Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro

This experiment was designed to investigate the effects of different concentrations of cellobiose (CB) or a twin strain of Saccharomyces cerevisiae live cells (YST) (20, 40 and 60 mg/60 mL), and CB + YST (60 + 20, 60 + 40, 60 + 60 mg/60 mL) on mixed ruminal microorganism fermentation in vitro. Ruminal fluid was collected from a cow, mixed with phosphate buffer (1:2) and incubated (60 mL) anaerobically at 38°C for 24 h with or without supplement plus 400 mg (dry matter [DM] basis) substrate (hay plus concentrate, 1.5:1). The medium pH numerically decreased with CB and CB + YST, but was unchanged with YST. The total volatile fatty acid and proportion of propionate increased (P < 0.05) in all cases. The proportion of acetate decreased (P < 0.05) with CB and CB + YST, but increased (P < 0.05) with YST and that of butyrate increased (P < 0.05) with CB and CB + YST, but decreased (P < 0.05) with YST. Ammonia‐N decreased (P < 0.05) with CB and CB + YST, but was unchanged with YST. The number of protozoa was unchanged, and that of cellulolytic bacteria increased (P < 0.05) in all cases. Total gas production increased (P < 0.05) in all cases. Methane decreased, hydrogen was unchanged by YST and both gases were unchanged by CB and CB + YST. The in vitro disappearance of DM and neutral detergent fiber increased (P < 0.05) by 11.2% and 8.9%, 9% and 8.5%, and 12.1% and 10.2% in the case of CB, YST and CB + YST, respectively. Therefore, the dietary supplementation of CB and/or YST may improve ruminal fermentation and digestibility.     

Outdoor exposure tests of structural laminated veneer lumber (II): evaluation of the strength properties after nine years

To investigate the durability of structural laminated veneer lumber (LVL), outdoor exposure tests have been conducted since 1990 at a field-testing site at the Forestry and Forest Products Research Institute. This article is the second interim report on the results after 9 years of exposure. Seven kinds of structural LVL with no preservative treatment were subjected to the tests. Almost all the exposed specimens were decayed by a kind of brown rot fungi (Pseudomerulius aureus (Fr.) Julich). The degree of decay varied with wood species; grand fir and western hemlock LVL in particular showed weak resistance against the decay. All the specimens were stored for more than 1 year in a testing room conditioned at 20°C and 65% relative humidity. We then measured the ultrasonic velocity of the specimens by the Pundit method, penetration depth by the Pilodyn method, and bending strength by a conventional bending test. Correlation between nondestructive measurement factors and the density was strong even on LVL with many adhesive layers. The nondestructive testing method was found to be applicable to LVL as well as solid lumber. After the nondestructive measurements, each LVL was cut into three types of specimen (top: T, middle: M, and bottom: B) for the bending tests. The bending strength varied with the type of specimens. Correlation between modulus of elasticity and modulus of rupture was strong even in the decayed specimens.     

Comparison of results from the semiautomated serum bone alkaline phosphatase isoenzyme assay with the periosteal alkaline phosphatase assay for use in rat models

BACKGROUND: Assessment of bone formation activity is an important component of pharmacologic efficacy and toxicity evaluations for compounds in development for osteoporosis therapies. Antemortem biomarkers of bone formation and remodeling in rodents are uncommon. While the periosteal alkaline phosphatase (ALP) assay is a postmortem and laborious means of testing bone-building activity, the semiautomated ALP isoenzyme assay is an antemortem assay that is performed on an automated chemistry analyzer after 2 simple dilutions of the initial serum sample and a short incubation. OBJECTIVES: The goal of our investigation was to determine if the serum bone ALP (BALP) data obtained from the semiautomated ALP isoenzyme assay had a similar pattern of response when compared with the periosteal ALP (PALP) assay for use in pharmacologic screening in rats. METHODS: Serum and bone tissue samples were obtained from orchidectomized Wistar rats, a model of clinically induced osteoporosis. Subsequent bone formation was initiated via treatment with one of several compounds. In study 1, orchidectomized male rats were given either vehicle, dihydrotestosterone or a testosterone derivative subcutaneously every 4 days for 28 days. In study 2, orchidectomized male rats were given either vehicle or compounds A, B, or C by oral gavage daily for 15 days. Blood and tibias were collected at necropsy. Serum was analyzed for BALP activity using a semiautomated ALP assay. Tibias from the same rats were analyzed for PALP activity. RESULTS: Serum BALP activity paralleled PALP activity within each group when compared with the controls. CONCLUSION: Our data indicate that the semiautomated serum BALP isoenzyme assay may be used as a biomarker of bone-building potential in rat models of osteoporosis. This assay affords many advantages to investigators of musculoskeletal diseases, including the potential to measure multiple data points in a single study.     

