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21.
Rosalina M. LAPITAN Arnel N. DEL BARRIO Osamu KATSUBE Tomomi BAN-TOKUDA Edgar A. ORDEN Alberto Y. ROBLES Libertado C. CRUZ Yukio KANAI Tsutomu FUJIHARA 《Animal Science Journal》2008,79(2):210-217
The objective of this study was to compare the carcass and meat quality in crossbred cattle and crossbred water buffalo at the same younger age and fed with high roughage in the Philippines. Ten crossbred cattle and 10 crossbred water buffalo, with an average age of 22 months (18–24 months) were used in this experiment. The animals were fed a similar diet, which consisted of 85% Napier or Para grass and 15% concentrate mixture on a dry matter basis, for 180 days before slaughter. Slaughter weight of the crossbred water buffalo was higher (P < 0.05) than that of the cattle, although the dressing percentage was significantly (P < 0.01) lower for the former than for the latter. The estimated lean yield was higher (P < 0.05) in crossbred cattle than crossbred water buffalo. The muscle fiber diameter of loin in buffalo meat was smaller (P < 0.05) than that in cattle. In regard to carcass and meat quality, the crossbred water buffalo was comparable or slightly superior to the crossbred cattle in tenderness, sensory score of color (P < 0.01) and flavor (P < 0.05). These results clearly indicate that at a younger age and even under high roughage‐based fattening rations, crossbred water buffalo are able to produce good quality meat, which will be by no means inferior to that of crossbred cattle in the Philippines. 相似文献
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23.
Ryoko Hirata Satoshi Ito Masatake G. Araki Yasushi Mitsuda Masahiro Takagi 《Journal of Forest Research》2014,19(6):514-522
The effects of late weeding treatment on the growth of young hinoki (Chamaecyparis obtusa) during the sixth growing season after planting were examined. Furthermore, the contribution toward hinoki growth recovery of crown productivity (NAR) and biomass allocation to crown (CAR) were determined. In the late weeding plot, no decline in height growth was observed subsequent to weeding, and growth in diameter at breast height (DBH) and crown projection area (CPA) began to recover subsequent to weeding; however, DBH and CPA were reduced and experienced a 1- to 1.5-year growth delay compared to values associated with the weeding plot at the end of the second year subsequent to weeding. Relative growth rate (RGR) and NAR in the late weeding plot recovered and possessed similar values to those of the weeding plot in the second year subsequent to weeding. CAR values of the late weeding plot were similar to those of the weeding plot both before and after weeding. These results suggested that the recovery of NAR rather than that of CAR was primarily responsible for the recovery of RGR. The present study demonstrated that hinoki were able to quickly acclimate to an environment dramatically altered by late weeding and recover growth rate within a short period of time. The present study also showed that delays in crown expansion associated with late weeding may have impeded subsequent matter production. Therefore, it was concluded that late weeding treatments should be employed only when the associated delays in growth are taken into account. 相似文献
24.
Nishi K Ishiuchi M Morimune K Ohkawa H 《Journal of agricultural and food chemistry》2005,53(13):5096-5104
A monoclonal antibody (mab) selective for the thiomethyl-s-triazine herbicide simetryn was obtained and characterized in enzyme-linked immunosorbent assay (ELISA). An IC(50) value for simetryn was 8.5 ng/mL, and the detection range extended from 1.1 to 70 ng/mL in ELISA. The cDNAs encoding variable heavy chain (VH) and variable light chain (VL) of the mab were cloned to produce various recombinant antibodies. Single-chain variable fragment (scFv) antibodies derived from the mab were characterized in ELISA and showed similar reactivities and specificities to the parent mab. A urea denaturation test revealed that the scFv antibodies bound to simetryn were more stable than those in the absence of antigen. A sandwich ELISA based on VH and VL fragments of the mab was successfully developed and showed similar sensitivity to those based on the mab and scFv antibodies in ELISA. In the recovery experiments using spiked environmental samples, the results obtained in ELISA based on the mab were favorably correlated with those by HPLC. 相似文献
25.
Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays) 总被引:11,自引:0,他引:11
Matsuoka T Kuribara H Takubo K Akiyama H Miura H Goda Y Kusakabe Y Isshiki K Toyoda M Hino A 《Journal of agricultural and food chemistry》2002,50(7):2100-2109
Polymerase Chain Reaction (PCR) techniques are increasingly used for the detection of genetically modified (GM) crops in foods. In this paper, recombinant DNAs introduced into the seven lines of GM maize, such as Event 176, Bt11, T25, MON810, GA21, DLL25, and MON802, are sequenced. On the basis of the obtained sequence, 14 primer pairs for the detection of the segments, such as promoter, terminator regions, and construct genes, were designed. To confirm the specificities of the designed primer pairs, PCR was performed on genomic DNAs extracted from GM and non-GM maize, GM and non-GM soy, and other cereal crops. Because the presence of the corresponding DNA segments was specifically detected in GM crops by the designed primer pairs, it was concluded that this method is useful for fast and easy screening of GM crops including unauthorized ones. 相似文献
26.
