全文获取类型
收费全文 | 23436篇 |
免费 | 878篇 |
国内免费 | 1549篇 |
专业分类
林业 | 2496篇 |
农学 | 2344篇 |
基础科学 | 1207篇 |
3346篇 | |
综合类 | 5378篇 |
农作物 | 1522篇 |
水产渔业 | 1149篇 |
畜牧兽医 | 6030篇 |
园艺 | 808篇 |
植物保护 | 1583篇 |
出版年
2023年 | 216篇 |
2022年 | 553篇 |
2021年 | 691篇 |
2020年 | 679篇 |
2019年 | 632篇 |
2018年 | 544篇 |
2017年 | 817篇 |
2016年 | 691篇 |
2015年 | 914篇 |
2014年 | 870篇 |
2013年 | 903篇 |
2012年 | 1417篇 |
2011年 | 1418篇 |
2010年 | 1022篇 |
2009年 | 991篇 |
2008年 | 901篇 |
2007年 | 828篇 |
2006年 | 763篇 |
2005年 | 1447篇 |
2004年 | 1004篇 |
2003年 | 744篇 |
2002年 | 390篇 |
2001年 | 412篇 |
2000年 | 386篇 |
1999年 | 613篇 |
1998年 | 399篇 |
1997年 | 350篇 |
1996年 | 319篇 |
1995年 | 267篇 |
1994年 | 313篇 |
1993年 | 250篇 |
1992年 | 319篇 |
1991年 | 325篇 |
1990年 | 314篇 |
1989年 | 290篇 |
1988年 | 257篇 |
1987年 | 178篇 |
1986年 | 180篇 |
1985年 | 141篇 |
1984年 | 113篇 |
1979年 | 142篇 |
1975年 | 142篇 |
1974年 | 130篇 |
1973年 | 116篇 |
1972年 | 141篇 |
1971年 | 118篇 |
1969年 | 103篇 |
1968年 | 114篇 |
1967年 | 125篇 |
1966年 | 103篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
Evaluation of the cationic trypsinogen gene for potential mutations in miniature schnauzers with pancreatitis
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Micah A. Bishop J?rg M. Steiner Lisa E. Moore David A. Williams 《Canadian journal of veterinary research》2004,68(4):315-318
The purpose of this study was to evaluate the cationic trypsinogen gene in miniature schnauzers for possible mutations. Genetic mutations have been linked with hereditary pancreatitis in humans. Four miniature schnauzers were selected on the basis of a clinical history of pancreatitis. One healthy miniature schnauzer and 1 healthy mixed breed canine were enrolled as controls. DNA was extracted from these canines using a commercial kit. Primers were designed to amplify the entire canine cationic trypsinogen cDNA sequence. A polymerase chain reaction (PCR) was performed and products were purified and sequenced. All sequences were then compared. The healthy control canine, a healthy miniature schnauzer, and the 4 miniature schnauzers with pancreatitis showed identical sequences of the cationic trypsinogen gene to the published sequence. We conclude that, in contrast to humans with hereditary pancreatitis, mutations of the cationic trypsinogen gene do not play a major role in the genesis of pancreatitis in the miniature schnauzer. 相似文献
132.
Armando Garma-Avi?a 《Journal of veterinary diagnostic investigation》2004,16(6):585-587
An inexpensive sedimentation chamber to obtain cytologic specimens of cerebrospinal fluid (CSF) is described. The device, which has a total cost of about $5.00 can be built in few minutes. The device permits the cytologic study of specimens of CSF in clinics, where because of economic constraints, a cytocentrifuge is not available. The device permits the study of the CSF cells on either air-dried or wet smears even under field conditions, and the results obtained are consistent. Also, the device permits to retrieve the cell-free fluid for its use in chemical or immunologic procedures. 相似文献
133.
