Residual effect after salmon oil supplementation on semen quality and serum levels of testosterone in dogs

The objective of present study was to evaluate the effects of oral supplementation of salmon oil on seminal parameters and testosterone serum levels in dogs, following also the residual effects for 60 days after treatment. Nine healthy male dogs with proven fertility, weighing between 10 and 36 kg, ageing from 2 to 11 years, of different breeds, fed diets supplemented with salmon oil at the manufacturer's recommended dosage. The parameters measured were sperm volume, motility, vigour, normal morphology and concentration, live/dead ratio, membrane viability by means of HOST test and serum testosterone levels. Evaluations occurred at baseline (D0), after 90 days of supplementation (D90) and at the end of the experiment, 60 days after supplementation cessation (D150). Results (mean ± SD) obtained at time D0, D90 and D150 were as follows: motility of 76.66% ± 13.7, 92.77 ± 4.41 and 93.0 ± 7.90 (p = .001); normal spermatozoa of 69.11% ± 24.90, 90.00% ± 5.15 and 80.66 ± 16.04 (p = .05); live/dead (%) from 64.44 ± 22.86 to 85.33 ± 8.41 (p = .001); and spermatozoa (%) with integral membrane in the membrane integrity (HOST) test ranging from 76.44 ± 20.74 to 91.22 ± 4.68 (p = .05). Serum levels of testosterone (ng/ml) increased from 5.50 ± 1.13 to 8.84 ± 1.13 at D90 (p = .003) and decreased after 2 months (D150) to 5.13 ± 1.13. In conclusion, a 90‐day supplementation with salmon oil had a positive influence on semen quality and serum testosterone levels. The supplementation of omegas 3 and 6 at the ratio of 10:1 for 90 days determined an increase in concentration and motility of the sperm, and these effects were maintained for 60 days, with the only exception of testosterone levels.     

Real‐time quantitative PCR‐based detection of Coxiella burnetii in unpasteurized cow's milk sold for human consumption

Coxiella burnetii is a zoonotic pathogen with a worldwide distribution that is responsible for Q fever in humans. It is a highly infectious bacterium that can be transmitted from cattle to humans through the consumption of unpasteurized milk. We report the molecular identification of C. burnetii in raw cow's milk being sold directly for human consumption in Brazil without official inspection or pasteurization. One hundred and twelve samples of raw milk were analysed by real‐time quantitative PCR (qPCR), and C. burnetii was detected in 3.57% (4/112) of the samples at a concentration ranging from 125 to 404 bacteria per millilitre. The identification of this zoonotic pathogen in raw milk sold directly for human consumption is a public health concern since C. burnetii can be transmitted through the oral route. This result indicates that health education and other preventive measures should be officially implemented in Brazil to prevent the spread of infection. To our knowledge, this is the first qPCR‐based detection of C. burnetii in raw milk samples from cows sold in Brazil that do not undergo official inspection or pasteurization.     

Meta‐analysis of the prevalence of thermotolerant Campylobacter in food‐producing animals worldwide

The objective of this meta‐analysis was to summarize available information on the prevalence of thermotolerant Campylobacter (TC) in different food‐producing animals worldwide. Databases (i.e., PubMed, ScienceDirect, Scopus) were searched from 1980 to 2017 unrestricted by language. The inclusion criteria were as follows: prevalence or incidence studies, published in peer‐reviewed journals, and they must have reported the total number of animal samples studied and the number of samples that were positive for the presence of TC. When the identification of Campylobacter species was available, this information was included in the analysis. Multilevel random‐effect meta‐analysis models were fitted to estimate mean occurrence rate of TC and to compare them among different factors potentially associated with the outcome. The mean occurrence rate of TC in food‐producing animals was 0.424 (95% CI: 0.394–0.455), and the mean occurrence rate of Campylobacter jejuni and Campylobacter coli were 0.214 and 0.133, respectively. Pigs and poultry showed the highest prevalence of TC; however, there were differences in the prevalence of each Campylobacter species. Campylobacter jejuni was observed in broilers (0.322; 95% CI: 0.273–0.377) and hens (0.395; 95% CI: 0.265–0.542), while C. coli was restricted essentially in pigs (0.553; 95% CI: 0.541–0.650). The prevalence of C. jejuni in intensively bred cattle was higher (0.302; 95% CI: 0.227–0.389) than the prevalence in extensively bred cattle (0.172; 95% CI: 0.119–0.242) while the prevalence of C. coli was similar (0.051; 95% CI: 0.028–0.091 vs. 0.050; 95% CI: 0.027–0.091) in both production systems. Agar with or without blood used for the isolation of TC did not affect the prevalence observed. The method of species identification did not seem to generate differences in the prevalence of Campylobacter species. The prevalence of Campylobacter in primary food production has a strong impact on the entire agri‐food chain. National authorities must monitor the situation with the aim to establish the appropriate risk management measures.     

