Nitric oxide level in seminal plasma and its relation with sperm DNA damages

BACKGROUND: Evidence supports the involvement of nitric oxide (NO) in a variety of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established that high amounts of NO have detrimental effects on DNA. The integrity of sperm DNA is an important factor in successful fertility and embryo development. It is hypothesized that supra physiological concentrations of NO in seminal plasma cause sperm DNA damage. The aim of this study was to determine sperm DNA damage by comet assay and its correlation with NO level in seminal plasma of fertile and infertile men. METHODS: Semen samples were collected from 45 patients and 70 healthy donors. The stable metabolites of NO (nitrite and nitrate) in seminal plasma were measured by Griess assay and DNA damage was determined using single cell gel electrophoresis (comet) assay method. RESULTS: The NO concentration in the seminal plasma of infertile males was significantly higher than fertile males (5.74+/-1.01 microM/L vs. 3.88+/-0.53 microM/L). There was a significant positive correlation between the NO concentration and sperm DNA comet value in infertile males (P<0.01, R=0.598). CONCLUSION: These results indicate that the overproduction of NO in genital tract of infertile males has a potential pathogenetic role in the reduction of sperm DNA integrity.     

Impregnation of ethylcellulose microcapsules containing jojoba oil onto compressive knits developed for high burns

The medical uses of textile fibers and fabric forms keep on growing. Today, the knits can be used in the medical field. The aim of this present work was to prepare compressive knits, impregnated by ethylcellulose (EC) microcapsules, for the burnt skin. Ethylcellulose microcapsules containing jojoba oil were prepared via the phase separation method. Jojoba oil was chosen because it plays an essential role in skin hydration and avoids the sebum accumulation. Ethylcellulose microcapsules obtained were grafted on two different compressive knit surfaces by impregnation, using the Acrylic Resin (AR) as binder. The prepared microcapsules were analyzed by Scanning Electron Microscopy (SEM). The stability of the microcapsules, the efficiency of the microencapsulation method and the jojoba oil content determination were also investigated. This method of application improved the fabric surface and permitted in maximum the preserving of initial characteristics of knit, such as touch, flexibility and lightness.     

CD226 rs763361 (Gly307Ser) Polymorphism Is Associated with Susceptibility to Rheumatoid Arthritis in Zahedan,Southeast Iran

Background: Rheumatoid arthritis (RA) is a chronic inflammatory disease with many genetic factors predisposing to disease susceptibility. The aim of the present study was to investigate the impact of CD226 rs727088 and rs763361 polymorphisms and susceptibility to RA in a sample of the Iranian population. Methods: This case-control study was carried out on 100 patients with RA and 104 healthy subjects. The polymorphisms were determined using tetra amplification refractory mutation system-polymerase chain reaction assay. Results: The rs763361 (Gly307Ser) polymorphism increased the risk of RA in codominant, dominant and recessive-tested inheritance models (odds ratio [OR] = 3.18, 95% confidence intervals [95% CI] = 1.44-7.02, P = 0.004, CC vs. TT, and OR = 1.98, 95% CI = 1.10-3.57, P = 0.023, CC vs. CT-TT, and OR = 2.61, 95% CI = 1.26-5.37, P = 0.010, CC + CT vs. TT, respectively). In addition, the rs763361 T allele increased the risk of RA (OR = 2.06, 95% CI = 1.38-3.08, P<0.001). However, no significant difference was observed among the groups regarding CD226 rs727088 polymorphism (χ2 = 3.20, P = 0.202). Conclusions: Our finding showed that CD226 rs763361, but not rs727088, gene polymorphism increased the risk of RA in a sample of the Iranian population.Key Words: Rheumatoid arthritis (RA), CD226, Polymorphism     

Preparation of magnetic and conductive graphite nanoflakes/SrFe<Subscript>12</Subscript>O<Subscript>19</Subscript>/polythiophene nanofiber-nanocomposites and its radar absorbing application

This study, we synthesized graphite-nanoflakes (GNFs) by acid treatment and thermal shock and then using the ultrasonic irradiation technique to exfoliate flake-carbon. The SrFe₁₂O₁₉ nanoparticles (NPs) were coated by co-precipitate method on GNFs after by alkaline treatment. Finally nanocomposite (GNF/SrFe₁₂O₁₉/PTh) was prepared by in-situ oxidative polymerization method in presence of thiophene (Th) as monomer. The magnetic and electrical conducting properties of the resulting nanocomposites were measured by using vibrating sample magnetometer and standard four-point-probe method, respectively. The synthesized nanocomposites were characterized by X-ray diffraction (XRD) and fourier transform infrared spectra (FTIR). In addition, morphological analyses were investigated by scanning electron microscopy (SEM). A minimum reflection loss (RL) of GNFs/SrFe₁₂O₁₉/PTh with 50 % wt GNFs/SrFe₁₂O₁₉ as core were observed ?28 and ?39 dB at 9.7 and 12 GHz for a 1.5 mm thickness. The results indicated that we can perform good microwave shielding in X-band (8–12 GHz) by these nanocomposites.     

