The economic viability for the production of live baits of White Shrimp (Litopenaeus schmitti) in recirculation culture system

Sportfishing is a growing segment in the fishing sector, which requires the supply of marine shrimps to meet the demand for live baits; however, the extraction of white shrimp from their natural habitat results in overfishing depleting natural stocks. Intensive cultivation in water recirculation system is a worldwide trend for shrimp farming, which, in addition to increasing productivity, minimizes the impact of effluent emission on the environment, reinforcing sustainability. This study analyzed the economic viability of establishing farms for the cultivation of white shrimp (Litopenaeus schmitti) in water recirculation system to meet the market demand of sportfishing for live baits. The indicators were used: internal rate of return (IRR), net present value (NPV) and payback period (PP). The best scenario, with 90 % survival rate and unitary selling prices of US$ 0.50, showed IRR 11.74 %, NPV (10 %) US$ 1,241.88 and PP 5.71 years, demonstrating financial risk for the production of live baits in the conditions proposed in this study.     

Phenolic Compounds from Maté (Ilex paraguariensis) Inhibit Adipogenesis in 3T3-L1 Preadipocytes

Leaves of Ilex paraguariensis are used to prepare a tea known as maté which is a common beverage in several South American countries. The ethanol extract was fractionated to identify the compounds responsible for the anti-adipogenic activity in 3T3-L1 cells. Extracts of both fresh and dried maté leaves were subjected to column chromatography using molecular permeation to obtain the saponin (20?% yields) and the polyphenol extracts (40?% yields) from the fresh and dried leaves. The phenolic content was determined using high-performance liquid chromatography analysis and the Folin-Ciocalteau method. Also, maté extracts (50?μg/ml to 1,000?μg/ml) did not display citotoxicity using MTT. The polyphenol extract from the dried leaves was the most effective (50?μg/ml) in the inhibition of triglyceride accumulation in 3T3-L1 adipocytes, and rutin (100?μg/ml) likely accounted for a large portion of this activity. Additionally, maté extracts had a modulatory effect on the expression of genes related to the adipogenesis as PPARγ2, leptin, TNF-α and C/EBPα.     

Evaluation of albuminuria and its relationship with blood pressure in dogs with chronic kidney disease

Background: Microalbuminuria and hypertension have long been associated with a guarded prognosis in human patients with a variety of diseases. In veterinary medicine, tests for microalbuminuria have been used for detecting early kidney damage, but there is little information regarding its association with high blood pressure in dogs with chronic kidney disease (CKD). Objective: The objective of this study was to evaluate albuminuria and its association with arterial hypertension in dogs with CKD. Methods: Urinary albumin:creatinine (UAC) ratio, urinary protein:creatinine (UPC) ratio, and systolic blood pressure were determined in 39 clinically healthy dogs and 40 dogs with CKD. Results: UAC in dogs with CKD (range, 0.002–7.99; median, 0.38) was statistically different from that of control dogs (range, 0.0005–0.01; median, 0.002). Microalbuminuria (UAC 0.03–0.3) and macroalbuminuria (UAC>0.3) were detected in 32.5% and 50% of dogs with CKD, respectively. Sixty percent (24/40) of dogs with CKD had systolic pressure ≥180 mmHg; in these dogs, UAC ratio (range, 0.006–7.99; median, 1.72) was significantly higher than in dogs with CKD and systolic pressure<180 mmHg (range, 0.002–4.83; median, 0.10). Of hypertensive dogs with CKD, those with UPC>1.0 usually had macroalbuminuria, those with UPC 0.5–1.0 usually had microalbuminuria, and those with UPC<0.5 usually lacked albuminuria. Conclusions: UAC ratio was higher in hypertensive than in normotensive dogs with CKD. Tests designed to detect microalbuminuria may be useful for hypertensive dogs with CKD and a UPC≤1.0 to detect the onset and magnitude of albuminuria. Once macroalbuminuria is overt, the UPC ratio itself can be used for the same purpose.     

Osmotic adjustment,proline accumulation and cell membrane stability in leaves of Cocos nucifera submitted to drought stress

The role of drought-induced proline accumulation in coconut leaves is still unclear. With the objective of evaluating the impact of water shortage on leaf osmotic potential, proline accumulation and cell membrane stability in young plants of two Brazilian Green Dwarf coconut ecotypes from contrasting areas (Brazilian Green Dwarf from Una, Bahia, UGD, and from Jiqui, Rio Grande do Norte, JGD), a pot experiment was conducted under greenhouse conditions. Three drought cycles consisting of suspension of irrigation until the net photosynthetic rate (A) approached zero and rewatering until recovery of A to 85% of the irrigated control plants. Pre-dawn leaf water potential (Ψ_PD) reached −1.2 MPa at the point of maximum stress (PMS). Dry matter production and leaf area were severely reduced by drought treatment in the two ecotypes. Corrected values of osmotic potential were significantly reduced in stressed plants of the two ecotypes. Green dwarf coconut palm showed low osmotic adjustment (from 0.05 to 0.24 MPa) and significant accumulation of proline (from 1.5 to 2.1 times in relation to control) in leaflets in response to water deficit. Considering the growth reduction observed in both ecotypes, proline was not associated to osmoregulation. On the other hand, the absence of membrane damage, as indicated by electrolyte leakage method, suggests that the protective role of proline in this specie can be more important. The two ecotypes of Green dwarf coconut palm behaved similarly in the present experiment for most traits evaluated. Slight differences among the ecotypes were observed with respect to the response to treatments, such as higher proline accumulation in JGD.     

