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981.
Stressors such as transportation, weaning and co-mingling increase susceptibility to bacterial pneumonia in cattle and are associated with elevated levels of endogenous glucocorticoids. To determine the effect of glucocorticoids on the proteins expressed in the fluid lining the respiratory tract, bronchoalveolar lavage (BAL) was performed on cattle treated with dexamethasone or saline and proteins were resolved by two-dimensional electrophoresis (2-DE). Significant changes in expression were observed for 9 of the 363 detected spots, and the identities of these proteins were determined by mass spectrometry. Consistent with the initiation of an acute phase response, the expression of alpha-1-acid glycoprotein (orosomucoid) and alpha-1-antitrypsin was increased and alpha-2-HS-glycoprotein (fetuin) was decreased in the BAL fluid of dexamethasone-treated cattle. In addition, dexamethasone induced the expression of two hydrophobic ligand-binding proteins, adipocyte-fatty acid binding protein and odorant binding protein (OBP), as well as the proteins alpha-enolase, cofilin-1 and immunoglobulin J chain. OBP mRNA expression in bronchial biopsies was quantified by real-time RT-PCR and the 6-fold higher levels of expression observed in dexamethasone- versus saline-treated animals correlated with the changes observed in OBP protein level. These findings demonstrate glucocorticoid-dependent changes in the protein composition of the epithelial lining fluid of the respiratory tract, identifying proteins potentially integral to respiratory disease susceptibility. 相似文献
982.
Karim Sorkheh Behrouz Shiran T. M. Gradziel B. K. Epperson Pedro Martínez-Gómez E. Asadi 《Euphytica》2007,156(3):327-344
Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient method for producing DNA fingerprints and
molecular characterization. Our objectives were to: estimate genetic similarities (GS), marker indices, and polymorphic information
contents (PICs) for AFLP markers in almond cultivars; assess the genetic diversity of almond cultivars and wild species, using
GS estimated from AFLP fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression
and cultivar improvement. The genetic diversity of 45 almond cultivars from Iran, Europe, and America, were studied assaying
19 primer combinations. In addition, several agronomic traits were evaluated, including flowering and maturity times, self-incompatibility,
and kernel and fruit properties. Out of the 813 polymerase chain reaction fragments that were scored, 781 (96.23%) were polymorphic.
GS ranged from 0.5 to 0.96, marker indices ranged from 51.37 to 78.79, and PICs ranged from 0.56 to 0.86. Results allowed
the unique molecular identification of all assayed genotypes. However, the correlation between genetic similarity clustering
as based on AFLP and clustering for agronomic traits was low. Cluster analysis based on AFLP data clearly differentiated the
genotypes and wild species according to their origin and pedigree, whereas, cluster analysis based on agronomic data differentiated
according the pomological characterization. Our results showed the great genetic diversity of the almond cultivars and their
interest for almond breeding. 相似文献
983.
Raquel Sánchez-Pérez Encarnación Ortega Henri Duval Pedro Martínez-Gómez Federico Dicenta 《Euphytica》2007,155(3):381-391
The inheritance of 16 important agronomic traits and its relationship were studied for four years in a population of 167 almond
[P. dulcis (Mill.) D.A. Webb] seedlings obtained from a cross between the French selection ‘R1000’ (‘Tardy Nonpareil’ × ‘Tuono’) and
the Spanish cultivar ‘Desmayo Largueta’. For some traits (blooming and leafing date) descendants segregated between the value
of both progenitors, meanwhile for others the mean of the offspring was lower (bloom density, productivity and ripening date)
or higher (in-shell/kernel ratio and double kernels). As expected, kernel bitterness and self-incompatibility behaved as monogenic
traits. Some important correlations between traits were detected. The implications of the transmission and the correlation
of these traits in the breeding programmes are discussed. 相似文献
984.
