西藏生态餐厅建设概况与发展对策

近年来，随着小康社会建设和人民生活水平的不断提高，消费者越来越关注就餐环境、舒适程度、餐饮品种、科学营养等问题。就是在这种市场需求下，现代农业示范园区把餐饮活动引进温室内，在新、优、特蔬、果、花温室生产的基础上，迅速发展为生态餐厅。本文针对生态餐厅的建设概况及存在问题，提出今后发展方向及对策，供经营者参考。     

新式联体侧旋风道通风鸡舍的设计与建造

近些年受厄尔尼诺气候影响出现暖冬天气．这忽冷忽热的天气不仅使人类面临疫病的侵袭，对畜禽养殖也会产生不利影响。     

浅谈饲料企业差异化营销战略

随着饲料行业市场竞争的不断加剧，世界经济一体化的不断加强，运用价格战等传统手段进行竞争已经越来越难以在市场中取得比较优势。许多成熟的跨国公司往往运用差异化营销战略而不是价格战来获取竞争优势．既满足了消费者的需求．又扩大了市场占有率。     

饲料企业如何办一本高质量的企业内刊

近几年来．饲料企业的内刊如雨后春笋般地涌现出来．几乎稍微有点规模的饲料企业都有了内刊。虽然发行量大小不一，质量也良莠不齐，但众多的企业内刊和正规的行业传媒交相辉映．成为饲料界一道靓丽的风景线。     

喀斯特地区奶牛营养需求模型研究

选择贵州喀斯特地区中国荷斯坦奶牛48头,其中泌乳盛期、泌乳中期、泌乳末期及干奶期的奶牛各12头.将每个泌乳阶段的12头奶牛随机分为3组,分别饲喂不同营养水平的日粮,记录试验牛的日采食量、日产奶量、试验期间体重变化等数据.根据营养学原理,利用EXCEL回归分析,研究奶牛的维持需要量,构建营养需求模型.研究发现:DM(kg)=0.084 5W0.75+0.425FCM+(1.529 6+0.003 714×BW)×△WNER(kJ/d)=[424.40+2.5x(18-T)]Wa0.75+3 138×(0.4M+15×F%×M)+3 138K1×△WDCP(g/d)=3.20W0.75+M×DCP%,0.60+K2×△WCa(g/d)=0.072 5W+4.5×(0.4M+15×F%×M)+△W×20P(g/d)=0.050 513W+3×(0.4M+15×F%×M)+△W×13     

饲料加工工对营养物质利用率及畜禽生产性能的影响

高品质的饲料产品不仅取决于配方好坏、原材料的优劣,同时加工工艺也起着重要的作用.饲料在加工过程中会发生一系列物理、化学变化,从而影响饲料中各种营养成分的利用率,以及动物的生产性能.文中从饲料粉碎粒度、混合均匀度、膨化与否、制粒效果方面对营养物质利用率和畜禽生产性能的影响作一综述.     

茄子早熟育种研究Ⅱ选择指数与早熟性选择

研究结果表明,由性状果实最大周长,果实体积,门茄显蕾期,单株产量等性状构成的选择指数较好;进一步进行遗传相关分析结果认为,应用选择指数时应注意果实体积这个性状,单株产量是一个重要的辅助性状,门茄显蕾期是一个限制性因子。应用选择指数对品种评价结果认为,早熟性较好的品种有:天津早茄、西安绿茄、二民、七叶茄、白线茄和汉中荷包。     

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)