Effect of common bean (Phaseolus vulgaris L.) on the community composition of ammonia-oxidizing bacteria in soil previously cultivated with Medicago sativa

The community composition of ammonia-oxidizing bacteria (AOB) was studied during four stages of plant development in soil cultivated with Phaseolus vulgaris in comparison to unplanted soil, using an alfisol previously harboring the legume Medicago sativa. Denaturing gradient gel electrophoresis (DGGE) patterns of 16S rRNA gene and clone libraries of the same gene suggested that bacteria related to Nitrosospira cluster 3 were dominant in both planted and unplanted soil. Bacteria related to Nitrosomonas cluster 8 (Nitrosomonas communis cluster) were found at all times in planted soil, but appeared only randomly in unplanted soil. Analysis of PCR products of the gene encoding the alpha-subunit of ammonia monooxygenase (amoA) by DGGE and clone libraries only detected Nitrosospira cluster 3-like organisms, but failed to detect sequences related to Nitrosomonas. The results suggest that P. vulgaris does not affect the dominant members of AOB communities (Nitrosospira cluster 3), but could have an effect on the prevalence of Nitrosomonas cluster 8 in this type of legume-planted alfisol.     

Methodological alternatives for calculation of enrichment factors used for assessment of topsoil contamination

Purpose

The research is aimed at comparison of different enrichment factors (EFs) calculated for potentially harmful chemical elements (PHEs) basing on topsoil data from Klaip?da with geological differences between two areas, choice of the reference elements (REs), consideration of the expediency and efficiency of normalisation, the influence of PHE and RE background or upper threshold values on the estimated contamination level of 50 schoolyards and the arising problems, i.e. which PHE should be normalised.

Materials and methods

Composite topsoil samples taken in 50 schoolyards and 28 football fields from 0 to 10-cm depth were air-dried, sieved to <1 mm, milled and analysed for total contents of major elements and As, Ba, Cr, Cu, Mn, Mo, Ni, Pb, Sn, U, V and Zn by energy-dispersive X-ray fluorescence and of Ag and Co by optical atomic emission spectrophotometry. Simple EFs in schoolyards were calculated normalising by concentration factor (CF) of Fe, while complex EFs normalising by weighted average of Al, K and Ti concentration factors (WACFs).

Results and discussion

The variability of RE is shown with significant differences between sandy western and loamy eastern areas and lower differences between schoolyards and football fields. The highest effectiveness of normalisation, i.e. the number of sites where WACF > 1 or CF > 1, is when RE background values for schoolyards differentiated for each area are selected. The formulas for the ratio of different EFs demonstrate that (1) for Co, Ba, Mn, Mo, Pb and Cu, the upper threshold-type EFs exceed the background-type EFs; (2) there is the tendency to obtain lower EFs for the greater part of PHE when using differentiated background of PHE and RE. The problems of estimation of the background of some PHE and the expediency of their normalisation are discussed.

Conclusions

The recommendations are (1) to explore the variability of RE in the urban territory and to find out if there are areas with significant differences in RE content, in this case try to estimate the differentiated background values of RE and PHE, because respective EFs are usually lower; (2) for more effective normalisation, to use background values of RE which correspond to the object of assessment; (3) not to use upper threshold-type EFs; (4) to be careful with interpretation of EFs for those PHEs which have significant negative correlation with RE.

     

Assessing uncertainty of semi‐intensive production of whiteleg shrimp (Litopenaeus vannamei) using partial harvesting programs

We used stochastic models for analysis of the uncertainty involved in semi‐intensive production of shrimp in Nayarit state, Mexico, incorporating partial harvests. Analysis of the database showed that increasing the number of harvests was associated with lower stocking densities, the use of larger ponds, longer cultivation periods, larger final weight of shrimp and total production. Equivalence tests showed that the models adequately fitted the primary data. Monte Carlo simulations indicated that improving management by controlling stocking density and the duration of cultivation increased mean production from 981 to 2573 kg/ha (one partial harvest), from 1808 to 3602 kg/ha (two partial harvests) and from 1364 to 3834 kg/ha (three partial harvests), closely approaching the yields reported in the database. When conducting one and three partial harvests, improved management increased production and the certainty in obtaining the crops, as indicated by diminishing values of the coefficient of variation in output probability distributions. When conducting two partial harvests, however, improved management increased yields, but also increased uncertainty because there was a lower control on production parameters. This does not necessarily imply more uncertainty when conducting two harvests, but that at this stage of knowledge, the primary data only allows detecting limited control on production. Results of a preliminary economic evaluation showed that net revenues ranged from USD$ 2361.1–3488.9, the benefit‐cost ratio from 1.47 to 1.62 and that the best and worse results were obtained by conducting two and one partial harvests. We conclude that the models are useful for analysing uncertainty of semi‐intensive shrimp production incorporating partial harvesting.     