Anne-marie Wolters Evert Jacobsen Mary O'Connell Guusie Bonnema K. Sree Ramulu Hans de Jong Herman Schoenmakers Jelle Wijbrandi Maarten Koornneef 《Euphytica》1994,79(3):265-277
Protoplast fusion can be used to produce somatic hybrids of species that cannot be obtained by sexual hybridization. The possibility to introgress genes from Solanum species into the cultivated tomato species Lycopersicon esculentum, and to obtain novel cytoplasm-nucleus combinations (cybrids) was considered as an important strategy to extend the genetic variation available for tomato breeding. Somatic hybrids between L. esculentum and other Lycopersicon species, as well as between L. esculentum and Solanum or Nicotiana species, have been produced. Specific mutants, genotypes with antibiotic resistances, and metabolic inhibition by iodoacetate or iodoacetamide and irradiation were used for the selection of hybrids. In addition, the improvement of protoplast culture techniques and the use of the favourable tissue culture traits derived from species such as L. peruvianum, which have been introduced into tomato by classical breeding, allowed the efficient recovery of somatic hybrids. However, the occurrence of somatic incongruity in fusion combinations of L. esculentum and Solanum and even more in L. esculentum and Nicotiana, did not allow the production of true cybrids and/or fertile hybrids, indicating the importance of both cytoplasm-nucleus and nucleus-nucleus interactions in somatic incongruity. Another problem with fusions between distantly related species is the strongly reduced fertility of the hybrids and the very limited homoeologous recombination between chromosomes of the parental species. Partial genome transfer from donor to recipient through microprotoplast (+) protoplast fusion, and the production of monosomic or disomic chromosome addition lines, light overcome some of these problems. In symmetric somatic hybrids between L. esculentum and S. tuberosum the occurrence of limited somatic and meiotic recombination was demonstrated. Fertile progeny plants could be obtained, though at a low frequency, when embryo rescue was performed on a large scale after backcrossing hexaploid somatic tomato (+) potato hybrids with a tetraploid potato genotype. The potential value of genomic in situ hybridization (GISH) and RFLPs for the analysis of the genome/chromosome composition of the hybrids has been demonstrated for intergeneric somatic hybrids between Lycopersicon and Solanum.Abbreviations cpDNA
chloroplast DNA
- mtDNA
mitochondrial DNA 相似文献
985.
Purpose
The aim of the present study was to investigate the effects of visitors’ activities on soil properties in parks of Tel Aviv-Jaffa city, Israel.Materials and methods
The city was divided into three geographical regions: south, central, and north. This division reflects the course of development of the city from south to north and encompasses various socioeconomic levels of residents. In each region, 9–15 parks were randomly chosen and classified into three groups according to their size (in m2): 2?×?103 to 10?×?103, 11?×?103 to 20?×?103, and 21?×?103 to 50?×?103. In each park soil, 0–2 cm of depth layer was sampled in areas under various levels of visitor pressure from July to August 2011, and organic matter, electrical conductivity, and sodium and chlorine contents were determined. Lawns and path and picnic areas represented low and high visitor pressure, respectively. Observations were conducted to characterize the various land uses of the parks and to estimate the numbers of visitors. Also, questionnaires were used at selected parks to assess visitors’ motivations or reasons for visiting the parks.Results and discussion
Socioeconomic indicators, such as the number of residents, the apartment areas, and the percentages of family households, differed among the regions. Soil properties differed between the areas of high and low visitor pressure: they were influenced by type of human activity, size of the park, and number of visitors.Conclusions
Soil properties are affected by variations in the intensity of park use by visitors and by the type of activities enjoyed by these visitors, both of which depend on the socioeconomic status of the park area.986.