Soil chemical attributes,nutrient uptake and yield of no-till crops as affected by phosphogypsum doses and parceling in southern Brazil

This study evaluated soil chemical attributes, leaf nutrient concentrations and grain yield of corn (Zea mays), barley (Hordeum vulgare), common bean (Phaseolus vulgaris) and wheat (Triticum aestivum) as affected by phosphogypsum (PG) rates and split application to a Typic Hapludox under no-till from Southern Brazil. A Randomized complete block design was used, with factorial treatment structure (5 x 2) consisting of increasing PG rates (0, 3, 6, 9 and 12 Mg ha^?1) either in single or split (half dose, two consecutive years) application. PG rates have reduced concentrations of Al³⁺, increasing Ca²⁺, SO₄^2- and pH values in soil layers up to 0.8 m. Leaf concentrations of Ca (between 28 and 42%) and S (between 6% and to 50%) increased in all crops, while leaf levels of Mg decreased on corn (?16%), bean (?22%) and wheat (?14%) by PG rates. Compared to the control, PG rates between 4.0 and 6.1 Mg ha^?1 promoted increases of 11%, 10% and 10% on corn, barley and wheat yields, respectively. There was no effect of PG on common bean’s yield. Phosphogypsum was effective for improving soil fertility, plant nutrition and crop yields. Applying the PG annually at one-half rate gave best overall results.     

Use of Moringa oleifera Seed as a Natural Adsorbent for Wastewater Treatment

Moringa oleifera (MO) is a multipurpose, medium- or small-sized tree, from regions of north-west India and indigenous to many parts of Asia, Africa, and South America. Its pods have been employed as an inexpensive and effective sorbent for the removal of organics, and coagulant for water treatment. It is a non-toxic natural organic polymer. The main objective of this work was to use the MO seeds as a natural adsorbent for the treatment of dairy industry wastewater (DIW). The effects of agitation time, pH, MO biomass dose, and DIW concentration were evaluated. Removal efficiencies of up to 98%, for both color and turbidity, were reached using 0.2 g MO and 0.2 L of 1.0 g/L sorbate solution (DIW). The obtained results showed that MO seed keeps its adsorption power under a pH range between 5 and 8. The adsorption data was fitted to Langmuir isotherm. There was a significant uptake capacity of MO biomass, q _max, which suggested a good affinity between DIW components and sorbent. We conclude that the MO biomass has the potential to be used in the dairy industry wastewater treatment in an efficient way and with low cost.     

Geostatistical analysis of fruit yield and detachment force in coffee

The aim of this study was to use geostatistical analysis to evaluate the spatial variation in the detachment force of coffee fruit and coffee yield by variograms and kriging for precision agriculture. This study was conducted at Brej?o farm, Três Pontas, Minas Gerais, Brazil. The detachment force of green and mature coffee fruit was measured with a prototype dynamometer and georeferenced. The yield data were obtained from manual harvesting and were georeferenced. The data were evaluated by variograms estimated by residual maximum likelihood (REML), which provided a satisfactory approach for modeling all the variables with a small sample size. Spherical and exponential models were fitted, the first provided the better fit to mature fruit detachment force and the latter provided the better fit to coffee yield and green fruit detachment force. They were used to describe the structure and magnitude of spatial variation in the variables studied. Kriged estimates were obtained with the best fitting variogram models and mapped. The statistical and geostatistical analyses enabled us to characterize the spatial variation of the detachment force of green and mature coffee fruit and coffee yield and to visualize the spatial relations among these variables. The precision agriculture techniques used in this paper to collect, map and analyze the variables studied will help coffee farmers to manage their fields. Maps of coffee yield will enable farmers to apply nutrients site-specifically and manage harvesting either manually or mechanically. In addition, maps of detachment force of coffee fruit can enable farmers to harvest coffee selectively by choosing the appropriate places and the right time to start. This will improve the quality of the final product and also increase profits.     

Optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate Haemophilus parasuis,Streptococcus suis,and Mycoplasma hyorhinis in formalin-fixed,paraffin-embedded tissues from pigs with polyserositis

An optimized protocol was developed for the simultaneous detection and differentiation of Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded (FFPE) tissues with multiplex nested polymerase chain reaction (PCR). This method also determines the prevalence of these bacteria in pigs with polyserositis. DNA extraction with a combination of a commercial reagent and proteinase K resulted in more frequent detection of the pathogens than DNA extraction with proteinase K alone. Among FFPE tissue samples from 312 cases of polyserositis in which at least 1 bacterial species was detected, multiplex nested PCR detected H. parasuis in 239 (77%), S. suis in 124 (40%), and M. hyorhinis in 40 (13%). The disease was caused by a single pathogen in 224 (72%) of the cases and multiple pathogens in 88 (28%). Among the pigs positive for H. parasuis, S. suis, and M. hyorhinis by multiplex nested PCR, the pathogen was isolated from only 11%, 35%, and 28%, respectively. Therefore, the PCR protocol developed in this study is a useful diagnostic method when samples are negative after isolation methods and even for samples in which only 1 pathogen was isolated.