Effect of long-term administration of a prostacyclin analogue (beraprost sodium) on myocardial fibrosis in Dahl rats

Beraprost sodium (BPS) is an orally active prostacyclin analogue. The effects of BPS on the heart, including coronary circulation improvement, myocardial and vascular protection and anti-fibrosis effect on myocardium interstitium, have previously been demonstrated. However, the effects of BPS on hemodynamics, cardiac function and myocardial contractility in patients in the hypertrophic phase have not been clarified. Therefore, in the present study, the effects of BPS under long-term administration were investigated using the hypertension model of salt-sensitive Dahl rats. Six-week-old Dahl rats were divided into three groups, an 8% high salt diet group treated with BPS (BPS group), an untreated 8% high salt diet group (HHF group) and an untreated 0.3% low salt diet group (Control group), and observations were conducted until 17 weeks of age. In the BPS and HHF groups, the survival rates after 11 weeks of high salt diet intake were 87.5% and 47.1%, respectively (p<0.05). At 17 weeks of age, the atrial systolic peak velocity/early diastolic peak velocity and heart weight index of the BPS group decreased significantly compared with the HHF group (p<0.05). The HHF group exhibited significantly more severe myocardial fibrosis mainly in the endocardial layer of the left and right ventricles compared with the BPS and Control groups (p<0.05). In the present study, long-term BPS administration preserved diastolic function and prevented myocardial interstitial fibrosis in the non-compensatory phase. The results of the present study suggest that BPS is effective for treatment of hypertensive cardiac hypertrophy.     

Conjoint analysis of consumer preference for bluefin tuna

Tuna is one of the most important marine products in Japan, but fishery productivity is decreasing, especially for bluefin tuna (Thunnus orientalis, Thunnus thynnus) because of strict capture limits implemented by regional fisheries management organizations (RFMOs). Such capture limitations are necessitated by a shortage of resources. At the same time, full-cycle farming technology of Thunnus orientalis has recently been developed. This technology is set to achieve productivity levels at an industrial scale. In Japan, however, most consumers are believed to have a negative image of farmed products; therefore effective marketing is necessary. This study employs conjoint analysis via an internet questionnaire to clarify consumer preferences and the determine the possibility of marketing through provision of information to consumers regarding bluefin tuna and the environmental background of the problem. The results show that once consumers are provided with enough information about bluefin tuna, particularly in terms of the underlying resources problem, the willingness to pay (WTP) for full-cycle farmed fish is greater compared to conventionally farmed fish. Furthermore, if the product has an “eco-label,” the WTP increases drastically. Therefore, the informational process is important for marketing, and labeling can be an efficient marketing method.     

Application of a bioeconomics model to examine sustainability of fishery resources in the global market: the case of octopus resource in Morocco

A study was conducted to examine the long-term relationship among price of fish, harvest level, and resource condition using a bioeconomics model. Data on the annual amount of production and prices of octopus (Octopus vulgaris) in Morocco from 1970 to 2006 are plotted on a diagram to trace annual changes of price and production. The plotted pattern suggests a long-term movement of the yield-price equilibrium along the line of a theoretical long-term supply function curve of the bioeconomics model. The plotted data also suggests that there are four stages of resource exploitation for octopus in Morocco: (1) the underexploited stage from 1970 to 1987 which is characterized by small catch and low prices, (2) the maximum sustainable yield (MSY) stage from 1988 to 1998 which is characterized by moderate catch and intermediate prices, (3) the overexploited stage from 1999 to 2001 which is characterized by large catch and intermediate prices, and (4) the reduced stock stage after 2002 which is characterized by small catch and high prices. This result is consistent with the independent scientific research information on octopus harvested in Morocco. Our study indicates that an analysis of market information (i.e., amount of production and price) could be an effective tool in identifying approximate status of fishery resources.     