An Improved Gravimetric Method to Determine Total Petroleum Hydrocarbons in Contaminated Soils

A gravimetric method to determine heavy fractions of total petroleum hydrocarbons (TPH) in soils is reported. The method was adapted and calibrated by modifying previous standard methods published, incorporating energy and cost savings where possible. Artificially contaminated soils with different organic matter content, and aged in stationary mode for a period of 8 months were used for calibration. Insufficient solvent evaporation was identified as the most prevalent and largest positive interference in the gravimetric detection. To overcome this, while minimizing the need for heating, a combination of three 10-min rotary evaporator steps and 30 min of vacuum in a desiccator were applied, for a total solvent volume of 60 ml. Hexane was chosen as the extraction solvent and a 40–60 min treatment in an ultrasound bath of 260 W was found suitable to extract 80–95% of TPH extracted by the Soxhlet method. Finally, the use of silica gel for cleanup of co-extracted natural organic matter was found unnecessary, because of the low amounts co-extracted for soils with up to 5% organic carbon, and because the chemical nature of the co-extracted organic matter prevents its selective adsorption to silica.     

Effects of biosolarization as methyl bromide alternative for <Emphasis Type="Italic">Meloidogyne incognita</Emphasis> control on quality of soil under pepper

The Adenosine triphosphate (ATP) content, dehydrogenase, phosphatase, urease, and β-glucosidase activity decreased after manure amendment plus solarization (biosolarization), but they recovered after cropping, reaching levels higher than or similar to those before the biosolarization. However, these biochemical parameters decreased and did not recover with time in the methyl bromide (MeBr) treatment. Potential negative soil effects were assayed by measuring, ecotoxicity (Vibrio fisheri luminescence), germination index, electrical conductivity, and heavy metals content. Biosolarization did not negatively affect these parameters, while MeBr application irreversibly decreased the germination index and ecotoxicity. Higher-quality pepper production (extra and first class) were observed with biosolarization but not with MeBr, but no differences were found for pepper yield. Biosolarization gave a Meloidogyne incognita (M. incognita) incidence similar to that of MeBr application.     

Characterization of rhizobia from <Emphasis Type="Italic">Sesbania</Emphasis> species native to seasonally wetland areas in Uruguay

Naturally growing Sesbania species with tolerance to unfavourable habitats are widely distributed in non-cultivated seasonally wetland areas in Uruguay. We investigated the relative abundance, diversity and symbiotic efficiency of Sesbania punicea and S. virgata rhizobia in three ecologically different undisturbed and water-logged sites in Uruguay. Numbers of native-soil rhizobia infective on S. punicea or S. virgata were low, with higher numbers associated with the presence of S. virgata. Plants of S. virgata inoculated with soil suspension showed aerial and nodule biomass greater than that obtained with S. punicea. The rhizobia nodulating Sesbania species in water-logged lands in different regions of Uruguay were diverse differing in growth rates, acid production, growth at 39°C and in LB medium, host range and symbiotic efficiency. Seventeen representative strains clustered into four groups on the basis of phenotypic characteristics, ARDRA and DNA fingerprinting (GTG₅-PCR). Partial sequence of 16S rRNA from eight of these strains classified them into at least two genera with four species: Azorhizobium doebereinerae, Rhizobium sp. related to R. etli and two different Rhizobium sp.-Agrobacterium. Our results confirm the presence of the specie Azorhizobium doebereinerae as microsymbionts of S. virgata in South America. No strain of Rhizobium etli has previously been reported as a microsymbiont of Sesbania, though R. etli like organisms have also been recovered from Dalea purpurea and Desmanthus illinoensis. Significant increases in dry matter production were obtained with S. virgata plants inoculated with selected rhizobial strains under growth chamber conditions.     

