Paleoclimatic implications of micromorphic features of Quaternary paleosols of NW Himalayas and polygenetic soils of the Gangetic Plains — A comparative study

Micromorphological examination of the paleosols (50–10 ka) developed in alluvial fan deposits of the NW Himalayas and the bordering polygenetic soils (mainly Holocene) of the Gangetic Plains has been used to differentiate the pedosedimentary features indicating climatic changes during late Quaternary time. The paleosols within rapidly aggrading sediments of the alluvial fans of the Dehradun valley resulted in response to the reduced rate of sedimentation and climatic changes and correspond to the MIS3 and MIS2 stages. Distinctive micromorphic features of these paleosols provided the details of the prevalent pedogenesis in response to the paleoclimatic changes during 50 ka. Microfabrics of these paleosols show reorganization of the pedality from massive and/or subangular blocky to platy and prismatic structures, strong to very strong mobilization of the plasma, different types of textural pedofeatures along with faunal activities. These pedofeatures are indicative of cold-humid climate with subsequent change to even colder but drier conditions during the last Glaciation. Comparison of the micromorphological characters of the paleosols of the NW Himalayas and the polygenetic soils of the Gangetic Plains show the same degree of soil development indicating 5–10 ka pedogenic intervals in alluvial fans of the Dehradun Valley. However, the difference in their pedofeatures is attributed to different pedogenic environments. The paleosols of the Dehradun Valley show predominance of the illuvial features with superposed impure silty clay on earlier clay pedofeatures and banded clay fabric features without any pedogenic calcium carbonate. The bordering Gangetic Plains are covered with polygenetic soils developed on stable surfaces and are < 13.5 ka. These surficial soils developed during the period marked by deglaciation and correspond to MIS1 stage. These are defined by the juxtaposition of different illuvial pedofeatures along with pedogenic calcium carbonate. This study suggests that formation of the paleosols in NW Himalayas was mainly controlled by warmer intervals during the last glaciation and the movement along the adjacent thrusts. While fluctuating climate punctuated with humid–semiarid–humid conditions played a major role during the formation of soils on the Gangetic Plains in Holocene that favoured illuviation, calcification and dissolution of pedogenic carbonates in the polygenetic soils.     

Classical swine fever in India: current status and future perspective

Classical swine fever (CSF) is a globally significant disease of swine caused by classical swine fever virus. The virus affects the wild boars and pigs of all age groups, leading to acute, chronic, late-onset or in-apparent course of the disease. The disease causes great economic loss to the piggery industry due to mortality, stunted growth, poor reproductive performance, and by impeding the international trade of pig and pig products. In India, CSF outbreaks are reported from most of the states wherever pig rearing is practiced and more frequently from northeast states. In spite of the highly devastating nature and frequent outbreaks, CSF remained underestimated and neglected for decades in India. The country requires rapid and sensitive diagnostic tests for an early detection of infection to limit the spread of the disease. Also, effective prophylactics are required to help in control and eradication of the disease for the development of the piggery industry. This review looks into the economic impact; epidemiology of CSF highlighting the temporal and spatial occurrence of outbreaks in the last two decades, circulation, and emergence of the virus genotypes in and around the country; and the constraints in the disease control, with the aim to update the knowledge of current status of the disease in India. The article also emphasizes the importance of the disease and the need to develop rapid specific diagnostics and effective measures to eradicate the disease.     

In Vitro Cytochrome P450 2E1 and 2A Activities in the Presence of Testicular Steroids

Cytochrome P450 2E1 (CYP2E1) and 2A (CYP2A) are the main enzymes involved in the metabolism of skatole in pigs. In this study, physiological concentrations of androstenone, 17β‐oestradiol and testosterone were tested for their ability to regulate CYP2E1 and CYP2A activity in liver microsomes isolated from entire male and female pigs as well as in microsomes from Saccharomyces cerevisiae expressing either human recombinant CYP2E1 or CYP2A6. We found that physiological concentrations of androstenone and oestradiol had the ability to inhibit CYP2E1 activity. The magnitude of this inhibition (approximately 30%) was similar in recombinant human CYP2E1 and microsomes from entire male pigs. This inhibition was only seen when adding the steroid to the assay 15 min before the substrate. Interestingly, CYP2E1 activity in the microsomes from female pigs was not affected. None of the investigated steroids modified the activity of recombinant human CYP2A6. However, CYP2A activity was slightly increased in the microsomes from female pigs in the presence of oestradiol, but the magnitude of this increase was very low (below 10%) and probably irrelevant. Overall, these results indicate that physiological concentrations of androstenone and oestradiol have a potential to inhibit CYP2E1 activities in vitro, and that this inhibition is gender‐specific. Further studies are needed to investigate the biochemical mechanisms underlying those differences between the genders.     

Experimental studies on immunosuppressive effects of peste des petits ruminants (PPR) virus in goats

Effect of virulent and attenuated peste des petits ruminants (PPR) virus on the immune response to nonspecific antigen (ovalbumin) was investigated. Clinical and serological responses were monitored in goats administered with ovalbumin concurrently with either PPR vaccine or virulent virus. Study showed that PPR virulent virus causes marked immunosuppression as evidenced by leukopenia, lymphopenia, and reduced early antibody response to both specific and nonspecific antigen. These observations were predominant particularly during acute phase of disease (4-10 days post-infection). On the other hand, the vaccine virus induced only a transient lymphopenia without significantly affecting the immune response to nonspecific antigen or to itself during this period. Further, the antibody levels to ovalbumin in the group administered with virulent PPRV increased significantly between days 28 and 35 post-infection in comparison to the titers in other two groups given with either ovalbumin alone or in combination with vaccine.     

Relationship between Oxygen Consumption and Sex of Bovine In Vitro Fertilized Embryos

The present study was conducted to examine the relationship between the oxygen consumption rate and sex ratio of bovine in vitro fertilized embryos on each day of blastocyst formation. The quality of blastocysts collected on day 7, 8, and 9 after in vitro fertilization (IVF) were categorized as ranks A and B (excellent and good, respectively) based on microscopic observation of the morphology. The oxygen consumption rate and sex of individual blastocysts were evaluated using two novel techniques: scanning electrochemical microscopy (SECM) and loop-mediated isothermal amplification (LAMP), respectively. The oxygen consumption rates of embryos of rank A were significantly higher (p < 0.05) than those of rank B, irrespective of the day of blastocyst appearance after IVF. Neither did the proportion of male embryos of ranks A and B differ significantly from each other at any of the days examined, nor from the average proportion (53%). The oxygen consumption rate of embryos of rank B collected on day 8 was significantly higher (p < 0.05) in female embryos than in male embryos collected on the same day. However, there were no apparent differences of oxygen consumption rates at each day of blastocyst appearance between male and female embryos of rank A. These results indicate that the oxygen consumption rate of individual embryos reflects their quality but does not correlate with the sex ratio of embryos of excellent quality.