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301.
Igor Renan Honorato Gatto Daniel Correia Lima Linhares Henrique Meiroz de Souza Almeida Luis Antônio Mathias Andrea Souza Ramos de Medeiros Zvonimir Poljak Samir Issa Samara Luis Guilherme de Oliveira 《Tropical animal health and production》2018,50(4):773-778
Bovine viral diarrhea virus (BVDV) infects ruminants as primary hosts. However, other animals like pigs are susceptible. This study was conducted to investigate seroprevalence and risk factors associated with the detection of BVDV antibodies in pig herds. A total of 1.705 serum samples of 33 finisher herds, from seven Brazilian states, were collected in slaughterhouses. The samples were tested by virus neutralization (VN) test. In total, 5.35% (91/1.705) were positive and 64% (21/33) of the herds had positive animals. A significant association with “trucks are not cleaned and disinfected” and “visitors do not respect 72-h interval between visits to farms” (P?<?0.05) was found in association with detection of BVDV-2 antibodies. This study suggests that important biosecurity gaps are present in Brazilian pig farms, as the presence of BVDV antibodies in pigs suggests (direct or indirect) contact with population(s) of ruminant species. Closing biosecurity gaps prevents spread of BVDV and other pathogens such as foot-and-mouth disease virus (FMDV) between pig and ruminant farms. This data should be taken in account by CSF surveillance programs, once cross-reaction in serologic tests between classical swine fever virus (CSFV) and BVDV antibodies has been shown to occur. 相似文献
302.
Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination 总被引:6,自引:0,他引:6
Wienhold D Armengol E Marquardt A Marquardt C Voigt H Büttner M Saalmüller A Pfaff E 《Veterinary research》2005,36(4):571-587
The aim of this study was to determine the immunomodulatory effects of IL-12, IL-18 and CD154 (CD40 ligand, CD40L) in DNA-vaccination against the classical swine fever virus. Four recombinant plasmids were constructed including the CSFV coding region for the glycoprotein gp55/E2 alone or together with porcine IL-12, IL-18 or CD154 genes. Five groups of four pigs each were immunized intramuscularly (i.m.) three times with the respective constructs. The control group was inoculated with empty plasmid DNA. Eighteen days after the final immunization, the pigs were challenged with a lethal dose of CSFV strain Eystrup and monitored for a further 16 days. This study showed that co-delivery of IL-18 and CD154 induced an earlier appearance of serum antibodies, reduced B-cell deficiency after infection and protected pigs against a lethal CSFV infection. In contrast, co-delivery of IL-12 led to a reduced titer of neutralizing antibodies and protection against a lethal CSFV challenge in comparison to the other pigs and to pigs that were immunized with a gp55/E2 plasmid alone. 相似文献
303.
Gunther Antonissen Siska Croubels Frank Pasmans Richard Ducatelle Venessa Eeckhaut Mathias Devreese Marc Verlinden Freddy Haesebrouck Mia Eeckhout Sarah De Saeger Birgit Antlinger Barbara Novak An Martel Filip Van Immerseel 《Veterinary research》2015,46(1)
Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-015-0234-8) contains supplementary material, which is available to authorized users. 相似文献304.
Henrique Meiroz de Souza Almeida Gabriel Yuri Storino Daniele Araújo Pereira Igor Renan Honorato Gatto Luis Antonio Mathias Hélio José Montassier Luís Guilherme de Oliveira 《Tropical animal health and production》2017,49(1):25-30
Swine influenza (SI) is a seasonal infectious disease highly important to the world pig industry. Loss of daily weight gain, increased costs for the prevention and treatment of secondary infections are the main economic losses associated with the presence of this disease. However, some epidemiological features of SI remain quite unclear. This study focused on assessing the prevalence of swine influenza virus (SIV) infection in intensive and extensive pig herds and associating risk factors. A set of 601 blood samples of five intensive farrow-to-finish farms and 361 blood samples from 56 extensive farms were analyzed using an indirect ELISA kit CIVTEST SUIS INFLUENZA®, Hipra (Amer, Spain), in order to detect anti-SIV antibodies. In total, 24.13 % of samples from intensive herds were positive, while no positive samples were detected in extensive rearing herds. Sow and weaning piglets had the highest prevalence values. In the intensive rearing system, occurrence of reproductive disorders and exposure to recently introduced animals were positively associated with the disease occurrence in swine herds. The findings highlight the importance of sows in the epidemiology of the disease and bring information about risk factors involved in the occurrence of swine influenza in intensive herds. 相似文献
305.
在对青海湖高寒湿地进行群落调查的基础上,研究了在群落梯度上高寒湿地的生物多样性与生产力的关系,并探讨了在同一区域内环境的整体性对高寒湿地的生物多样性与生产力关系的影响。运用SPSS软件进行曲线拟合,研究了青海湖内陆高寒湿地物种多样性和地上生物量的关系。研究结果表明,(1)无论是在群落尺度上还是在区域尺度上物种多样性指数与地上生物量呈显著的负相关。(2)在研究区内区域因素对物种多样性和生产力的影响较弱,但如果保持区域环境的整体性则可以有效地提高物种多样性和生产力的相关程度。 相似文献