Landscape changes influence the reproductive behaviour of a key ‘capital breeder’ snake (Boa constrictor occidentalis) in the Gran Chaco region, Argentina

Many wild reptile species are threatened by habitat loss. However, the way in which changes in landscape patterns influence intraspecific ecological processes is not completely understood. Boa constrictor occidentalis is an endangered species and has a special conservation value since it is endemic of dry forests in the Gran Chaco region. Because the Gran Chaco is largely threatened due to habitat loss it is necessary to know how landscape changes influence this species. Therefore, we evaluated the effects of forest loss and landscape composition on the reproductive life-history parameters. Landscape changes were assessed by analyzing satellite imagery and reproductive parameters were determined by ultrasound images of the reproductive structures. The obtained results indicate that habitat loss may affect body condition, clutch size and testicular volume of the Argentine boa constrictor. We also found that the spatial pattern of vegetation influences the distribution of females and males in the landscape. Matting aggregations are scarce in shrublands. Therefore, our study shows that forest loss could enhance vulnerability to extirpation through constraints placed on reproduction. We encourage resource managers to evaluate sensitive reproductive life-history parameters as well as habitat deterioration to asses the conservation status of the populations of the Argentine boa constrictor. Since the Gran Chaco forest, a key habitat to the species’ reproduction, is largely threatened, strong conservation action is needed to halt and reverse forest loss in this region.     

Posterotestes unelen gen. et sp. n. (Digenea: Apocreadiidae) from Percichthys trucha (Osteichthyes: Percichthyidae) in Patagonia, Argentina

Posterotestes gen. n. (Digenea: Apocreadiidae, Apocrcadiinae) is proposed for specimens with the following features: spiny body, posterior position of gonads, extension of vitelline follicles up to level of intestinal bifurcation and absent at post-testicular space, long oesophagus and extension of caeca up to anterior testis. Posterotestes unelen sp. n. is described from the intestine of the native fish, Percichthys trucha (Cuvier et Valenciennes) (Osteichthyes: Percichthyidae) from Patagonian Andean lakes.     

The effect of the sampling scale on zooplankton community assessment and its implications for the conservation of temporary ponds in south‐west Spain

• 1. The zooplankton (rotifer and microcrustacean) assemblages of temporary ponds in the Doñana National Park (south‐west Spain) have been compared in two surveys of contrasting scales that resulted in the same number of samples: an extensive survey of 36 ponds sampled in May 1998 (or widespread survey) and a survey of nine ponds sampled four times over 2 years (or cumulative survey).
• 2. The total number of microcrustacean and rotifer taxa was larger in the cumulative survey (43 and 41 taxa, respectively) than in the widespread survey (39 and 34, respectively). Crustacean assemblages became less alike throughout the cumulative survey.
• 3. The presence of invertebrates (Coleoptera, Odonata, Heteroptera and crayfish) and aquatic vertebrates (fish and salamanders) was recorded as an estimate of potential predator impact on zooplankton. Several pond features (water depth, conductivity, pH, chlorophyll a concentration, distance to the nearest permanent pond and to the marsh) were also measured in both surveys.
• 4. A combination of these environmental factors was more strongly related to the similarity matrices derived from the zooplankton assemblages of the cumulative survey (Rho=0.7) than to those of the widespread survey (Rho<0.4). The distance of ponds to the marsh was an important factor in explaining this correlation as well as the strongest factor in the ordination of crustacean assemblages following a CCA.
• 5. Predation by exotic fish in long‐hydroperiod ponds where overflow drains to the nearby marsh (fish source) is the mechanism likely to explain the changes in crustacean composition recorded in the cumulative survey.
• 6. The cumulative survey was more suitable for the study of zooplankton diversity as it rendered a higher number of taxa and gave more insight into the mechanisms that explain taxon richness. Thus, conservation strategies in temporary habitats require a scale of observation that includes long temporal changes.

Copyright © 2006 John Wiley & Sons, Ltd.