New Rice Mutants（NR676 and NR827）Low in Nitrate Reduction

Mutants edficient in nitrate reductase(NR)were clasfied into two groups,nia types are deficient in apoprotein and cnx types are lack of Mo-Co.This paper reports the characteristics of low NR mutants,which are not edficient in NRactivity but 40%-60%of the NRactivity of the wild type,Nipponbare.Mu-tants NR676and NR827 were selected as seedings showing poor growth with nitrate as sole mitrogen-source from M2 population.They exhibited chlorate reistance.Genetic analysis in the F1and F2indicated that chlo-rate resistance in the mutants was transmitted by a single recessive nuclear gene and that NR676 and NR827 were induced by a mutation at a single locus.In the F2population,all of the seedings showing yellow green leaves were resistant to chlorate and low in NRactivity,Photosynthetic rate and mRNA expression of NR676and NR827 were lower than wild type.These results suggested that low NR activity and chlorate resistance of NR676and NR827 were Caused by a defect in photosynthetic process.     

Measurement of sea bottom surface backscattering strength by quantitative echo sounder

ABSTRACT:   Measurement theory and method of the bottom surface backscattering strength (SS) using a quantitative echo sounder (QES) are discussed and applied in the ocean near Java, Indonesia. The frequencies of the QES were 38, 70, and 120 kHz. The measurements of bottom echoes and sampling of bottom material by a dredge were done simultaneously. Bottom characterization was based on analysis of particle size distribution for bottom samples taken during the survey. The SS value increases with the increase of the mean diameter of the bottom particles. The SS decreased with increasing frequency. The effectiveness of QES for measurement of the SS along with observation of the depth topography were demonstrated.     

Evaluation of nutritional values of selected commercial fish meal sources in turbot (Psetta maxima) diets

This study was conducted to evaluate nutritional values of selected commercial fish meals (FM) with two experiments in diets of turbot. In experiment I, five diets based on two Peruvian anchovy meals (one is a flame‐dried FM: Peru‐1 and the other steam‐dried FM: Peru‐2), a Danish FM (Danish LT), a domestic anchovy LT meal (Domestic LT) and an Alaskan white FM (WFM) as major protein source were formulated to contain 56% protein. The diets were fed to fish with an initial average weight of 119.4 ± 0.1 g for 9 weeks. Growth rate, feed utilization, whole body and visceral compositions, nitrogen, lipid and energy balances did not significantly change in Experiment 1 among the treatments. In Experiment 2, four diets based on three FMs (Domestic LT, Danish LT and Peru‐1) and their mixture (Mix FM) were formulated to contain 50% protein and fed to triplicated groups of fish with an average initial weight of 51.4 ± 0.1 g for 6 weeks. Once again, there were no significant differences in growth rate and feed consumption among the treatments. However, fish on Mix FM diet had significantly better FCR and PER than the others. The results of two experiment indicated that Danish LT and Domestic LT were better in terms of phosphorus (P) retention and losses than two Peruvian FMs and WFM, suggesting that although different sources of FMs were comparable in terms of growth and feed utilization, they could lead to substantial differences in environmental impacts.     

Automatic counting of FISH-labeled microbes by an LED illuminated detecting apparatus

ABSTRACT:   The Bioplorer (BP) (Matsushita Ecology Systems Co. Ltd, Kasugai, Aichi-ken, Japan) is an apparatus that consists of a light-emitting diode and optical and image analysis systems. This instrument has been developed and used for the semi-automatic counting of total microbes, mainly in food, cosmetics and industrial products. In this study, the applicability of BP to the detection and enumeration of eukaryotic and prokaryotic cells, after the fluorescent in situ hybridization (FISH) procedure, was examined. The cells of a yeast ( Candida albicans ) and a bacterium ( Escherichia coli ) were specifically labeled using an oligonucleotide probe with fluorochrome and then counterstained with 4', 6-diamidino-2-phenylindole (DAPI). The numbers of cells after FISH treatment and DAPI staining agreed well, indicating that the BP optical device and image analysis system had enough sensitivity to detect and quantify eukaryotic and prokaryotic cells. Because of its simplicity and reliability, BP can be a new tool for quantification of specifically labeled target microorganisms, both in industrial products and natural samples.