Minimum number of measurements for efficient estimation of black spot resistance in papaya genotypes

European Journal of Plant Pathology - During a field survey in Tamil Nadu (2014–16), chilli plants showing chlorotic and necrotic ring spots and necrotic streaks on leaves and stems were...     

Effect of Nitrogen and Trinexapac-Ethyl Rates on the SPAD Index of Wheat Leaves

ABSTRACT

The objective of this study was to evaluate the effect of nitrogen (N) and trinexapac-ethyl (TE) rates on the SPAD index in wheat flag leaf. The treatments were five N rates (30, 60, 90, 120, 150 kg ha^?1) combined with four TE rates (0, 63, 125, 188 g ha^?1). The experiment was carried out in a randomized block design with four repetitions. SPAD index, leaf N content and grain yield showed quadratic response to the increase in N rates, whereas area, wet and dry weight of flag leaf presented linear increase. TE caused linear increase in SPAD index, linear decrease in leaf area, reduction in grain yield with smaller N rates and increase with larger N rates. The N content, and the wet and dry weight of flag leaf were not affected by TE. SPAD calibration to estimate N status in wheat should be specific for each TE rate.     

Glycosaminoglycans from Litopenaeus vannamei Inhibit the Alzheimer’s Disease β Secretase,BACE1

Only palliative therapeutic options exist for the treatment of Alzheimer’s Disease; no new successful drug candidates have been developed in over 15 years. The widely used clinical anticoagulant heparin has been reported to exert beneficial effects through multiple pathophysiological pathways involved in the aetiology of Alzheimer’s Disease, for example, amyloid peptide production and clearance, tau phosphorylation, inflammation and oxidative stress. Despite the therapeutic potential of heparin as a multi-target drug for Alzheimer’s disease, the repurposing of pharmaceutical heparin is proscribed owing to the potent anticoagulant activity of this drug. Here, a heterogenous non-anticoagulant glycosaminoglycan extract, obtained from the shrimp Litopenaeus vannamei, was found to inhibit the key neuronal β-secretase, BACE1, displaying a more favorable therapeutic ratio compared to pharmaceutical heparin when anticoagulant activity is considered.     

Castor beans hulls as a replacement for Tifton 85 hay in lamb diets

The objective of this study was to evaluate the effect of replacing Tifton 85 hay (0, 33, 66, or 100 %) with by castor bean hulls on the intake and nutrient digestibility and performance of 28 noncastrated lambs, averaging 7 months in age, 19.5?±?2.0 kg average live weight, fed on diets based on forage cactus. The animals were housed in individual pens for 100 days. The digestibility trial occurred 40 days after the start of the performance using LIPE® as an external indicator for estimating fecal dry matter production. The replacement of hay with Castor beans hulls linearly decreased the intakes of dry matter, neutral detergent fiber, organic matter, and crude protein and reduced the digestibility coefficients of the dry matter, neutral detergent fiber, organic matter content, crude protein, and total digestible nutrient content. The hot carcass and true carcass yields were not influenced by the treatments and the feed conversion increased linearly. The empty body weight, live weight at slaughter, hot and cold carcass weights, total gain, and average daily gain decreased linearly with the replacement. The replacement of Tifton 85 hay with castor bean hulls reduced the intake of dry matter, crude protein and energy, consequently reducing the performance of the animals. However, as the regression coefficients were of low magnitude, data were subjected to analysis of variance and means were compared using the Dunnett test with diet without castor bean hulls set as the control treatment. The Tifton 85 hay can be replaced up to 66 % by castor bean hulls in finishing diets for lambs.     

猪链球菌是常见的共栖菌

猪链球菌感染常常很复杂 ,因为猪的扁桃体内和鼻腔中存在多种不同的血清型和不同的菌株。必须用与所见临床病例有关的特定菌株进行流行病学研究。用新的分子生物学技术来对同一血清型的不同菌株进行比较 ,必然很快就能使我们更充分地认识这一疾病