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991.
Breeding ewes in drylot were fed pelleted complete diets with 3% cottonseed meal (CSM) or 7% dried, gamma-irradiated sewage solids (DGSS) for 4 yr. Cytochrome P-450 (P-450) content and enzyme activities for xenobiotics biotransformations were assayed in livers after 2 yr and in livers, kidneys and ileal tissue after 4 yr. Dietary DGSS caused no increase in P-450 and few changes in activities of oxidative, hydrolative and conjugative biotransformational enzymes. Consumption of DGSS for 4 yr caused slight enlargement of spleens (1.1-fold, P less than .10) and ovaries (1.3-fold, P less than .10), but no change in size of livers, kidneys, hearts, adrenals and thyroids (P greater than .10), nor liver vitamin A levels (P greater than .10). Of 22 refractory lipophilic residues assayed in abdominal adipose tissue, few were detected and of those detected DGSS caused none to exceed normal levels. Dietary DGSS increased (P less than .01) Fe in livers 1.5-fold and in spleens 5.6-fold, and increased Cu in livers 1.3-fold (P less than .01) and in kidneys 1.2-fold (P less than .05). Dietary DGSS increased Cd levels in livers (P less than .01) but not in kidneys or spleens (P greater than .10); yet all Cd levels were within ranges for livestock fed conventional feed. Dietary DGSS caused no increase (P greater than .10) in levels of Ag, Ca, Cr, Hg, K, Mg, Mn, Na, Ni, P, Pb or Zn in livers, kidneys or spleens. There were no histopathological lesions of toxicosis except mild hemosiderosis of spleens. Consumption of a diet with 7% DGSS throughout 4 yr caused no hazardous accumulation of toxic elements and little, if any, evidence of toxicosis. 相似文献
992.
A microenzyme-linked immunosorbent assay (dot-ELISA) was modified for making an immunodiagnosis of Fasciola hepatica infections in sheep. Sheep were alloted as follows: group I-3 controls and 4 principals, each inoculated with 500 metacercariae; group II-3 controls and 7 principals, each inoculated with 250 metacercariae; and group III-3 controls and 7 principals, each inoculated with 500 metacercariae. Blood and fecal samples were collected from each animal every 2 weeks for 16 weeks. Presence (or absence) of flukes was confirmed by fecal examinations and examination of dissected livers at necropsy of the sheep. The dot-ELISA incubations were done at ambient room temperature. Nitrocellulose disks dotted with 1 microliter (50 ng of protein) of F hepatica excretory/secretory products were placed in 96-well tissue culture plates. After nonspecific binding sites on the disks were bound with bovine serum albumin-triethanolamine-buffered saline solution, dilutions (1:2) of positive- and negative-control serum samples or experimental serum samples were placed in appropriate wells for a 30-minute incubation. Wells were washed (3 times), and 50 microliters of horseradish peroxidase conjugated rabbit anti-sheep immunoglobulin G was added to each well for a 30-minute incubation and then aspirated. Substrate solution (4-chloro-1-naphthol, methanol, triethanolamine-buffered saline solution, and H2O2; 50 microliters) was added for a 30-minute incubation and then aspirated. Disks were air dried for visualization: solid purple dot = positive sample, or no dot = negative sample.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
993.
Data from 1,909 purebred, F1, backcross and F2 and F3 inter se combinations of Angus and Hereford were used to estimate average individual, maternal and grandmaternal genetic effects, individual and maternal heterosis, dominance and epistatic genetic effects. Models for evaluating heterosis and epistatic or recombination effects were discussed. Average individual effects indicate that Angus, compared with Hereford, had calves that were born earlier, had lighter birth weights, lower pre- and postweaning gains and lower pregnancy rates. Angus also produced lighter weight carcasses with more fat cover and marbling. Maternal effects of Angus were in the direction of reduced birth weight, greater calving ease, higher preweaning but lower postweaning growth rate and increased fatness when contrasted with Hereford. There was a tendency for opposite direction of maternal and grandmaternal effects for traits influenced by preweaning maternal environment. When additive X additive effects were ignored, total heterosis was significant for earlier day born, heavier birth weight, preweaning and postweaning gain, and heavier and fatter carcasses. Heterosis retained in F3 inter se vs F1 generation crosses indicated that net epistatic effects were relatively negligible for date of calving, birth weight, weaning gain and fat cover. There was a greater reduction of heterosis effects than expected from dominance alone for survival, pregnancy and marbling score. Loss of heterosis in F3 was less than expected for postweaning gain, carcass weight and rib eye area. Except for survival, pregnancy and marbling, these deviations from dominance expectations, or lack of them, are favorable for F3 composite populations. 相似文献
994.
Eight horses were allotted to 2 groups, each of 4 horses. All horses were given 100 plastic markers intragastrically via a nasogastric tube. One group of animals (control group) was not given medication after marker administration. The other group (test group) was given neostigmine methylsulfate (0.022 mg/kg of body weight) in the subcutaneous tissue at the time of marker administration and 30, 60, and 90 minutes later. All horses were killed 135 minutes after marker administration to locate the beads in the gastrointestinal tract. Gastric emptying of the markers was significantly delayed (P less than 0.05) in horses given neostigmine methylsulfate. 相似文献
995.
Investigations of toxicity episodes involving chemotherapeutic agents in Victorian poultry and pigeons 总被引:1,自引:0,他引:1
This series of case reports details observations on toxicity episodes in poultry due to a variety of chemotherapeutic agents. These problems arose owing to overdosage, variation in species susceptibility, potentiation of the toxic effects of one substance by the presence of another substance, and particular disease or other on-farm factors. Ignorance and accident were responsible for some of these situations. The episodes involved monensin, salinomycin, nicarbazin, sulphaquinoxaline, dinitolmide, dimetridazole, nitrofurans, streptomycin, and 3-nitro-4-hydroxyphenylarsonic acid. 相似文献
996.
997.
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999.
During October to December 1986, 365 traditional cattle herds in four provinces in Zambia were inspected and the owners interviewed. Information was collected on the treatment, management and disposal of cases of bovine dermatophilosis and on the effects of this disease on productivity for 1985. The contemporary financial cost per case of treatment and/or premature disposal, slaughter or death to the owners was K. 202 (US$ 91). The cost due to draft oxen being affected was estimated at K. 428 (US$ 193) per affected ox. The cost of reduced milk production, replacing affected cows and calf deaths, directly or indirectly from bovine dermatophilosis, was estimated at K. 132 (US$ 78) per affected milking cow. The total annual national cost of bovine dermatophilosis in 1985 was conservatively estimated to be some K. 6.9 million, (US$ 3 million). There were indications that the true financial cost in 1985 may have been up to 1.8 times the estimated cost. 相似文献
1000.
Persistence of avian influenza viruses in water 总被引:9,自引:0,他引:9
Persistence of five avian influenza viruses (AIVs) derived from four waterfowl species in Louisiana and representing five hemagglutinin and neuraminidase subtypes was determined in distilled water at 17 C and 28 C. Infectivity was determined over 60 days by microtiter endpoint titration. One AIV was tested over 91 days at 4 C. Linear regression models for these viruses predicted that an initial concentration of 1 x 10(6) TCID50/ml water could remain infective for up to 207 days at 17 C and up to 102 days at 28 C. Significant differences in slopes for AIV persistence models were detected between treatment temperatures and among viruses. Results suggest that these viruses are adapted to transmission on waterfowl wintering habitats. Results also suggest a potential risk associated with waterfowl and domestic poultry sharing a common water source. 相似文献