全文获取类型
收费全文 | 215篇 |
免费 | 14篇 |
专业分类
林业 | 36篇 |
农学 | 7篇 |
基础科学 | 6篇 |
49篇 | |
综合类 | 10篇 |
农作物 | 7篇 |
水产渔业 | 31篇 |
畜牧兽医 | 58篇 |
园艺 | 7篇 |
植物保护 | 18篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 9篇 |
2019年 | 16篇 |
2018年 | 18篇 |
2017年 | 11篇 |
2016年 | 12篇 |
2015年 | 7篇 |
2014年 | 13篇 |
2013年 | 10篇 |
2012年 | 12篇 |
2011年 | 18篇 |
2010年 | 8篇 |
2009年 | 8篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 7篇 |
2005年 | 13篇 |
2004年 | 1篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1985年 | 3篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1962年 | 1篇 |
排序方式: 共有229条查询结果,搜索用时 718 毫秒
31.
Sperm quality in male <Emphasis Type="Italic">Barbus barbus</Emphasis> L. fed different diets during the spawning season 总被引:1,自引:1,他引:0
Sayyed Mohammad Hadi Alavi Martin Pšenička Tomáš Policar Marek Rodina Jitka Hamáčková Pavel Kozák Otomar Linhart 《Fish physiology and biochemistry》2009,35(4):683-693
Sperm quality of Barbus barbus L. was compared among the three following dietary regimes: Group A, fed 100% commercial diet (Karpico™ containing 33% crude
protein and 6% fat), Group B, fed 78% commercial diet and 22% frozen chironomid (Chironomus plumosus) larvae, and Group C, fed 56% commercial diet and 44% frozen chironomid larvae. Concentrations of polyunsaturated fatty acids
(PUFAs) in Group A, B, and C were 39.1, 42.0, and 44.6, respectively, as a percentage of total fatty acids. Sperm morphology,
volume, concentration and motility, total number of spermatozoa, and osmolality of the seminal plasma were compared during
the spawning season. Dietary regime did not influence sperm volume, concentration, or total number of spermatozoa, osmolality
of seminal plasma, or the percentage of motile sperm, but significantly affected sperm morphology (except for anterior and
posterior parts of the midpiece) and sperm velocity (P < 0.05). Groups B and C showed similar sperm characteristics during the spawning season compared to Group A. Almost all parameters
changed either among or within groups during the spawning season, suggesting differences in terms of the optimal time for
sperm collection. The best time for sperm collection was March for Group A, but April for Groups B and C, when the osmolality
of the seminal plasma measured 289 mOsmol kg−1 and sperm motility was maximal. Spermatogenesis, hydration, and cell decomposition were confirmed as the three major parameters
controlling sperm characteristics during the spawning season. The possible correlation between sperm morphology and motility
requires further study. 相似文献
32.
Shah Mujahid Ali Saito Taiju Šindelka Radek Iegorova Viktoriia Rodina Marek Baloch Abdul Rasheed Franěk Roman Tichopád Tomáš Pšenička Martin 《Fisheries Science》2021,87(1):71-83
Fisheries Science - The cleavage pattern of a vertebrate’s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the... 相似文献
33.
Magdalena Gozdowska Marek Ślebioda Ewa Kulczykowska 《Fish physiology and biochemistry》2013,39(4):863-869
In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide’s SPE extraction was 79–85 %. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC–MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue. 相似文献
34.
Viktoriya Dzyuba Borys Dzyuba Jacky Cosson Sergii Boryshpolets Gunes Yamaner Vitaliy Kholodniy Marek Rodina 《Fish physiology and biochemistry》2014,40(6):1731-1739
Oxidative stress is a possible source of spermatozoa function deterioration. Seminal fluid (SF) protects spermatozoa against reactive oxygen species (ROS) attack during development in testes and transit through the reproductive tract. Spermatozoa curvilinear velocity and percent of motile cells as well as changes in thiobarbituric acid-reactive substance (TBARS) content, superoxide dismutase, and catalase activity, and uric acid concentration in SF were evaluated in sterlet sperm collected from testes 24 h after hormone induction of spermiation and from Wolffian ducts at 12, 24, 36, and 60 h after hormone injection (HI). While testicular spermatozoa motility was not initiated in activating medium, Wolffian duct sperm showed low motility at 12 h, significant increase at 24 and 36 h, and decrease at 60 h. Testicular SF was characterized by the highest level of TBARS and activity of studied enzymes compared with SF from Wolffian duct sperm at 24 h post-HI. In fluid from Wolffian duct sperm, a significant increase in TBARS content was shown at 36–60 h post-HI. In contrast to testicular SF, in SF from Wolffian duct sperm, this increase was not counterbalanced by changes in the studied variables of antioxidant system. This may be the source of the observed decrease in spermatozoa motility parameters 60 h post-HI. The results may confirm a dual role of ROS in fish sperm physiology. The data with respect to decrease in sturgeon spermatozoa motility parameters at 60 h post-HI should be taken into account in artificial sturgeon propagation. 相似文献
35.