Christine Stark Leo M. Condron Alison Stewart Hong J. Di Maureen O’Callaghan 《Biology and Fertility of Soils》2007,43(5):531-540
Because soil biota is influenced by a number of factors, including land use and management techniques, changing management
practices could have significant effects on the soil microbial properties and processes. An experiment was conducted to investigate
differences in soil microbiological properties caused by long- and short-term management practices. Intact monolith lysimeters
(0.2 m2 surface area) were taken from two sites of the same soil type that had been under long-term organic or conventional crop
management and were then subjected to the same 2.5-year crop rotation [winter barley (Hordeum vulgare L.), maize (Zea mais L.), lupin (Lupinus angustifolius L.), and rape (Brassica napus L. ssp. oleifera)] and two fertilizer regimes (following common organic and conventional practices). Soil samples were taken after crop harvest
and analyzed for microbial biomass C and N, microbial activity (fluorescein diacetate hydrolysis, arginine deaminase activity,
and dehydrogenase activity), and total C and N. The incorporation of the green manure stimulated growth and activity of the
microbial communities in soils of both management histories. Soil microbial properties did not show any differences between
organically and conventionally fertilized soils, indicating that crop rotation and plant type had a larger influence on the
microbial biomass and enzyme activities than fertilization. Initial differences in microbial biomass declined, while the effects
of farm management history were still evident in enzyme activities and total C and N. Links between enzyme activities and
microbial biomass C varied depending on treatment, indicating differences in microbial community composition. 相似文献
987.
Elizabeth Rice 《Genetic Resources and Crop Evolution》2007,54(4):701-713
Using survey data from Jala, Mexico, this case-study evaluates in situ maize conservation of the variety ‘Jala’ (Zea mays L.). Though historically ‘Jala’ was the dominant variety grown in the valley of Jala, today less than 20% of farmers grow
it on only 5% of the maize area. Younger growers of the ‘Jala’ variety specialize in it, growing relatively large amounts
for niche markets. Older, diversified farmers grow small areas for household use and to compete in a local contest. Conservation
of the ‘Jala’ variety has been heavily influenced by shifting ideal concepts of maize, as determined by market and consumption
demands and by a contest designed to promote in situ conservation. The current move away from nationalized purchasing may
favor ‘Jala’s’ continued conservation. 相似文献
988.
Background and Objectives
Soil nitrate nitrogen (NO3 −-N) accumulation is related closely to NO3 − leaching, which is an important issue in groundwater pollution, especially in intensive agricultural areas with saline soils where volumes of water are used in irrigation to avoid salt accumulation in the root zone. However, in the saline environment in Hai River Basin, China, the importance of detailed research into NO3 −-N distribution in the root zone has not been adequately recognized. Considering the impacts of eco-environmental system N and crop production, the present study aimed at contributing to an understanding of the effects of N application rate on soil NO3 −-N distribution, NO3 −-N residue, N loss, and maize (Zea mays L.) yield in this region. 相似文献989.
Joshua M. Tebbs Melinda H. McCann 《Journal of Agricultural, Biological & Environmental Statistics》2007,12(4):534-551
Insect-vectored plant diseases impact the agricultural community each year by affecting the economic value, the quantity,
and the quality of crops. Controlling the spread of disease is an important area in risk assessment, and understanding the
dynamics of vector populations helps researchers to develop effective treatments. In this article, we consider an experimental
design commonly used by researchers who study plant disease and examine large-sample, likelihood-based hypothesis tests that
can be used to characterize disease-transmission behavior in a stratified population. Small-sample size and power results
along with design considerations are provided. We illustrate our testing procedures using two real data examples and provide
recommendations for plant-disease researchers in the field. 相似文献
990.
Abrie J. van der Merwe Albertus L. Pretorius 《Journal of Agricultural, Biological & Environmental Statistics》2003,8(3):328-343
This article applies Bayesian nonparametric techniques of analysis to the mixed linear model. The distribution of the random
effects is specific as a nonparametric prior. A Dirichlet process prior is specified on the space of prior distributions.
A modified Dirichlet process is described and applied using a Gibbs sampler. The approach is demonstrated in an investigation
of the changes over time of packed cell volume in two breeds of cattle. 相似文献