全文获取类型
收费全文 | 6533篇 |
免费 | 3358篇 |
国内免费 | 1篇 |
专业分类
林业 | 186篇 |
农学 | 434篇 |
基础科学 | 2篇 |
1319篇 | |
综合类 | 53篇 |
农作物 | 104篇 |
水产渔业 | 2507篇 |
畜牧兽医 | 4010篇 |
园艺 | 5篇 |
植物保护 | 1272篇 |
出版年
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 125篇 |
2020年 | 474篇 |
2019年 | 1028篇 |
2018年 | 897篇 |
2017年 | 938篇 |
2016年 | 929篇 |
2015年 | 816篇 |
2014年 | 800篇 |
2013年 | 971篇 |
2012年 | 459篇 |
2011年 | 470篇 |
2010年 | 600篇 |
2009年 | 253篇 |
2008年 | 231篇 |
2007年 | 71篇 |
2006年 | 104篇 |
2005年 | 96篇 |
2004年 | 98篇 |
2003年 | 93篇 |
2002年 | 110篇 |
2001年 | 72篇 |
2000年 | 105篇 |
1999年 | 15篇 |
1997年 | 16篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 8篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1979年 | 3篇 |
1978年 | 7篇 |
1977年 | 7篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1970年 | 5篇 |
1968年 | 3篇 |
1926年 | 1篇 |
1869年 | 1篇 |
排序方式: 共有9892条查询结果,搜索用时 0 毫秒
81.
J. Jiang L. Feng Y. Liu W.‐D. Jiang K. Hu S.‐H. Li X.‐Q. Zhou 《Aquaculture Nutrition》2014,20(6):731-740
Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln. 相似文献
82.
Intraguild predation of Geocoris punctipes on Eretmocerus eremicus and its influence on the control of the whitefly Trialeurodes vaporariorum 下载免费PDF全文
83.
84.
The insecticidal activities of materials derived from the fruit of fennel, Foeniculum vulgare, against adults of Sitophilus oryzae, Callosobruchus chinensis and Lasioderma serricorne were examined using direct contact application and fumigation methods. The biologically active constituents of the Foeniculum fruits were characterized as the phenylpropenes (E)‐anethole and estragole, and the monoterpene (+)‐fenchone, by spectroscopic analysis. Responses varied with insect species, compound, dose and exposure time. In a filter paper diffusion test, estragole at 0.168 mg cm?2 caused 91% mortality to S oryzae adults within 1 day after treatment (DAT), whereas (+)‐fenchone and (E)‐anethole gave over 90% mortality at 2 and 4 DAT, respectively. Against C chinensis adults, all test compounds revealed potent insecticidal activities at 0.021 mg cm?2 at 2 DAT. Against L serricorne adults at 0.105 mg cm?2, (E)‐anethole gave 100% mortality at 1 DAT, whereas 90 and 60% mortality at 4 DAT was achieved with estragole and (+)‐fenchone, respectively. In a fumigation test, the test compounds were much more effective against adults of S oryzae, C chinensis and L serricorne in closed cups than in open ones, indicating that the insecticidal activity of test compounds was largely attributable to fumigant action. As naturally occurring insect‐control agents, the F vulgare fruit‐derived materials described could be useful for managing field populations of S oryzae, C chinensis and L serricorne. © 2001 Society of Chemical Industry 相似文献
85.
Xenotransfusion of anemic cats with blood compatibility issues: pre‐ and posttransfusion laboratory diagnostic and crossmatching studies 下载免费PDF全文
86.
Applications of ionizing radiation for the control of postharvest diseases in fresh produce: recent advances 下载免费PDF全文
Postharvest diseases cause considerable losses to harvested fruits and vegetables worldwide. In addition to various treatments to control postharvest losses caused by pathogens, the global trend is shifting toward more ecofriendly alternatives safer to human health. Thus far, the main approach to postharvest disease control has mostly relied on the use of chemicals. However, the intense use of chemical fungicides has caused side effects such as environmental issues and evolution of fungicide‐resistant isolates. With increasing demand for the use of non‐chemicals, ionizing radiation has been investigated for application in many aspects of postharvest treatment, especially as an approach with significant potential for the control of postharvest disease. Recently, a number of more or less technologically advanced methodologies, e.g. irradiation combined with other types of treatments and induced disease‐resistance, have been developed to control postharvest diseases as well as to increase the quality and storage life of fresh commodities. Challenges for future application of irradiation in fresh produce are the cost, lack of irradiation facilities, lack of knowledge about optimal conditions for different commodities, and lack of acceptance of irradiated fresh produce. This review aims to document the advances in understanding of the effects of irradiation on postharvest disease, the possible modes of action, and the perspectives in commercial use. 相似文献
87.