Ewa Błońska Marek Pająk Stanisław Małek Kazimierz Januszek 《Communications in Soil Science and Plant Analysis》2017,48(6):692-704
The aim of the present research was to study the long-term effect of serpentinite fertilization with additional nitrogen, phosphorous, and potassium fertilizers on some physicochemical properties and the enzyme activity of acidic soils and needle chemistry in stands of Norway spruce (Picea abies (L.) Karst.). Experimental plots were located in spruce stands in the middle forest zone (900–950 m) on two nappes of the Carpathian flysch: Magura and Silesian. Serpentinite was introduced in autumn 2008 on all plots while the other fertilizers in spring 2009. The effect of fertilization was visible mainly in the humus horizon. No significant changes were found in the deeper mineral soil. The acidity and Al toxicity in the surface horizon were ameliorated through serpentinite fertilization. Five years after fertilization, no significant difference in the fertilization treatments was noted in the chemistry of the spruce needles. 相似文献
36.
Five common fungal strains, Cladosporium cladosporioides, Aspergillus clavatus, Penicillium citrinum, Fusarium oxysporum, and Alternaria alternata, were cultivated in presence of iodide and iodate to evaluate their efficiency in iodine biovolatilization and bioaccumulation. Our results suggest that iodide and iodate bioaccumulation by microscopic filamentous fungi is similar although the biological transformation into volatile iodine compounds is driven by various pathways resulting in higher volatilization efficiency of iodate. Thus, the mobilization of iodate by filamentous fungi is superior to iodide mobilization. Our paper is also the first to compare the iodide and iodate volatilization efficiency by microorganisms. Our results highlight the significant role of filamentous fungi in biogeochemistry of iodine, especially in formation of environmentally reactive volatile forms that may contribute to ozone layer destruction. 相似文献
37.
In greenhouse production most floricultural crops are cultivated in soilless substrates with limited concentrations of the beneficial element silicon (Si). Inclusion of supplemental silicon in greenhouse production is reported to affect the quality of several herbaceous plants that accumulate Si in their tissue. The goals of this study were to evaluate the effects of different Si sources and concentrations on the floricultural quality traits, the Si accumulation in plant tissues and the leaf tissue concentrations of other elemental nutrients of greenhouse produced gerbera (Gerbera hybrid L. ‘Acapella’). Potassium silicate (KSiO3) substrate incorporation or weekly substrate drench, sodium silicate (NaSiO3) foliar application, and rice husk ash substrate incorporation were used as Si supplements. Gerbera plants supplemented with several Si source and rate combinations, particularly NaSiO3 foliar sprays, produced thicker flower peduncles, increased flower diameters, increased height, and flowered earlier than non-supplemented controls. Gerbera plants grown in Si-supplemented media all accumulated higher levels of silicon in leaf, peduncle and flower tissues than non-supplemented controls. Leaf concentrations of macronutrients, such as sulfur and potassium, and micronutrients such as, boron, copper, iron, and manganese, were slightly changed among Si-supplemented plants. Foliar sprays of NaSiO3 at a rate of 150 mg Si L−1 caused stem shortening and flower deformation. However, silicon supplements can improve the floricultural traits of greenhouse grown gerberas, but forms and rates of Si must be optimized. 相似文献
38.
Malik I Danek M Marchwińska-Wyrwał E Danek T Wistuba M Krąpiec M 《Water, air, and soil pollution》2012,223(6):3345-3364
Air pollution emissions were not continually monitored in the Upper Silesian Industrial District (USID), southern Poland, and data is only available for the last 20?years. Long-lasting and severe tree ring reductions in pines growing 5-20 km north of the USID area recorded particularly high levels of air pollution emissions in the period 1950-1990. Especially high amounts of reductions and many missing rings were found in the period 1964-1981. At the same time, pines growing 60?km west of the USID do not record deep ring reductions; this proves that the phenomenon is of a regional nature. Increases in infant mortality and lung, bronchial, and tracheal cancer morbidity rates among males were also recorded in the USID during periods of high air pollution. Infant mortality rates increased several years after the tree ring reductions. Therefore, it may be possible to use tree ring reductions as an early indicator of the occurrence of adverse effects on human health. 相似文献
39.
40.
Martina Vlkova Zbynek Polesny Vladimir Verner Jan Banout Marek Dvorak Jaroslav Havlik Bohdan Lojka Petr Ehl Jitka Krausova 《Genetic Resources and Crop Evolution》2011,58(5):629-644
Ethnobotanical survey was conducted in 101 randomly selected home gardens of Phong My commune, central Vietnam, situated in the buffer-zone of Natural Reserve. Data were collected through semi-structured questionnaires and direct observation. Sixty-seven species belonging to 35 families were identified to be used for various purposes. For each species the botanical and vernacular names, plant parts used and main purposes of use are given. The major use categories reported for plant species were food (86%), medicine (32%) and firewood (32%), however, seventy-seven percent of all inventoried plants have multiple uses. The species Artocarpus heterophyllus Lam., Citrus grandis Osbeck, Citrus reticulata Blanco, Ipomoea batatas (L.) Lam. and Musa spp. were identified as the main sources of plant foods consumed within the households. Statistical analyses indicated by Shannon-Wiener and Margalef indices have shown that local species diversity and richness is affected by home garden size. On the contrary, no relationship between diversity and home garden age was found. On the basis of a cluster analysis of plant species diversity, five home garden types were differentiated. In comparison to previously reported studies on tropical home gardens, the diversity in Phong My is lower, probably due to market-oriented strategy. Nevertheless, based on the results achieved, we can conclude that useful plants cultivated in local home gardens provide valuable foods complementing daily diet and subsequently contributing to socioeconomic status of the households. 相似文献