我国三省区细粒棘球绦虫基因的变异分析

对从青海省、甘肃省和新疆维吾尔族自治区采集的24株细粒棘球蚴，用线粒体DNA的CO1基因和ND1基因结合rDNA的ITS2测序，调查了不同地区分离株基因型的变异情况。结果显示，所有分离株均为普通羊株(G1基因型)；CO1基因序列的变异率为0～0．8％，ND1基因的变异率为0．1％～0．7％；青海分离株ITS2序列的变异率为0．4％～3．1％；2株青海省西宁市和1株甘肃省武威市分离株分剐在C01基因和ND1基因序列不同位点发生的1处核苷酸非同义替换，导致1个编码的氨基酸替代。研究表明，我国上述3省、区部分区域存在的细粒棘球蚴属于同一株(G1基因型)。     

关于晚霜对桑树危害的情况分析及抗灾措施探讨

<正>霜冻是一种短时间的冻害,对桑树危害最严重的时期,主要是春季桑树萌芽后的晚霜,虽然有时仅仅几个小时,但危害极大。今年4月4日凌晨我市6700hm2桑园普受霜冻袭击,造成已萌发的桑芽枯干冻死,其危害为近40年不遇,我市广大蚕桑技术干部深入到田间调查研究,动员蚕农积极应对,采取扎实有效的措施,努力降低损失。     

剖腹产手术治疗母犬难产的体会

随着畜牧业的发展，人民生活水平的不断提高，宠物爱好者的增加，犬的饲养数量越来越大，母犬在产仔过程中遇到难产的现象，也就明显增加，笔者在日常门诊中曾多次遇到过此病例，对其中有的病例使用剖腹产术治疗难产，取得较好的疗效，现将治疗体会总结报告如下：     

家庭豆腐渣饲料的调制及其消化试验

豆腐渣营养价值较高,并且便宜,能使牛生产出优质肉。可是,最大的缺点是水分多,不利于长期贮存,易发霉变质。为了克服这一缺点,将其作为贮存饲料,进行了饲料化试验和消化试验。材料和方法用豆腐渣(水分75%)10、麦糠0.5、玉米0.5、大麦0.5、调配饲料0.5、稻秸1(单     

奶牛环形泰勒虫病的诊疗报告

奶牛环形泰勒虫病是一种严重危害奶牛养殖业的寄生虫病。它是由寄生在红细胞内的环形泰勒虫及其产生的毒素,导致脏器损伤,功能失调而引起的一种季节性很强的地方流行性寄生虫病。在我县黑帝村和查干村连续３年相继发生奶牛环形泰勒虫病。现将本病的诊疗过程报告如下。收...     

奶牛隐性乳房炎防治的新对策

奶牛乳房炎是奶牛的一种常见的多发病,分为临床型和亚临床型两大类。亚临床型又称为隐型乳房炎,其特征是乳房和乳汁无肉眼可见的异常变化,但乳汁在理化性质和细菌学方面发生了变化,     

Vaccine development for the prevention of staphylococcal mastitis in dairy cows

Staphylococci are the most common and costly mammary disease of dairy cattle worldwide. Target of RNAIII Activating Protein (TRAP), a membrane associated 167AA protein, is highly conserved among staphylococci and was shown in Staphylococcus aureus to be involved in bacterial stress response. The aims of this study were to test the safety and efficacy of recombinant TRAP (rTRAP) vaccine in dairy animals. The vaccine was safe as 2-3 subcutaneous injections of rTRAP (54-100 μg) with adjuvant ISA 206 to cows and goats did not lead to any abnormal symptoms of sensitivity to the vaccine. The rTRAP vaccine was immunogenic and caused the induction of a humoral immune response that remained high for at least 160 d post second immunization. The rTRAP vaccine was efficacious; at parturition only 13.5% (5/37) heifers in the immunized group were infected with Staphylococcus chromogenes as compared to 42.9% (18/42) in the non-immunized group. Additionally, when cows were immunized in mid-lactation, the difference between somatic cell count (SCC) in immunized and control animals was profound (45 ± 7 vs. 470 ± 194, respectively). At the same time, the difference in milk yield was also evident (48.3 ± 1.4 L d(-1)vs. 44.3 ± 0.9 L d(-1), respectively). Put together, these studies indicate the value of the rTRAP vaccine in preventing new udder infections by staphylococci, which significantly lead to lowered SCC and some increase in milk yield. TRAP is conserved among all strains and species and is constitutively expressed in any strain of S. aureus or CNS tested so far, including those isolated from cows. TRAP may thus serve as a universal anti-staphylococcus vaccine.