草地贪夜蛾核型多角体病毒对草地贪夜蛾的毒力及其侵染特征

为明确草地贪夜蛾核型多角体病毒（Spodoptera frugiperda multiple nucleopolyhedrovirus,SfMNPV）对草地贪夜蛾Spodoptera frugiperda 的毒力及其体内外侵染特性,于室内测定SfMNPV对草地贪夜蛾的毒力,采用苏木精-伊红（hematoxylin-eosin,HE）染色分析SfMNPV侵染后草地贪夜蛾的组织病理学特征,通过实时荧光定量PCR（real-time fluorescence quantitative PCR,qPCR）技术分析SfMNPV在草地贪夜蛾体内和Sf9细胞系中的复制特征。结果表明,SfMNPV对草地贪夜蛾2龄幼虫的LC₅₀和LT₅₀分别为7.39×10⁵ OBs/mL和3.82 d;SfMNPV可侵染草地贪夜蛾幼虫的真皮、气管、脂肪体和中肠等组织,未侵染肌肉组织;SfMNPV侵染96 h后草地贪夜蛾体内SfMNPV基因组拷贝数最大,为3.91×10⁶ 拷贝数/ng,SfMNPV侵染96 h后Sf9细胞培养上清液中SfMNPV基因组拷贝数最大,为2.08×10⁷ 拷贝数/mL。     

浅谈克服东魁杨梅大小年的关键技术

近几年来,我镇果农大量种植杨梅,经统计全镇共种植320.6亩.由于品种较杂,存在产量低、品质差,平均每亩可增产223.9kg左右,个别年份虽产量高但品质差,增产不增收的现状.     

RAPD标记鉴定果树芽变的可行性分析

在综合分析果树芽变的遗传特点以及RAPD标记技术的原理与存在问题的基础上，结合已有的研究报道，对RAPD标记应用于果树芽变鉴定中的可行性进行了探讨。对于不同的果树芽变类型，RAPD标记技术表现出不同的鉴定可行性。同时，对某些有关的问题提出了相应的建议。     

不同成熟度黄皮果实品质及贮藏特性的差异

黄皮Clausena lansium(Lour.)Skeels是我国南方原产的热带亚热带常绿果树。黄皮果实酸甜可口,风味独特,营养价值高且具有保健及药用价值,是深受人们喜爱的特产果品。近年来,我国黄皮栽培面积不断扩大,产量逐年提高。但黄皮采后极不耐贮藏,在自然条件下极易变软腐烂,1天后即明显     

Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

Marine collagen is an ideal material for tissue engineering due to its excellent biological properties. However, the limited mechanical properties and poor stability of marine collagen limit its application in tissue engineering. Here, collagen was extracted from the skin of tilapia (Oreochromis nilotica). Collagen-thermoplastic polyurethane (Col-TPU) fibrous membranes were prepared using tilapia collagen as a foundational material, and their physicochemical and biocompatibility were investigated. Fourier transform infrared spectroscopy results showed that thermoplastic polyurethane was successfully combined with collagen, and the triple helix structure of collagen was retained. X-ray diffraction and differential scanning calorimetry results showed relatively good compatibility between collagen and TPU.SEM results showed that the average diameter of the composite nanofiber membrane decreased with increasing thermoplastic polyurethane proportion. The mechanical evaluation and thermogravimetric analysis showed that the thermal stability and tensile properties of Col-TPU fibrous membranes were significantly improved with increasing TPU. Cytotoxicity experiments confirmed that fibrous membranes with different ratios of thermoplastic polyurethane content showed no significant toxicity to fibroblasts; Col-TPU fibrous membranes were conducive to the migration and adhesion of cells. Thus, these Col-TPU composite nanofiber membranes might be used as a potential biomaterial in tissue regeneration.     

Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.     

基于推拉理论的高校毕业生就业驱动机制研究

引入推拉理论分析了其在高校毕业生就业驱动机制中的适用性,并在剖析高校毕业生就业过程参与主体的基础上,从政府-高校-毕业生、政府-高校-劳动力市场、政府-毕业生-劳动力市场和高校-毕业生-劳动力市场等四个三维驱动系统分析,得出高校毕业生的就业,除了毕业生自身提高就业能力外,要构建毕业生、高校、政府、劳动力市场四位一体的就业驱动机制。     

一株惊纹地老虎幼虫颗粒体病毒的研究

1978年春,在新疆博湖从惊纹地老虎Agrotis exclamationis(Linné)(鳞翅目:夜蛾科)幼虫中发现一株病毒“Ae—02”,病毒颗粒体为卵形或卵圆形,大小为374～780×312～450nm,平均为578×412nm;病毒粒子杆状,两端钝圆,不弯曲或稍弯曲,大小为350～450×40～65nm,平均为395×50nm。该病毒对惊纹地老虎和黄地老虎A.segetum(Schiff.)幼虫致病力强。在日平均温度为24.2℃时,对1日龄惊纹地老虎幼虫感病19天后死亡率达96～100%;25天后10日龄幼虫死亡率达84～92%;对15日龄幼虫死亡率为73.96～80.96%。惊纹地老虎病毒“Ae—02”毒株,属杆状病毒科Baculoviridae,杆状病毒属Baculovirus,“B”亚组,定名为惊纹地老虎颗粒体病毒Agrotis excula-mationis granulosis virus,俗名鸣夜蛾颗粒体病毒,拉丁学名为Baculovirusexclamationis“B”,简称AeGV。     

大麦黄花叶病毒(BaYMV)机械接种的初步研究

 大麦黄花叶病毒的机械接种一般发病率较低,为提高其发病率,我们进行了缓冲液种类、用量,接种源种类,接种时苗龄,按种后苗培育时的温度、光照强度和时数等因素的研究。     

盐类对南方根结线虫迁移能力和侵入能力的影响

为探讨无机盐和有机盐对南方根结线虫的作用方式,在室内利用沙柱法和染色法测定了二者对南方根结线虫2龄幼虫迁移能力及侵入番茄根部能力的影响。结果表明,随着各盐处理浓度的增加,南方根结线虫2龄幼虫的迁移能力和侵入能力显著降低。在4μg/m L KSCN、C6H8O7、KCl、KH2PO4、KHCO3和Mn SO4·H2O沙柱中,15 d后2龄幼虫的平均迁移距离最小,分别为0、0.33、0.52、0.53、0.55和0.58 cm,而对照为0.94 cm。2龄幼虫在Ba NO3、K2HPO4、KHCO3、Cs Cl和KSCN各浓度沙柱中的平均迁移距离均小于0.94 cm。2μg/m L NH4NO3、KH2PO4、(NH4)2CO3、Cs Cl、(NH4)2SO4、Na SCN和NH4SCN处理15 d后,2龄幼虫对番茄根部的侵入率最低,分别为1%、2.33%、1.33%、2%、2%、1%和1.67%。表明对2龄幼虫侵入番茄根部抑制效果最明显的盐为NH4NO3、KH2PO4、(NH4)2CO3、Cs Cl、(NH4)2SO4、Na SCN和NH4SCN。