Electrospinning of silk nanofibers. I. An investigation of nanofiber morphology and process optimization using response surface methodology

Ultra fine fibers were electrospun from regenerated silk fibroin/formic acid solution. Effect of some process parameters on the morphology, diameter and variation in fiber diameter of electrospun fibers were experimentally investigated. Scanning electron microscope was used for the measurement of fiber diameter. Fibers with diameter ranging from 80 to 210 nm were collected depending on the solution concentration and the applied voltages. Response surface methodology (RSM) was used to obtain a quantitative relationship between selected electrospinning parameters and the average fiber diameters and its distribution. It was shown that concentration of silk fibroin solution had a significant effect on the fiber diameter and the standard deviation of the fiber diameter. Applied voltage had no significant effect on the fiber diameter and its standard deviation.     

Preliminary study of the performance of crude illipe oil (Shorea macrophylla) as a dietary lipid source for riverine cyprinid Tor tambroides

Fisheries Science - Illipe Shorea macrophylla is a riverine fruit naturally eaten by Malaysian mahseer Tor tambroides and other riverine cyprinids. The effects of crude illipe oil on the growth...     

Strip-tillage effect on seedbed soil temperature and other soil physical properties

The no-tillage system is perceived as having lower soil temperatures, wetter soil conditions, and greater surface penetration resistance compared with conventional and other conservation tillage systems. Concerns associated with the effect of the no-tillage system on certain soil physical properties (i.e. soil temperature, moisture, and compaction) prompted this study to evaluate the effect of an alternative tillage system, strip-tillage, on these physical properties, compared with chisel plow and no-tillage systems. The study was conducted on two Iowa State University research and demonstration farms in 2001 and 2002. One site was at the Marsden Farm near Ames, where the soils were Nicollet loam (Aquic Hapludolls) and Webster silty clay loam (Typic Haplaquolls). The second site was at the Northeast Research and Demonstration Farm near Nashua, where the soils were Kenyon loam (Typic Hapludolls) and Floyd loam (Aquic Hapludolls).Soil temperature increased in the top 5 cm under strip-tillage (1.2–1.4 °C) over no-tillage and it remained close to the chisel plow soil temperature. This increase in soil temperature contributed to an improvement in plant emergence rate index (ERI) under strip-tillage compared with no-tillage. The results show no significant differences in soil moisture status between the three tillage systems, although the strip-tillage soil profile has slightly greater moisture content than chisel plow. Moisture content through the soil profile particularly at the lower depths under all tillage treatments was greater than the plant available water (PAW). However, the changes in soil moisture storage were much greater with strip-tillage and chisel plow than no-tillage from post-emergence to preharvest at 0–30 and 0–120 cm. It was observed also that most change in soil moisture storage occurred between post-emergence and tasseling. Penetration resistance was similar for both strip-tillage and no-tillage, but commonly greater than chisel plow. In general, the findings show that strip-tillage can contribute effectively to improve plant emergence, similar to chisel plowing and conserve soil moisture effectively compared with no-tillage.     

Molecular diagnostics for the sigatoka disease complex of banana

ABSTRACT The Sigatoka disease complex of banana involves three related ascomycetous fungi, Mycosphaerella fijiensis, M. musicola, and M. eumusae. The exact distribution of these three species and their disease epidemiology remain unclear, because their symptoms and life cycles are rather similar. Disease diagnosis in the Mycosphaerella complex of banana is based on the presence of host symptoms and fungal fruiting structures, which hamper preventive management strategies. In the present study, we have developed rapid and robust species-specific molecular-based diagnostic tools for detection and quantification of M. fijiensis, M. musicola, and M. eumusae. Conventional species-specific polymerase chain reaction (PCR) primers were developed based on the actin gene that detected DNA at as little as 100, 1, and 10 pg/mul from M. fijiensis, M. musicola, and M. eumusae, respectively. Furthermore, TaqMan real-time quantitative PCR assays were developed based on the beta-tubulin gene and detected quantities of DNA as low as 1 pg/mul for each Mycosphaerella sp. from pure cultures and DNA at 1.6 pg/mul per milligram of dry leaf tissue for M. fijiensis that was validated using naturally infected banana leaves.