不同栽植密度对辣椒产量的影响研究

为进一步提高毕节地区名优辣椒的种植水平,开展不同栽植密度对金椒1号和黔西泡通椒产量影响的研究试验,结果表明:金椒1号、黔西泡通椒栽植密度分别为8 596、8 748株/666.67 m2时,最高产量分别可达225.55、248.17 kg/667.67 m2,经济效益最好。     

花生组培苗嫁接技术的研究

为解决花生组培再生苗及转基因苗驯化移栽成活率低的难题,本试验以花生实生苗作为砧木,对花生嫁接技术进行了研究。试验结果表明:超净台内无菌嫁接效果较好,以再生苗或实生苗为接穗进行无菌嫁接,其成活率均达90%以上,明显高于室内嫁接成活率(71%～72%);无菌嫁接时12～15d苗龄的砧木效果最好;将无菌嫁接苗在培养基中培养3～5d后进行驯化移栽为最佳时期;不同花生基因型嫁接苗成活率在87%～95%;嫁接苗移栽田间后,其成活率高达94%,且100%结果。     

花溪灰萝卜区域试验研究

为筛选出具有推广潜力、产量较高、抗逆性强并适应贵州气候条件的饲用灰萝卜新品种,对3个灰萝卜品种的生育期、生产性能、抗逆性等进行了对比分析,结果表明,花溪灰萝卜品种优势突出,表现为产量高、抗逆性强,具有较大的推广潜力.     

TGF β1 inhibits the maturation of dendritic cells and down-regulates TLR4 expression

AIM: To investigate the effects of transforming growth factor β1 (TGF-β1) on murine-derived dendritic cells (DC). METHODS: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF β-DC. Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method. IL-12 p70 protein was detected by ELISA and the expressions of Toll like receptor 4 (TLR4) on DCs were measured by semi-quantitative RT-PCR and FCM. RESULTS: Compared to immature DC (imDC) cultured with GM-CSF alone, the expressions of CD80, CD86, I-Ab and CD40 in TGF β-DC were lower. The TGF β-DC was resistant to maturation by LPS. Maturation resistance was evident from a failure to up-regulate CMs, to stimulate larger T cell proliferation and to increase secretion of IL-12 p70. Down-regulation of TLR4 expression on TGF β-DC was also found. CONCLUSION: TGF-β1 inhibits the expression of co-stimulatory molecules on DC. It is resistant to maturation stimulus (LPS) and might be linked with TLR4 down-regulation.     

牛磺酸的生物学功能及其在蛋鸡生产中的研究应用

牛磺酸是一种具有广泛生物学功能的营养物质,它在畜牧业生产中的应用已引起人们的密切关注,成为当今研究的一个亮点。本文概述了牛磺酸的结构、性质、分布、合成代谢、生物学功能及其在蛋鸡生产中的研究应用进展,并指出了今后需解决的问题和发展前景。     

序贯培养法对猪孤雌激活胚胎发育能力的影响

经体外成熟、孤雌激活和培养获得猪胚胎,研究了不同培养体系、共培养体细胞和序贯培养对猪孤雌激活胚胎发育的影响。试验表明:孤雌激活卵母细胞在SOF 10?S培养体系中分裂效果最好,添加胎牛血清的NCSU-23和颗粒细胞对胚胎发育有促进作用,培养6d后发育到桑囊胚的比率增加(P<0.05)。在序贯培养的前3d,SOF培养基(不含葡萄糖)和颗粒细胞对胚胎的发育有促进作用,分裂率(P<0.05)和突破4细胞阻滞的数目显著增加,在培养的后3d,添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胚胎发育到桑囊胚,桑囊胚的发育率为(59.5±3.2)%(P<0.05)。结果表明,SOF培养基和颗粒细胞 添加胎牛血清的NCSU-23和输卵管上皮细胞的序贯培养系统能较好的促进胚胎的发育。     

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.     

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in β-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were β cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of β cell damage in diabetes probably through the mechanism of energy metabolism disturbance.     

双向电泳结合蛋白质印迹技术分析猪囊尾蚴乳酸脱氢酶

为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。     

南宁市花境植物引种选育研究探讨

对南宁花境植物引种选育状况进行分析,同时通过对南宁嘉和城的花境植物种类实地配置观察,选择出适应于南宁生长的花境品种62种.在分析南宁花境植物引种选育存在问题后提出建议.