Autochthonous Chagas disease in the southern United States: A case report of suspected residential and military exposures 下载免费PDF全文
N. Harris L. Woc‐Colburn S. M. Gunter R. Gorchakov K. O. Murray S. Rossmann M. N. Garcia 《Zoonoses and public health》2017,64(6):491-493
Chagas disease is a parasitic infection that can result in a progressive dilated cardiomyopathy. Here, we present the epidemiologic details of a suspected locally acquired transmission case originating from the southern United States. This is the first published report of Chagas disease in a young, healthy United States veteran with repeat triatomine exposures in Arizona. Military personnel and Arizona residents should be aware of their Chagas disease transmission risks. 相似文献
88.
Ali Murtaza Waqas Ahmad Tariq Sohail Muhammad Irfan‐ur‐Rehman Khan Imran Mohsin Muhammad Shahzad Mujahid Hussain Muhammad Zahid Tahir Muhammad Ijaz 《Reproduction in domestic animals》2019,54(3):545-550
This study compares the factors associated with variable interval to oestrus and ovulation between early versus late ovulating goats following PGF2α administration. The time of ovulation in Beetal goats (n = 38) was monitored through transrectal ultrasound at every 6 hr following a single dose of PGF2α (experiment 1). Variations in oestrus and ovulation times were further explored through the changes in follicular dynamics, endocrine profiles and behaviour in another set of goats (n = 13) following single PGF2α given randomly during the luteal phase (experiment 2). The ovulation time varied between 60 and 96 hr, and 57% of ovulations occurred by 72 hr following PGF2α (experiment 1). Accordingly, the goats (n = 13) in the second experiment were retrospectively divided either into early and/or late ovulating, that is, ≤72 and/or ≥84 hr following PGF2α. The onset of oestrus, peak estradiol‐17β concentration and LH surge after PGF2α was first observed in early than late ovulating goats (p < 0.05). The goats ovulating early had larger follicle and smaller CL in diameter at the time of PGF2α administration than those ovulating late (5.4 ± 0.2 vs. 4.3 ± 0.2 mm and 10 ± 0.6 vs. 11.8 ± 0.3 mm, respectively; p < 0.05). Likewise, plasma progesterone concentration tended to be lower (p = 0.087) in early than late ovulating goats. In conclusion, the size of dominant follicle and CL at the time of PGF2a determines the interval to ovulation following a single dose of PGF2a during the luteal phase. 相似文献
89.
90.
Combined Campylobacter jejuni and Campylobacter coli Rapid Testing and Molecular Epidemiology in Conventional Broiler Flocks 下载免费PDF全文
G. Schallegger S. Muri‐Klinger K. Brugger C. Lindhardt L. John M. Glatzl M. Wagner B. Stessl 《Zoonoses and public health》2016,63(8):588-599
Campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. From an epidemiological and risk assessment perspective, further knowledge should be obtained on Campylobacter prevalence and genotype distribution in primary production. Consequently, 15 Austrian broiler flocks were surveyed in summer for their thermophilic Campylobacter spp. contamination status. Chicken droppings, dust and drinking water samples were collected from each flock at three separate sampling periods. Isolates were confirmed by PCR and subtyped. We also compared three alternative methods (culture‐based enrichment in Bolton broth, culture‐independent real‐time PCR and a lateral‐flow test) for their applicability in chicken droppings. Twelve flocks were found to be positive for thermophilic Campylobacter spp. during the entire sampling period. Seven flocks (46.6%) were contaminated with both, C. jejuni and C. coli, five flocks harboured solely one species. We observed to a majority flock‐specific C. jejuni and C. coli genotypes, which dominated the respective flock. Flocks within a distance <2 km shared the same C. jejuni genotypes indicating a cross‐contamination event via the environment or personnel vectors. Multilocus sequence typing (MLST) of C. jejuni revealed that the majority of isolates were assigned to globally distributed clonal complexes or had a strong link to the human interface (CC ST‐446 and ST4373). The combination of techniques poses an advantage over risk assessment studies based on cultures alone, as, in the case of Campylobacter, occurrence of a high variety of genotypes might be present among a broiler flock. We suggest applying the lateral‐flow test under field conditions to identify ‘high‐shedding’ broiler flocks at the farm level. Consequently, poultry farmers and veterinarians could improve hygiene measurements and direct sanitation activities, especially during the thinning period. Ultimately, real‐time PCR could be applied to quantify Campylobacter spp. directly from chicken droppings and avoid non‐interpretable results achieved by culture‐dependent methods. 相似文献