Yeen Ten Hwang Marie-Line Gentes Dennilyn L Parker Serge Larivière Fran?ois Messier 《Journal of zoo and wildlife medicine》2004,35(4):515-519
A total of 20 (14 females, six males) captive striped skunks (Mephitis mephitis) with miniature temperature dataloggers implanted free in the abdominal cavity were examined for reproductive performance and pathology because of implants. Eleven of 12 female skunks reproduced successfully 45.9+/-3.7 days after surgery to remove implanted dataloggers. The pregnancy rate of 91.7% (11/12) was much higher than other captive studies and was comparable with that of wild skunks. Eight striped skunks (six males, two females) that were euthanatized and necropsied after having implants in the abdominal cavity for 5 mo showed no apparent pathology associated with the implant. Implantation of the intraperitoneal devices did not lead to complications in abdominal tissues. Neither implant nor surgery affected reproduction. We conclude that implanted dataloggers can safely be used in physiologic studies of striped skunks or possibly other small carnivores in captive or field studies. 相似文献
134.
Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Roberto Hamer-Barrera Delfino Godínez V. Idalia Enríquez Sergio Vaca-Pacheco Rodrigo Martínez-Zú?iga Patricia Talamás-Rohana Francisco Suárez-Güemez Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract. 相似文献
135.
蚕粪中提取叶绿素铜钠盐的稳定性研究 总被引:10,自引:3,他引:10
以蚕粪为原料,95%的乙醇为溶剂提取叶绿素,并通过化学法制得叶绿素的铜纳盐。采用721分光光度计对叶绿素铜钠盐进行定性、定量测定,确定该色素测定系统中的最大波长为414nm。通过测量吸光值研究了几种常用食品添加剂对叶绿素铜钠盐稳定性的影响,并对其耐光、耐热、耐酸碱及耐氧化性、耐还原性进行探讨。结果表明:叶绿素铜钠盐在70℃以下稳定性较好;光照对叶绿素铜钠盐稳定性的影响较大;还原剂(Na2S2O3)对叶绿素铜钠盐稳定性的影响较大,氧化剂在短时间内对其稳定性的影响不大,但放置的时间不宜超过30min;叶绿素铜钠盐在pH值为6以上的溶液中较为稳定,且有机酸及单糖对其稳定性无明显影响。 相似文献
136.
植物育种的物理学方法 总被引:10,自引:0,他引:10
介绍了草原生态恢复中选育优良牧草种子的一些物理学方法,包括电场、磁场、物理辐射、激光、离子束和交变应力等在植物的生物学效应、诱变育种和转基因技术中的应用. 相似文献
137.
大豆多肽的性质及其研究进展 总被引:5,自引:0,他引:5
大豆多肽(Soy peptide)是大豆蛋白质经酶解作用后的多肽分子混合物。与大豆蛋白相比,大豆肽具有更好的理化性质,含有多种生理活性物质,在机体内具有更多种生理功能,近年来引起人们的广泛关注。 相似文献
138.
日粮中添加杜仲提取物对肉仔鸡生产性能的影响 总被引:2,自引:0,他引:2
选用825只艾维茵肉仔鸡,随机分为5组,每组5个重复,每个重复33只鸡。对照组饲喂基础日粮,试验组在基础日粮中分别添加1500,2000,2500g/t的杜仲提取物或50mg/kg的效美素,比较杜仲提取物对肉仔鸡生产性能及胴体品质的影响。试验分2个阶段(0~21d和21~42d),试验鸡自由采食和饮水。研究结果表明,1)日粮中添加杜仲提取物能降低采食量,提高肉仔鸡成活率,其中以添加1500g/t杜仲提取物效果较好。2)杜仲提取物能促进肌肉组织蛋白质的合成,降低体内脂肪的沉积,在一定程度上改善肉仔鸡胴体品质。 相似文献
139.
140.
在测定5类9种粗饲料及3个混合粗饲料的常规成分基础上,实测了它们的粗饲料分级指数(GI):大青山草地早熟禾3.4758、沙打旺3.3731、苜蓿1.7202、混粗C21.1797、青干草1.1029、混粗C10.7852、羊草0.5684、玉米青贮0.4152、玉米秸0.1985、谷草0.1728、混粗D0.0805、苏丹草0.0687,并就GI与饲料相对值(RFV)对粗饲料品质的分级进行了比较,表明GI要优于RFV,同时证明:GI还可以用于混合粗饲料的优化与牧草刈割期的确定。研究并建立了预测粗饲料代谢能(ME)的模型:ME(MJ/kg)=-11.7492 0.5959IVDM(D%)-0.0044[IVDM(D%)]2,其中IVDMD为体外干物质消化率。 相似文献