Sakurako NEO Iyo TAKEMURA-UCHIYAMA Jumpei UCHIYAMA Hironobu MURAKAMI Ayaka SHIMA Hideki KAYANUMA Taiki YOKOYAMA Satoshi TAKAGI Eiichi KANAI Masaharu HISASUE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):1019
Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver- or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver- and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs. 相似文献
27.
Renato S. A. VEGA Arnel N. DEL BARRIO Percival. P. SANGEL Osamu KATSUBE Jose. C. CANARIA Jose V. HERRERA Rosalina M. LAPITAN Edgar A. ORDEN Tsutomu FUJIHARA Yukio KANAI 《Animal Science Journal》2010,81(5):574-579
The purpose of this study is to compare feeding and other behavior and nutrient digestibility of tropical grade Brahman (body weight (BW) = 231 kg ± 12.4; n = 3) and crossbred water buffalo (BW = 300 kg ± 13.9; n = 3). This experiment on digestibility and measures of muscles of mastication utilized one‐way, and animal behavior two‐way, analysis of variance, respectively. Two video camcorders were installed in each pair of buffalo and Brahman for 24 h period programmed on the 107th, 109th and 111th days of the digestion trials. Frequency and duration of feeding, meal intake, rumination, bolus, chews, drinking, defecating, standing and lying were recorded daily. Muscle diameter of Digastricus, Masseter and Pterygoid and different regions of the tongue were sampled and measured under light microscope using a standard micrometer. Buffalo obtained significantly higher intake of dry matter, roughage, crude protein, total digestible nutrient and metabolized energy than Brahman. This was supported by longer meal duration (P ≤ 0.05), and shorter meal breaks (P ≤ 0.05) of buffalo than Brahman. The diameter of the muscles for mastication was bigger (P ≤ 0.05) in buffalo than in Brahman, which is indicative of stronger chewing ability. Briefly, lesser and slower chewing action; higher intake of roughage and crude protein; and longer resting behavior of crossbred water buffalo than Brahman are all indicative of better digestive and metabolic performance of the buffalo under high roughage feeding conditions. 相似文献
28.
Shinya ISHIHARA Rommel J. HERRELA Daichi IJIRI Hisashi MATSUBAYASHI Miho HIRABAYASHI Arnel N. DEL BARRIO Rodel M. BOYLES Medardo M. EDUARTE Ronilo L. SALAC Libertado C. CRUZ Yukio KANAI 《Animal Science Journal》2010,81(6):635-641
Fecal DNA analysis is a useful tool for the investigation of endangered species. Tamaraw (Bubalus mindorensis) is endemic to the Philippine island of Mindoro but knowledge of its genetic and ecological information is limited. In this study, we developed a species identification method for tamaraw by fecal DNA analysis. Eighteen feces presumed to be from tamaraw were collected in Mount Iglit‐Baco National Park and species‐known feces from domestic buffaloes and cattle were obtained from a farm. Additionally, one species‐unknown fecal sample was obtained in Mount Aruyan Preserve, where the sighting of tamaraw has not been reported in recent years. Based on DNA sequence data previously reported, the genus Bubalus‐ and tamaraw‐specific primers for PCR of cytochrome b gene were newly designed. The Bubalus‐specific primer yielded a 976 bp fragment of cytochrome b for all fecal samples from tamaraw and domestic buffaloes, but not for cattle, whereas the tamaraw‐specific primer yielded a 582 bp fragment for all tamaraw fecal samples and for one of the four domestic buffalo samples. PCR‐RFLP (restriction fragment length polymorphism) analysis of the 976 bp PCR fragment with AvrII or BsaXI provided distinct differences between tamaraw and domestic buffalo. PCR‐RFLP analysis also showed that the species‐unknown sample obtained in Mount Aruyan Preserve, originates from tamaraw. 相似文献
29.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):487-491
Case 1 involved a 4-month-old intact male Somali cat in which peripheral pulmonary artery
stenosis (PPS) was recognized after a cardiac murmur remained following patent ductus
arteriosus ligation. Case 2, which involved a 1-year-old neutered male Norwegian Forest
cat, and Case 3, which involved a 6-month-old intact female American Curl cat, were
referred, because of cardiac murmurs. Grades III to IV/VI systolic heart murmurs were
auscultated at the left heart base in all 3 cats. All cases showed bilateral pulmonary
artery stenosis, although there were no associated clinical signs. In Cases 1 and 2, the
pressure gradient through the stenosis decreased after treatment with atenolol. 相似文献
30.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):331-336
Infective endocarditis (IE) in dogs with cardiac shunts has not been reported previously.
However, we encountered a dog with concurrent patent ductus arteriosus (PDA) and IE. The
dog was a 1-year-old, 13.9-kg female Border collie and presented with anorexia, weight
loss, pyrexia (40.4°C) and lameness. A continuous murmur with maximal intensity over the
left heart base (Levine 5/6) was detected on auscultation. Echocardiography revealed a PDA
and severe aortic stenosis (AS) caused by aortic-valve vegetative lesions.
Corynebacterium spp. and Bacillus subtilis were
isolated from blood cultures. The dog responded to aggressive antibiotic therapy, and the
PDA was subsequently surgically corrected. After a series of treatments, the dog showed
long-term improvement in clinical status. 相似文献