases unit revenues, larger volumes reduce fixed costs per produced unit, and improved productivity reduces unit costs. Using data about the Swedish sawmills and cluster analysis, six groups of sawmills were defined with these strategy dimensions. Characteristics of each strategy are presented concerning ownership, location, technology, and economic indicators. The results indicate that different groups of Swedish sawmills employ different production strategies, which are reflected both in the dimensions, value-added shares, size, productivity, and by other technical and economic characteristics of the sawmills. The relationships between production strategy and profitability is also analysed and discussed.     

Eastern equine encephalitis in 9 South American camelids

BACKGROUND: Eastern equine encephalitis (EEE) virus is a mosquito-borne togavirus (alphavirus) that causes severe (often fatal) encephalitis in many mammalian species, but it has not been reported previously in South American camelids. Hypothesis: South American camelids can become naturally infected with EEE virus and show encephalitic signs similar to those observed in other affected species. ANIMALS: Nine cases (8 alpacas and 1 llama, aged 3.5 weeks to 12 years) were identified; 4 of 9 were 510 weeks old. All cases were from the East Coast of the United States and presented in late summer and fall. METHODS: A retrospective study was performed to include confirmed cases of EEE in camelids in North America before 2006. RESULTS: Eight of nine (89%) camelids died or were euthanized in extremis, with the mean time to death of 2 days. Clinical signs were consistent with encephalitis and included fever, lethargy, ataxia, seizures, recumbency, torticollis, opisthotonus, and vestibular signs. No consistent hematologic abnormalities were identified, and cerebrospinal fluid contained an increased protein concentration in the single camelid analyzed. No successful therapy was identified. EEE was confirmed by alphavirus detection by using immunohistochemistry (IHC) and polymerase chain reaction (PCR) in the central nervous system (CNS) and by serology. Findings included polioencephalitis with lymphocytic perivascular cuffing; neutrophil infiltration; gliosis; neuron satellitosis; necrosis; and edema, with intracytoplasmic alphavirus within neurons and glial cells. No virus was detected in extraneural tissues. CONCLUSIONS AND CLINICAL IMPORTANCE: In endemic areas, EEE should be considered a differential diagnosis for young and adult camelids with CNS disease. Brain histopathology with indirect IHC or PCR is diagnostic.     

Determination of the spatial distribution of trees from digital aerial photographs

This study examined the possibilities of using computerized digital aerial photograph interpretation in determining the spatial distribution of trees. The material of the study included eight mapped stands in the municipality of Hyytiälä (61°50′N and 24°18′E), in southern Finland. The aerial photographs used were taken in June 1995 at a scale of 1:5000. Two approaches for determining the spatial pattern of trees were used. Firstly, in the point-process based approach used in this study, the individual trees in the digital aerial photograph were segmented by a robust segmentation method, based on recognition of the pattern of tree crowns with sub-pixel accuracy. Secondly, the crown coverage was determined by region growing segmentation combined with active surface representation. The significance of the differences in the means of image coverage pattern indices between the various spatial distribution categories was tested with one-way variance analysis. Because the process misclassified clustered spatial patterns as regular patterns, and regular patterns as random patterns, the usability of digital aerial photographs seems to be limited for the point-process based determination of the spatial pattern of trees if the scale is 1:5000 or less. When image coverage pattern indices were applied, the differences in the means of the spatial distribution categories proved not to be clearly statistically significant due to the great variation within classes. However, interpretation of crown coverage could have applications in practical forestry due to the low resolution requirements for the images used.     

Dehydrated mink manure as a protein source for growing pigs

The use of dehydrated mink manure as a protein source for growing pigs was studied by substituting 7.5 and 15 % (w/w) of the complete feed with manure and a corresponding amount of barley. The manure used had a poor feed value as compared to the values obtained earlier in digestibility tests in minks. The crude protein content was 21.3 %. The addition of mink manure to the feed did not adversely affect the feed consumption, nor were any effects on the health of the pigs observed. The differences in daily gain and feed efficiency recorded between the experimental groups were not statistically significant. The number of experimental animals was small, and a large variation occurred within the groups. The addition of mink manure to the diet resulted in lower weight gain, apparently due to a lower energy supply. The groups given mink manure received less feed units than the controls. No harmful effects of the manure feeding on carcase quality was observed and organoleptic tests revealed no changes in the smell or taste of the meat. No changes were found in the digestive tracts, livers or kidneys of the pigs.     

Vertebral malformation, syringomyelia, and ventricular septal defect in a dromedary camel (Camelius dromedarius).

An occipitoatlantoaxial malformation and ventricular septal defect (VSD) were diagnosed in a 36-hour-old female camel. Physical examination revealed a firm protrusion of the dorsal aspect of the atlas and axis, tilting of the head to the left, and a grade V/VI systolic murmur. Neurological examination revealed proprioceptive deficits and ataxia of all 4 limbs. Radiographic examination and necropsy demonstrated malformation, fusion of the atlas to the occiput and hypoplasia of the dens of the axis, and subluxation of the atlantoaxial joint. Dorsoventral laxity of the atlantoaxial joint was also present, with compression of the cervical spinal cord. A 1.5-cm-diameter VSD was observed also. Histopathologic examination of the cervical spinal cord revealed a cavity extending from the level of the first to fourth cervical segment, dorsal to the central canal, 5 cm long and 1-2 mm in diameter. The cells around the cavity were positive for glial fibrillary acidic protein and sporadically positive for vimentin. This cavitary structure was consistent with syringomyelia, which was lined by glial cells, surrounded by edematous white matter with Wallerian-like degeneration and with neuronal necrosis in the adjacent dorsal horns.